Sylvie Callegari
@sylviecallegari.bsky.social
Postdoc @Komander lab, WEHI, Melbourne.
Mitochondrial biochemist interested in ubiquitin 🤓.
Mitochondrial biochemist interested in ubiquitin 🤓.
Pinned
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
We’re hiring a research assistant! If you have experience in protein production and purification (bacterial and/or insect systems) and are interested in early-stage drug discovery, please apply! 👇 wehi.wd3.myworkdayjobs.com/broadbean_ex....
Research Assistant
Job Title Research Assistant Job Description An exciting opportunity exists for an experienced Research Assistant to work with Dr. Sylvie Callegari and Prof. David Komander within the Ubiquitin Signal...
wehi.wd3.myworkdayjobs.com
October 20, 2025 at 10:51 AM
We’re hiring a research assistant! If you have experience in protein production and purification (bacterial and/or insect systems) and are interested in early-stage drug discovery, please apply! 👇 wehi.wd3.myworkdayjobs.com/broadbean_ex....
Reposted by Sylvie Callegari
How can mitophagy be an effective quality control mechanism if mtDNA mutations reach high enough levels to cause disease?
This question led us into a dark path, full of concepts of evolutionary genetics, germline stem cell biology and mito-nuclear compatibility.
www.science.org/doi/10.1126/...
This question led us into a dark path, full of concepts of evolutionary genetics, germline stem cell biology and mito-nuclear compatibility.
www.science.org/doi/10.1126/...
Ubiquitin-mediated mitophagy regulates the inheritance of mitochondrial DNA mutations
Mitochondrial synthesis of adenosine triphosphate is essential for eukaryotic life but is dependent on the cooperation of two genomes: nuclear and mitochondrial DNA (mtDNA). mtDNA mutates ~15 times as...
www.science.org
October 10, 2025 at 8:45 AM
How can mitophagy be an effective quality control mechanism if mtDNA mutations reach high enough levels to cause disease?
This question led us into a dark path, full of concepts of evolutionary genetics, germline stem cell biology and mito-nuclear compatibility.
www.science.org/doi/10.1126/...
This question led us into a dark path, full of concepts of evolutionary genetics, germline stem cell biology and mito-nuclear compatibility.
www.science.org/doi/10.1126/...
Really enjoyed the #ASBMB2025 conference in Brisbane this week. It's an honour to get to present to this audience and winning an oral presentation prize was an unexpected bonus!
Congratulations to all our poster and speaker prize winners!! 🏆🏆🏆
October 3, 2025 at 1:45 AM
Really enjoyed the #ASBMB2025 conference in Brisbane this week. It's an honour to get to present to this audience and winning an oral presentation prize was an unexpected bonus!
Reposted by Sylvie Callegari
I am thrilled to release ProteinDJ: a high-performance and modular protein design pipeline. Our open-source workflow incorporates #RFdiffusion, #ProteinMPNN, #FAMPNN, #AlphaFold2 and #Boltz-2. It is a fast, free, and fun way to design proteins (1/5)
doi.org/10.1101/2025.09.24.678028 #proteindesign
doi.org/10.1101/2025.09.24.678028 #proteindesign
September 28, 2025 at 9:16 PM
I am thrilled to release ProteinDJ: a high-performance and modular protein design pipeline. Our open-source workflow incorporates #RFdiffusion, #ProteinMPNN, #FAMPNN, #AlphaFold2 and #Boltz-2. It is a fast, free, and fun way to design proteins (1/5)
doi.org/10.1101/2025.09.24.678028 #proteindesign
doi.org/10.1101/2025.09.24.678028 #proteindesign
Reposted by Sylvie Callegari
Researchers from WEHI’s Parkinson Disease Research Centre have won the 2025 UNSW Eureka Prize for Scientific Research. The team has been honoured for their research unravelling how PINK1, a key protein linked to early-onset Parkinson’s disease, works.
🔗 www.wehi.edu.au/news/eureka-...
🔗 www.wehi.edu.au/news/eureka-...
September 3, 2025 at 9:54 PM
Researchers from WEHI’s Parkinson Disease Research Centre have won the 2025 UNSW Eureka Prize for Scientific Research. The team has been honoured for their research unravelling how PINK1, a key protein linked to early-onset Parkinson’s disease, works.
🔗 www.wehi.edu.au/news/eureka-...
🔗 www.wehi.edu.au/news/eureka-...
Reposted by Sylvie Callegari
Very exciting to see the @wehi-research.bsky.social PINK1 Parkinson’s Disease research team win the Eureka Prize for Scientific Research. Amazing achievement by a brilliant team! @eurekaprizes.bsky.social
September 3, 2025 at 10:00 AM
Very exciting to see the @wehi-research.bsky.social PINK1 Parkinson’s Disease research team win the Eureka Prize for Scientific Research. Amazing achievement by a brilliant team! @eurekaprizes.bsky.social
Reposted by Sylvie Callegari
The Institute for Molecular Bioscience is seeking an exceptional academic leader to be the new Director of the Centre for Chemistry and Drug Discovery and lead our centre consisting of 10 group leaders, 40 staff and approximately 50 HDR students. Come and join us in sunny Brisbane!
Director, Centre for Chemistry and Drug Discovery
Institute for Molecular Bioscience Join a university ranked in the world’s top 50 Australia’s #1 research institute Based at St Lucia Campus About UQ What started as Queensland's first university in 1...
uq.wd3.myworkdayjobs.com
September 2, 2025 at 2:46 AM
The Institute for Molecular Bioscience is seeking an exceptional academic leader to be the new Director of the Centre for Chemistry and Drug Discovery and lead our centre consisting of 10 group leaders, 40 staff and approximately 50 HDR students. Come and join us in sunny Brisbane!
Reposted by Sylvie Callegari
Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...
August 28, 2025 at 6:54 PM
Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...
Reposted by Sylvie Callegari
Our June Newsletter highlights IUBMB activities including a note from the President and updates on the IUBMB Trainee Initiative
➡️http://bit.ly/45ai6r1
➡️http://bit.ly/45ai6r1
August 8, 2025 at 3:17 PM
Our June Newsletter highlights IUBMB activities including a note from the President and updates on the IUBMB Trainee Initiative
➡️http://bit.ly/45ai6r1
➡️http://bit.ly/45ai6r1
Reposted by Sylvie Callegari
PINK1 Parkinson's Disease Research Team @wehi-research.bsky.social is a finalist in the @unsw.edu.au Eureka Prize for Scientific Research.
Learn more: youtu.be/senXARXzO20
#EurekaPrizes @komanderlab.bsky.social @zhongyangan.bsky.social @sylviecallegari.bsky.social @nskirk.bsky.social
Learn more: youtu.be/senXARXzO20
#EurekaPrizes @komanderlab.bsky.social @zhongyangan.bsky.social @sylviecallegari.bsky.social @nskirk.bsky.social
July 31, 2025 at 12:00 AM
PINK1 Parkinson's Disease Research Team @wehi-research.bsky.social is a finalist in the @unsw.edu.au Eureka Prize for Scientific Research.
Learn more: youtu.be/senXARXzO20
#EurekaPrizes @komanderlab.bsky.social @zhongyangan.bsky.social @sylviecallegari.bsky.social @nskirk.bsky.social
Learn more: youtu.be/senXARXzO20
#EurekaPrizes @komanderlab.bsky.social @zhongyangan.bsky.social @sylviecallegari.bsky.social @nskirk.bsky.social
🎉 What an honour to be a finalist in the 2025 Australian Museum Eureka Prizes for Scientific Discovery!! With David Komander, @zhongyangan.bsky.social, Alisa Glukhova and @nskirk.bsky.social.
Excited for the awards ceremony on Sept 3rd!
@eurekaprizes.bsky.social
@wehi-research.bsky.social
Excited for the awards ceremony on Sept 3rd!
@eurekaprizes.bsky.social
@wehi-research.bsky.social
PINK1 Parkinson's Disease Research Team – Finalist, 2025 Eureka Prize for Scientific Research
YouTube video by Australian Museum
www.youtube.com
July 31, 2025 at 12:00 AM
🎉 What an honour to be a finalist in the 2025 Australian Museum Eureka Prizes for Scientific Discovery!! With David Komander, @zhongyangan.bsky.social, Alisa Glukhova and @nskirk.bsky.social.
Excited for the awards ceremony on Sept 3rd!
@eurekaprizes.bsky.social
@wehi-research.bsky.social
Excited for the awards ceremony on Sept 3rd!
@eurekaprizes.bsky.social
@wehi-research.bsky.social
Reposted by Sylvie Callegari
“Why do we sleep” has been a surprisingly hard question. But it looks like the answer may be “ Because we breathe”. Our mitochondria need a break!
It All Comes Down to the Mitochondria
www.science.org
July 30, 2025 at 12:21 PM
“Why do we sleep” has been a surprisingly hard question. But it looks like the answer may be “ Because we breathe”. Our mitochondria need a break!
Reposted by Sylvie Callegari
📣 Calling all ubiquitin aficionados - one week to go before abstract deadline (July 18th)!! Join us on the coast in Lorne, Australia. www.lorneubiquitin.org
#ubiquitin
#ubiquitin
July 11, 2025 at 7:08 AM
📣 Calling all ubiquitin aficionados - one week to go before abstract deadline (July 18th)!! Join us on the coast in Lorne, Australia. www.lorneubiquitin.org
#ubiquitin
#ubiquitin
Reposted by Sylvie Callegari
Australia’s First Ubiquitin Summit is here! 🥳
📍Lorne, Victoria | 🗓️ 20–23 November 2025
Join us on the scenic coastline of Lorne for 4 days of cutting-edge ubiquitin science, featuring a fantastic lineup of international speakers covering all corners of the Ub world—from mechanisms to therapeutics
📍Lorne, Victoria | 🗓️ 20–23 November 2025
Join us on the scenic coastline of Lorne for 4 days of cutting-edge ubiquitin science, featuring a fantastic lineup of international speakers covering all corners of the Ub world—from mechanisms to therapeutics
July 4, 2025 at 1:27 PM
Australia’s First Ubiquitin Summit is here! 🥳
📍Lorne, Victoria | 🗓️ 20–23 November 2025
Join us on the scenic coastline of Lorne for 4 days of cutting-edge ubiquitin science, featuring a fantastic lineup of international speakers covering all corners of the Ub world—from mechanisms to therapeutics
📍Lorne, Victoria | 🗓️ 20–23 November 2025
Join us on the scenic coastline of Lorne for 4 days of cutting-edge ubiquitin science, featuring a fantastic lineup of international speakers covering all corners of the Ub world—from mechanisms to therapeutics
Reposted by Sylvie Callegari
Researchers in Science have developed a tool to silence specific mitochondria-encoded mRNAs in living cells, which could help scientists understand the origins of certain mitochondria-related diseases. scim.ag/3F6Wm5d
Silencing mitochondrial gene expression in living cells
Mitochondria fulfill central functions in metabolism and energy supply. They express their own genome, which encodes key subunits of the oxidative phosphorylation system. However, central mechanisms u...
scim.ag
May 23, 2025 at 7:23 PM
Researchers in Science have developed a tool to silence specific mitochondria-encoded mRNAs in living cells, which could help scientists understand the origins of certain mitochondria-related diseases. scim.ag/3F6Wm5d
Reposted by Sylvie Callegari
Excited that our work revealing the structure and function of the monster stress response silencing factor, the E3 ligase SIFI, is now out! Congratulations to the most amazing team of Zhi Yang, Diane Haakonsen, Michael Heider and Sam Witus!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Molecular basis of SIFI activity in the integrated stress response - Nature
Nature - Molecular basis of SIFI activity in the integrated stress response
www.nature.com
May 7, 2025 at 10:27 AM
Excited that our work revealing the structure and function of the monster stress response silencing factor, the E3 ligase SIFI, is now out! Congratulations to the most amazing team of Zhi Yang, Diane Haakonsen, Michael Heider and Sam Witus!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Stunning photo of Mars on the cover of Science this week! And super proud that our structure of PINK1 stalled at mitochondrial translocases also gets a cover mention 😊. It's now out in print on page 303!
NASA’s Curiosity rover has uncovered a hidden chemical archive of ancient Mars’ atmosphere, which suggests that large amounts of carbon dioxide have been locked into the planet’s crust, according to a new Science study.
Learn more in this week's issue: scim.ag/3Ed9kOl
Learn more in this week's issue: scim.ag/3Ed9kOl
April 18, 2025 at 5:54 AM
Stunning photo of Mars on the cover of Science this week! And super proud that our structure of PINK1 stalled at mitochondrial translocases also gets a cover mention 😊. It's now out in print on page 303!
Reposted by Sylvie Callegari
Excited to share news from the lab out in Nature Communications – A novel PLpro inhibitor improves outcomes in a pre-clinical model of long COVID
www.nature.com/articles/s41...
@wehi-research.bsky.social
www.nature.com/articles/s41...
@wehi-research.bsky.social
A novel PLpro inhibitor improves outcomes in a pre-clinical model of long COVID - Nature Communications
A novel antiviral targeting the SARS-CoV-2 PLpro protease shows strong efficacy in a mouse model, preventing lung pathology and reducing brain dysfunction. The study provides proof-of-principle that P...
www.nature.com
April 8, 2025 at 10:30 PM
Excited to share news from the lab out in Nature Communications – A novel PLpro inhibitor improves outcomes in a pre-clinical model of long COVID
www.nature.com/articles/s41...
@wehi-research.bsky.social
www.nature.com/articles/s41...
@wehi-research.bsky.social
Reposted by Sylvie Callegari
Join us at the beach for the first Australian Ubiquitin summit from 20-23 Nov! 🏄♀️ Check out the amazing speakers here: www.lorneubiquitin.org
And, why not stay a little longer and join the International Symposium on Autophagy from 16-20 Nov, also in beautiful Lorne ☀️. www.isa11.org
See you there!
And, why not stay a little longer and join the International Symposium on Autophagy from 16-20 Nov, also in beautiful Lorne ☀️. www.isa11.org
See you there!
April 7, 2025 at 10:10 AM
Join us at the beach for the first Australian Ubiquitin summit from 20-23 Nov! 🏄♀️ Check out the amazing speakers here: www.lorneubiquitin.org
And, why not stay a little longer and join the International Symposium on Autophagy from 16-20 Nov, also in beautiful Lorne ☀️. www.isa11.org
See you there!
And, why not stay a little longer and join the International Symposium on Autophagy from 16-20 Nov, also in beautiful Lorne ☀️. www.isa11.org
See you there!
Reposted by Sylvie Callegari
Human PINK1 at a TOM-VDADC array
Structure of the Week/Month/Year(?)! 9eih.
Sylvia Callegari...David Komander, Science 13 March 2025.
#coot Fancy mode
Copyright/license-free - i.e. freely reproducible.
Coot 1.1.14 coming soon!
Structure of the Week/Month/Year(?)! 9eih.
Sylvia Callegari...David Komander, Science 13 March 2025.
#coot Fancy mode
Copyright/license-free - i.e. freely reproducible.
Coot 1.1.14 coming soon!
March 15, 2025 at 1:08 AM
Human PINK1 at a TOM-VDADC array
Structure of the Week/Month/Year(?)! 9eih.
Sylvia Callegari...David Komander, Science 13 March 2025.
#coot Fancy mode
Copyright/license-free - i.e. freely reproducible.
Coot 1.1.14 coming soon!
Structure of the Week/Month/Year(?)! 9eih.
Sylvia Callegari...David Komander, Science 13 March 2025.
#coot Fancy mode
Copyright/license-free - i.e. freely reproducible.
Coot 1.1.14 coming soon!
Reposted by Sylvie Callegari
PINK1/Parkin-dependent mitophagy caught in situ by cryo-ET! New preprint www.biorxiv.org/content/10.1... led by @kev-in-situ-et.bsky.social. This has been a long-standing goal of team @mito911.bsky.social within the @asapresearch.parkinsonsroadmap.org initiative.
March 25, 2025 at 6:15 PM
PINK1/Parkin-dependent mitophagy caught in situ by cryo-ET! New preprint www.biorxiv.org/content/10.1... led by @kev-in-situ-et.bsky.social. This has been a long-standing goal of team @mito911.bsky.social within the @asapresearch.parkinsonsroadmap.org initiative.
Reposted by Sylvie Callegari
I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
March 21, 2025 at 2:09 PM
I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
Reposted by Sylvie Callegari
We're on the cover of @science.org this week. Awesome work by @florentwaltz.bsky.social. #CryoET is reaching crazy resolutions inside cells, and this is just the beginning. Fantastic interpretation of the proton motive force by @verenaresch.bsky.social. Always a pleasure making #SciArt with you!🧪🧶🧬🌾
In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion.
Learn more in this week's issue: scim.ag/3FA3Ygq
Learn more in this week's issue: scim.ag/3FA3Ygq
March 20, 2025 at 10:37 PM
We're on the cover of @science.org this week. Awesome work by @florentwaltz.bsky.social. #CryoET is reaching crazy resolutions inside cells, and this is just the beginning. Fantastic interpretation of the proton motive force by @verenaresch.bsky.social. Always a pleasure making #SciArt with you!🧪🧶🧬🌾
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
March 13, 2025 at 7:20 PM
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
Reposted by Sylvie Callegari
Amazing talk by @sylviecallegari.bsky.social so great to see the TOM complex back at Lorne, and capturing substrate in the channel of Tom40 … fabulous
February 12, 2025 at 6:33 AM
Amazing talk by @sylviecallegari.bsky.social so great to see the TOM complex back at Lorne, and capturing substrate in the channel of Tom40 … fabulous