Ci Ji (CJ) Lim
@cijilim.bsky.social
Associate Professor @ UW-Madison Biochemistry. www.limlab.org
Telomeres, Cryo-EM, Single-molecule Biophysics. Opinions are my own. 🇸🇬
Telomeres, Cryo-EM, Single-molecule Biophysics. Opinions are my own. 🇸🇬
Halloween pumpkin carving experiment by the Lim lab. 🎃
October 31, 2025 at 8:21 PM
Halloween pumpkin carving experiment by the Lim lab. 🎃
3/3: Similarly for human PRC2 complex, which is notoriously challenging to determine its cryo-EM structures - requires crosslinking, surface adsorption, etc -, by adding LEA proteins before plunge freezing, we determined cryo-EM maps of PRC2 at comparable resolutions to those previously reported.
February 9, 2024 at 2:48 AM
3/3: Similarly for human PRC2 complex, which is notoriously challenging to determine its cryo-EM structures - requires crosslinking, surface adsorption, etc -, by adding LEA proteins before plunge freezing, we determined cryo-EM maps of PRC2 at comparable resolutions to those previously reported.
2/3: The human DNA polymerase alpha-primase is a challenging complex to determine its structure. By simply adding nematode AavLEA1 protein to our complexes before plunge freezing, we achieved the highest-resolution cryo-EM map reported for the apo complex at 3 Å.
February 9, 2024 at 2:44 AM
2/3: The human DNA polymerase alpha-primase is a challenging complex to determine its structure. By simply adding nematode AavLEA1 protein to our complexes before plunge freezing, we achieved the highest-resolution cryo-EM map reported for the apo complex at 3 Å.
1/3: Led by @kaitlynabe.bsky.social, we found small LEA proteins from nematodes and tardigrades (water bears!) to be incredibly effective in protecting two test fragile complexes - human DNA polymerase alpha-primase and polycomb repressive complex 2 - from AWI damage during plunge freezing.
February 9, 2024 at 2:40 AM
1/3: Led by @kaitlynabe.bsky.social, we found small LEA proteins from nematodes and tardigrades (water bears!) to be incredibly effective in protecting two test fragile complexes - human DNA polymerase alpha-primase and polycomb repressive complex 2 - from AWI damage during plunge freezing.