Dan Grabarczyk
@dangrabarczyk.bsky.social
Structural biologist - currently ubiquitin ligases, previously DNA replication and bacterial metabolism
Research Associate - Clausen Lab, IMP Vienna
Research Associate - Clausen Lab, IMP Vienna
Reposted by Dan Grabarczyk
To regulate gene activity, proteasomes - waste disposal machines of cells - must enter the nucleus. IMP Researchers now show how the adaptor protein AKIRIN2 helps ferry this massive complex through the nuclear pore: https://www.nature.com/articles/s41467-026-69162-0
February 11, 2026 at 10:20 AM
To regulate gene activity, proteasomes - waste disposal machines of cells - must enter the nucleus. IMP Researchers now show how the adaptor protein AKIRIN2 helps ferry this massive complex through the nuclear pore: https://www.nature.com/articles/s41467-026-69162-0
Some cool E3 ligase structures here with calmodulin-mediated oligomerisation. Also interesting collection/processing to deal with preferred orientation.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
www.biorxiv.org
February 11, 2026 at 1:50 PM
Some cool E3 ligase structures here with calmodulin-mediated oligomerisation. Also interesting collection/processing to deal with preferred orientation.
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Reposted by Dan Grabarczyk
@lorenzoorts.bsky.social previously worked as a postdoctoral fellow at @impvienna.bsky.social in Austria. She will establish a new lab at IMB to investigate how mRNA translation is activated in the early embryo. Welcome to IMB Laura! 💐
Read more here: www.imb.de/about-imb/ne...
Read more here: www.imb.de/about-imb/ne...
February 6, 2026 at 2:27 PM
@lorenzoorts.bsky.social previously worked as a postdoctoral fellow at @impvienna.bsky.social in Austria. She will establish a new lab at IMB to investigate how mRNA translation is activated in the early embryo. Welcome to IMB Laura! 💐
Read more here: www.imb.de/about-imb/ne...
Read more here: www.imb.de/about-imb/ne...
Reposted by Dan Grabarczyk
After a long (but fun!) postdoc journey, I am very excited to share that I will start my own lab in the Ubiquitin Signalling Division at WEHI. We will study how ubiquitin controls mitochondrial health and its role in neurodegenerative disease. Hiring soon!
Say hello to our incoming lab heads! 👋 Excited to announce Dr Danika Hill, Dr Jacki Heraud-Farlow and Dr Sylvie Callegari will soon be establishing new labs at WEHI.
More info:
www.wehi.edu.au/news/excepti...
bsky.app/profile/sylv...
bsky.app/profile/jack...
bsky.app/profile/prec...
More info:
www.wehi.edu.au/news/excepti...
bsky.app/profile/sylv...
bsky.app/profile/jack...
bsky.app/profile/prec...
February 4, 2026 at 4:26 AM
After a long (but fun!) postdoc journey, I am very excited to share that I will start my own lab in the Ubiquitin Signalling Division at WEHI. We will study how ubiquitin controls mitochondrial health and its role in neurodegenerative disease. Hiring soon!
Reposted by Dan Grabarczyk
When RNA Degradation 🤝 meets 🤝 Protein Degradation! tinyurl.com/E3TDMD In a collaboration of @bartellab.bsky.social and Schulman lab, we show that, in target-directed microRNA degradation (TDMD), 2-RNA-factors recruit an E3 ligase and induce the degradation of not only a protein but also RNA (1/5).
January 6, 2026 at 8:05 AM
When RNA Degradation 🤝 meets 🤝 Protein Degradation! tinyurl.com/E3TDMD In a collaboration of @bartellab.bsky.social and Schulman lab, we show that, in target-directed microRNA degradation (TDMD), 2-RNA-factors recruit an E3 ligase and induce the degradation of not only a protein but also RNA (1/5).
Reposted by Dan Grabarczyk
Vaults. They are cell biology's greatest puzzle! This preprint from Martin Beck's lab shows them docked on ER membranes with a ribosome inside. What on earth is going on there??
#CellBiology #WTFology
www.biorxiv.org/content/10.6...
#CellBiology #WTFology
www.biorxiv.org/content/10.6...
The vault associates with membranes in situ
The eukaryotic vault particle is a giant ribonucleoprotein complex that assembles into an iconic barrel-like cage. Its cellular function has remained elusive despite extensive characterization. Using ...
www.biorxiv.org
December 16, 2025 at 6:35 AM
Vaults. They are cell biology's greatest puzzle! This preprint from Martin Beck's lab shows them docked on ER membranes with a ribosome inside. What on earth is going on there??
#CellBiology #WTFology
www.biorxiv.org/content/10.6...
#CellBiology #WTFology
www.biorxiv.org/content/10.6...
Reposted by Dan Grabarczyk
Reposted by Dan Grabarczyk
SAVE THE DATE!
The EMBO workshop "Ubiquitin and Ubiquitin-like proteins in Health and Disease" will be on September 2026.
Exciting line up of talks and an amazing location!
Organised by @kulathu.bsky.social, @merbllab.bsky.social, @simonapolo.bsky.social, Claudio Joazeiro.
Registration open soon.
The EMBO workshop "Ubiquitin and Ubiquitin-like proteins in Health and Disease" will be on September 2026.
Exciting line up of talks and an amazing location!
Organised by @kulathu.bsky.social, @merbllab.bsky.social, @simonapolo.bsky.social, Claudio Joazeiro.
Registration open soon.
December 8, 2025 at 1:16 PM
SAVE THE DATE!
The EMBO workshop "Ubiquitin and Ubiquitin-like proteins in Health and Disease" will be on September 2026.
Exciting line up of talks and an amazing location!
Organised by @kulathu.bsky.social, @merbllab.bsky.social, @simonapolo.bsky.social, Claudio Joazeiro.
Registration open soon.
The EMBO workshop "Ubiquitin and Ubiquitin-like proteins in Health and Disease" will be on September 2026.
Exciting line up of talks and an amazing location!
Organised by @kulathu.bsky.social, @merbllab.bsky.social, @simonapolo.bsky.social, Claudio Joazeiro.
Registration open soon.
Reposted by Dan Grabarczyk
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
Filament formation and NAD processing by noncanonical human FAM118 sirtuins
Nature Structural & Molecular Biology - Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that...
rdcu.be
November 17, 2025 at 11:37 AM
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
Reposted by Dan Grabarczyk
A new Review discusses how degrons of different types, with an emphasis on high-order structural ones, regulate substrate-E3 interactions and how their dysregulation is linked to human diseases
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Degrons and degradation signals beyond short linear motifs - Nature Chemical Biology
Degrons are degradation signals in target proteins to direct recognition and degradation by the ubiquitin–proteasome system. Wang et al. introduce degrons of different types, with an emphasis on high-...
www.nature.com
November 14, 2025 at 8:51 PM
A new Review discusses how degrons of different types, with an emphasis on high-order structural ones, regulate substrate-E3 interactions and how their dysregulation is linked to human diseases
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Dan Grabarczyk
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
November 7, 2025 at 10:44 AM
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Reposted by Dan Grabarczyk
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
October 31, 2025 at 9:24 AM
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
Reposted by Dan Grabarczyk
Split-site ubiquitination gives ZNFX1 new power in RNA defense @cellcellpress.bsky.social
www.cell.com/molecular-ce...
www.sciencedirect.com/science/arti...
www.cell.com/molecular-ce...
www.sciencedirect.com/science/arti...
Split-site ubiquitination gives ZNFX1 new power in RNA defense
Recent work by Grabarczyk et al. uncovers the molecular mechanism by which ZNFX1,
an interferon-stimulated gene, employs a novel split-site E3 ligase domain structure
to ubiquitinate both protein lysi...
www.cell.com
October 17, 2025 at 4:37 PM
Split-site ubiquitination gives ZNFX1 new power in RNA defense @cellcellpress.bsky.social
www.cell.com/molecular-ce...
www.sciencedirect.com/science/arti...
www.cell.com/molecular-ce...
www.sciencedirect.com/science/arti...
Reposted by Dan Grabarczyk
Elaborate machinery for inserting proteins into the outer membrane of bacteria www.nature.com/articles/d41...
Elaborate machinery for inserting proteins into the outer membrane of bacteria
Bacteria in the gut use an unusual molecular apparatus to insert complex proteins into their outer membrane.
www.nature.com
October 2, 2025 at 9:29 AM
Elaborate machinery for inserting proteins into the outer membrane of bacteria www.nature.com/articles/d41...
Reposted by Dan Grabarczyk
Structure and substrate recognition by the Twin-arginine translocation (Tat) pathway core complex https://www.biorxiv.org/content/10.1101/2025.09.18.677151v1
September 19, 2025 at 3:46 AM
Structure and substrate recognition by the Twin-arginine translocation (Tat) pathway core complex https://www.biorxiv.org/content/10.1101/2025.09.18.677151v1
Thanks a lot to Yiyun Song @natchembio.nature.com for highlighting our ZNFX1 paper. www.nature.com/articles/s41...
Dimerization for activation - Nature Chemical Biology
Nature Chemical Biology - Dimerization for activation
www.nature.com
September 27, 2025 at 1:52 PM
Thanks a lot to Yiyun Song @natchembio.nature.com for highlighting our ZNFX1 paper. www.nature.com/articles/s41...
Reposted by Dan Grabarczyk
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
September 23, 2025 at 5:08 PM
I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪
Reposted by Dan Grabarczyk
I am excited to announce that I will be moving to IMB Mainz next year! The Winter call for the IPP PhD program is now open; if you are interested in maternal #mRNA regulation and #translation in early vertebrate development, please apply! Deadline: 16 October.
More info: www.imb.de/students-pos...
More info: www.imb.de/students-pos...
Laura Lorenzo Orts
IMB Mainz
www.imb.de
September 15, 2025 at 12:13 PM
I am excited to announce that I will be moving to IMB Mainz next year! The Winter call for the IPP PhD program is now open; if you are interested in maternal #mRNA regulation and #translation in early vertebrate development, please apply! Deadline: 16 October.
More info: www.imb.de/students-pos...
More info: www.imb.de/students-pos...
Reposted by Dan Grabarczyk
Pre-print: our description of RZ affiliate ZNFX1 is now online.
On binding viral RNA, ZNFX1 generates parallel Ub currents, coordinating Ub-dependent RNA silencing and aggregate resolution.
Huge effort from first authors Dan Squair and @eilidhrivers.bsky.social
www.biorxiv.org/content/10.1...
On binding viral RNA, ZNFX1 generates parallel Ub currents, coordinating Ub-dependent RNA silencing and aggregate resolution.
Huge effort from first authors Dan Squair and @eilidhrivers.bsky.social
www.biorxiv.org/content/10.1...
ZNFX1 uses two-component ubiquitin circuitry to quarantine viral RNA
The detection of viral RNA inside cells triggers a diverse range of antiviral responses, including global translation inhibition, interferon secretion and RNA sequestration. Mutations in the gene ZNFX...
www.biorxiv.org
September 8, 2025 at 1:53 PM
Pre-print: our description of RZ affiliate ZNFX1 is now online.
On binding viral RNA, ZNFX1 generates parallel Ub currents, coordinating Ub-dependent RNA silencing and aggregate resolution.
Huge effort from first authors Dan Squair and @eilidhrivers.bsky.social
www.biorxiv.org/content/10.1...
On binding viral RNA, ZNFX1 generates parallel Ub currents, coordinating Ub-dependent RNA silencing and aggregate resolution.
Huge effort from first authors Dan Squair and @eilidhrivers.bsky.social
www.biorxiv.org/content/10.1...
Reposted by Dan Grabarczyk
Researchers around @clausenlab.bsky.social & @dangrabarczyk.bsky.social have unveiled the three-dimensional structure of UBR4, a giant protein complex that safeguards cells by targeting defective proteins for destruction—offering fresh insights into health & disease. www.science.org/doi/10.1126/...
Inside the cell’s recycling hub: unveiling the architecture of UBR4
Researchers from the lab of Tim Clausen and collaborators have unveiled the three-dimensional structure of UBR4, a giant protein complex that safeguards cells by targeting defective proteins for destr...
www.imp.ac.at
August 28, 2025 at 6:28 PM
Researchers around @clausenlab.bsky.social & @dangrabarczyk.bsky.social have unveiled the three-dimensional structure of UBR4, a giant protein complex that safeguards cells by targeting defective proteins for destruction—offering fresh insights into health & disease. www.science.org/doi/10.1126/...
Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...
August 28, 2025 at 6:54 PM
Glad to share the final version of our story about the UBR4 complex, an E4 ligase protein quality control hub @science.org. Now with more cryo-EM structures and a deeper dive into substrate recognition, especially escaped mitochondrial proteins @clausenlab.bsky.social www.science.org/doi/10.1126/...
Reposted by Dan Grabarczyk
Scientists uncovered how the immune protein ZNFX1 enables cells to walk the fine line between fighting infection & avoiding autoimmune damage. ZNFX1 can ubiquitinate & compact host & viral RNA into molecular condensates, extending ubiquitin biology to RNA itself. More: www.imp.ac.at/news/article...
ZNFX1 compacts and tags RNA to keep immunity in check
Tim Clausen’s lab at the IMP and collaborators have uncovered how the ancient immune protein ZNFX1 enables cells to walk the fine line between fighting infection and avoiding autoimmune damage. The te...
www.imp.ac.at
August 28, 2025 at 7:33 AM
Scientists uncovered how the immune protein ZNFX1 enables cells to walk the fine line between fighting infection & avoiding autoimmune damage. ZNFX1 can ubiquitinate & compact host & viral RNA into molecular condensates, extending ubiquitin biology to RNA itself. More: www.imp.ac.at/news/article...
Happy to share our work on the structure and function of the unusual E3 ligase ZNFX1 @cp-cell.bsky.social. It uses a nucleic acid-activated transthiolation mechanism, ubiquitinating and clustering RNA to protect cells in an immune response. @clausenlab.bsky.social
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles
Grabarczyk et al. show the structure and mechanism of a non-canonical ubiquitin ligase,
which is activated through nucleic-acid-induced oligomerization and is critical for
cell survival during immune ...
www.cell.com
August 27, 2025 at 7:04 PM
Happy to share our work on the structure and function of the unusual E3 ligase ZNFX1 @cp-cell.bsky.social. It uses a nucleic acid-activated transthiolation mechanism, ubiquitinating and clustering RNA to protect cells in an immune response. @clausenlab.bsky.social
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Reposted by Dan Grabarczyk
Opening for a biochemist to help us in the characterisation of novel ubiquitin-based antivirals. Diverse backgrounds welcomed; a keen sense of adventure is a must 😁
Details and criteria are below; please get in touch with any questions.
www.jobs.gla.ac.uk/job/research...
Details and criteria are below; please get in touch with any questions.
www.jobs.gla.ac.uk/job/research...
Research Assistant/Associate
Research Assistant/Associate COLLEGE OF MVLSSchool of Infection & ImmunityResearch and TeachingGRADE 6/7 Job PurposeThe post holder will join the School of Infection & Immunity, MRC-Univers...
www.jobs.gla.ac.uk
August 25, 2025 at 12:34 PM
Opening for a biochemist to help us in the characterisation of novel ubiquitin-based antivirals. Diverse backgrounds welcomed; a keen sense of adventure is a must 😁
Details and criteria are below; please get in touch with any questions.
www.jobs.gla.ac.uk/job/research...
Details and criteria are below; please get in touch with any questions.
www.jobs.gla.ac.uk/job/research...