Fillip Port
@crisprflydesign.bsky.social
Scientist at the German Cancer Reseach Center in Heidelberg. We develop genome engineering tools for functional genomics. #CRISPR #Drosophila #openscience
Pinned
Our new preprint is out - please share!
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
Reposted by Fillip Port
We are recruiting a tenure track professor in the broader area of molecular biology @zmbh.uni-heidelberg.de at Heidelberg University! www.nature.com/naturecareer... Please share and apply!
Tenure Track Professorship (W1 with Tenure Track to W3) in “Molecular Biology" (f/m/d) - Heidelberg job with Universität Heidelberg | 12850668
The Center for Molecular Biology of Heidelberg University (ZMBH) and the Faculty of Biosciences invite applications for a Tenure Track Professors...
www.nature.com
December 9, 2025 at 6:11 PM
We are recruiting a tenure track professor in the broader area of molecular biology @zmbh.uni-heidelberg.de at Heidelberg University! www.nature.com/naturecareer... Please share and apply!
Reposted by Fillip Port
“A program with a 0.5% success rate is not elite. It’s wasteful. Thousands of highly trained scientists will sink dozens of hours into writing proposals with vanishing odds of success. This is not just inefficient — it’s disrespectful of scientific labour.” Very well said @kamounlab.bsky.social!👏🏼👏🏼👏🏼
I just published: ERC-Plus: jackpot science or missed chance to fix academia?
My reflections on ERC-Plus, Europe’s newest ultra-competitive research grant and what it tells us about the academic culture we’re building.
medium.com/p/erc-plus-j...
My reflections on ERC-Plus, Europe’s newest ultra-competitive research grant and what it tells us about the academic culture we’re building.
medium.com/p/erc-plus-j...
ERC-Plus: jackpot science or missed chance to fix academia?
My reflections on ERC-Plus, Europe’s newest ultra-competitive research grant and what it tells us about the academic culture we’re…
medium.com
December 3, 2025 at 10:15 PM
“A program with a 0.5% success rate is not elite. It’s wasteful. Thousands of highly trained scientists will sink dozens of hours into writing proposals with vanishing odds of success. This is not just inefficient — it’s disrespectful of scientific labour.” Very well said @kamounlab.bsky.social!👏🏼👏🏼👏🏼
Reposted by Fillip Port
Reposted by Fillip Port
Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
December 2, 2025 at 9:02 PM
Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
Reposted by Fillip Port
The hidden danger of Biorender
(& the death of scientific illustration)
A short thread 🧵
(& the death of scientific illustration)
A short thread 🧵
December 1, 2025 at 12:52 PM
The hidden danger of Biorender
(& the death of scientific illustration)
A short thread 🧵
(& the death of scientific illustration)
A short thread 🧵
Reposted by Fillip Port
Es stehen erscheinende Tage für eine moderne, wissenschaftsbasierte Regulierung von Neuen Genomischen Techniken wie #CRISPR & Co. in der EU an.
Worum es geht und was damit zu erreichen ist, haben wir 👇 nochmal zusammengefasst.
#GiveGenesAChance
Worum es geht und was damit zu erreichen ist, haben wir 👇 nochmal zusammengefasst.
#GiveGenesAChance
🇪🇺 Warum sich eine moderne, sachgerechte Regulierung von NGT lohnt 🌱🧬
Die Neuen Genomischen Techniken (NGT) bieten ein echtes Potenzial für eine nachhaltige, klimaresiliente & effiziente Landwirtschaft. Wissenschaftliche Studien liefern keine belastbaren Hinweise auf Gefahren, während gleichzeitig/
Die Neuen Genomischen Techniken (NGT) bieten ein echtes Potenzial für eine nachhaltige, klimaresiliente & effiziente Landwirtschaft. Wissenschaftliche Studien liefern keine belastbaren Hinweise auf Gefahren, während gleichzeitig/
November 26, 2025 at 8:47 AM
Es stehen erscheinende Tage für eine moderne, wissenschaftsbasierte Regulierung von Neuen Genomischen Techniken wie #CRISPR & Co. in der EU an.
Worum es geht und was damit zu erreichen ist, haben wir 👇 nochmal zusammengefasst.
#GiveGenesAChance
Worum es geht und was damit zu erreichen ist, haben wir 👇 nochmal zusammengefasst.
#GiveGenesAChance
Reposted by Fillip Port
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
November 24, 2025 at 4:57 PM
#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨🔬 Built by a researcher — for researchers!
👉 Check it out at opencloning.org
Reposted by Fillip Port
Worked in a Drosophila genetics lab for the sole purpose of getting a letter of recommendation from the lab head for medical school. I loved being in the lab so much that I gave up on medical school and went to graduate school instead.
November 23, 2025 at 2:28 PM
Worked in a Drosophila genetics lab for the sole purpose of getting a letter of recommendation from the lab head for medical school. I loved being in the lab so much that I gave up on medical school and went to graduate school instead.
Reposted by Fillip Port
Reposted by Fillip Port
1/9 Metagenomics lets us read microbiomes in nature without cultivation, but writing (editing) them in their native context is still a major challenge.
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
science.org
November 14, 2025 at 2:25 PM
1/9 Metagenomics lets us read microbiomes in nature without cultivation, but writing (editing) them in their native context is still a major challenge.
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Reposted by Fillip Port
![[Scene is a kitchen - a middle aged woman called JANET is boiling peas at the stove. A younger more colourfully dressed woman named LIZ approached her.]
JANET:
Ugh...
LIZ:
What's up?
JANET:
I am so bored of cooking peas!
LIZ:
Have you tried...
AI peas?
JANET:
AI peas?
LIZ:
They're peas with AI!
[Liz holds up to us a packet of peas labelled: Pea-i AI - Peas with AI].
LIZ:
Al-powered peas harness the potential of your peas
JANET:
What
LIZ [Now a voiceover as we cut to a whizzy technology diagram of peas all connected by meaningless dotted lines]
Why not take your peas to the next level with Al Peas' new Al tools to power your peas?
[Show a techno diagram of a pea with a label reading 'AI' pointing to a random zone in it]
LIZ:
Each pea has Al in a way we haven't quite worked out yet but it's fine
[Show Janet and Liz now in a Matrix-style world of peas]
LIZ:
With Al peas you can supercharge productivity and make AI work for your peas!
JANET:
What
LIZ:
Shut up
LIZ:
Our game-changing Pea-Al gives you the freedom to unlock the potential of the power of the future of your peas workflow
From opening the bag of peas
to boiling the peas
to eating the peas
To spending millions on adding Al to the peas and then having to work out what that even means.
JANET:
Is it really necessary to-
LIZ [Grabbing Janet by the collar]:
THE PEAS HAVE GOT AI, JANET
[Cut to an advert ending screen, with the bag of peas and the slogan:
AI PEAS: Just 'Peas' for god's sake buy the AI peas.
[Ends]](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:4m3we4apbjayclzcnhwe3pfp/bafkreiayqa37f7kik7l7s7fbtfp2sir5ckb662ry2lylinyjrfa2rkvvka@jpeg)
Reposted by Fillip Port
Excited to share our new paper out in Cell Reports Methods! @cp-cellrepmethods.bsky.social
🎉🎉🎉
We present EyaHOST, a modular Drosophila genetic system that enables precise and independent manipulation of tumours and host tissues to dissect tumour-host interactions.
www.cell.com/cell-reports...
🎉🎉🎉
We present EyaHOST, a modular Drosophila genetic system that enables precise and independent manipulation of tumours and host tissues to dissect tumour-host interactions.
www.cell.com/cell-reports...
EyaHOST, a modular genetic system for investigation of intercellular and tumor-host interactions in Drosophila melanogaster
Teles-Reis et al. present EyaHOST, a modular Drosophila system that enables clone
generation in the eye disc with independent genetic manipulation of other tissues.
EyaHOST uses an Eya-KD “kick-out” s...
www.cell.com
November 5, 2025 at 11:07 PM
Excited to share our new paper out in Cell Reports Methods! @cp-cellrepmethods.bsky.social
🎉🎉🎉
We present EyaHOST, a modular Drosophila genetic system that enables precise and independent manipulation of tumours and host tissues to dissect tumour-host interactions.
www.cell.com/cell-reports...
🎉🎉🎉
We present EyaHOST, a modular Drosophila genetic system that enables precise and independent manipulation of tumours and host tissues to dissect tumour-host interactions.
www.cell.com/cell-reports...
Reposted by Fillip Port
Funders can drive change. Authors can't. Authors are producers, not consumers. Producers don't decide demand, consumers do. Funders and research institutes must demand change of their authors, or withhold their funds. It's that simple. Funders have all the cards.
10/n
10/n
November 11, 2025 at 11:52 AM
Funders can drive change. Authors can't. Authors are producers, not consumers. Producers don't decide demand, consumers do. Funders and research institutes must demand change of their authors, or withhold their funds. It's that simple. Funders have all the cards.
10/n
10/n
Reposted by Fillip Port
That's why funders hold the power here. Funders can decide to pay for services or not. They can decide to support authors who publish in for-profit journals, or not. They can decide to pay APCs in for-profit journals, or not.
9/n
9/n
November 11, 2025 at 11:52 AM
That's why funders hold the power here. Funders can decide to pay for services or not. They can decide to support authors who publish in for-profit journals, or not. They can decide to pay APCs in for-profit journals, or not.
9/n
9/n
Reposted by Fillip Port
Back on track:
Funders hold all the cards. There's this flawed view that authors are consumers and journals are producers. Wrong.
Funders and institutes are consumers. They contract authors to produce research, and they pay for journals to QC the work.
Funders are the consumers.
8/n
Funders hold all the cards. There's this flawed view that authors are consumers and journals are producers. Wrong.
Funders and institutes are consumers. They contract authors to produce research, and they pay for journals to QC the work.
Funders are the consumers.
8/n
November 11, 2025 at 11:52 AM
Back on track:
Funders hold all the cards. There's this flawed view that authors are consumers and journals are producers. Wrong.
Funders and institutes are consumers. They contract authors to produce research, and they pay for journals to QC the work.
Funders are the consumers.
8/n
Funders hold all the cards. There's this flawed view that authors are consumers and journals are producers. Wrong.
Funders and institutes are consumers. They contract authors to produce research, and they pay for journals to QC the work.
Funders are the consumers.
8/n
Reposted by Fillip Port
This October I’m drawing 1 molecule a day inspired by proteins in pdb @rcsbpdb.bsky.social
Day 23/31
Prompt FIREFLY
Pdb: 2D1R
Luciferase: firefly enzyme catalyzes a light-producing reaction that makes fireflies glow.
Next: ROWDY
Suggestions?
Day 23/31
Prompt FIREFLY
Pdb: 2D1R
Luciferase: firefly enzyme catalyzes a light-producing reaction that makes fireflies glow.
Next: ROWDY
Suggestions?
November 7, 2025 at 3:22 AM
This October I’m drawing 1 molecule a day inspired by proteins in pdb @rcsbpdb.bsky.social
Day 23/31
Prompt FIREFLY
Pdb: 2D1R
Luciferase: firefly enzyme catalyzes a light-producing reaction that makes fireflies glow.
Next: ROWDY
Suggestions?
Day 23/31
Prompt FIREFLY
Pdb: 2D1R
Luciferase: firefly enzyme catalyzes a light-producing reaction that makes fireflies glow.
Next: ROWDY
Suggestions?
Reposted by Fillip Port
Delighted to share Array Assembler — a web tool for designing oligos for generating crRNA arrays for #Cas12 and #Cas13 systems. Input spacers and key plasmid details to generate an order-ready XLSX of oligos, streamlining array creation while minimising cost, time, and errors. Happy CRISPRing!
May 8, 2025 at 7:59 PM
Reposted by Fillip Port
Lots of exciting PhD projects on offer at the LMB, including in our group 😊
Considering the LMB for your PhD?
We’ve got over 25 projects available, covering a wide array of topics. Check them out here: www2.mrc-lmb.cam.ac.uk/students/int...
Apply by 2nd DEC for Autumn 2026 admission.
We’ve got over 25 projects available, covering a wide array of topics. Check them out here: www2.mrc-lmb.cam.ac.uk/students/int...
Apply by 2nd DEC for Autumn 2026 admission.
November 10, 2025 at 2:30 PM
Lots of exciting PhD projects on offer at the LMB, including in our group 😊
Reposted by Fillip Port
Our lab is actively looking for PhD students and there is NOW an Open Call for PhD fellowship at the IRB Barcelona!!!!! phd.irbbarcelona.org
Application Guidelines - IRB PhD Call 2026 NOW OPEN! | Irb
phd.irbbarcelona.org
November 7, 2025 at 3:21 PM
Our lab is actively looking for PhD students and there is NOW an Open Call for PhD fellowship at the IRB Barcelona!!!!! phd.irbbarcelona.org
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 6, 2025 at 5:20 PM
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Fillip Port
Our latest paper has just been published in Cell!
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
November 5, 2025 at 5:20 PM
Our latest paper has just been published in Cell!
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
Reposted by Fillip Port
🧬Proud to share "Improved specificity and efficiency of in vivo adenine base editing therapies with hybrid guide RNAs" my 1st first-author paper of my PhD, co-first-authored with the brilliant Dr. @mnwhittaker.bsky.social, is out today in Nature Biomedical Engineering! www.nature.com/articles/s41...
Improved specificity and efficiency of in vivo adenine base editing therapies with hybrid guide RNAs - Nature Biomedical Engineering
Hybrid gRNAs significantly increase targeted editing in the liver while simultaneously reducing unwanted bystander editing in humanized mouse models.
www.nature.com
October 28, 2025 at 12:58 PM
🧬Proud to share "Improved specificity and efficiency of in vivo adenine base editing therapies with hybrid guide RNAs" my 1st first-author paper of my PhD, co-first-authored with the brilliant Dr. @mnwhittaker.bsky.social, is out today in Nature Biomedical Engineering! www.nature.com/articles/s41...
Reposted by Fillip Port
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Cell-stereotyped DNA repair outcomes are widespread during genome editing
Genome editing outcomes are governed by DNA repair pathways that vary with cell type and state. We developed scOUT-seq (single-cell Outcomes Using Transcript sequencing), a scalable approach that join...
www.biorxiv.org
October 24, 2025 at 8:44 AM
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Looking forward to visiting @irbbarcelona.org and @milanlab.bsky.social on Friday. Will talk about our work updating the toolbox for engineering the fly genome. Come by if you are in the area. @araujolabub.bsky.social
@gonzalezlabbcn.bsky.social
@mirimiam.bsky.social
@monicabettencourt.bsky.social
@gonzalezlabbcn.bsky.social
@mirimiam.bsky.social
@monicabettencourt.bsky.social
Join us in our plenary #seminar this week, with @crisprflydesign.bsky.social, from @dkfz.bsky.social.
Host: Dr. @milanlab.bsky.social
Register here ➡️ https://shorturl.at/gBv1J
#Drosophila #DrosophilaResearch #CRISPR
🧪
Host: Dr. @milanlab.bsky.social
Register here ➡️ https://shorturl.at/gBv1J
#Drosophila #DrosophilaResearch #CRISPR
🧪
October 14, 2025 at 7:09 AM
Looking forward to visiting @irbbarcelona.org and @milanlab.bsky.social on Friday. Will talk about our work updating the toolbox for engineering the fly genome. Come by if you are in the area. @araujolabub.bsky.social
@gonzalezlabbcn.bsky.social
@mirimiam.bsky.social
@monicabettencourt.bsky.social
@gonzalezlabbcn.bsky.social
@mirimiam.bsky.social
@monicabettencourt.bsky.social
Reposted by Fillip Port
Love RNA biology?
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
PIWI clade Argonautes are essential for transposon silencing. Without them, animals are sterile due to massive transposon activity.
But how does piRNA-guided target interaction translate into silencing?
PhD student Júlia Portell Montserrat has an intriguing answer
www.cell.com/molecular-ce...
But how does piRNA-guided target interaction translate into silencing?
PhD student Júlia Portell Montserrat has an intriguing answer
www.cell.com/molecular-ce...
September 18, 2025 at 2:34 PM
Love RNA biology?
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social