Ci Ji (CJ) Lim
@cijilim.bsky.social
Associate Professor @ UW-Madison Biochemistry. www.limlab.org
Telomeres, Cryo-EM, Single-molecule Biophysics. Opinions are my own. 🇸🇬
Telomeres, Cryo-EM, Single-molecule Biophysics. Opinions are my own. 🇸🇬
Pinned
Human RPA is an essential telomerase processivity factor for maintaining telomeres
Telomerase counteracts telomere shortening by repeatedly adding DNA repeats to chromosome ends. We identified the replication protein A (RPA) heterotrimer as a telomerase processivity factor critical ...
www.science.org
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Reposted by Ci Ji (CJ) Lim
PSA for people with NIH grant periods starting January 1st (including most NIGMS MIRAs): your RPPR is due Saturday, but they haven’t sent out the usual automated reminders, presumably due to the shutdown
November 11, 2025 at 1:20 AM
PSA for people with NIH grant periods starting January 1st (including most NIGMS MIRAs): your RPPR is due Saturday, but they haven’t sent out the usual automated reminders, presumably due to the shutdown
Reposted by Ci Ji (CJ) Lim
Very well done story on 60 Minutes about the harms of grant terminations and freezes at Harvard with Joan Brugge, Don Engbar, David Liu, and a very compelling young cancer patient, now cured with Liu's technology.
Transcript and video here
www.cbsnews.com/news/researc...
Transcript and video here
www.cbsnews.com/news/researc...
Battle between Trump and universities hurting scientific research in need of federal funding
Federal research funding to universities has fueled breakthroughs for years. The White House is pressuring universities to align with the president's political agenda, or risk losing their funding.
www.cbsnews.com
November 10, 2025 at 1:03 AM
Very well done story on 60 Minutes about the harms of grant terminations and freezes at Harvard with Joan Brugge, Don Engbar, David Liu, and a very compelling young cancer patient, now cured with Liu's technology.
Transcript and video here
www.cbsnews.com/news/researc...
Transcript and video here
www.cbsnews.com/news/researc...
New work from Tahirov x Lim collaboration! @qixianghe.bsky.social from my lab contributed the cryo-EM structures for this work. We are excited to help explain how anti-HSV drugs work and to guide their future development.
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
Structural basis of herpesvirus helicase-primase inhibition by pritelivir and amenamevir
Structural studies of HSV-1 helicase-primase revealed how pritelivir and amenamevir bind and block its helicase activity.
www.science.org
November 10, 2025 at 12:14 AM
New work from Tahirov x Lim collaboration! @qixianghe.bsky.social from my lab contributed the cryo-EM structures for this work. We are excited to help explain how anti-HSV drugs work and to guide their future development.
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
Reposted by Ci Ji (CJ) Lim
Work published today in @natbiotech.nature.com from Arc’s Luke Gilbert and Patrick Hsu labs presents a new way to insert large DNA sequences into the genome using engineered recombinases that don’t require DNA cutting or rely on the cell's repair machinery.
November 6, 2025 at 5:46 PM
Work published today in @natbiotech.nature.com from Arc’s Luke Gilbert and Patrick Hsu labs presents a new way to insert large DNA sequences into the genome using engineered recombinases that don’t require DNA cutting or rely on the cell's repair machinery.
Reposted by Ci Ji (CJ) Lim
🧬 List of 2026 DNA repair and genome stability conferences, now updated. Please let me know if there is anything missing!
docs.google.com/spreadsheets...
docs.google.com/spreadsheets...
DNA REPAIR/GENOME STABILITY CONFERENCES
docs.google.com
November 9, 2025 at 10:33 PM
🧬 List of 2026 DNA repair and genome stability conferences, now updated. Please let me know if there is anything missing!
docs.google.com/spreadsheets...
docs.google.com/spreadsheets...
Reposted by Ci Ji (CJ) Lim
We’re hiring! 🚨
Assistant Professor (TT) in Theoretical or Computational Biological Physics @univmiami.bsky.social 😀
Come build the future of interdisciplinary biophysics with us!
Apply by Dec 15 → tinyurl.com/5n9bk836
#Biophysics #PhysicsJobs #AcademicJobs #UMiami
Assistant Professor (TT) in Theoretical or Computational Biological Physics @univmiami.bsky.social 😀
Come build the future of interdisciplinary biophysics with us!
Apply by Dec 15 → tinyurl.com/5n9bk836
#Biophysics #PhysicsJobs #AcademicJobs #UMiami
November 8, 2025 at 5:54 PM
We’re hiring! 🚨
Assistant Professor (TT) in Theoretical or Computational Biological Physics @univmiami.bsky.social 😀
Come build the future of interdisciplinary biophysics with us!
Apply by Dec 15 → tinyurl.com/5n9bk836
#Biophysics #PhysicsJobs #AcademicJobs #UMiami
Assistant Professor (TT) in Theoretical or Computational Biological Physics @univmiami.bsky.social 😀
Come build the future of interdisciplinary biophysics with us!
Apply by Dec 15 → tinyurl.com/5n9bk836
#Biophysics #PhysicsJobs #AcademicJobs #UMiami
Reposted by Ci Ji (CJ) Lim
CryoSift is now out @actacrystf.iucr.org doi.org/10.1107/S205... Thanks to all involved. Happy processing!
November 7, 2025 at 4:58 PM
CryoSift is now out @actacrystf.iucr.org doi.org/10.1107/S205... Thanks to all involved. Happy processing!
Reposted by Ci Ji (CJ) Lim
We're hiring in history of science at UW Madison! TT Assistant Professorship with a focus on water. Joint appointment between the History and Integrated Liberal Studies depts, and part of a university-wide hiring cluster on earth/sustainability science. jobs.wisc.edu/jobs/profess... #STS #HSMT
Professor of History - Madison, Wisconsin, United States
Current Employees: If you are currently employed at any of the Universities of Wisconsin, log in to Workday to apply through the internal application process.Job Category:FacultyEmployment Type:Regula...
jobs.wisc.edu
November 6, 2025 at 7:37 PM
We're hiring in history of science at UW Madison! TT Assistant Professorship with a focus on water. Joint appointment between the History and Integrated Liberal Studies depts, and part of a university-wide hiring cluster on earth/sustainability science. jobs.wisc.edu/jobs/profess... #STS #HSMT
Reposted by Ci Ji (CJ) Lim
James Watson has died www.nytimes.com/2025/11/07/s...
James Watson, Co-Discoverer of the Structure of DNA, Is Dead at 97
www.nytimes.com
November 7, 2025 at 8:00 PM
James Watson has died www.nytimes.com/2025/11/07/s...
Reposted by Ci Ji (CJ) Lim
Excellent #postdoc opportunity available in @sjorsscheres.bsky.social’ group, working on new image processing algorithms for #cryoEM structure determination in their #RELION open-source software.
www.nature.com/naturecareer...
Apply by 25 NOV
#PostdocJobs #ScienceJobs
www.nature.com/naturecareer...
Apply by 25 NOV
#PostdocJobs #ScienceJobs
November 6, 2025 at 3:46 PM
Excellent #postdoc opportunity available in @sjorsscheres.bsky.social’ group, working on new image processing algorithms for #cryoEM structure determination in their #RELION open-source software.
www.nature.com/naturecareer...
Apply by 25 NOV
#PostdocJobs #ScienceJobs
www.nature.com/naturecareer...
Apply by 25 NOV
#PostdocJobs #ScienceJobs
Reposted by Ci Ji (CJ) Lim
IT'S HAPPENING! 💥 I'm psyched to launch the collaboration between @qedscience.bsky.social & @openrxiv.bsky.social @biorxivpreprint.bsky.social! Preprint + q.e.d = your science is out there, and anyone can appreciate it. Let's care about making discoveries, and not on “getting published” (1/3) 👇
November 6, 2025 at 2:49 PM
IT'S HAPPENING! 💥 I'm psyched to launch the collaboration between @qedscience.bsky.social & @openrxiv.bsky.social @biorxivpreprint.bsky.social! Preprint + q.e.d = your science is out there, and anyone can appreciate it. Let's care about making discoveries, and not on “getting published” (1/3) 👇
Reposted by Ci Ji (CJ) Lim
Our latest paper has just been published in Cell!
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
November 5, 2025 at 5:20 PM
Our latest paper has just been published in Cell!
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
Reposted by Ci Ji (CJ) Lim
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
November 5, 2025 at 4:23 PM
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
Reposted by Ci Ji (CJ) Lim
Ready for a transformative research journey? 🚀
Join the vibrant, interdisciplinary PhD programmes at NTU College of Science in Singapore! We offer fully funded scholarships.
Hear from our students and faculty in the video below 👇
youtu.be/JeZHiXu3fgE
Join the vibrant, interdisciplinary PhD programmes at NTU College of Science in Singapore! We offer fully funded scholarships.
Hear from our students and faculty in the video below 👇
youtu.be/JeZHiXu3fgE
NTU College of Science PhD opportunities
YouTube video by College of Science NTU
youtu.be
November 5, 2025 at 3:00 AM
Ready for a transformative research journey? 🚀
Join the vibrant, interdisciplinary PhD programmes at NTU College of Science in Singapore! We offer fully funded scholarships.
Hear from our students and faculty in the video below 👇
youtu.be/JeZHiXu3fgE
Join the vibrant, interdisciplinary PhD programmes at NTU College of Science in Singapore! We offer fully funded scholarships.
Hear from our students and faculty in the video below 👇
youtu.be/JeZHiXu3fgE
Reposted by Ci Ji (CJ) Lim
November 4, 2025 at 3:48 PM
Reposted by Ci Ji (CJ) Lim
Have a look at this Tools of the Trade Article I wrote for @natrevmcb.nature.com on decoding #ubiquitin signals inside cells using UbiREAD!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
ICYMI: New Online! Decoding ubiquitin signals inside cells
Decoding ubiquitin signals inside cells
Nature Reviews Molecular Cell Biology, Published online: 31 October 2025; doi:10.1038/s41580-025-00919-zIn this Tools of the Trade article, Kiss (Schulman lab) discusses the development of UbiREAD, a method that delivers in vitro-ubiquitinated protein reporters into cells to systematically assess how different ubiquitin chain configurations affect protein stability and degradation.
bit.ly
November 4, 2025 at 10:04 AM
Have a look at this Tools of the Trade Article I wrote for @natrevmcb.nature.com on decoding #ubiquitin signals inside cells using UbiREAD!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Reposted by Ci Ji (CJ) Lim
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
October 31, 2025 at 9:24 AM
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
Reposted by Ci Ji (CJ) Lim
Nature suggests you use their "Manuscript Adviser" bot to get advice before submitting
I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
November 3, 2025 at 1:55 PM
Nature suggests you use their "Manuscript Adviser" bot to get advice before submitting
I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
I uploaded the classic Watson & Crick paper about DNA structure, and the Adviser had this to say about one of the greatest paper endings of the century:
Reposted by Ci Ji (CJ) Lim
We're counting down to the 2025 WARF Innovation Awards! Meet our second nominee, a computer scientist with a system that helps computers use GPUs more efficiently, making AI and other apps work better. #WisconsinInnovates
Dynamic GPU scheduling boosts AI performance
A system designed by Ming Liu, assistant professor of computer science, improves how computers manage powerful GPUs by dynamically sharing and scheduling resources as tasks change. This means AI programs and other heavy-duty applications can run more smoothly and quickly. The technology adapts in real time to make the best use of available GPU power, helping businesses and researchers get more from their hardware. It supports flexible, scalable computing for today’s complex and fast-changing workloads.
Music: Bensound.com/royalty-free-music
Artist: : Lunar Years
youtu.be
November 3, 2025 at 9:48 PM
We're counting down to the 2025 WARF Innovation Awards! Meet our second nominee, a computer scientist with a system that helps computers use GPUs more efficiently, making AI and other apps work better. #WisconsinInnovates
Reposted by Ci Ji (CJ) Lim
Single-molecule friends!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
2026 Single Molecule Approaches to Biology Conference GRC
The 2026 Gordon Research Conference on Single Molecule Approaches to Biology will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.
www.grc.org
November 2, 2025 at 7:23 PM
Single-molecule friends!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
Reposted by Ci Ji (CJ) Lim
🧬🧪Research Technician Position Open🧬🧪
HHMI, UT Austin. Biochemistry/Biophysics- Assist recombinant protein expression, purification and structural analysis (protein NMR and CryoEM) to uncover the mechanisms of cell fate specification in plants.
hhmi.wd1.myworkdayjobs.com/en-US/Extern...
HHMI, UT Austin. Biochemistry/Biophysics- Assist recombinant protein expression, purification and structural analysis (protein NMR and CryoEM) to uncover the mechanisms of cell fate specification in plants.
hhmi.wd1.myworkdayjobs.com/en-US/Extern...
Research Technician - Torii Lab
Primary Work Address: 2500 Speedway, Austin, TX, 78712 Current HHMI Employees, click here to apply via your Workday account. About the Lab We are currently seeking a proactive Research Technician to s...
hhmi.wd1.myworkdayjobs.com
November 1, 2025 at 7:41 PM
🧬🧪Research Technician Position Open🧬🧪
HHMI, UT Austin. Biochemistry/Biophysics- Assist recombinant protein expression, purification and structural analysis (protein NMR and CryoEM) to uncover the mechanisms of cell fate specification in plants.
hhmi.wd1.myworkdayjobs.com/en-US/Extern...
HHMI, UT Austin. Biochemistry/Biophysics- Assist recombinant protein expression, purification and structural analysis (protein NMR and CryoEM) to uncover the mechanisms of cell fate specification in plants.
hhmi.wd1.myworkdayjobs.com/en-US/Extern...
Reposted by Ci Ji (CJ) Lim
Socioeconomic roots of academic faculty
Tenure-track faculty are up to 25 times more likely to have a parent with a Ph.D. Moreover, this rate nearly doubles at prestigious universities and is stable across the past 50 years.
#AcademicSky
Tenure-track faculty are up to 25 times more likely to have a parent with a Ph.D. Moreover, this rate nearly doubles at prestigious universities and is stable across the past 50 years.
#AcademicSky
Socioeconomic roots of academic faculty - Nature Human Behaviour
Studying socioeconomic backgrounds and intergenerational transmission in the US academia, Morgan et al. find that faculty have a parent with a Ph.D. degree a striking 25 times more often than the gene...
www.nature.com
November 1, 2025 at 3:53 PM
Socioeconomic roots of academic faculty
Tenure-track faculty are up to 25 times more likely to have a parent with a Ph.D. Moreover, this rate nearly doubles at prestigious universities and is stable across the past 50 years.
#AcademicSky
Tenure-track faculty are up to 25 times more likely to have a parent with a Ph.D. Moreover, this rate nearly doubles at prestigious universities and is stable across the past 50 years.
#AcademicSky
Reposted by Ci Ji (CJ) Lim
please look here for more information about a postdoc position careers.mskcc.org/vacancies/93...
93229-Postdoctoral Researcher – Laboratory of Max Wilkinson | Memorial Sloan Kettering Cancer Center
careers.mskcc.org
October 31, 2025 at 7:12 PM
please look here for more information about a postdoc position careers.mskcc.org/vacancies/93...
Reposted by Ci Ji (CJ) Lim
Excited to share my first PhD student’s @biorxivpreprint.bsky.social!
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
November 1, 2025 at 5:56 AM
Excited to share my first PhD student’s @biorxivpreprint.bsky.social!
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Reposted by Ci Ji (CJ) Lim
The Wilkinson Lab is open for science! @mskcancercenter.bsky.social
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
Wilkinson Lab
We discover and study reverse transcriptases
wilkinsonlab.bio
October 31, 2025 at 7:00 PM
The Wilkinson Lab is open for science! @mskcancercenter.bsky.social
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)