Janine Brunner
@janinebrunner.bsky.social
Group Leader VIB and Assistant Professor VUB Brussels, Belgium - Biochemist - Structural biologist - interested in #MembraneProteins #cryoEM - nature - paragliding
Reposted by Janine Brunner
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
October 30, 2025 at 3:21 AM
New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz
Reposted by Janine Brunner
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
October 29, 2025 at 5:11 PM
Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
Happy to announce our most recent preprint: Stereoselectivity and plasticity of a common binding pocket in TRPM3, by cryo-EM and elecrophysiology. Great collaboration with the Voets, Vriens and CISTIM labs.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
August 28, 2025 at 6:39 PM
Happy to announce our most recent preprint: Stereoselectivity and plasticity of a common binding pocket in TRPM3, by cryo-EM and elecrophysiology. Great collaboration with the Voets, Vriens and CISTIM labs.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Janine Brunner
Happy to share the latest from the lab, led by Daniel Alvarez, in collaboration with @lizconibear.bsky.social. In this AA-MD tour-de-force, we delve deep into the mechanism and energetics of lipid uptake by bridge-like lipid transfer proteins, and we learn a few interesting things along the way...
August 7, 2025 at 8:45 AM
Happy to share the latest from the lab, led by Daniel Alvarez, in collaboration with @lizconibear.bsky.social. In this AA-MD tour-de-force, we delve deep into the mechanism and energetics of lipid uptake by bridge-like lipid transfer proteins, and we learn a few interesting things along the way...
Reposted by Janine Brunner
We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
August 5, 2025 at 12:41 PM
We are excited to announce the first in-cell structure of a LINC complex within a native nuclear envelope at subnanometer resolution!! #In-cell cryo-ET + #atomistic MD simulations! @tomdendooven.bsky.social et al!!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Janine Brunner
Happy to share the latest work from the lab, led by @mudgal17.bsky.social, in collaboration with the Weis lab @ethzurich.bsky.social.
How do nuclear membranes fuse during NPC assembly? We answer this question in our latest work, where we identify a new mechanism for membrane fusion… (1/13)
How do nuclear membranes fuse during NPC assembly? We answer this question in our latest work, where we identify a new mechanism for membrane fusion… (1/13)
July 23, 2025 at 11:40 AM
Happy to share the latest work from the lab, led by @mudgal17.bsky.social, in collaboration with the Weis lab @ethzurich.bsky.social.
How do nuclear membranes fuse during NPC assembly? We answer this question in our latest work, where we identify a new mechanism for membrane fusion… (1/13)
How do nuclear membranes fuse during NPC assembly? We answer this question in our latest work, where we identify a new mechanism for membrane fusion… (1/13)
Reposted by Janine Brunner
New preprint in the lab combining what we like best: insects, love, chemistry and the olfactory system! 1/8
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
A covalent recognition strategy enables conspecific mate identification
The olfactory system can detect an uncountable number of volatile molecules while retaining the ability to discriminate amongst very similar ones. We identified a unique mechanism employed by insect o...
www.biorxiv.org
June 3, 2025 at 9:59 PM
New preprint in the lab combining what we like best: insects, love, chemistry and the olfactory system! 1/8
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Janine Brunner
Excited to share our latest story in Nature. We developed a high-throughput cellular stretch system to look for genes regulating membrane rupture under tension, and found an amazing small protein called NINJ1 that weakens the membrane for rupture! H/T to Zozo, my 10-y.o. for her artist's impression😃
June 9, 2025 at 3:19 PM
Excited to share our latest story in Nature. We developed a high-throughput cellular stretch system to look for genes regulating membrane rupture under tension, and found an amazing small protein called NINJ1 that weakens the membrane for rupture! H/T to Zozo, my 10-y.o. for her artist's impression😃
Reposted by Janine Brunner
Check out this remarkable debut paper from @jie-xu.bsky.social, a very creative lab alumnus. Very proud!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
June 9, 2025 at 3:30 PM
Check out this remarkable debut paper from @jie-xu.bsky.social, a very creative lab alumnus. Very proud!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Janine Brunner
I’m thrilled to finally share this preprint! It contains a WOW Cryo-EM structure (likely one of the strongest protein fibers known to man 🤯), but we’ve also untangled its biological function: a novel type of virulence factor💀!
www.researchsquare.com/article/rs-6...
www.researchsquare.com/article/rs-6...
May 20, 2025 at 9:37 AM
I’m thrilled to finally share this preprint! It contains a WOW Cryo-EM structure (likely one of the strongest protein fibers known to man 🤯), but we’ve also untangled its biological function: a novel type of virulence factor💀!
www.researchsquare.com/article/rs-6...
www.researchsquare.com/article/rs-6...
Reposted by Janine Brunner
In our latest preprint we used cryoEM to solve the structure of A-ENA fibers and show that they are stabilized by 10 isopeptide bonds per monomer. A-ENA couples the spore to the cry-toxins, and in doing so increases the virulence of Bacillus thuringiensis: www.researchsquare.com/article/rs-6...
May 20, 2025 at 9:22 AM
In our latest preprint we used cryoEM to solve the structure of A-ENA fibers and show that they are stabilized by 10 isopeptide bonds per monomer. A-ENA couples the spore to the cry-toxins, and in doing so increases the virulence of Bacillus thuringiensis: www.researchsquare.com/article/rs-6...
I am delighted to share our findings on the binding mode of tetanus neurotoxin and the anti-epileptic drug Levetiracetam to SV2A, an essential synaptic vesicle membrane protein of unknown function, now online in @NatComms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structures of native SV2A reveal the binding mode for tetanus neurotoxin and anti-epileptic racetams - Nature Communications
SV2A, an essential membrane protein in neurotransmission, is known as the receptor of clostridial neurotoxins and target of anti-epileptic drugs. Here the authors show details of the binding site for ...
www.nature.com
May 5, 2025 at 6:13 PM
I am delighted to share our findings on the binding mode of tetanus neurotoxin and the anti-epileptic drug Levetiracetam to SV2A, an essential synaptic vesicle membrane protein of unknown function, now online in @NatComms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Janine Brunner
Please RT. Post-doc opportunity alert! 💥 Come join our team (thelowlab.org) at Imperial, London, working on bacterial secretion systems. The position is funded by the Wellcome Trust.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
March 28, 2025 at 5:39 PM
Please RT. Post-doc opportunity alert! 💥 Come join our team (thelowlab.org) at Imperial, London, working on bacterial secretion systems. The position is funded by the Wellcome Trust.
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
For more details and to apply please see
www.imperial.ac.uk/jobs/search-...
Reposted by Janine Brunner
Here's our newest study: we reveal the molecular principles of neuronal excitation by glutamate, and how physiological temperatures influence this process.
www.nature.com/articles/s41...
www.nature.com/articles/d41...
Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/d41...
Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com
March 26, 2025 at 4:29 PM
Here's our newest study: we reveal the molecular principles of neuronal excitation by glutamate, and how physiological temperatures influence this process.
www.nature.com/articles/s41...
www.nature.com/articles/d41...
Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/d41...
Led by @anish-mondal07.bsky.social, published in @natureportfolio.nature.com
Reposted by Janine Brunner
Structural basis of ClC-3 inhibition by TMEM9 and PI(3,5)P 2 pubmed.ncbi.nlm.nih.gov/40093093/ #cryoem
March 18, 2025 at 7:45 PM
Structural basis of ClC-3 inhibition by TMEM9 and PI(3,5)P 2 pubmed.ncbi.nlm.nih.gov/40093093/ #cryoem
Reposted by Janine Brunner
Happy to share our latest publication on
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv
Mutant tau filaments from some inherited frontotemporal dementias show the Alzheimer fold
Nature Structural & Molecular Biology - Frontotemporal dementias can be caused by mutations in MAPT, which encodes the protein tau; the mutant protein forms harmful aggregates in the brain....
rdcu.be
March 13, 2025 at 1:50 PM
Happy to share our latest publication on
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv
@naturesmb.bsky.social Natural Structure & Molecular Biology, we show tau filaments show Alzheimer fold in mutants V337M and R406W.
thanks @sjorsscheres.bsky.social and Michel Goedert's support. 🥳
rdcu.be/edqzW
rdcu.be/edoGv
Reposted by Janine Brunner
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
March 13, 2025 at 7:20 PM
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
Reposted by Janine Brunner
Themis and Grb2 form a constitutive structural hub in TCR signaling
doi.org/10.1101/2025...
@savvideslab.bsky.social
doi.org/10.1101/2025...
@savvideslab.bsky.social
February 23, 2025 at 2:39 AM
Themis and Grb2 form a constitutive structural hub in TCR signaling
doi.org/10.1101/2025...
@savvideslab.bsky.social
doi.org/10.1101/2025...
@savvideslab.bsky.social
Reposted by Janine Brunner
Just because it's a homo-oligomer, don't assume it's symmetric! @benjabsnt.bsky.social & collaborators used cryo-EM to study the transthyretin (TTR) tetramer (55 kDa), shedding light on its misfolding pathways linked to amyloid disease. Now in peer-reviewed form: www.nature.com/articles/s41...
January 22, 2025 at 4:45 PM
Just because it's a homo-oligomer, don't assume it's symmetric! @benjabsnt.bsky.social & collaborators used cryo-EM to study the transthyretin (TTR) tetramer (55 kDa), shedding light on its misfolding pathways linked to amyloid disease. Now in peer-reviewed form: www.nature.com/articles/s41...
Reposted by Janine Brunner
In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
January 17, 2025 at 6:43 PM
In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
Reposted by Janine Brunner
Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...
January 16, 2025 at 10:57 PM
Protein production for cryoEM structure determination just got a lot cheaper - high resolution structure from half a plate of HEK cells with the "MIcro ISolation (MISO)" microfluidics-based approach. Congrats to the Brunner and Efremov labs in Brussels! www.biorxiv.org/content/10.1...
Cryo-EM structures of mammalian membrane proteins from a single cell culture dish?
Yes, Micro ISOlation (MISO) sample preparation makes it possible.
www.biorxiv.org/content/10.1...
Happy to be part of this exciting story and wonderful collaboration with the Rouslan Efremov lab.
Yes, Micro ISOlation (MISO) sample preparation makes it possible.
www.biorxiv.org/content/10.1...
Happy to be part of this exciting story and wonderful collaboration with the Rouslan Efremov lab.
January 16, 2025 at 9:27 AM
Cryo-EM structures of mammalian membrane proteins from a single cell culture dish?
Yes, Micro ISOlation (MISO) sample preparation makes it possible.
www.biorxiv.org/content/10.1...
Happy to be part of this exciting story and wonderful collaboration with the Rouslan Efremov lab.
Yes, Micro ISOlation (MISO) sample preparation makes it possible.
www.biorxiv.org/content/10.1...
Happy to be part of this exciting story and wonderful collaboration with the Rouslan Efremov lab.
Reposted by Janine Brunner
Love this.. 👏
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
January 15, 2025 at 4:23 PM
Love this.. 👏
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
www.biorxiv.org/content/10.1...