Our latest review is out in COSB! Led by Zeba Rizvi @zrizvi.bsky.social, we explore how cryo-EM has brought new insights to Cullin RING ligases - from conformations to allosteric control, cryo-EM has massively improved our understanding of these dynamic molecular machines. tinyurl.com/y4sza8dh
May 1, 2025 at 4:39 PM
Our latest review is out in COSB! Led by Zeba Rizvi @zrizvi.bsky.social, we explore how cryo-EM has brought new insights to Cullin RING ligases - from conformations to allosteric control, cryo-EM has massively improved our understanding of these dynamic molecular machines. tinyurl.com/y4sza8dh
Big congrats to Dr. Kelly Chen on completing her PhD! She wrangled human ClpXP into submission with CryoEM, and now she's off to tackle new challenges at Genentech. We couldn’t be more proud of all she’s accomplished, and we all wish her all the best in this next chapter of her scientific journey.
March 21, 2025 at 11:53 PM
Big congrats to Dr. Kelly Chen on completing her PhD! She wrangled human ClpXP into submission with CryoEM, and now she's off to tackle new challenges at Genentech. We couldn’t be more proud of all she’s accomplished, and we all wish her all the best in this next chapter of her scientific journey.
That’s a wrap on the 2025 Cold Spring Harbor CryoEM course! Huge thanks to the amazing students for your enthusiasm, the TAs for your dedication, and the invited lecturers for sharing your expertise. Keep pushing the boundaries of single particle EM - excited to see what you all accomplish next!
March 19, 2025 at 5:55 PM
That’s a wrap on the 2025 Cold Spring Harbor CryoEM course! Huge thanks to the amazing students for your enthusiasm, the TAs for your dedication, and the invited lecturers for sharing your expertise. Keep pushing the boundaries of single particle EM - excited to see what you all accomplish next!
Congrats to Group 1, winners of the 2025 CSHL #CryoEM course! They reconstructed a 1.9 A structure from 100 images, the built and refined an outstanding atomic model with great validation metrics. They are the recipients of the new Cryo Champion Chalice, adorned with $2,467 worth of used autogrids!
March 19, 2025 at 2:24 AM
Congrats to Group 1, winners of the 2025 CSHL #CryoEM course! They reconstructed a 1.9 A structure from 100 images, the built and refined an outstanding atomic model with great validation metrics. They are the recipients of the new Cryo Champion Chalice, adorned with $2,467 worth of used autogrids!
CSHL 2025 #CryoEM course update - final hour of the competition and the teams are refining their atomic models. Amazing what these students have done with 100 micrographs in just a few hours!
March 18, 2025 at 8:25 PM
CSHL 2025 #CryoEM course update - final hour of the competition and the teams are refining their atomic models. Amazing what these students have done with 100 micrographs in just a few hours!
It’s the last full day of the CSHL #CryoEM course! Last leg of the competition - solve the structure of a mystery protein and generate an atomic model with a complete Table 1 (sequence not provided). Students are learning that ChatGPT isn’t much help when trying to identify symmetry of a structure…
March 18, 2025 at 3:47 PM
It’s the last full day of the CSHL #CryoEM course! Last leg of the competition - solve the structure of a mystery protein and generate an atomic model with a complete Table 1 (sequence not provided). Students are learning that ChatGPT isn’t much help when trying to identify symmetry of a structure…
Brace yourselves, Matthijn Vos @matthijnvos.bsky.social has arrived! EM expert & head of the NanoImaging Core at the Institut Pasteur, he's known for his razor-sharp critiques and brutal honesty. But beneath the grumpiness? A brilliant scientist and an invaluable colleague. Welcome, Matthijn!
March 18, 2025 at 1:38 PM
Brace yourselves, Matthijn Vos @matthijnvos.bsky.social has arrived! EM expert & head of the NanoImaging Core at the Institut Pasteur, he's known for his razor-sharp critiques and brutal honesty. But beneath the grumpiness? A brilliant scientist and an invaluable colleague. Welcome, Matthijn!
Happy St. Patrick’s Day from the CSHL #cryoEM course! Data collection day for the final competition, but it looks like some mischievous leprechauns got into the microscope and messed up the autoloader… A tough but valuable lesson for the students: sometimes your resolution is limited by bad luck…
March 17, 2025 at 7:35 PM
Happy St. Patrick’s Day from the CSHL #cryoEM course! Data collection day for the final competition, but it looks like some mischievous leprechauns got into the microscope and messed up the autoloader… A tough but valuable lesson for the students: sometimes your resolution is limited by bad luck…
Day 11 of the CSHL #CryoEM course: depositing data and new developments at the EMDB with Jack Turner, @jencash.bsky.social showed how to make graphene oxide grids, and learned how to make local resolution maps. Competitive data collection starts tomorrow!
March 17, 2025 at 2:50 AM
Day 11 of the CSHL #CryoEM course: depositing data and new developments at the EMDB with Jack Turner, @jencash.bsky.social showed how to make graphene oxide grids, and learned how to make local resolution maps. Competitive data collection starts tomorrow!
We’ve seen some good grids and some bad at the CSHL #CryoEM course, but I think we all agree that @miroastore.bsky.social should be awarded the 2025 “worst grid” award. But for someone who never held a pipette prior to this course he also deserves the “most improved” award - keep it up Miro!
March 16, 2025 at 3:48 AM
We’ve seen some good grids and some bad at the CSHL #CryoEM course, but I think we all agree that @miroastore.bsky.social should be awarded the 2025 “worst grid” award. But for someone who never held a pipette prior to this course he also deserves the “most improved” award - keep it up Miro!
Saturday night and the lab is in full swing as the participants of the CSHL #CryoEM course prepare for the final competition - solve the structure of a mystery protein in less than 4 hours.
March 16, 2025 at 12:52 AM
Saturday night and the lab is in full swing as the participants of the CSHL #CryoEM course prepare for the final competition - solve the structure of a mystery protein in less than 4 hours.
Outstanding lecture on EM validation by Oli Clarke @olibclarke.bsky.social this morning at the CSHL #cryoEM course.
March 14, 2025 at 3:43 PM
Outstanding lecture on EM validation by Oli Clarke @olibclarke.bsky.social this morning at the CSHL #cryoEM course.
Today at the CSHL #CryoEM course: we began with an excellent overview of atomic model validation by Christopher Williams, then an in-depth modeling tutorial by @jencash.bsky.social, a masking and local refinement tutorial by Kelli H, and tonight the students begin flying solo on the Krios!
March 14, 2025 at 12:25 AM
Today at the CSHL #CryoEM course: we began with an excellent overview of atomic model validation by Christopher Williams, then an in-depth modeling tutorial by @jencash.bsky.social, a masking and local refinement tutorial by Kelli H, and tonight the students begin flying solo on the Krios!
Our first competition at the CSHL #CryoEM course - each student tries to make a perfect grid of ryanodine receptors. We’ll be checking atlases after dinner… who will be tonight’s big winner? Thanks to @olibclarke.bsky.social for providing sample and gold grids!
March 12, 2025 at 9:45 PM
Our first competition at the CSHL #CryoEM course - each student tries to make a perfect grid of ryanodine receptors. We’ll be checking atlases after dinner… who will be tonight’s big winner? Thanks to @olibclarke.bsky.social for providing sample and gold grids!
Day 6 of the CSHL #CryoEM course: field trip to the city! Visited the New York Structural Biology Center, where Ed Eng @edwardteng.bsky.social introduced everyone to the EM sample prep and collection resources available at nccat.nysbc.org. Then a tour of the new technologies being developed there!
March 11, 2025 at 7:07 PM
Day 6 of the CSHL #CryoEM course: field trip to the city! Visited the New York Structural Biology Center, where Ed Eng @edwardteng.bsky.social introduced everyone to the EM sample prep and collection resources available at nccat.nysbc.org. Then a tour of the new technologies being developed there!
Day 5 of the CSHL #cryoEM course: clipping and loading autogrids. Thanks to SubAngstrom for supplying us with rings and clips for the course! Kelli H also gave an amazing tutorial on 3D mask creation, which we put to use for a signal subtraction tutorial run tonight by Katerina Naydenova.
March 11, 2025 at 3:34 AM
Day 5 of the CSHL #cryoEM course: clipping and loading autogrids. Thanks to SubAngstrom for supplying us with rings and clips for the course! Kelli H also gave an amazing tutorial on 3D mask creation, which we put to use for a signal subtraction tutorial run tonight by Katerina Naydenova.
Day 4 of the CSHL #CryoEM course - A great lecture on advanced processing strategies from Justin Kollman, then more hands-on training! Freezing cryo samples with the Leica GP2, TFS Vitrobot, and manual plunger; preparing and checking negative stain grids; and checking alignments on the Krios.
March 9, 2025 at 7:04 PM
Day 4 of the CSHL #CryoEM course - A great lecture on advanced processing strategies from Justin Kollman, then more hands-on training! Freezing cryo samples with the Leica GP2, TFS Vitrobot, and manual plunger; preparing and checking negative stain grids; and checking alignments on the Krios.
Today at the CSHL #CryoEM course Lauren @lalexandrescu.bsky.social showed everyone how to make their own Quantifoil-style holey cryo-em grids! Thanks to @johnrubinstein.bsky.social for introducing us to this technique - I predict it will be adopted by many other labs!
March 8, 2025 at 1:08 AM
Today at the CSHL #CryoEM course Lauren @lalexandrescu.bsky.social showed everyone how to make their own Quantifoil-style holey cryo-em grids! Thanks to @johnrubinstein.bsky.social for introducing us to this technique - I predict it will be adopted by many other labs!
We couldn't get out to #StandUpForScience with everyone else, but lots of great science discussed today at the CSHL #CryoEM course - intro to electron microscope optics with Alex de Marco, Fourier transforms & CTF with Tim Grant, and direct detectors with Katerina Naydenova.
March 7, 2025 at 10:04 PM
We couldn't get out to #StandUpForScience with everyone else, but lots of great science discussed today at the CSHL #CryoEM course - intro to electron microscope optics with Alex de Marco, Fourier transforms & CTF with Tim Grant, and direct detectors with Katerina Naydenova.
BTW welcome to Bluesky, Yifan Cheng @cryosp.bsky.social Professor at UCSF, HHMI investigator, #CryoEM pioneer, and aggregator of fish, chicken, and cannolis on a single plate.
March 7, 2025 at 3:09 AM
BTW welcome to Bluesky, Yifan Cheng @cryosp.bsky.social Professor at UCSF, HHMI investigator, #CryoEM pioneer, and aggregator of fish, chicken, and cannolis on a single plate.
Amazing Day 1 of the CSHL #CryoEM Course! Yifan Cheng @cryosp.bsky.social kicked things off with a historical perspective and discussing dynamic allostery, Kelli Hvorecny gave a workflow overview, Lauren Anderson introduced ChimeraX @www.cgl.ucsf.edu, and Katerina Naydenova ran a RELION tutorial.
March 7, 2025 at 2:49 AM
Amazing Day 1 of the CSHL #CryoEM Course! Yifan Cheng @cryosp.bsky.social kicked things off with a historical perspective and discussing dynamic allostery, Kelli Hvorecny gave a workflow overview, Lauren Anderson introduced ChimeraX @www.cgl.ucsf.edu, and Katerina Naydenova ran a RELION tutorial.
Welcome to the participants of the 2025 #CryoEM course at Cold Spring Harbor Lab @cshlaboratory.bsky.social ! It's going to be a great 2 weeks immersed in all aspects of single particle cryoEM methods.
March 6, 2025 at 4:47 PM
Welcome to the participants of the 2025 #CryoEM course at Cold Spring Harbor Lab @cshlaboratory.bsky.social ! It's going to be a great 2 weeks immersed in all aspects of single particle cryoEM methods.