Ci Ji (CJ) Lim
@cijilim.bsky.social
Associate Professor @ UW-Madison Biochemistry. www.limlab.org
Telomeres, Cryo-EM, Single-molecule Biophysics. Opinions are my own. 🇸🇬
Telomeres, Cryo-EM, Single-molecule Biophysics. Opinions are my own. 🇸🇬
Pinned
Ci Ji (CJ) Lim
@cijilim.bsky.social
· Oct 30
Human RPA is an essential telomerase processivity factor for maintaining telomeres
Telomerase counteracts telomere shortening by repeatedly adding DNA repeats to chromosome ends. We identified the replication protein A (RPA) heterotrimer as a telomerase processivity factor critical ...
www.science.org
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Reposted by Ci Ji (CJ) Lim
Exciting news! The lab is moving to Colorado at @cuanschutz.bsky.social 🎉
We’ll be recruiting at all levels—stay tuned!
We’ll be recruiting at all levels—stay tuned!
February 3, 2026 at 8:46 PM
Exciting news! The lab is moving to Colorado at @cuanschutz.bsky.social 🎉
We’ll be recruiting at all levels—stay tuned!
We’ll be recruiting at all levels—stay tuned!
Reposted by Ci Ji (CJ) Lim
Based in the #Americas? @embo.org Lab #Leadership live online training for #scientists on 17-20 March (NY time). Take time to focus on, and tune up, your interpersonal skills and discover tools to support you and your team's development. Sign up now: www.embolableadership.org/course/embol...
February 3, 2026 at 8:22 AM
Based in the #Americas? @embo.org Lab #Leadership live online training for #scientists on 17-20 March (NY time). Take time to focus on, and tune up, your interpersonal skills and discover tools to support you and your team's development. Sign up now: www.embolableadership.org/course/embol...
Reposted by Ci Ji (CJ) Lim
H3K79me2/3 is sufficient to antagonise PRC1 www.nature.com/articles/s41...
DOT1L provides transcriptional memory through PRC1.1 antagonism - Nature Cell Biology
Neville, Ferguson et al. show that non-canonical Polycomb repressive complex 1.1-mediated gene silencing is antagonized by DOT1L and is required for the therapeutic efficacy of Menin and DOT1L inhibit...
www.nature.com
February 3, 2026 at 10:30 PM
H3K79me2/3 is sufficient to antagonise PRC1 www.nature.com/articles/s41...
Reposted by Ci Ji (CJ) Lim
My outstanding postdoc is looking for a research job in Albany, NY. Please forward all leads and RT!
February 3, 2026 at 10:14 PM
My outstanding postdoc is looking for a research job in Albany, NY. Please forward all leads and RT!
Reposted by Ci Ji (CJ) Lim
📣 We are accepting applications for the 2026 Boston Bioimage Analysis Course (BoBiAC): bobiac.github.io! Join us this July at Harvard Medical School for a 6-day intensive hands-on course to learn bioimage analysis with Python!
Apply by May 18th! No prior Python experience required!
🧫->🔬->💻->📊
Apply by May 18th! No prior Python experience required!
🧫->🔬->💻->📊
February 3, 2026 at 9:36 PM
📣 We are accepting applications for the 2026 Boston Bioimage Analysis Course (BoBiAC): bobiac.github.io! Join us this July at Harvard Medical School for a 6-day intensive hands-on course to learn bioimage analysis with Python!
Apply by May 18th! No prior Python experience required!
🧫->🔬->💻->📊
Apply by May 18th! No prior Python experience required!
🧫->🔬->💻->📊
Reposted by Ci Ji (CJ) Lim
📣 The 2026 Midwest DNA Repair Symposium is coming to Kansas City (registration is open)
🗓 May 30–31, 2026
✨ Talks will be selected from abstracts, with a strong focus on trainees and new investigators.
Please RT & share with your labs!
🔗 sites.google.com/view/kumc-mi...
🗓 May 30–31, 2026
✨ Talks will be selected from abstracts, with a strong focus on trainees and new investigators.
Please RT & share with your labs!
🔗 sites.google.com/view/kumc-mi...
February 3, 2026 at 8:41 PM
📣 The 2026 Midwest DNA Repair Symposium is coming to Kansas City (registration is open)
🗓 May 30–31, 2026
✨ Talks will be selected from abstracts, with a strong focus on trainees and new investigators.
Please RT & share with your labs!
🔗 sites.google.com/view/kumc-mi...
🗓 May 30–31, 2026
✨ Talks will be selected from abstracts, with a strong focus on trainees and new investigators.
Please RT & share with your labs!
🔗 sites.google.com/view/kumc-mi...
Reposted by Ci Ji (CJ) Lim
Unquestionably the result of combined effort from all current and former lab members, support from colleagues @CSU and @CU, and support from @hhmi-science.bsky.social and NIH over decades. A heartfelt 🙏 to my nominators and of course @vilcekfoundation.bsky.social.
vilcek.org/prizes/prize...
vilcek.org/prizes/prize...
Karolin Luger - Vilcek Foundation
Karolin Luger's research led to the capture of a high resolution image of chromatin, resulting in the development of innovative drug treatments for diseases like cancer.
vilcek.org
February 2, 2026 at 11:37 PM
Unquestionably the result of combined effort from all current and former lab members, support from colleagues @CSU and @CU, and support from @hhmi-science.bsky.social and NIH over decades. A heartfelt 🙏 to my nominators and of course @vilcekfoundation.bsky.social.
vilcek.org/prizes/prize...
vilcek.org/prizes/prize...
Big congratulations to Karolin!!! 👏🏻🤩
February 2, 2026 at 11:21 PM
Big congratulations to Karolin!!! 👏🏻🤩
Reposted by Ci Ji (CJ) Lim
This just in. So honored and grateful...
We're proud to join the Vilcek Foundation in recognizing HHMI Investigator Karolin Luger, whose dedication & groundbreaking nucleosome research has led to the development of innovative new drug treatments, including cancer medicines. hhmi.news/49VciEd @vilcekfoundation.bsky.social
February 2, 2026 at 6:24 PM
This just in. So honored and grateful...
Reposted by Ci Ji (CJ) Lim
Check out NASA’s new press release on how we recreated a molecule used by some of Earth’s earliest life forms, helping us understand how life began here and how we might spot signs of life on other planets! Big congrats to first author, @hollyrucker.bsky.social!
science.nasa.gov/science-rese...
science.nasa.gov/science-rese...
Resurrecting Ancient Enzymes in NASA's Search for Life Beyond Earth - NASA Science
NASA-supported scientists have resurrected an enzyme first used by organisms on Earth 3.2-billion years ago and, in the process, have validated a chemical
science.nasa.gov
January 30, 2026 at 9:16 PM
Check out NASA’s new press release on how we recreated a molecule used by some of Earth’s earliest life forms, helping us understand how life began here and how we might spot signs of life on other planets! Big congrats to first author, @hollyrucker.bsky.social!
science.nasa.gov/science-rese...
science.nasa.gov/science-rese...
Reposted by Ci Ji (CJ) Lim
A rule I follow when reviewing cryo-EM manuscripts: if a claim relies on the orientation or interactions of a side chain, the evidence supporting the claim (ie the map density) must be shown.
In general, reviewers are responsible for ensuring claims in manuscripts are supported by data.
In general, reviewers are responsible for ensuring claims in manuscripts are supported by data.
January 30, 2026 at 2:04 PM
A rule I follow when reviewing cryo-EM manuscripts: if a claim relies on the orientation or interactions of a side chain, the evidence supporting the claim (ie the map density) must be shown.
In general, reviewers are responsible for ensuring claims in manuscripts are supported by data.
In general, reviewers are responsible for ensuring claims in manuscripts are supported by data.
Reposted by Ci Ji (CJ) Lim
Happy new year! We have some exciting news, the registration and abstract submissions for the 22nd Nucleic Acids Forum 2026 are now open! Join us on the 10th of July at Burlington House!
Hurry before it sells out! 🧬
www.rsc.org/events/detai...
Hurry before it sells out! 🧬
www.rsc.org/events/detai...
22nd RSC Nucleic Acids Forum – 2026
The Royal Society of Chemistry, Burlington House, Piccadilly, Piccadilly, London, W1J 0BA, United Kingdom
www.rsc.org
January 29, 2026 at 9:05 AM
Happy new year! We have some exciting news, the registration and abstract submissions for the 22nd Nucleic Acids Forum 2026 are now open! Join us on the 10th of July at Burlington House!
Hurry before it sells out! 🧬
www.rsc.org/events/detai...
Hurry before it sells out! 🧬
www.rsc.org/events/detai...
Reposted by Ci Ji (CJ) Lim
The IKI at Ben-Gurion University is looking for a Staff Scientist to join our CryoEM Unit.
If you’re passionate about #spa and #cryoET, this is a rare opportunity to work in an amazing environment with top-tier science and even better collaborators (myself […]
[Original post on tooot.im]
If you’re passionate about #spa and #cryoET, this is a rare opportunity to work in an amazing environment with top-tier science and even better collaborators (myself […]
[Original post on tooot.im]
January 29, 2026 at 4:24 AM
The IKI at Ben-Gurion University is looking for a Staff Scientist to join our CryoEM Unit.
If you’re passionate about #spa and #cryoET, this is a rare opportunity to work in an amazing environment with top-tier science and even better collaborators (myself […]
[Original post on tooot.im]
If you’re passionate about #spa and #cryoET, this is a rare opportunity to work in an amazing environment with top-tier science and even better collaborators (myself […]
[Original post on tooot.im]
Reposted by Ci Ji (CJ) Lim
Make your gene knockouts more efficient with multiplexed Cas12a sgRNAs. Our new paper is out now, with tools available from @addgene.bsky.social , www.plasmids.eu and @vdrc-flies.bsky.social.
www.nature.com/articles/s41...
#CRISPR #geneediting #Drosophila 🧪🧬✂️🔬🪰
Summary 🧵 below.
www.nature.com/articles/s41...
#CRISPR #geneediting #Drosophila 🧪🧬✂️🔬🪰
Summary 🧵 below.
January 29, 2026 at 9:26 AM
Make your gene knockouts more efficient with multiplexed Cas12a sgRNAs. Our new paper is out now, with tools available from @addgene.bsky.social , www.plasmids.eu and @vdrc-flies.bsky.social.
www.nature.com/articles/s41...
#CRISPR #geneediting #Drosophila 🧪🧬✂️🔬🪰
Summary 🧵 below.
www.nature.com/articles/s41...
#CRISPR #geneediting #Drosophila 🧪🧬✂️🔬🪰
Summary 🧵 below.
Congratulations to Bret and the rest of the authors!!
January 28, 2026 at 1:33 AM
Congratulations to Bret and the rest of the authors!!
Reposted by Ci Ji (CJ) Lim
Jane Richardson was born #OTD in 1941
+ Developed the Richardson (ribbon) diagram to represent proteins' 3D structure (becoming a standard representation for protein structures)
+ MacArthur Fellow, 1985
+ Elected, Nat'l Academy of Sciences, 1991
+ President, Biophysical Society, 2012
#WomenInSTEM
+ Developed the Richardson (ribbon) diagram to represent proteins' 3D structure (becoming a standard representation for protein structures)
+ MacArthur Fellow, 1985
+ Elected, Nat'l Academy of Sciences, 1991
+ President, Biophysical Society, 2012
#WomenInSTEM
January 26, 2026 at 12:06 AM
Jane Richardson was born #OTD in 1941
+ Developed the Richardson (ribbon) diagram to represent proteins' 3D structure (becoming a standard representation for protein structures)
+ MacArthur Fellow, 1985
+ Elected, Nat'l Academy of Sciences, 1991
+ President, Biophysical Society, 2012
#WomenInSTEM
+ Developed the Richardson (ribbon) diagram to represent proteins' 3D structure (becoming a standard representation for protein structures)
+ MacArthur Fellow, 1985
+ Elected, Nat'l Academy of Sciences, 1991
+ President, Biophysical Society, 2012
#WomenInSTEM
Congratulations to Ahmad and the rest of the authors!!!
January 28, 2026 at 1:29 AM
Congratulations to Ahmad and the rest of the authors!!!
Reposted by Ci Ji (CJ) Lim
Our paper on the structural and functional basis of the human dimeric OS9–SEL1L–HRD1 ERAD complex, together with @qilabuva.bsky.social, is now out! Huge congratulations to Leo, Emir, and Elina!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
January 27, 2026 at 8:24 PM
Our paper on the structural and functional basis of the human dimeric OS9–SEL1L–HRD1 ERAD complex, together with @qilabuva.bsky.social, is now out! Huge congratulations to Leo, Emir, and Elina!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Beautiful work by Kelly and her team! Congratulations to Kelly, Sebastian, and the rest of the authors! Looking forward to reading this paper! 🤩
January 24, 2026 at 7:06 PM
Beautiful work by Kelly and her team! Congratulations to Kelly, Sebastian, and the rest of the authors! Looking forward to reading this paper! 🤩
Reposted by Ci Ji (CJ) Lim
Excited to share our latest work on dissecting the mechanism of processive telomeric DNA synthesis by telomerase. Led by amazing PhD student Sebastian Balch in collaboration with lab members,@automnenine.bsky.social @rachael-kretsch.bsky.social,@rdaslab.bsky.social.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structures of nucleotide-bound human telomerase at several steps of its telomeric DNA repeat addition cycle - Nature Communications
To maintain genome stability, the specialised reverse transcriptase telomerase processively adds telomeric DNA repeats to chromosome ends. Here the authors reported structures of human telomerase at t...
www.nature.com
January 23, 2026 at 6:57 AM
Excited to share our latest work on dissecting the mechanism of processive telomeric DNA synthesis by telomerase. Led by amazing PhD student Sebastian Balch in collaboration with lab members,@automnenine.bsky.social @rachael-kretsch.bsky.social,@rdaslab.bsky.social.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Congratulations to Jens, Mariia, and the rest of the authors!! Looking forward to reading this work.
January 24, 2026 at 7:05 PM
Congratulations to Jens, Mariia, and the rest of the authors!! Looking forward to reading this work.
Reposted by Ci Ji (CJ) Lim
New pre-print from the lab! Lead by Mariia Mikhova and in collaboration with Kefei Yu’s lab @msumgi.bsky.social we dug into the molecular mechanism of class switch recombination in B cells using simultaneous RNA and protein single-molecule imaging. 1/n
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
January 23, 2026 at 2:49 PM
New pre-print from the lab! Lead by Mariia Mikhova and in collaboration with Kefei Yu’s lab @msumgi.bsky.social we dug into the molecular mechanism of class switch recombination in B cells using simultaneous RNA and protein single-molecule imaging. 1/n
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Beautiful work! Congratulations to all authors! Looking forward to reading the paper in detail.
January 24, 2026 at 7:04 PM
Beautiful work! Congratulations to all authors! Looking forward to reading the paper in detail.
Reposted by Ci Ji (CJ) Lim
Cryo-EM structure reveals how influenza A virus NEP binds the viral polymerase at a regulatory hotspot, coordinating RNA synthesis and nuclear export. Fantastic collaboration with @loiccarrique.bsky.social and Jon Grimes. www.science.org/doi/10.1126/...
January 24, 2026 at 5:25 PM
Cryo-EM structure reveals how influenza A virus NEP binds the viral polymerase at a regulatory hotspot, coordinating RNA synthesis and nuclear export. Fantastic collaboration with @loiccarrique.bsky.social and Jon Grimes. www.science.org/doi/10.1126/...
Reposted by Ci Ji (CJ) Lim
