John Rubinstein
@johnrubinstein.bsky.social
CryoEM, Bioenergetics, V-ATPases (especially in synaptic vesicles and lysosomes), OxPhos (especially in mycobacteria as a TB drug target)
Scientist, The Hospital for Sick Children
Professor, U of Toronto
👨👩👧🔬🚲
rubinsteinlab.org
COI: structura.bio
Scientist, The Hospital for Sick Children
Professor, U of Toronto
👨👩👧🔬🚲
rubinsteinlab.org
COI: structura.bio
Pinned
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
Reposted by John Rubinstein
Reminder to register for an upcoming in-person #CryoSPARC workshop in Toronto, delivered by @sickkidsto.bsky.social and the #CryoSPARC team, in partnership with the Canadian #CryoEM Nexus!
Deadline fast approaching!
Deadline fast approaching!
Deadline approaching to register for the @structurabio.bsky.social & @sickkidsto.bsky.social CryoSPARC workshop in Toronto!
Open to all, this is a unique opportunity to learn CryoSPARC from the experts at Structura.
April 30/May, 2026.
For more information and to register:
tinyurl.com/y42pf4wy
Open to all, this is a unique opportunity to learn CryoSPARC from the experts at Structura.
April 30/May, 2026.
For more information and to register:
tinyurl.com/y42pf4wy
February 13, 2026 at 5:32 PM
Reminder to register for an upcoming in-person #CryoSPARC workshop in Toronto, delivered by @sickkidsto.bsky.social and the #CryoSPARC team, in partnership with the Canadian #CryoEM Nexus!
Deadline fast approaching!
Deadline fast approaching!
Deadline approaching to register for the @structurabio.bsky.social & @sickkidsto.bsky.social CryoSPARC workshop in Toronto!
Open to all, this is a unique opportunity to learn CryoSPARC from the experts at Structura.
April 30/May, 2026.
For more information and to register:
tinyurl.com/y42pf4wy
Open to all, this is a unique opportunity to learn CryoSPARC from the experts at Structura.
April 30/May, 2026.
For more information and to register:
tinyurl.com/y42pf4wy
February 13, 2026 at 4:40 PM
Deadline approaching to register for the @structurabio.bsky.social & @sickkidsto.bsky.social CryoSPARC workshop in Toronto!
Open to all, this is a unique opportunity to learn CryoSPARC from the experts at Structura.
April 30/May, 2026.
For more information and to register:
tinyurl.com/y42pf4wy
Open to all, this is a unique opportunity to learn CryoSPARC from the experts at Structura.
April 30/May, 2026.
For more information and to register:
tinyurl.com/y42pf4wy
Reposted by John Rubinstein
Female microbiologists are driving key breakthroughs in TB drug discovery- from uncovering the secrets of MTB persistence to discovering new therapeutics. They are reshaping the fight against the world’s deadliest bacterial pathogen. #WomenInScience #TBDrugDiscovery #Microbiology
February 12, 2026 at 12:45 AM
Female microbiologists are driving key breakthroughs in TB drug discovery- from uncovering the secrets of MTB persistence to discovering new therapeutics. They are reshaping the fight against the world’s deadliest bacterial pathogen. #WomenInScience #TBDrugDiscovery #Microbiology
Reposted by John Rubinstein
New publication alert 📢
Rouxjeane Venter, of CLIME recently co-authored an editorial with @tomayates.bsky.social and @drjesspotter.bsky.social , which unpacks the vast progress made in the fight against drug-resistant TB and highlights why these gains are now at risk! @ucl.ac.uk
Rouxjeane Venter, of CLIME recently co-authored an editorial with @tomayates.bsky.social and @drjesspotter.bsky.social , which unpacks the vast progress made in the fight against drug-resistant TB and highlights why these gains are now at risk! @ucl.ac.uk
February 9, 2026 at 9:35 AM
New publication alert 📢
Rouxjeane Venter, of CLIME recently co-authored an editorial with @tomayates.bsky.social and @drjesspotter.bsky.social , which unpacks the vast progress made in the fight against drug-resistant TB and highlights why these gains are now at risk! @ucl.ac.uk
Rouxjeane Venter, of CLIME recently co-authored an editorial with @tomayates.bsky.social and @drjesspotter.bsky.social , which unpacks the vast progress made in the fight against drug-resistant TB and highlights why these gains are now at risk! @ucl.ac.uk
Reposted by John Rubinstein
Dr. Kirsty Duncan - she lived every minute of her shortened life - and each was jam-packed. A huge loss. Gift link. www.theglobeandmail.com/gift/46242ee...
MP Kirsty Duncan became a crusader for safety in sport
A high achiever, she ran the Boston Marathon eight times, lifted weights and did triathlons, but it was her experience as a gymnast with body dysmorphia that inspired her to push for reforms to protec...
www.theglobeandmail.com
February 6, 2026 at 12:40 PM
Dr. Kirsty Duncan - she lived every minute of her shortened life - and each was jam-packed. A huge loss. Gift link. www.theglobeandmail.com/gift/46242ee...
A new frontier for our lab! After many months of development by postdoc @ranaabdelaziz.bsky.social (our resident medicinal chemistry - now also an excellent structural biologist) and the staff at the SickKids Drug Discovery platform, we're conducting our first proof-of-principle inhibitor screen.
February 5, 2026 at 3:22 AM
A new frontier for our lab! After many months of development by postdoc @ranaabdelaziz.bsky.social (our resident medicinal chemistry - now also an excellent structural biologist) and the staff at the SickKids Drug Discovery platform, we're conducting our first proof-of-principle inhibitor screen.
Reposted by John Rubinstein
In the latest @theglobeandmail.com feature, Sinai Health’s Dr. @danieljdrucker.bsky.social is recognized for the discovery that helped lead to Ozempic - and the ongoing breakthroughs unfolding at Sinai Health today.
Read the full story here:
Read the full story here:
The Canadian researcher whose discovery led to Ozempic is still making breakthroughs
Tackling obesity, diabetes only the beginning for GLP-1s, which have been found to reduce risks of other major diseases
www.theglobeandmail.com
February 3, 2026 at 3:30 PM
In the latest @theglobeandmail.com feature, Sinai Health’s Dr. @danieljdrucker.bsky.social is recognized for the discovery that helped lead to Ozempic - and the ongoing breakthroughs unfolding at Sinai Health today.
Read the full story here:
Read the full story here:
Reposted by John Rubinstein
Sign up now for the EMBO Workshop "The complexity of mycobacterial infections: From research to real-world impact" in #Paris, FR, 14–18 Sep 2026.
Registration by 25 Aug
Abstract submission by 15 May
https://meetings.embo.org/event/26-mycobacterial-infections
#EMBOComplexInfections #EMBOevents 🧪
Registration by 25 Aug
Abstract submission by 15 May
https://meetings.embo.org/event/26-mycobacterial-infections
#EMBOComplexInfections #EMBOevents 🧪
January 30, 2026 at 12:01 PM
Sign up now for the EMBO Workshop "The complexity of mycobacterial infections: From research to real-world impact" in #Paris, FR, 14–18 Sep 2026.
Registration by 25 Aug
Abstract submission by 15 May
https://meetings.embo.org/event/26-mycobacterial-infections
#EMBOComplexInfections #EMBOevents 🧪
Registration by 25 Aug
Abstract submission by 15 May
https://meetings.embo.org/event/26-mycobacterial-infections
#EMBOComplexInfections #EMBOevents 🧪
Reposted by John Rubinstein
Very excited for this in-person #CryoSPARC workshop in partnership with @sickkidsto.bsky.social and the Canadian #CryoEM Nexus! Register today!
January 30, 2026 at 6:00 PM
Very excited for this in-person #CryoSPARC workshop in partnership with @sickkidsto.bsky.social and the Canadian #CryoEM Nexus! Register today!
@structurabio.bsky.social and @sickkidsto.bsky.social CryoSPARC workshop in Toronto!
April 30/May 1st 2026.
Part of the Canadian Cryo-EM Nexus.
Please register here: sickkids.cventevents.com/event/a0aebb...
April 30/May 1st 2026.
Part of the Canadian Cryo-EM Nexus.
Please register here: sickkids.cventevents.com/event/a0aebb...
January 30, 2026 at 3:41 PM
@structurabio.bsky.social and @sickkidsto.bsky.social CryoSPARC workshop in Toronto!
April 30/May 1st 2026.
Part of the Canadian Cryo-EM Nexus.
Please register here: sickkids.cventevents.com/event/a0aebb...
April 30/May 1st 2026.
Part of the Canadian Cryo-EM Nexus.
Please register here: sickkids.cventevents.com/event/a0aebb...
Reposted by John Rubinstein
I am pleased to share our latest paper on the role of the alternative polyadenylation factor CFIM-1 (NUDT21) in C. elegans germline development. academic.oup.com/genetics/adv...
A stress-dependent postembryonic role for the core CPA factor CFIM-1 in germline integrity
Abstract. Post-transcriptional processing of pre-mRNAs by alternative polyadenylation (APA) generates a diversity of transcript isoforms at the 3’ untransl
academic.oup.com
January 29, 2026 at 4:45 PM
I am pleased to share our latest paper on the role of the alternative polyadenylation factor CFIM-1 (NUDT21) in C. elegans germline development. academic.oup.com/genetics/adv...
Reposted by John Rubinstein
🚀 CryoSPARC v5.0 BETA is here!
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
January 27, 2026 at 8:36 PM
🚀 CryoSPARC v5.0 BETA is here!
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
We’re excited to deploy another major #CryoSPARC release to help enable and accelerate #cryoEM data analysis. v5 has a redesigned underlying software system and many new features - highlights in thread!
Full changelog: cryosparc.com/updates/v5.0.0
Reposted by John Rubinstein
Gutted to learn of the passing of Dr Kirsty Duncan.
She was a tireless fighter for the people she served, not only her local community in Toronto but others, especially the science & research &
athletics communities too.
She championed equity, diversity, inclusion and made change happen.
🤍🙏🏻
She was a tireless fighter for the people she served, not only her local community in Toronto but others, especially the science & research &
athletics communities too.
She championed equity, diversity, inclusion and made change happen.
🤍🙏🏻
Former Liberal cabinet minister Kirsty Duncan dies at 59
OTTAWA — Former Liberal cabinet minister Kirsty Duncan has died at the age of 59 following a years-long battle with cancer. Born on Oct.
www.richmond-news.com
January 26, 2026 at 10:28 PM
Gutted to learn of the passing of Dr Kirsty Duncan.
She was a tireless fighter for the people she served, not only her local community in Toronto but others, especially the science & research &
athletics communities too.
She championed equity, diversity, inclusion and made change happen.
🤍🙏🏻
She was a tireless fighter for the people she served, not only her local community in Toronto but others, especially the science & research &
athletics communities too.
She championed equity, diversity, inclusion and made change happen.
🤍🙏🏻
Reposted by John Rubinstein
New Preprint alert! Excited to share our latest work on the #MAPKinases from stellar PhD student Jill von Velsen. First structures of the MAP2K MEK1 activating its substrate MAPK between 2.9 and 3.6A - amazing resolutions for such a small and mobile complex! www.biorxiv.org/content/10.6...
January 22, 2026 at 9:56 AM
New Preprint alert! Excited to share our latest work on the #MAPKinases from stellar PhD student Jill von Velsen. First structures of the MAP2K MEK1 activating its substrate MAPK between 2.9 and 3.6A - amazing resolutions for such a small and mobile complex! www.biorxiv.org/content/10.6...
Reposted by John Rubinstein
THE NEXT INSTALLMENT OF BIOLOGY OF MYOCBACTERIA GRC WILL BE IN 2028, NOT 2027. Exact date and location TBD. Sorry about the long wait, but we think it is better long term. PLEASE repost/tell your friends!
January 21, 2026 at 7:19 PM
THE NEXT INSTALLMENT OF BIOLOGY OF MYOCBACTERIA GRC WILL BE IN 2028, NOT 2027. Exact date and location TBD. Sorry about the long wait, but we think it is better long term. PLEASE repost/tell your friends!
Reposted by John Rubinstein
Job alert! Assistant professor position in the Department of Molecular Genetics (University of Toronto). Amazing department and city. jobs.utoronto.ca/job/Toronto-... Please share.
Assistant Professor - Virology
Assistant Professor - Virology
jobs.utoronto.ca
January 15, 2026 at 8:04 PM
Job alert! Assistant professor position in the Department of Molecular Genetics (University of Toronto). Amazing department and city. jobs.utoronto.ca/job/Toronto-... Please share.
Reposted by John Rubinstein
Western University is looking for a Canada Research Chair (CRC)
Tier 1 in Metabolomics. We are intent on building capacity in this system and the ideal candidate will help build infrastructure and mentor junior faculty.
Please see the job ad at: uwo.ca/facultyrelat...
Tier 1 in Metabolomics. We are intent on building capacity in this system and the ideal candidate will help build infrastructure and mentor junior faculty.
Please see the job ad at: uwo.ca/facultyrelat...
uwo.ca
January 12, 2026 at 9:12 PM
Western University is looking for a Canada Research Chair (CRC)
Tier 1 in Metabolomics. We are intent on building capacity in this system and the ideal candidate will help build infrastructure and mentor junior faculty.
Please see the job ad at: uwo.ca/facultyrelat...
Tier 1 in Metabolomics. We are intent on building capacity in this system and the ideal candidate will help build infrastructure and mentor junior faculty.
Please see the job ad at: uwo.ca/facultyrelat...
Like most 🇨🇦 scientists, I believe Canada Impact+ Research Chairs program is an idiotic use of $ in an underfunded ecosystem
That said, $ is now on the table and Unis can take or leave it
If you want to start or move your lab to 🇨🇦, you're about to see lots of opportunities with excellent funding
That said, $ is now on the table and Unis can take or leave it
If you want to start or move your lab to 🇨🇦, you're about to see lots of opportunities with excellent funding
January 10, 2026 at 2:14 PM
Like most 🇨🇦 scientists, I believe Canada Impact+ Research Chairs program is an idiotic use of $ in an underfunded ecosystem
That said, $ is now on the table and Unis can take or leave it
If you want to start or move your lab to 🇨🇦, you're about to see lots of opportunities with excellent funding
That said, $ is now on the table and Unis can take or leave it
If you want to start or move your lab to 🇨🇦, you're about to see lots of opportunities with excellent funding
Reposted by John Rubinstein
OPPORTUNITY: McGill Plant Science is looking for an expert in plant pathology, plant breeding, or horticulture in the context of the Canada Impact+ Research Chairs Program (senior established researcher).
Contact: www.mcgill.ca/plant/contact
Program: lnkd.in/eavfNu7e
Please re-post.
Contact: www.mcgill.ca/plant/contact
Program: lnkd.in/eavfNu7e
Please re-post.
LinkedIn
This link will take you to a page that’s not on LinkedIn
lnkd.in
January 7, 2026 at 9:24 PM
OPPORTUNITY: McGill Plant Science is looking for an expert in plant pathology, plant breeding, or horticulture in the context of the Canada Impact+ Research Chairs Program (senior established researcher).
Contact: www.mcgill.ca/plant/contact
Program: lnkd.in/eavfNu7e
Please re-post.
Contact: www.mcgill.ca/plant/contact
Program: lnkd.in/eavfNu7e
Please re-post.
Reposted by John Rubinstein
Very pleased to be on the organizing committee of the EMBO course Structural Characterization of Macromolecular Complexes in 2026! We have a fantastic line up of speakers and tutors, please share widely!
👉apply here: www.embl.org/about/info/c...
👉apply here: www.embl.org/about/info/c...
January 9, 2026 at 12:38 PM
Very pleased to be on the organizing committee of the EMBO course Structural Characterization of Macromolecular Complexes in 2026! We have a fantastic line up of speakers and tutors, please share widely!
👉apply here: www.embl.org/about/info/c...
👉apply here: www.embl.org/about/info/c...
I'm looking forward to speaking at this course (and finally seeing my PhD-labmate @mattbowler.bsky.social's institute in Grenoble)!
Very pleased to be on the organizing committee of the EMBO course Structural Characterization of Macromolecular Complexes in 2026! We have a fantastic line up of speakers and tutors, please share widely!
👉apply here: www.embl.org/about/info/c...
👉apply here: www.embl.org/about/info/c...
January 9, 2026 at 6:19 PM
I'm looking forward to speaking at this course (and finally seeing my PhD-labmate @mattbowler.bsky.social's institute in Grenoble)!
Reposted by John Rubinstein
Congrats to our former summer student🥳
January 9, 2026 at 2:31 PM
Congrats to our former summer student🥳
Congratulations to the new Doctor of Philosophy @courbongautier.bsky.social on a spectacular PhD defense following a groundbreaking PhD!
Thanks to external examiner Damian Ekiert from JHU and the rest of the examining committee.
Next stop, Oxford University for a postdoctoral fellowship.
🇫🇷🇨🇦🇬🇧
Thanks to external examiner Damian Ekiert from JHU and the rest of the examining committee.
Next stop, Oxford University for a postdoctoral fellowship.
🇫🇷🇨🇦🇬🇧
January 9, 2026 at 2:56 AM
Congratulations to the new Doctor of Philosophy @courbongautier.bsky.social on a spectacular PhD defense following a groundbreaking PhD!
Thanks to external examiner Damian Ekiert from JHU and the rest of the examining committee.
Next stop, Oxford University for a postdoctoral fellowship.
🇫🇷🇨🇦🇬🇧
Thanks to external examiner Damian Ekiert from JHU and the rest of the examining committee.
Next stop, Oxford University for a postdoctoral fellowship.
🇫🇷🇨🇦🇬🇧
Reposted by John Rubinstein
THREAD
The first full year of tracking research on @bsky.app
Hi, we are Altmetric, and we track how research is communicated across the web.
We now have one full calendar year of Bluesky research data and thought we'd have a looksie.
The first full year of tracking research on @bsky.app
Hi, we are Altmetric, and we track how research is communicated across the web.
We now have one full calendar year of Bluesky research data and thought we'd have a looksie.
January 8, 2026 at 1:08 PM
THREAD
The first full year of tracking research on @bsky.app
Hi, we are Altmetric, and we track how research is communicated across the web.
We now have one full calendar year of Bluesky research data and thought we'd have a looksie.
The first full year of tracking research on @bsky.app
Hi, we are Altmetric, and we track how research is communicated across the web.
We now have one full calendar year of Bluesky research data and thought we'd have a looksie.