Fillip Port
@crisprflydesign.bsky.social
Scientist at the German Cancer Reseach Center in Heidelberg. We develop genome engineering tools for functional genomics. #CRISPR #Drosophila #openscience
Pinned
Our new preprint is out - please share!
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
We asked: How can we make CRISPR knockouts in vivo more efficient? And set up an assay that enables detection of CRISPR cutting with 100s-1000s of sgRNAs over entire chromosome arms in living animals.
Grab a ☕ and let’s dive in. 🧵🧪 1/n
#CRISPR #geneediting
Reposted by Fillip Port
1/9 Metagenomics lets us read microbiomes in nature without cultivation, but writing (editing) them in their native context is still a major challenge.
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Although metagenomic sequencing has revealed a rich microbial biodiversity in the mammalian gut, methods to genetically alter specific species in the microbiome are highly limited. Here, we introduce ...
science.org
November 14, 2025 at 2:25 PM
1/9 Metagenomics lets us read microbiomes in nature without cultivation, but writing (editing) them in their native context is still a major challenge.
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Meet MetaEdit: a platform for pathway-scale metagenomic editing inside the gut microbiome. science.org/doi/10.1126/...
Reposted by Fillip Port
![[Scene is a kitchen - a middle aged woman called JANET is boiling peas at the stove. A younger more colourfully dressed woman named LIZ approached her.]
JANET:
Ugh...
LIZ:
What's up?
JANET:
I am so bored of cooking peas!
LIZ:
Have you tried...
AI peas?
JANET:
AI peas?
LIZ:
They're peas with AI!
[Liz holds up to us a packet of peas labelled: Pea-i AI - Peas with AI].
LIZ:
Al-powered peas harness the potential of your peas
JANET:
What
LIZ [Now a voiceover as we cut to a whizzy technology diagram of peas all connected by meaningless dotted lines]
Why not take your peas to the next level with Al Peas' new Al tools to power your peas?
[Show a techno diagram of a pea with a label reading 'AI' pointing to a random zone in it]
LIZ:
Each pea has Al in a way we haven't quite worked out yet but it's fine
[Show Janet and Liz now in a Matrix-style world of peas]
LIZ:
With Al peas you can supercharge productivity and make AI work for your peas!
JANET:
What
LIZ:
Shut up
LIZ:
Our game-changing Pea-Al gives you the freedom to unlock the potential of the power of the future of your peas workflow
From opening the bag of peas
to boiling the peas
to eating the peas
To spending millions on adding Al to the peas and then having to work out what that even means.
JANET:
Is it really necessary to-
LIZ [Grabbing Janet by the collar]:
THE PEAS HAVE GOT AI, JANET
[Cut to an advert ending screen, with the bag of peas and the slogan:
AI PEAS: Just 'Peas' for god's sake buy the AI peas.
[Ends]](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:4m3we4apbjayclzcnhwe3pfp/bafkreiayqa37f7kik7l7s7fbtfp2sir5ckb662ry2lylinyjrfa2rkvvka@jpeg)
Reposted by Fillip Port
Excited to share our new paper out in Cell Reports Methods! @cp-cellrepmethods.bsky.social
🎉🎉🎉
We present EyaHOST, a modular Drosophila genetic system that enables precise and independent manipulation of tumours and host tissues to dissect tumour-host interactions.
www.cell.com/cell-reports...
🎉🎉🎉
We present EyaHOST, a modular Drosophila genetic system that enables precise and independent manipulation of tumours and host tissues to dissect tumour-host interactions.
www.cell.com/cell-reports...
EyaHOST, a modular genetic system for investigation of intercellular and tumor-host interactions in Drosophila melanogaster
Teles-Reis et al. present EyaHOST, a modular Drosophila system that enables clone
generation in the eye disc with independent genetic manipulation of other tissues.
EyaHOST uses an Eya-KD “kick-out” s...
www.cell.com
November 5, 2025 at 11:07 PM
Excited to share our new paper out in Cell Reports Methods! @cp-cellrepmethods.bsky.social
🎉🎉🎉
We present EyaHOST, a modular Drosophila genetic system that enables precise and independent manipulation of tumours and host tissues to dissect tumour-host interactions.
www.cell.com/cell-reports...
🎉🎉🎉
We present EyaHOST, a modular Drosophila genetic system that enables precise and independent manipulation of tumours and host tissues to dissect tumour-host interactions.
www.cell.com/cell-reports...
Reposted by Fillip Port
Funders can drive change. Authors can't. Authors are producers, not consumers. Producers don't decide demand, consumers do. Funders and research institutes must demand change of their authors, or withhold their funds. It's that simple. Funders have all the cards.
10/n
10/n
November 11, 2025 at 11:52 AM
Funders can drive change. Authors can't. Authors are producers, not consumers. Producers don't decide demand, consumers do. Funders and research institutes must demand change of their authors, or withhold their funds. It's that simple. Funders have all the cards.
10/n
10/n
Reposted by Fillip Port
That's why funders hold the power here. Funders can decide to pay for services or not. They can decide to support authors who publish in for-profit journals, or not. They can decide to pay APCs in for-profit journals, or not.
9/n
9/n
November 11, 2025 at 11:52 AM
That's why funders hold the power here. Funders can decide to pay for services or not. They can decide to support authors who publish in for-profit journals, or not. They can decide to pay APCs in for-profit journals, or not.
9/n
9/n
Reposted by Fillip Port
Back on track:
Funders hold all the cards. There's this flawed view that authors are consumers and journals are producers. Wrong.
Funders and institutes are consumers. They contract authors to produce research, and they pay for journals to QC the work.
Funders are the consumers.
8/n
Funders hold all the cards. There's this flawed view that authors are consumers and journals are producers. Wrong.
Funders and institutes are consumers. They contract authors to produce research, and they pay for journals to QC the work.
Funders are the consumers.
8/n
November 11, 2025 at 11:52 AM
Back on track:
Funders hold all the cards. There's this flawed view that authors are consumers and journals are producers. Wrong.
Funders and institutes are consumers. They contract authors to produce research, and they pay for journals to QC the work.
Funders are the consumers.
8/n
Funders hold all the cards. There's this flawed view that authors are consumers and journals are producers. Wrong.
Funders and institutes are consumers. They contract authors to produce research, and they pay for journals to QC the work.
Funders are the consumers.
8/n
Reposted by Fillip Port
This October I’m drawing 1 molecule a day inspired by proteins in pdb @rcsbpdb.bsky.social
Day 23/31
Prompt FIREFLY
Pdb: 2D1R
Luciferase: firefly enzyme catalyzes a light-producing reaction that makes fireflies glow.
Next: ROWDY
Suggestions?
Day 23/31
Prompt FIREFLY
Pdb: 2D1R
Luciferase: firefly enzyme catalyzes a light-producing reaction that makes fireflies glow.
Next: ROWDY
Suggestions?
November 7, 2025 at 3:22 AM
This October I’m drawing 1 molecule a day inspired by proteins in pdb @rcsbpdb.bsky.social
Day 23/31
Prompt FIREFLY
Pdb: 2D1R
Luciferase: firefly enzyme catalyzes a light-producing reaction that makes fireflies glow.
Next: ROWDY
Suggestions?
Day 23/31
Prompt FIREFLY
Pdb: 2D1R
Luciferase: firefly enzyme catalyzes a light-producing reaction that makes fireflies glow.
Next: ROWDY
Suggestions?
Reposted by Fillip Port
Delighted to share Array Assembler — a web tool for designing oligos for generating crRNA arrays for #Cas12 and #Cas13 systems. Input spacers and key plasmid details to generate an order-ready XLSX of oligos, streamlining array creation while minimising cost, time, and errors. Happy CRISPRing!
May 8, 2025 at 7:59 PM
Reposted by Fillip Port
Lots of exciting PhD projects on offer at the LMB, including in our group 😊
Considering the LMB for your PhD?
We’ve got over 25 projects available, covering a wide array of topics. Check them out here: www2.mrc-lmb.cam.ac.uk/students/int...
Apply by 2nd DEC for Autumn 2026 admission.
We’ve got over 25 projects available, covering a wide array of topics. Check them out here: www2.mrc-lmb.cam.ac.uk/students/int...
Apply by 2nd DEC for Autumn 2026 admission.
November 10, 2025 at 2:30 PM
Lots of exciting PhD projects on offer at the LMB, including in our group 😊
Reposted by Fillip Port
Our lab is actively looking for PhD students and there is NOW an Open Call for PhD fellowship at the IRB Barcelona!!!!! phd.irbbarcelona.org
Application Guidelines - IRB PhD Call 2026 NOW OPEN! | Irb
phd.irbbarcelona.org
November 7, 2025 at 3:21 PM
Our lab is actively looking for PhD students and there is NOW an Open Call for PhD fellowship at the IRB Barcelona!!!!! phd.irbbarcelona.org
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 6, 2025 at 5:20 PM
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Fillip Port
Our latest paper has just been published in Cell!
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
November 5, 2025 at 5:20 PM
Our latest paper has just been published in Cell!
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
doi.org/10.1016/j.ce...
We developed a new method called MCC ultra, which allows 3D chromatin structure to be visualised with a 1 base pair pixel size.
Reposted by Fillip Port
🧬Proud to share "Improved specificity and efficiency of in vivo adenine base editing therapies with hybrid guide RNAs" my 1st first-author paper of my PhD, co-first-authored with the brilliant Dr. @mnwhittaker.bsky.social, is out today in Nature Biomedical Engineering! www.nature.com/articles/s41...
Improved specificity and efficiency of in vivo adenine base editing therapies with hybrid guide RNAs - Nature Biomedical Engineering
Hybrid gRNAs significantly increase targeted editing in the liver while simultaneously reducing unwanted bystander editing in humanized mouse models.
www.nature.com
October 28, 2025 at 12:58 PM
🧬Proud to share "Improved specificity and efficiency of in vivo adenine base editing therapies with hybrid guide RNAs" my 1st first-author paper of my PhD, co-first-authored with the brilliant Dr. @mnwhittaker.bsky.social, is out today in Nature Biomedical Engineering! www.nature.com/articles/s41...
Reposted by Fillip Port
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Cell-stereotyped DNA repair outcomes are widespread during genome editing
Genome editing outcomes are governed by DNA repair pathways that vary with cell type and state. We developed scOUT-seq (single-cell Outcomes Using Transcript sequencing), a scalable approach that join...
www.biorxiv.org
October 24, 2025 at 8:44 AM
Ever noticed that #CRISPR editing results differ between cells? Awesome PhD student Moritz Schlapansky developed "scOUT-seq" to measure single cell transcriptomes + editing. 1.2 million cells, 74 cell types, living 🐭. Cell subtypes differ wildly from bulk average! www.biorxiv.org/content/10.1...
Looking forward to visiting @irbbarcelona.org and @milanlab.bsky.social on Friday. Will talk about our work updating the toolbox for engineering the fly genome. Come by if you are in the area. @araujolabub.bsky.social
@gonzalezlabbcn.bsky.social
@mirimiam.bsky.social
@monicabettencourt.bsky.social
@gonzalezlabbcn.bsky.social
@mirimiam.bsky.social
@monicabettencourt.bsky.social
Join us in our plenary #seminar this week, with @crisprflydesign.bsky.social, from @dkfz.bsky.social.
Host: Dr. @milanlab.bsky.social
Register here ➡️ https://shorturl.at/gBv1J
#Drosophila #DrosophilaResearch #CRISPR
🧪
Host: Dr. @milanlab.bsky.social
Register here ➡️ https://shorturl.at/gBv1J
#Drosophila #DrosophilaResearch #CRISPR
🧪
October 14, 2025 at 7:09 AM
Looking forward to visiting @irbbarcelona.org and @milanlab.bsky.social on Friday. Will talk about our work updating the toolbox for engineering the fly genome. Come by if you are in the area. @araujolabub.bsky.social
@gonzalezlabbcn.bsky.social
@mirimiam.bsky.social
@monicabettencourt.bsky.social
@gonzalezlabbcn.bsky.social
@mirimiam.bsky.social
@monicabettencourt.bsky.social
Reposted by Fillip Port
Love RNA biology?
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
PIWI clade Argonautes are essential for transposon silencing. Without them, animals are sterile due to massive transposon activity.
But how does piRNA-guided target interaction translate into silencing?
PhD student Júlia Portell Montserrat has an intriguing answer
www.cell.com/molecular-ce...
But how does piRNA-guided target interaction translate into silencing?
PhD student Júlia Portell Montserrat has an intriguing answer
www.cell.com/molecular-ce...
September 18, 2025 at 2:34 PM
Love RNA biology?
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
Join us to explore the piRNA pathway with structural and genetic approaches (see 👇👇).
PhD student/postdoc position co-supervised by Clemens Plaschka & myself.
DM or email us if you’d like to know more!
@vbcscitraining.bsky.social @imbavienna.bsky.social @impvienna.bsky.social
High levels of Cas9 are toxic in sensory neurons. Reducing Cas9 levels with uORFs avoids toxicity and is compatible with efficient editing. From @thompsonpeerlab.bsky.social. Fly lines @vdrc-flies.bsky.social
#CRISPR #Drosophila
www.biorxiv.org/content/10.1...
#CRISPR #Drosophila
www.biorxiv.org/content/10.1...
October 4, 2025 at 9:10 PM
High levels of Cas9 are toxic in sensory neurons. Reducing Cas9 levels with uORFs avoids toxicity and is compatible with efficient editing. From @thompsonpeerlab.bsky.social. Fly lines @vdrc-flies.bsky.social
#CRISPR #Drosophila
www.biorxiv.org/content/10.1...
#CRISPR #Drosophila
www.biorxiv.org/content/10.1...
Reposted by Fillip Port
Macron remarks are notable- some quotes: "We have been incredibly naive in entrusting our democratic space to social networks that are controlled either by large American entrepreneurs or large Chinese companies, whose interests are not at all the survival or proper functioning of our democracies."
President Macron: “Europeans, let's wake up!
We have been incredibly naive in entrusting our democratic space to social networks.”
defenddemocracy.eu/macron-democ...
We have been incredibly naive in entrusting our democratic space to social networks.”
defenddemocracy.eu/macron-democ...
October 4, 2025 at 11:57 AM
Macron remarks are notable- some quotes: "We have been incredibly naive in entrusting our democratic space to social networks that are controlled either by large American entrepreneurs or large Chinese companies, whose interests are not at all the survival or proper functioning of our democracies."
Reposted by Fillip Port
💫NEW: @telemanlab.bsky.social & co demonstrate that #AMPK can be activated by signaling metabolite, #adenosine, under non-stress conditions during #Drosophila development. The intestine regulates adenosine levels, thus, remotely controlling wing disc AMPK activation and growth.
bit.ly/4gX6njF
bit.ly/4gX6njF
Remote control of AMPK via extracellular adenosine controls tissue growth - Nature Cell Biology
Zhang et al. demonstrate that AMPK can be activated by signalling metabolite, adenosine, under non-stress conditions during Drosophila development. The intestine regulates adenosine levels, thus, remotely controlling wing disc AMPK activation and growth.
bit.ly
October 4, 2025 at 2:32 PM
💫NEW: @telemanlab.bsky.social & co demonstrate that #AMPK can be activated by signaling metabolite, #adenosine, under non-stress conditions during #Drosophila development. The intestine regulates adenosine levels, thus, remotely controlling wing disc AMPK activation and growth.
bit.ly/4gX6njF
bit.ly/4gX6njF
Reposted by Fillip Port
I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
October 4, 2025 at 11:50 AM
I’m happy to share some plugins I’ve been developping this summer: "Channels and Contrast" and LUTs Manager!
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
I can’t find new bugs and ideas by now so I need your help to please test them in your machines and report bugs, feedbacks and ideas! forum.image.sc/t/looking-fo...
What an exciting opportunity! Setting up a new lab with the great @madalenamrp.bsky.social at one of the best institutes around. Apply! 🧪🐟🧬
Are you interested in a PhD in the area of "Mechanisms of translational control during embryogenesis?" Apply now for a PhD @mpi-cbg.de! Overview of all participating RGLs: imprs.mpi-cbg.de/5761/researc...
October 4, 2025 at 11:43 AM
What an exciting opportunity! Setting up a new lab with the great @madalenamrp.bsky.social at one of the best institutes around. Apply! 🧪🐟🧬
Reposted by Fillip Port
If you’re applying to your dream lab for an internship/PhD/postdoc, always send a second email 1-2 weeks after the first one if you don’t hear back.
I promise we will be grateful rather than annoyed. My email inbox is a disaster and I’m quite junior - and very few of us have secretarial support
I promise we will be grateful rather than annoyed. My email inbox is a disaster and I’m quite junior - and very few of us have secretarial support
October 4, 2025 at 5:47 AM
If you’re applying to your dream lab for an internship/PhD/postdoc, always send a second email 1-2 weeks after the first one if you don’t hear back.
I promise we will be grateful rather than annoyed. My email inbox is a disaster and I’m quite junior - and very few of us have secretarial support
I promise we will be grateful rather than annoyed. My email inbox is a disaster and I’m quite junior - and very few of us have secretarial support
Reposted by Fillip Port
You can read Mark's 2024 @dev-journal.bsky.social Review article here: doi.org/10.1242/dev....
40 years since the Heidelberg genetic screen that revolutionized developmental and cell biology
ABSTRACT. Our current understanding of the molecular basis of embryonic development and the shared machinery underlying this remarkable process has its roots in three papers published 40 years ago, wh...
doi.org
October 3, 2025 at 4:13 PM
You can read Mark's 2024 @dev-journal.bsky.social Review article here: doi.org/10.1242/dev....
Reposted by Fillip Port
My entry for today’s #FluorescenceFriday: a pupal #Drosophila testis with muscles expressing
🔵 lifeact &
🔴 RFP-nls
Honored & grateful to receive an honorable mention at @healthcare.nikon.com Nikon Small World 🌍🔬✨
🔗 www.nikonsmallworld.com/galleries/20...
#NikonSmallWorld #Microscopy #ScienceArt
🔵 lifeact &
🔴 RFP-nls
Honored & grateful to receive an honorable mention at @healthcare.nikon.com Nikon Small World 🌍🔬✨
🔗 www.nikonsmallworld.com/galleries/20...
#NikonSmallWorld #Microscopy #ScienceArt
October 3, 2025 at 9:06 AM
My entry for today’s #FluorescenceFriday: a pupal #Drosophila testis with muscles expressing
🔵 lifeact &
🔴 RFP-nls
Honored & grateful to receive an honorable mention at @healthcare.nikon.com Nikon Small World 🌍🔬✨
🔗 www.nikonsmallworld.com/galleries/20...
#NikonSmallWorld #Microscopy #ScienceArt
🔵 lifeact &
🔴 RFP-nls
Honored & grateful to receive an honorable mention at @healthcare.nikon.com Nikon Small World 🌍🔬✨
🔗 www.nikonsmallworld.com/galleries/20...
#NikonSmallWorld #Microscopy #ScienceArt
Reposted by Fillip Port
Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...
September 30, 2025 at 2:43 PM
Identification of optimal fluorophores for use in the Drosophila embryo by Timothy E Saunders and team: www.biorxiv.org/content/10.1...