Reposted by Lander Lab
Helical Reconstruction of Amyloids in cryoSPARC?
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
October 5, 2025 at 4:16 AM
Helical Reconstruction of Amyloids in cryoSPARC?
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
We present guidelines, limitations & future perspectives for processing amyloids in cryoSPARC @structurabio.bsky.social.
Great collaboration of the Kelly Lab and @landerlab.bsky.social #cryoEM #amyloid www.biorxiv.org/content/10.1...
So happy to see these developments! Particularly the structure of the 29 kDa heterodimer of hemoglobin! Well done Oli & co! Of course can’t overlook the importance of sample prep and data collection - credit to Mark Herzik and Mengyu Wu
This one is a bit of a departure from the usual and definitely a work in progress!
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.
Read on for details... 1/x
September 13, 2025 at 3:36 AM
So happy to see these developments! Particularly the structure of the 29 kDa heterodimer of hemoglobin! Well done Oli & co! Of course can’t overlook the importance of sample prep and data collection - credit to Mark Herzik and Mengyu Wu
Reposted by Lander Lab
Check out our new preprint from the @landerlab.bsky.social, Stagg-Lab and Cianfrocco-Lab: CryoSift - An accessible and automated CNN-driven tool for cryo-EM 2D class selection www.biorxiv.org/content/10.1...
Happy testing: cryosift.org #cryoEM #CNN
Happy testing: cryosift.org #cryoEM #CNN
August 1, 2025 at 3:20 PM
Check out our new preprint from the @landerlab.bsky.social, Stagg-Lab and Cianfrocco-Lab: CryoSift - An accessible and automated CNN-driven tool for cryo-EM 2D class selection www.biorxiv.org/content/10.1...
Happy testing: cryosift.org #cryoEM #CNN
Happy testing: cryosift.org #cryoEM #CNN
Reposted by Lander Lab
Thanks for highlighting this great story! @landerlab.bsky.social and I wrote a PREreview about the preprint: prereview.org/reviews/1581...
PREreview of “High-resolution cryo-EM structures of small protein–ligand complexes near the theoretical size limit”
Authored by Jan-Hannes Schäfer and Gabriel C. Lander
prereview.org
August 14, 2025 at 2:51 AM
Thanks for highlighting this great story! @landerlab.bsky.social and I wrote a PREreview about the preprint: prereview.org/reviews/1581...
Reposted by Lander Lab
Exited to share my first PostDoc contribution! Like Magellan explored the globe, you can explore your cryoEM data with Magellon. Congrats to all involved. Scott Stagg Lab & Michael Cianfrocco Lab @landerlab.bsky.social www.biorxiv.org/content/10.1... 🌎❄️🔬
June 23, 2025 at 11:28 PM
Exited to share my first PostDoc contribution! Like Magellan explored the globe, you can explore your cryoEM data with Magellon. Congrats to all involved. Scott Stagg Lab & Michael Cianfrocco Lab @landerlab.bsky.social www.biorxiv.org/content/10.1... 🌎❄️🔬
Reposted by Lander Lab
In this feature, Gabe Lander (@landerlab.bsky.social) and Adrian Koh discuss how they are working to improve cryo-EM technology >>> www.biotechniques.com/proteomics/s...
@scripps.edu 🧪
@scripps.edu 🧪
Seeking structure: an insight into cryo-EM, structural biology and drug development
Gabe Lander and Adrian Koh talk all things cryo-EM and its pivotal role in structural biology research in this feature.
www.biotechniques.com
May 8, 2025 at 8:12 AM
In this feature, Gabe Lander (@landerlab.bsky.social) and Adrian Koh discuss how they are working to improve cryo-EM technology >>> www.biotechniques.com/proteomics/s...
@scripps.edu 🧪
@scripps.edu 🧪
Reposted by Lander Lab
🚨New preprint!🚨 #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
Surface morphometrics reveals local membrane thickness variation in organellar subcompartments https://www.biorxiv.org/content/10.1101/2025.04.30.651574v1
May 1, 2025 at 7:42 PM
🚨New preprint!🚨 #teamtomo
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
We expanded Surface Morphometrics to quantify membrane thickness from cryo-ET—revealing local variation across organelles.
Led by the lab’s first grad student, @mmedina300kv.bsky.social (defending Monday! 🍾) w/ @attychang.bsky.social @hamid13r.bsky.social & @tomo.science
Our latest review is out in COSB! Led by Zeba Rizvi @zrizvi.bsky.social, we explore how cryo-EM has brought new insights to Cullin RING ligases - from conformations to allosteric control, cryo-EM has massively improved our understanding of these dynamic molecular machines. tinyurl.com/y4sza8dh
May 1, 2025 at 4:39 PM
Our latest review is out in COSB! Led by Zeba Rizvi @zrizvi.bsky.social, we explore how cryo-EM has brought new insights to Cullin RING ligases - from conformations to allosteric control, cryo-EM has massively improved our understanding of these dynamic molecular machines. tinyurl.com/y4sza8dh
Social media has been great in helping us identify contaminants or cofactors in the past - Any ideas what this complex might be in our HEK293 FLAG pulldown? (We should put together a repository of common contaminants in different cell types)
First post here- Hello Bluesky!
Quick science query: These 2D classes are from negative stain of
a FLAG pulldown (HEK cell eluate). Does this look like a real complex or a common contaminant? Would live to hear from anyone who’s worked on similar prep!
#negativestainEM #pulldown #HEKcells
Quick science query: These 2D classes are from negative stain of
a FLAG pulldown (HEK cell eluate). Does this look like a real complex or a common contaminant? Would live to hear from anyone who’s worked on similar prep!
#negativestainEM #pulldown #HEKcells
April 21, 2025 at 5:26 PM
Social media has been great in helping us identify contaminants or cofactors in the past - Any ideas what this complex might be in our HEK293 FLAG pulldown? (We should put together a repository of common contaminants in different cell types)
Reposted by Lander Lab
Finally recovered from 2 weeks of #cryoEM What a blast! After 3 times TA-ing for CSHL, I can’t begin to describe the joy it has brought me to teach and learn at this amazing course. Thanks to @jencash.bsky.social @landerlab.bsky.social @matthijnvos.bsky.social et al. for bringing this to life!
March 23, 2025 at 8:24 PM
Finally recovered from 2 weeks of #cryoEM What a blast! After 3 times TA-ing for CSHL, I can’t begin to describe the joy it has brought me to teach and learn at this amazing course. Thanks to @jencash.bsky.social @landerlab.bsky.social @matthijnvos.bsky.social et al. for bringing this to life!
Reposted by Lander Lab
A pie graph worth keeping in mind as the NIH budget plummets jamanetwork.com/journals/jam... for 356 new FDA drugs approved
March 23, 2025 at 4:17 PM
A pie graph worth keeping in mind as the NIH budget plummets jamanetwork.com/journals/jam... for 356 new FDA drugs approved
Reposted by Lander Lab
Read of the week. Is this future of freezing cryo-EM grids - Foam Film Vitrification without blotting? Congrats to Russo and Löwe labs!
Foam film vitrification for cryo-EM https://www.biorxiv.org/content/10.1101/2025.03.10.642366v1
March 22, 2025 at 1:46 AM
Read of the week. Is this future of freezing cryo-EM grids - Foam Film Vitrification without blotting? Congrats to Russo and Löwe labs!
Big congrats to Dr. Kelly Chen on completing her PhD! She wrangled human ClpXP into submission with CryoEM, and now she's off to tackle new challenges at Genentech. We couldn’t be more proud of all she’s accomplished, and we all wish her all the best in this next chapter of her scientific journey.
March 21, 2025 at 11:53 PM
Big congrats to Dr. Kelly Chen on completing her PhD! She wrangled human ClpXP into submission with CryoEM, and now she's off to tackle new challenges at Genentech. We couldn’t be more proud of all she’s accomplished, and we all wish her all the best in this next chapter of her scientific journey.
That’s a wrap on the 2025 Cold Spring Harbor CryoEM course! Huge thanks to the amazing students for your enthusiasm, the TAs for your dedication, and the invited lecturers for sharing your expertise. Keep pushing the boundaries of single particle EM - excited to see what you all accomplish next!
March 19, 2025 at 5:55 PM
That’s a wrap on the 2025 Cold Spring Harbor CryoEM course! Huge thanks to the amazing students for your enthusiasm, the TAs for your dedication, and the invited lecturers for sharing your expertise. Keep pushing the boundaries of single particle EM - excited to see what you all accomplish next!
Congrats to Group 1, winners of the 2025 CSHL #CryoEM course! They reconstructed a 1.9 A structure from 100 images, the built and refined an outstanding atomic model with great validation metrics. They are the recipients of the new Cryo Champion Chalice, adorned with $2,467 worth of used autogrids!
March 19, 2025 at 2:24 AM
Congrats to Group 1, winners of the 2025 CSHL #CryoEM course! They reconstructed a 1.9 A structure from 100 images, the built and refined an outstanding atomic model with great validation metrics. They are the recipients of the new Cryo Champion Chalice, adorned with $2,467 worth of used autogrids!
CSHL 2025 #CryoEM course update - final hour of the competition and the teams are refining their atomic models. Amazing what these students have done with 100 micrographs in just a few hours!
March 18, 2025 at 8:25 PM
CSHL 2025 #CryoEM course update - final hour of the competition and the teams are refining their atomic models. Amazing what these students have done with 100 micrographs in just a few hours!
It’s the last full day of the CSHL #CryoEM course! Last leg of the competition - solve the structure of a mystery protein and generate an atomic model with a complete Table 1 (sequence not provided). Students are learning that ChatGPT isn’t much help when trying to identify symmetry of a structure…
March 18, 2025 at 3:47 PM
It’s the last full day of the CSHL #CryoEM course! Last leg of the competition - solve the structure of a mystery protein and generate an atomic model with a complete Table 1 (sequence not provided). Students are learning that ChatGPT isn’t much help when trying to identify symmetry of a structure…
Brace yourselves, Matthijn Vos @matthijnvos.bsky.social has arrived! EM expert & head of the NanoImaging Core at the Institut Pasteur, he's known for his razor-sharp critiques and brutal honesty. But beneath the grumpiness? A brilliant scientist and an invaluable colleague. Welcome, Matthijn!
March 18, 2025 at 1:38 PM
Brace yourselves, Matthijn Vos @matthijnvos.bsky.social has arrived! EM expert & head of the NanoImaging Core at the Institut Pasteur, he's known for his razor-sharp critiques and brutal honesty. But beneath the grumpiness? A brilliant scientist and an invaluable colleague. Welcome, Matthijn!
Happy St. Patrick’s Day from the CSHL #cryoEM course! Data collection day for the final competition, but it looks like some mischievous leprechauns got into the microscope and messed up the autoloader… A tough but valuable lesson for the students: sometimes your resolution is limited by bad luck…
March 17, 2025 at 7:35 PM
Happy St. Patrick’s Day from the CSHL #cryoEM course! Data collection day for the final competition, but it looks like some mischievous leprechauns got into the microscope and messed up the autoloader… A tough but valuable lesson for the students: sometimes your resolution is limited by bad luck…
Day 11 of the CSHL #CryoEM course: depositing data and new developments at the EMDB with Jack Turner, @jencash.bsky.social showed how to make graphene oxide grids, and learned how to make local resolution maps. Competitive data collection starts tomorrow!
March 17, 2025 at 2:50 AM
Day 11 of the CSHL #CryoEM course: depositing data and new developments at the EMDB with Jack Turner, @jencash.bsky.social showed how to make graphene oxide grids, and learned how to make local resolution maps. Competitive data collection starts tomorrow!
We’ve seen some good grids and some bad at the CSHL #CryoEM course, but I think we all agree that @miroastore.bsky.social should be awarded the 2025 “worst grid” award. But for someone who never held a pipette prior to this course he also deserves the “most improved” award - keep it up Miro!
March 16, 2025 at 3:48 AM
We’ve seen some good grids and some bad at the CSHL #CryoEM course, but I think we all agree that @miroastore.bsky.social should be awarded the 2025 “worst grid” award. But for someone who never held a pipette prior to this course he also deserves the “most improved” award - keep it up Miro!
Saturday night and the lab is in full swing as the participants of the CSHL #CryoEM course prepare for the final competition - solve the structure of a mystery protein in less than 4 hours.
March 16, 2025 at 12:52 AM
Saturday night and the lab is in full swing as the participants of the CSHL #CryoEM course prepare for the final competition - solve the structure of a mystery protein in less than 4 hours.
Outstanding lecture on EM validation by Oli Clarke @olibclarke.bsky.social this morning at the CSHL #cryoEM course.
March 14, 2025 at 3:43 PM
Outstanding lecture on EM validation by Oli Clarke @olibclarke.bsky.social this morning at the CSHL #cryoEM course.
Today at the CSHL #CryoEM course: we began with an excellent overview of atomic model validation by Christopher Williams, then an in-depth modeling tutorial by @jencash.bsky.social, a masking and local refinement tutorial by Kelli H, and tonight the students begin flying solo on the Krios!
March 14, 2025 at 12:25 AM
Today at the CSHL #CryoEM course: we began with an excellent overview of atomic model validation by Christopher Williams, then an in-depth modeling tutorial by @jencash.bsky.social, a masking and local refinement tutorial by Kelli H, and tonight the students begin flying solo on the Krios!
Reposted by Lander Lab
Sad news about Karen Davies' passing.
Karen was an inspiration in bioenergetics and cryoET. Her pioneering postdoctoral work on ATP synthase and respiratory supercomplexes marked turning points in the field. I created Scholar profile to acknowledge her contribution
scholar.google.com/citations?hl...
Karen was an inspiration in bioenergetics and cryoET. Her pioneering postdoctoral work on ATP synthase and respiratory supercomplexes marked turning points in the field. I created Scholar profile to acknowledge her contribution
scholar.google.com/citations?hl...
Karen Davies
Formerly of Electron Bio-Imaging Centre, Diamond Light Source, United Kingdom - Cited by 2,887 - cryo-EM - tomography - method development - bioenergetics
scholar.google.com
March 12, 2025 at 8:33 PM
Sad news about Karen Davies' passing.
Karen was an inspiration in bioenergetics and cryoET. Her pioneering postdoctoral work on ATP synthase and respiratory supercomplexes marked turning points in the field. I created Scholar profile to acknowledge her contribution
scholar.google.com/citations?hl...
Karen was an inspiration in bioenergetics and cryoET. Her pioneering postdoctoral work on ATP synthase and respiratory supercomplexes marked turning points in the field. I created Scholar profile to acknowledge her contribution
scholar.google.com/citations?hl...