John Rubinstein
@johnrubinstein.bsky.social
CryoEM, Bioenergetics, V-ATPases (especially in synaptic vesicles and lysosomes), OxPhos (especially in mycobacteria as a TB drug target)
Scientist, The Hospital for Sick Children
Professor, U of Toronto
👨👩👧🔬🚲
rubinsteinlab.org
COI: structura.bio
Scientist, The Hospital for Sick Children
Professor, U of Toronto
👨👩👧🔬🚲
rubinsteinlab.org
COI: structura.bio
Pinned
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
Reposted by John Rubinstein
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
October 30, 2025 at 3:21 AM
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
October 30, 2025 at 3:21 AM
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
I was a PhD student at LMB at the time and remember it well. Ribosome breakthroughs every few months. New students and postdocs with Venki would join his lab and have their structures published on the cover of Nature or Science within the year.
It was really freakin' annoying!
🥰
It was really freakin' annoying!
🥰
25 years on from publishing the structure of the bacterial 30S ribosomal subunit, alumni, collaborators & friends of Venki Ramakrishnan’s group joined us for a symposium celebrating the legacy of this work.
Read about the event and find photos here: www2.mrc-lmb.cam.ac.uk/25-years-of-...
#LMBNews
Read about the event and find photos here: www2.mrc-lmb.cam.ac.uk/25-years-of-...
#LMBNews
October 29, 2025 at 1:03 PM
I was a PhD student at LMB at the time and remember it well. Ribosome breakthroughs every few months. New students and postdocs with Venki would join his lab and have their structures published on the cover of Nature or Science within the year.
It was really freakin' annoying!
🥰
It was really freakin' annoying!
🥰
Reposted by John Rubinstein
Fantastic opportunity at The Hospital for Sick Children. Please repost. www.nature.com/naturecareer...
SCIENTIST – Developmental, Stem Cell & Cancer Biology Program - Toronto (City), Ontario (CA) job with The Hospital for Sick Children - Developmental & Stem Cell Biology Program | 12848200
Discovery-based and/or translational research using model organism genetics and/or stem cell and organoid platforms to study paediatric cancer.
www.nature.com
October 28, 2025 at 10:06 PM
Fantastic opportunity at The Hospital for Sick Children. Please repost. www.nature.com/naturecareer...
Reposted by John Rubinstein
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
Endogenous structure of antimalarial target PfATP4 reveals an apicomplexan-specific P-type ATPase modulator
Nature Communications - Here, the authors present the 3.7 Å cryoEM structure of native sodium efflux pump PfATP4 from Plasmodium falciparum, revealing a bound protein that they term...
rdcu.be
October 20, 2025 at 12:55 PM
Learn how our hunt for the native structure of top antimalarial target PfATP4🧂led to the discovery of PfABP, an unknown essential binding partner, out now! @natcomms.nature.com 👉 rdcu.be/eLRlH 🦠🔬❄️
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
A team effort led by @mehsehret.bsky.social & Anurag Shukla @akhilvaidya.bsky.social! #cryoEM #malaria
Reposted by John Rubinstein
🚀 Introducing fully automated data processing for repeat-target #cryoEM.
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
October 20, 2025 at 2:32 PM
🚀 Introducing fully automated data processing for repeat-target #cryoEM.
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.
Preprint: www.biorxiv.org/content/10.1...
Reposted by John Rubinstein
End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC https://www.biorxiv.org/content/10.1101/2025.10.17.682689v1
October 20, 2025 at 1:47 PM
End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC https://www.biorxiv.org/content/10.1101/2025.10.17.682689v1
Reposted by John Rubinstein
Nominations for the June Almeida award for Mid/Senior-Career Women in Cryo-EM are now open! Submit your nomination via this link www.biophysics.org/awards-fundi... by November 14th to recognize an exceptional woman in our community.
Subgroup Awards - The Biophysical Society
www.biophysics.org
October 16, 2025 at 7:08 PM
Nominations for the June Almeida award for Mid/Senior-Career Women in Cryo-EM are now open! Submit your nomination via this link www.biophysics.org/awards-fundi... by November 14th to recognize an exceptional woman in our community.
Reposted by John Rubinstein
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Structural basis of T-loop–independent recognition and activation of CDKs by the CDK-activating kinase
Cyclin-dependent kinases (CDKs) are prototypical regulators of the cell cycle. The CDK-activating kinase (CAK) acts as a master regulator of CDK activity by catalyzing the activating phosphorylation o...
www.science.org
October 16, 2025 at 7:17 PM
Now out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by John Rubinstein
9 days to go:
* 2 x open positions!
* Exceptional environment, competitive start-up package
* University of Toronto appointments
* Toronto has great (and peaceful) quality of life!
* 2 x open positions!
* Exceptional environment, competitive start-up package
* University of Toronto appointments
* Toronto has great (and peaceful) quality of life!
JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.
www.nature.com/naturecareer...
Please repost!
www.nature.com/naturecareer...
Please repost!
October 11, 2025 at 12:40 PM
9 days to go:
* 2 x open positions!
* Exceptional environment, competitive start-up package
* University of Toronto appointments
* Toronto has great (and peaceful) quality of life!
* 2 x open positions!
* Exceptional environment, competitive start-up package
* University of Toronto appointments
* Toronto has great (and peaceful) quality of life!
Reposted by John Rubinstein
Come join us at @westernu.ca: Chair, Department of Medical Biophysics: www.uwo.ca/facultyrelat...
www.uwo.ca
October 10, 2025 at 2:56 AM
Come join us at @westernu.ca: Chair, Department of Medical Biophysics: www.uwo.ca/facultyrelat...
Reposted by John Rubinstein
I'm excited to report that a species has been named after my son Ze Frank, "in recognition of his outstanding contributions to science communication, which have inspired curiosity, wonder, and deeper public engagement with the natural world"
October 6, 2025 at 7:00 PM
I'm excited to report that a species has been named after my son Ze Frank, "in recognition of his outstanding contributions to science communication, which have inspired curiosity, wonder, and deeper public engagement with the natural world"
Reposted by John Rubinstein
Our cryo-EM structure of the intermediate state of the KCNQ1 potassium channel is out. Collaborative effort with the David Fedida and Luca Maragliano labs. Cryo-EM work spearheaded by Efthimios Kyriakis, PhD
www.nature.com/articles/s41...
www.nature.com/articles/s41...
October 3, 2025 at 6:44 PM
Our cryo-EM structure of the intermediate state of the KCNQ1 potassium channel is out. Collaborative effort with the David Fedida and Luca Maragliano labs. Cryo-EM work spearheaded by Efthimios Kyriakis, PhD
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by John Rubinstein
EXCITING NEWS!! We're searching for a new professor colleague at the Biozentrum🇨🇭. Ideal candidates are exploring novel biological questions with structure, biophysics, or high-resolution imaging. 🧪 🧶🧬 🔬
The call closes Nov 3. Please get in touch if you would like more information. Come join us! 👩🔬🤓
The call closes Nov 3. Please get in touch if you would like more information. Come join us! 👩🔬🤓
September 30, 2025 at 9:52 AM
EXCITING NEWS!! We're searching for a new professor colleague at the Biozentrum🇨🇭. Ideal candidates are exploring novel biological questions with structure, biophysics, or high-resolution imaging. 🧪 🧶🧬 🔬
The call closes Nov 3. Please get in touch if you would like more information. Come join us! 👩🔬🤓
The call closes Nov 3. Please get in touch if you would like more information. Come join us! 👩🔬🤓
Reposted by John Rubinstein
🚨🧠🔬 A major breakthrough in molecular neuroscience:
I am excited to share a new story from our lab, published in accelerated format today by @nature.com:
"Delta-type glutamate receptors are ligand-gated ion channels"
Read more here (free article link): rdcu.be/eGIKz
I am excited to share a new story from our lab, published in accelerated format today by @nature.com:
"Delta-type glutamate receptors are ligand-gated ion channels"
Read more here (free article link): rdcu.be/eGIKz
September 16, 2025 at 4:47 PM
🚨🧠🔬 A major breakthrough in molecular neuroscience:
I am excited to share a new story from our lab, published in accelerated format today by @nature.com:
"Delta-type glutamate receptors are ligand-gated ion channels"
Read more here (free article link): rdcu.be/eGIKz
I am excited to share a new story from our lab, published in accelerated format today by @nature.com:
"Delta-type glutamate receptors are ligand-gated ion channels"
Read more here (free article link): rdcu.be/eGIKz
Reposted by John Rubinstein
Three Associate Professor (or Professor) positions available at Oxford Biology. Come be my colleague! www.biology.ox.ac.uk/article/we-a...
We are hiring! Oxford biology has three associate professorships available as we move to new state-of-the-art facilities
www.biology.ox.ac.uk
September 15, 2025 at 11:22 AM
Three Associate Professor (or Professor) positions available at Oxford Biology. Come be my colleague! www.biology.ox.ac.uk/article/we-a...
Reposted by John Rubinstein
Proud to be a scientist at SickKids. www.ctvnews.ca/toronto/arti... @sickkidsto.bsky.social
SickKids named world’s best pediatric hospital in new ranking
The Hospital for Sick Children in Toronto has topped a new list ranking the world’s top pediatric hospitals.
www.ctvnews.ca
September 12, 2025 at 12:49 PM
Proud to be a scientist at SickKids. www.ctvnews.ca/toronto/arti... @sickkidsto.bsky.social
Reposted by John Rubinstein
First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...
Leopard-EM: An extensible 2DTM package to accelerate in situ structural biology
The ability to generate high-resolution views of cells with cryogenic electron microscopy (cryo-EM) can reveal the molecular mechanisms of biological processes in their native cellular context. The re...
www.biorxiv.org
August 30, 2025 at 12:49 AM
First pre-print from the Lucas Lab led by Matthew Giammar and @joshdcryoem.bsky.social online today! We develop a new, extensible Python implementation of 2DTM and apply it to build pixel size optimization, constrained search and characterize molecular motions in situ www.biorxiv.org/content/10.1...
Reposted by John Rubinstein
JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.
www.nature.com/naturecareer...
Please repost!
www.nature.com/naturecareer...
Please repost!
August 28, 2025 at 6:34 AM
JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.
www.nature.com/naturecareer...
Please repost!
www.nature.com/naturecareer...
Please repost!
Reposted by John Rubinstein
I mentioned to a couple of people that I had a surprise waiting to be shared when Moderna got approval for its updated COVID-19 vaccine. It’s approved, and the big news??? Completely produced domestically!!!!! 🥳🤩
Approved as usual for all aged 6mo+!
Approved as usual for all aged 6mo+!
August 23, 2025 at 2:12 PM
I mentioned to a couple of people that I had a surprise waiting to be shared when Moderna got approval for its updated COVID-19 vaccine. It’s approved, and the big news??? Completely produced domestically!!!!! 🥳🤩
Approved as usual for all aged 6mo+!
Approved as usual for all aged 6mo+!
Montréal keeps getting better and better!
The city of Montréal has just announced that Ste Catherine Street will become permanently pedestrianized next year, as part of a beautification project of the Village including leafy trees, softer walkways and comfy installations. We’re jumping for joy! 🤩
📷 @evablue.bsky.social
#montreal
📷 @evablue.bsky.social
#montreal
August 23, 2025 at 4:53 PM
Montréal keeps getting better and better!
Reposted by John Rubinstein
Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug
Nature Structural & Molecular Biology - Integrated structural biology approach leveraging in situ cryo-electron tomography reveals molecular details of the native malarial translation...
rdcu.be
August 18, 2025 at 12:13 PM
Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo
Reposted by John Rubinstein
I can save Carney heaps—start with NFRF, CERC & the global innovation clusters.
DON’T touch tricouncil funding.
This would be the worst ROI & catastrophic for 🇨🇦’s research, innovation, training & economic development & growth.
@liberalca.bsky.social
@mark-carney.bsky.social
DON’T touch tricouncil funding.
This would be the worst ROI & catastrophic for 🇨🇦’s research, innovation, training & economic development & growth.
@liberalca.bsky.social
@mark-carney.bsky.social
Canada plans a 15% budget cut. Scientists are alarmed
Cuts could erase promised boost, researchers fear
www.science.org
August 9, 2025 at 10:29 PM
I can save Carney heaps—start with NFRF, CERC & the global innovation clusters.
DON’T touch tricouncil funding.
This would be the worst ROI & catastrophic for 🇨🇦’s research, innovation, training & economic development & growth.
@liberalca.bsky.social
@mark-carney.bsky.social
DON’T touch tricouncil funding.
This would be the worst ROI & catastrophic for 🇨🇦’s research, innovation, training & economic development & growth.
@liberalca.bsky.social
@mark-carney.bsky.social
Whoever designed the new landscaping around King's College Circle at @utoronto.ca has done a brilliant job!
All summer I've been getting off the subway early to walk down Philosopher's Walk and the new walkways on the St. George campus - a great half hour start to each day.
All summer I've been getting off the subway early to walk down Philosopher's Walk and the new walkways on the St. George campus - a great half hour start to each day.
August 15, 2025 at 5:36 PM
Whoever designed the new landscaping around King's College Circle at @utoronto.ca has done a brilliant job!
All summer I've been getting off the subway early to walk down Philosopher's Walk and the new walkways on the St. George campus - a great half hour start to each day.
All summer I've been getting off the subway early to walk down Philosopher's Walk and the new walkways on the St. George campus - a great half hour start to each day.