Basavraj Khanppnavar
@basurajsk.bsky.social
Scientist @Paul Scherrer Institute. Interested in #membrane-associated #signalling complexes and #host-pathogen interactions #Structural Biology
Pinned
Want to know “How does #Botox enter cells at the right time & place to exert its #therapeutic effect?” Check out our latest paper @naturecomms.bsky.social @psich_en @KorkhovLab @ethzurich.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Basavraj Khanppnavar
The best cover🥰, paired cats & paired helical filaments.
July 17, 2025 at 6:47 PM
The best cover🥰, paired cats & paired helical filaments.
Reposted by Basavraj Khanppnavar
In our latest preprint on bioRxiv, we present the in situ structure of
the human gap junction at 14 Å resolution using cryo-ET.
The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.
the human gap junction at 14 Å resolution using cryo-ET.
The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.
July 8, 2025 at 5:33 AM
In our latest preprint on bioRxiv, we present the in situ structure of
the human gap junction at 14 Å resolution using cryo-ET.
The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.
the human gap junction at 14 Å resolution using cryo-ET.
The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.
Reposted by Basavraj Khanppnavar
Ever wondered how stable your ADCs are and how their stability is influenced by their drug-load?
Variable-temperature electrospray ionization can help with that!
Jan Fiala’s and my postdoc work in the lab of @hecklab.bsky.social was just published! 🥳🥳🥳
pubs.acs.org/doi/10.1021/...
1/3
Variable-temperature electrospray ionization can help with that!
Jan Fiala’s and my postdoc work in the lab of @hecklab.bsky.social was just published! 🥳🥳🥳
pubs.acs.org/doi/10.1021/...
1/3
Antibody–Drug Conjugate Stability Probed by Variable-Temperature Electrospray Ionization Mass Spectrometry
Antibody–drug conjugates (ADCs) are effective anticancer biotherapeutics, often referred to as “magic bullets” due to their high specificity and cytotoxicity. This unique drug class consists of cytoto...
pubs.acs.org
May 23, 2025 at 6:52 PM
Ever wondered how stable your ADCs are and how their stability is influenced by their drug-load?
Variable-temperature electrospray ionization can help with that!
Jan Fiala’s and my postdoc work in the lab of @hecklab.bsky.social was just published! 🥳🥳🥳
pubs.acs.org/doi/10.1021/...
1/3
Variable-temperature electrospray ionization can help with that!
Jan Fiala’s and my postdoc work in the lab of @hecklab.bsky.social was just published! 🥳🥳🥳
pubs.acs.org/doi/10.1021/...
1/3
Reposted by Basavraj Khanppnavar
I am delighted to share our findings on the binding mode of tetanus neurotoxin and the anti-epileptic drug Levetiracetam to SV2A, an essential synaptic vesicle membrane protein of unknown function, now online in @NatComms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structures of native SV2A reveal the binding mode for tetanus neurotoxin and anti-epileptic racetams - Nature Communications
SV2A, an essential membrane protein in neurotransmission, is known as the receptor of clostridial neurotoxins and target of anti-epileptic drugs. Here the authors show details of the binding site for ...
www.nature.com
May 5, 2025 at 6:13 PM
I am delighted to share our findings on the binding mode of tetanus neurotoxin and the anti-epileptic drug Levetiracetam to SV2A, an essential synaptic vesicle membrane protein of unknown function, now online in @NatComms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Basavraj Khanppnavar
#CryoEM #structure of the botulinum neurotoxin A/SV2B complex and its implications for translocation. @NatureComms Check this #membrane #protein in the UniTmp database: https://pdbtm.unitmp.org/entry/9f1r
www.nature.com
March 24, 2025 at 8:00 PM
#CryoEM #structure of the botulinum neurotoxin A/SV2B complex and its implications for translocation. @NatureComms Check this #membrane #protein in the UniTmp database: https://pdbtm.unitmp.org/entry/9f1r
Reposted by Basavraj Khanppnavar
Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6
March 21, 2025 at 3:16 PM
Asgard archaea have actin - but what about microtubules? Where do they come from? 🧐 Our new paper www.cell.com/cell/fulltex... by @xujwet.bsky.social & @florianwollweber.bsky.social, in collaboration with the Schleper & Wieczorek labs, describes tiny Asgard microtubules! #TeamTomo #ArchaeaSky 1/6
Delighted to share #AuthorInterview with @biopatrika.bsky.social were we discuss on recent findings on Botox.
biopatrika.com/academia/res...
biopatrika.com/academia/res...
Molecular Snapshots Unravel Botox’s Secrets of Nerve Cell Entry - BioPatrika
Researcher Spotlight - In our study, we demonstrate for the first time the existence of large conformational changes in Botox and explain their purpose.
biopatrika.com
March 20, 2025 at 9:21 AM
Delighted to share #AuthorInterview with @biopatrika.bsky.social were we discuss on recent findings on Botox.
biopatrika.com/academia/res...
biopatrika.com/academia/res...
Reposted by Basavraj Khanppnavar
Coot 1.1.14 has been released!
Coming to homebrew and flatpak soon!
- Clark & Labute comes to Coot 1, Moorhen & chapi!
(svg, 2D ligand environment view)
- Solid Symmetry (symmetry that you can see!)
- PAE plot for AlphaFold models
#coot
#molmatinf
Coming to homebrew and flatpak soon!
- Clark & Labute comes to Coot 1, Moorhen & chapi!
(svg, 2D ligand environment view)
- Solid Symmetry (symmetry that you can see!)
- PAE plot for AlphaFold models
#coot
#molmatinf
March 17, 2025 at 7:48 PM
Coot 1.1.14 has been released!
Coming to homebrew and flatpak soon!
- Clark & Labute comes to Coot 1, Moorhen & chapi!
(svg, 2D ligand environment view)
- Solid Symmetry (symmetry that you can see!)
- PAE plot for AlphaFold models
#coot
#molmatinf
Coming to homebrew and flatpak soon!
- Clark & Labute comes to Coot 1, Moorhen & chapi!
(svg, 2D ligand environment view)
- Solid Symmetry (symmetry that you can see!)
- PAE plot for AlphaFold models
#coot
#molmatinf
Reposted by Basavraj Khanppnavar
Happy to share our latest publication on
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv
Mutant tau filaments from some inherited frontotemporal dementias show the Alzheimer fold
Nature Structural & Molecular Biology - Frontotemporal dementias can be caused by mutations in MAPT, which encodes the protein tau; the mutant protein forms harmful aggregates in the brain....
rdcu.be
March 13, 2025 at 1:50 PM
Happy to share our latest publication on
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv
Reposted by Basavraj Khanppnavar
Very proud of our new review article on the Beta 1-Adrenergic GPCR now available on bioRxiv: www.biorxiv.org/content/10.1....
With @saureli.bsky.social @valeriorizzi.bsky.social and Nicola we used OneOPES to fully converge the free energy landscapes associated with B1AR apo/holo activation.
With @saureli.bsky.social @valeriorizzi.bsky.social and Nicola we used OneOPES to fully converge the free energy landscapes associated with B1AR apo/holo activation.
March 12, 2025 at 1:27 PM
Very proud of our new review article on the Beta 1-Adrenergic GPCR now available on bioRxiv: www.biorxiv.org/content/10.1....
With @saureli.bsky.social @valeriorizzi.bsky.social and Nicola we used OneOPES to fully converge the free energy landscapes associated with B1AR apo/holo activation.
With @saureli.bsky.social @valeriorizzi.bsky.social and Nicola we used OneOPES to fully converge the free energy landscapes associated with B1AR apo/holo activation.
Reposted by Basavraj Khanppnavar
Nature research paper: Natural behaviour is learned through dopamine-mediated reinforcement
https://go.nature.com/3XMKATn
https://go.nature.com/3XMKATn
Natural behaviour is learned through dopamine-mediated reinforcement - Nature
Studies in zebra finches show that dopamine has a key role as a reinforcement signal in the trial-and-error process of learning that underlies complex natural behaviours.
go.nature.com
March 12, 2025 at 7:02 PM
Nature research paper: Natural behaviour is learned through dopamine-mediated reinforcement
https://go.nature.com/3XMKATn
https://go.nature.com/3XMKATn
Reposted by Basavraj Khanppnavar
Male blue-lined octopuses inject females with venom during mating to avoid being eaten by their partners
https://go.nature.com/3FqtOU4
https://go.nature.com/3FqtOU4
Bad romance: male octopuses inject deadly venom into their mates
The paralysing toxin deployed by the male blue-lined octopus might help to protect him from being eaten.
go.nature.com
March 12, 2025 at 12:25 PM
Male blue-lined octopuses inject females with venom during mating to avoid being eaten by their partners
https://go.nature.com/3FqtOU4
https://go.nature.com/3FqtOU4
Reposted by Basavraj Khanppnavar
Happy to share the beautiful structure of a Wadjet SMC complex by @roisnehamelinf.bsky.social, with support from the DCI and @drhonsworth.bsky.social ! This is a type II Wadjet with unique characteristics including a tandem KITE subunit, distinct coiled coil architecture and a deviant hinge.
March 11, 2025 at 9:22 AM
Happy to share the beautiful structure of a Wadjet SMC complex by @roisnehamelinf.bsky.social, with support from the DCI and @drhonsworth.bsky.social ! This is a type II Wadjet with unique characteristics including a tandem KITE subunit, distinct coiled coil architecture and a deviant hinge.
Well said!!. I totally support this. As a collaborator or teamate and co coauthor, it's also our responsibility to ensure quality of Science in every publication we publish as coauthor. #SaveScienceFromJunk
If you do an experiment for someone else's paper and you become a co-author, this means that you agree with *everything* in that paper! It takes time and effort to reach consensus, but this ensures better science. That's why I cannot understand how some people publish so many papers each year.
March 10, 2025 at 7:52 AM
Well said!!. I totally support this. As a collaborator or teamate and co coauthor, it's also our responsibility to ensure quality of Science in every publication we publish as coauthor. #SaveScienceFromJunk
Reposted by Basavraj Khanppnavar
Hey kiddos. Don’t forget to filter your buffers and samples (any cut off is better than no cutoff) or use an inline filter on your systems!
March 5, 2025 at 10:46 PM
Hey kiddos. Don’t forget to filter your buffers and samples (any cut off is better than no cutoff) or use an inline filter on your systems!
Reposted by Basavraj Khanppnavar
We have deposited the following mice (all B6/J backcrossed) to @mmrrc.bsky.social
Adgrf5-EGFP-CT-IRES-CRE
GPR123- EGFP-CT-IRES-CRE
GPR124- EGFP-CT-IRES-CRE
GPR125- EGFP-CT-IRES-CRE
Gpr133-EGFP-CT-IRES-CRE
GPR156-IRES-Cre
GPR156-mGL-IRES-Cre
GPR75-IRES-Cre
Adgrf5-EGFP-CT-IRES-CRE
GPR123- EGFP-CT-IRES-CRE
GPR124- EGFP-CT-IRES-CRE
GPR125- EGFP-CT-IRES-CRE
Gpr133-EGFP-CT-IRES-CRE
GPR156-IRES-Cre
GPR156-mGL-IRES-Cre
GPR75-IRES-Cre
February 26, 2025 at 4:44 PM
We have deposited the following mice (all B6/J backcrossed) to @mmrrc.bsky.social
Adgrf5-EGFP-CT-IRES-CRE
GPR123- EGFP-CT-IRES-CRE
GPR124- EGFP-CT-IRES-CRE
GPR125- EGFP-CT-IRES-CRE
Gpr133-EGFP-CT-IRES-CRE
GPR156-IRES-Cre
GPR156-mGL-IRES-Cre
GPR75-IRES-Cre
Adgrf5-EGFP-CT-IRES-CRE
GPR123- EGFP-CT-IRES-CRE
GPR124- EGFP-CT-IRES-CRE
GPR125- EGFP-CT-IRES-CRE
Gpr133-EGFP-CT-IRES-CRE
GPR156-IRES-Cre
GPR156-mGL-IRES-Cre
GPR75-IRES-Cre
Reposted by Basavraj Khanppnavar
The paper by @BasurajSK, Oneda Leka, Pal Sushant, @KorkhovLab, and Richard Kammerer (@psich_en), published in @NatureComms provides molecular insights in botulinum neurotoxin recognition and pH-dependent conformational transitions relevant to its mechanism of action. biol.ethz.ch/en/news-and-...
February 18, 2025 at 7:42 AM
The paper by @BasurajSK, Oneda Leka, Pal Sushant, @KorkhovLab, and Richard Kammerer (@psich_en), published in @NatureComms provides molecular insights in botulinum neurotoxin recognition and pH-dependent conformational transitions relevant to its mechanism of action. biol.ethz.ch/en/news-and-...
Reposted by Basavraj Khanppnavar
Symbiotic #bacteria ride along with single-celled #protists in ocean's upper layer phys.org/news/2025-02...
#Symbionts of predatory protists are widespread in the oceans and related to animal pathogens www.cell.com/cell-host-mi...
#Symbionts of predatory protists are widespread in the oceans and related to animal pathogens www.cell.com/cell-host-mi...
February 13, 2025 at 6:43 AM
Symbiotic #bacteria ride along with single-celled #protists in ocean's upper layer phys.org/news/2025-02...
#Symbionts of predatory protists are widespread in the oceans and related to animal pathogens www.cell.com/cell-host-mi...
#Symbionts of predatory protists are widespread in the oceans and related to animal pathogens www.cell.com/cell-host-mi...
Reposted by Basavraj Khanppnavar
Cryo-EM structure of the botulinum neurotoxin A/SV2B complex and its implications for translocation pubmed.ncbi.nlm.nih.gov/39934119/ #cryoem
February 12, 2025 at 3:50 PM
Cryo-EM structure of the botulinum neurotoxin A/SV2B complex and its implications for translocation pubmed.ncbi.nlm.nih.gov/39934119/ #cryoem
Reposted by Basavraj Khanppnavar
Cryo-EM structure of the botulinum neurotoxin A/SV2B complex and its implications for translocation
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cryo-EM structure of the botulinum neurotoxin A/SV2B complex and its implications for translocation - Nature Communications
Translocation into neurons is crucial for BoNT/A1 action. Here, the authors show by single-particle cryo-EM that BoNT/A1 bound to its receptor SV2B switches its conformation from an open, translocatio...
www.nature.com
February 11, 2025 at 12:42 PM
Cryo-EM structure of the botulinum neurotoxin A/SV2B complex and its implications for translocation
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Want to know “How does #Botox enter cells at the right time & place to exert its #therapeutic effect?” Check out our latest paper @naturecomms.bsky.social @psich_en @KorkhovLab @ethzurich.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
February 11, 2025 at 12:24 PM
Want to know “How does #Botox enter cells at the right time & place to exert its #therapeutic effect?” Check out our latest paper @naturecomms.bsky.social @psich_en @KorkhovLab @ethzurich.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Basavraj Khanppnavar
Love this.. 👏
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
January 15, 2025 at 4:23 PM
Love this.. 👏
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
Reposted by Basavraj Khanppnavar
Looking for a primer on why the contrast transfer function matters in #cryoEM?
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
December 9, 2024 at 9:24 PM
Looking for a primer on why the contrast transfer function matters in #cryoEM?
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...
Reposted by Basavraj Khanppnavar
How can medicines be directed to the precise location within the body where they need to act? Research lead by ETH Zurich have now unveiled a special class of effective particles. They appear visually striking under a microscope too, resembling tiny paper flowers or desert roses.
Delivering medicines with microscopic flowers
These small particles are reminiscent of paper flowers or desert roses. Physicians can use them to guide medicines to a precise destination within the body. Better yet, the particles can easily be tra...
ethz.ch
December 10, 2024 at 8:44 AM
How can medicines be directed to the precise location within the body where they need to act? Research lead by ETH Zurich have now unveiled a special class of effective particles. They appear visually striking under a microscope too, resembling tiny paper flowers or desert roses.