Nicolas Bigot
@nicolab.bsky.social
Studying #DNA3R and teaching in #Paris
Reposted by Nicolas Bigot
Thanks so much for highlighting our study! The tool is versatile and ready to uncover so much new knowledge 😀
🌄 We'd like to suggest a great start into a new week with this highlighted study #Highlights2025
📕 A CRISPR-Cas9-based system for the dose-dependent study of DNA double-strand break sensing and repair
🧬 Discover the article, here ➡️ buff.ly/kDp40pZ
#CRISPR #DNAbreak #DNArepair
📕 A CRISPR-Cas9-based system for the dose-dependent study of DNA double-strand break sensing and repair
🧬 Discover the article, here ➡️ buff.ly/kDp40pZ
#CRISPR #DNAbreak #DNArepair
February 16, 2026 at 9:08 PM
Thanks so much for highlighting our study! The tool is versatile and ready to uncover so much new knowledge 😀
Reposted by Nicolas Bigot
I am happy to share our latest publication.
In this work, we show that OGG1 association with DNA during lesion search involves local DNA distortion promoting base unstacking. This process is tightly controlled by conserved residues to ensure stable association without compromising fast scanning.
In this work, we show that OGG1 association with DNA during lesion search involves local DNA distortion promoting base unstacking. This process is tightly controlled by conserved residues to ensure stable association without compromising fast scanning.
A coordinated haptic mechanism ensures efficient DNA sampling by the 8-oxoguanine glycosylase OGG1
A coordinated haptic mechanism ensures efficient DNA sampling by the 8-oxoguanine glycosylase OGG1
Martinez et al. propose that OGG1 association with DNA during lesion seeking involves local DNA distortion promoting base unstacking. This process is tightly controlled by several conserved amino acids encircling the double helix, ensuring a sufficiently stable association during base interrogation while avoiding excessive engagement detrimental for fast scanning.
dlvr.it
February 12, 2026 at 9:33 AM
I am happy to share our latest publication.
In this work, we show that OGG1 association with DNA during lesion search involves local DNA distortion promoting base unstacking. This process is tightly controlled by conserved residues to ensure stable association without compromising fast scanning.
In this work, we show that OGG1 association with DNA during lesion search involves local DNA distortion promoting base unstacking. This process is tightly controlled by conserved residues to ensure stable association without compromising fast scanning.
Reposted by Nicolas Bigot
We are thrilled to share our latest publication in Cell Reports (Cell Press)!
A huge thank you to everyone involved in this work!
📖 Read the full paper here: www.cell.com/cell-reports...
A huge thank you to everyone involved in this work!
📖 Read the full paper here: www.cell.com/cell-reports...
February 12, 2026 at 10:20 AM
We are thrilled to share our latest publication in Cell Reports (Cell Press)!
A huge thank you to everyone involved in this work!
📖 Read the full paper here: www.cell.com/cell-reports...
A huge thank you to everyone involved in this work!
📖 Read the full paper here: www.cell.com/cell-reports...
Reposted by Nicolas Bigot
In this study, we reveal that OGG1’s association with DNA during lesion searching involves local DNA distortion, promoting base unstacking. This process is tightly regulated by conserved amino acids encircling the double helix, ensuring stable binding without compromising rapid scanning.
February 12, 2026 at 10:20 AM
In this study, we reveal that OGG1’s association with DNA during lesion searching involves local DNA distortion, promoting base unstacking. This process is tightly regulated by conserved amino acids encircling the double helix, ensuring stable binding without compromising rapid scanning.
Reposted by Nicolas Bigot
A coordinated haptic mechanism ensures efficient DNA sampling by the 8-oxoguanine glycosylase OGG1
A coordinated haptic mechanism ensures efficient DNA sampling by the 8-oxoguanine glycosylase OGG1
Martinez et al. propose that OGG1 association with DNA during lesion seeking involves local DNA distortion promoting base unstacking. This process is tightly controlled by several conserved amino acids encircling the double helix, ensuring a sufficiently stable association during base interrogation while avoiding excessive engagement detrimental for fast scanning.
dlvr.it
February 7, 2026 at 12:23 AM
A coordinated haptic mechanism ensures efficient DNA sampling by the 8-oxoguanine glycosylase OGG1
Reposted by Nicolas Bigot
✍️ The Cadoret Lab contributed to a new publication
📖 @natcomms.nature.com
📃 DNA methylation and lncRNA control asynchronous DNA replication at specific imprinted gene domains
🔗 www.nature.com/articles/s41...
📖 @natcomms.nature.com
📃 DNA methylation and lncRNA control asynchronous DNA replication at specific imprinted gene domains
🔗 www.nature.com/articles/s41...
January 27, 2026 at 2:40 PM
✍️ The Cadoret Lab contributed to a new publication
📖 @natcomms.nature.com
📃 DNA methylation and lncRNA control asynchronous DNA replication at specific imprinted gene domains
🔗 www.nature.com/articles/s41...
📖 @natcomms.nature.com
📃 DNA methylation and lncRNA control asynchronous DNA replication at specific imprinted gene domains
🔗 www.nature.com/articles/s41...
Reposted by Nicolas Bigot
🧬🔬@science.org A genetically encoded device for #transcriptome storage in mammalian cells | Science www.science.org/doi/10.1126/... @broadinstitute.org
A genetically encoded device for transcriptome storage in mammalian cells
Understanding how cells make decisions over time requires the ability to link past molecular states to future phenotypic outcomes. We present TimeVault, a genetically encoded system that records and s...
www.science.org
January 15, 2026 at 8:31 PM
🧬🔬@science.org A genetically encoded device for #transcriptome storage in mammalian cells | Science www.science.org/doi/10.1126/... @broadinstitute.org
Reposted by Nicolas Bigot
L'IGDR @igdrennes.bsky.social et la Faculté des Sciences de l'Université de Rennes annoncent l'ouverture d'un poste de Professeur des Universités en section CNU 65 "Régulations génétiques et épigénétiques" avec l'opportunité de rejoindre notre équipe @spartelab.bsky.social #PU #CNU65
December 18, 2025 at 9:56 AM
L'IGDR @igdrennes.bsky.social et la Faculté des Sciences de l'Université de Rennes annoncent l'ouverture d'un poste de Professeur des Universités en section CNU 65 "Régulations génétiques et épigénétiques" avec l'opportunité de rejoindre notre équipe @spartelab.bsky.social #PU #CNU65
Reposted by Nicolas Bigot
The IGDR @igdrennes.bsky.social and the Faculty of Sciences at the University of Rennes announce an opening for a University Professor position in CNU Section 65 "Genetic and Epigenetic Regulations" with the opportunity to join our team @spartelab.bsky.social #PU #CNU65.
December 18, 2025 at 9:56 AM
The IGDR @igdrennes.bsky.social and the Faculty of Sciences at the University of Rennes announce an opening for a University Professor position in CNU Section 65 "Genetic and Epigenetic Regulations" with the opportunity to join our team @spartelab.bsky.social #PU #CNU65.
L'IGDR @igdrennes.bsky.social et la Faculté des Sciences de l'Université de Rennes annoncent l'ouverture d'un poste de Professeur des Universités en section CNU 65 "Régulations génétiques et épigénétiques" avec l'opportunité de rejoindre l'équipe SP@RTE @spartelab.bsky.social #PU #CNU65
December 18, 2025 at 9:38 AM
L'IGDR @igdrennes.bsky.social et la Faculté des Sciences de l'Université de Rennes annoncent l'ouverture d'un poste de Professeur des Universités en section CNU 65 "Régulations génétiques et épigénétiques" avec l'opportunité de rejoindre l'équipe SP@RTE @spartelab.bsky.social #PU #CNU65
Reposted by Nicolas Bigot
MISO: a micro isolation method for single-particle cryo-EM grid preparation for extremely small sample amounts.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony - Nature Methods
The micro isolation (MISO) method combines microfluidics-based protein purification with cryo-EM grid preparation, enabling cryo-EM structure determination of soluble and membrane proteins starting fr...
www.nature.com
November 20, 2025 at 4:46 PM
MISO: a micro isolation method for single-particle cryo-EM grid preparation for extremely small sample amounts.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Nicolas Bigot
Please check our collaborative paper with Marcin Suskiewicz and Sebastien Huet labs on FAM118 proteins – non-canonical sirtuins that form filaments and act as NADases in human and other vertebrate cells.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Filament formation and NAD processing by noncanonical human FAM118 sirtuins - Nature Structural & Molecular Biology
Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that FAM118A/B processing of NAD involves head-to-tail filament...
www.nature.com
November 17, 2025 at 6:17 PM
Please check our collaborative paper with Marcin Suskiewicz and Sebastien Huet labs on FAM118 proteins – non-canonical sirtuins that form filaments and act as NADases in human and other vertebrate cells.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Nicolas Bigot
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
Filament formation and NAD processing by noncanonical human FAM118 sirtuins
Nature Structural & Molecular Biology - Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that...
rdcu.be
November 17, 2025 at 11:37 AM
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
Reposted by Nicolas Bigot
Agreed! This review thoughtfully and comprehensively dives into the world of HPF1. The questions at the end are on point!
Beautiful review from J. Rudolph on Histone PARylation factor 1: a review of its role in the DNA damage response url: academic.oup.com/nar/article/...
Histone PARylation factor 1: a review of its role in the DNA damage response
Abstract. Although poly-(ADP ribose) polymerase 1 (PARP1) and PARylation of histones have been known for over 50 years and have been successfully targeted
academic.oup.com
November 11, 2025 at 4:21 PM
Agreed! This review thoughtfully and comprehensively dives into the world of HPF1. The questions at the end are on point!
Reposted by Nicolas Bigot
Beautiful review from J. Rudolph on Histone PARylation factor 1: a review of its role in the DNA damage response url: academic.oup.com/nar/article/...
Histone PARylation factor 1: a review of its role in the DNA damage response
Abstract. Although poly-(ADP ribose) polymerase 1 (PARP1) and PARylation of histones have been known for over 50 years and have been successfully targeted
academic.oup.com
November 11, 2025 at 2:49 PM
Beautiful review from J. Rudolph on Histone PARylation factor 1: a review of its role in the DNA damage response url: academic.oup.com/nar/article/...
Reposted by Nicolas Bigot
#JobOffer
💼 Un poste susceptible d’être vacant de Professeur en stabilité des génomes devrait s’ouvrir pour la rentrée 2026 au sein de l’Institut Jacques Monod
➡️ Informations concernant ce poste et la procédure de candidature 🔗 www.ijm.fr/poste-de-pro...
💼 Un poste susceptible d’être vacant de Professeur en stabilité des génomes devrait s’ouvrir pour la rentrée 2026 au sein de l’Institut Jacques Monod
➡️ Informations concernant ce poste et la procédure de candidature 🔗 www.ijm.fr/poste-de-pro...
October 28, 2025 at 1:41 PM
#JobOffer
💼 Un poste susceptible d’être vacant de Professeur en stabilité des génomes devrait s’ouvrir pour la rentrée 2026 au sein de l’Institut Jacques Monod
➡️ Informations concernant ce poste et la procédure de candidature 🔗 www.ijm.fr/poste-de-pro...
💼 Un poste susceptible d’être vacant de Professeur en stabilité des génomes devrait s’ouvrir pour la rentrée 2026 au sein de l’Institut Jacques Monod
➡️ Informations concernant ce poste et la procédure de candidature 🔗 www.ijm.fr/poste-de-pro...
Reposted by Nicolas Bigot
Finally, someone has solved a real problem with AI! No more having to take a paper in the format for a journal that rejected you, and reformat it for a new journal. Well done!! formatmypaper.com
October 15, 2025 at 6:33 AM
Finally, someone has solved a real problem with AI! No more having to take a paper in the format for a journal that rejected you, and reformat it for a new journal. Well done!! formatmypaper.com
Reposted by Nicolas Bigot
Reposted by Nicolas Bigot
SPT of DNA repair proteins after cell irradiation by Ashli Kobayashi @asaki-kobayashi.bsky.social and Romane Riou. Setup by Abbelight @abbelight.bsky.social and irradiator by Errol Laser @errol-laser.bsky.social. Look at that recruitment!
#Mifobio2025 @gdrimabio.bsky.social
#Mifobio2025 @gdrimabio.bsky.social
October 13, 2025 at 2:32 PM
SPT of DNA repair proteins after cell irradiation by Ashli Kobayashi @asaki-kobayashi.bsky.social and Romane Riou. Setup by Abbelight @abbelight.bsky.social and irradiator by Errol Laser @errol-laser.bsky.social. Look at that recruitment!
#Mifobio2025 @gdrimabio.bsky.social
#Mifobio2025 @gdrimabio.bsky.social
Reposted by Nicolas Bigot
New method paper:
Bennie Lemmens et al @scilifelab.se describe 3D-SPARK, a super-resolution microscopy method that maps changes in DNA replication nanostructures in response to genetic perturbations or drugs, linking spatial cell biology with DNA synthesis dynamics
www.embopress.org/doi/full/10....
Bennie Lemmens et al @scilifelab.se describe 3D-SPARK, a super-resolution microscopy method that maps changes in DNA replication nanostructures in response to genetic perturbations or drugs, linking spatial cell biology with DNA synthesis dynamics
www.embopress.org/doi/full/10....
October 7, 2025 at 1:04 PM
New method paper:
Bennie Lemmens et al @scilifelab.se describe 3D-SPARK, a super-resolution microscopy method that maps changes in DNA replication nanostructures in response to genetic perturbations or drugs, linking spatial cell biology with DNA synthesis dynamics
www.embopress.org/doi/full/10....
Bennie Lemmens et al @scilifelab.se describe 3D-SPARK, a super-resolution microscopy method that maps changes in DNA replication nanostructures in response to genetic perturbations or drugs, linking spatial cell biology with DNA synthesis dynamics
www.embopress.org/doi/full/10....
Reposted by Nicolas Bigot
New pre-print on the role of CCDC103/DNAAF19 in dynein assembly highlighting it's link to PCD.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
CCDC103-mediated assembly of the R2C complex links RUVBL1-RUVBL2 to Primary Ciliary Dyskinesia
Primary ciliary dyskinesia (PCD) is a genetic disorder caused by defective cilia motility, powered by axonemal dynein motors. Assembly of these motors is facilitated by the molecular chaperone HSP90, ...
www.biorxiv.org
September 15, 2025 at 7:56 PM
New pre-print on the role of CCDC103/DNAAF19 in dynein assembly highlighting it's link to PCD.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Nicolas Bigot
Exciting new study from new-PI Mohinder Pal on the role of CCDC103 and it's links to PCD as the most recent dynein assembly factor (DNAAF19). Congratulations to the entire team.
@Mohinder Pal (currently not on Bluesky)
@mike-wieczorek.bsky.social
@laurencepearl.bsky.social
@Mohinder Pal (currently not on Bluesky)
@mike-wieczorek.bsky.social
@laurencepearl.bsky.social
New pre-print on the role of CCDC103/DNAAF19 in dynein assembly highlighting it's link to PCD.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
CCDC103-mediated assembly of the R2C complex links RUVBL1-RUVBL2 to Primary Ciliary Dyskinesia
Primary ciliary dyskinesia (PCD) is a genetic disorder caused by defective cilia motility, powered by axonemal dynein motors. Assembly of these motors is facilitated by the molecular chaperone HSP90, ...
www.biorxiv.org
September 15, 2025 at 8:00 PM
Exciting new study from new-PI Mohinder Pal on the role of CCDC103 and it's links to PCD as the most recent dynein assembly factor (DNAAF19). Congratulations to the entire team.
@Mohinder Pal (currently not on Bluesky)
@mike-wieczorek.bsky.social
@laurencepearl.bsky.social
@Mohinder Pal (currently not on Bluesky)
@mike-wieczorek.bsky.social
@laurencepearl.bsky.social
Reposted by Nicolas Bigot
Academic research generates almost all intellecutal innovation in the life sciences. With big pharma refusing to contribute, maybe it's time to stop giving them our research outputs cheaply, and instead having globally nationalised means of translating innovation?
www.theguardian.com/business/202...
www.theguardian.com/business/202...
US drugmaker Merck scraps £1bn London research centre and cuts 125 science jobs
New blow to UK’s key life science sector as industry body says country is losing ground on investment and research
www.theguardian.com
September 11, 2025 at 7:52 AM
Academic research generates almost all intellecutal innovation in the life sciences. With big pharma refusing to contribute, maybe it's time to stop giving them our research outputs cheaply, and instead having globally nationalised means of translating innovation?
www.theguardian.com/business/202...
www.theguardian.com/business/202...
Reposted by Nicolas Bigot
I supervise an excellent postdoc who's looking for a postdoc or associate position in < 1 yr. Highly skilled in chem biol and biochem (peptide synthesis, native chem ligation, bacterial expression, purification, intein-based splicing, crystallography, interaction assays). Contact me if interested.
September 5, 2025 at 5:41 AM
I supervise an excellent postdoc who's looking for a postdoc or associate position in < 1 yr. Highly skilled in chem biol and biochem (peptide synthesis, native chem ligation, bacterial expression, purification, intein-based splicing, crystallography, interaction assays). Contact me if interested.
Reposted by Nicolas Bigot
#New-Paper Rass laboratory reveal DNA2 to be a gatekeeper to homologous recombination at stalled DNA replication forks, rrequired to stave off cellular senescence. Their findings provide a molecular explanation for the association of DNA2 with primordial dwarfism and cancer.
doi.org/10.1038/s415...
doi.org/10.1038/s415...
DNA2 enables growth by restricting recombination-restarted replication - Nature
DNA2 suppresses recombination-restarted replication and checkpoint activation at stalled forks, and its loss triggers recombination-dependent synthesis, checkpoint signalling and cell-cycle exit, high...
doi.org
September 3, 2025 at 3:13 PM
#New-Paper Rass laboratory reveal DNA2 to be a gatekeeper to homologous recombination at stalled DNA replication forks, rrequired to stave off cellular senescence. Their findings provide a molecular explanation for the association of DNA2 with primordial dwarfism and cancer.
doi.org/10.1038/s415...
doi.org/10.1038/s415...