Adrià Sogues
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adriasogues.bsky.social
Adrià Sogues
@adriasogues.bsky.social
Spores, Bacterial S-layers, Cell Envelope & Cell Division. Structural Microbiologist 💎❄️🔬 at Institut Pasteur (Paris). Formerly at VIB-VUB in Brussels (EMBO & MSCA fellow) 🇫🇷🇧🇪🇪🇸
Pinned
Happy to see the final version out! Cryo-EM structure of the PS2 S-layer in Corynebacterium 🔬! We show it provides mechanical support to the cell wall and assembles at the poles in coordination with PG. It can also be used for covalent surface display.

www.pnas.org/doi/10.1073/...

#microsky
Cryo-EM structure and polar assembly of the PS2 S-layer of Corynebacterium glutamicum | PNAS
The polar-growing Corynebacteriales have a complex cell envelope architecture characterized by the presence of a specialized outer membrane compose...
www.pnas.org
S-layer, a (re)blooming field and a fascinating world for both microbiologists and structural biologists. A very complete and up-to-date review of S-layer functions, structure, and biogenesis. Congrats to all the authors!
November 13, 2025 at 9:20 PM
Reposted by Adrià Sogues
🦠🔬🤖🧑‍💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training

📜 www.biorxiv.org/content/10.1...
October 22, 2025 at 11:10 AM
The line-up I would dream of 🤩
We’re excited to announce the incredible speaker line-up for the Prokaryotic Cell Biology Conference! Join the leading researchers as they explore the latest advances in Nanoimaging, Cell Growth and Cellular Structures.
Join our speakers & register today! ➡️https://bit.ly/47rtkbw
#ProkaryoticCellBio
October 18, 2025 at 1:44 PM
Reposted by Adrià Sogues
Cryo-Flow : when science freezes and flows ❄️

After turning her thesis on tuberculosis into a rap, Yaëlle Wormser aka Lady Pipette, doctoral student at the Institut Pasteur, is back with a new track — Cryo-Flow — a song that turns Cryo-Electron Microscopy (Cryo-EM) into rhythm & rhyme.

@pasteur.fr
Cryo-Flow : when science freezes and flows ❄️
YouTube video by Institut Pasteur EDUCATION
youtube.com
October 15, 2025 at 10:02 AM
Reposted by Adrià Sogues
CRISPRi on #Bdellovibrio to study essential genes 🤗🤩 #MicroSky
🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference
Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.
academic.oup.com
September 18, 2025 at 8:54 AM
A new chapter begins! Happy to be back at the @pasteur.fr , a place that feels like home. Excited for the journey ahead and all the science to come!
September 18, 2025 at 8:52 AM
Reposted by Adrià Sogues
🎓 Applications for Institut Pasteur's PhD programs open Sept 1-Oct 20, 2025 for October 2026 entry.

Apply to up to 4 projects across 6 programs: PPU, PPU-IMAGINE @institutimagine.bsky.social, PPU-@ox.ac.uk, PPU-EID @upcite.bsky.social & more 🧬

Register now ✍️ www.pasteur.fr/en/education...
September 1, 2025 at 9:44 AM
Many spores from the Firmicutes group are decorated with hair-like structures. Since the 60s, they remained a mystery. @remaut-lab.bsky.social named these fibres ENdospore Appendages (ENAs). Using Cryo-ID, we report the identity, structure and function of F-ENA.
www.nature.com/articles/s41...
Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes - Nature Communications
B. thuringiensis spores contain uncharacterized protein filaments that extend from the surface of the exosporium. Here, the authors show that these filaments feature conserved β-barrel neck domains an...
www.nature.com
August 17, 2025 at 5:52 AM
Reposted by Adrià Sogues
Thrilled to see our work published in @embojournal.org
#asgard #archaea #cytoskeleton #tubulin #FtsZ
We sincerely appreciate the constructive peer review and the reviewers’ thoughtful, supportive feedback, which greatly strengthened our work.
Insights into the origins of the #tubulin / #FtsZ superfamily: @syncellbiolab.bsky.social, Pananghat Gayathri et al show that two #Asgard archaeal paralogs OdinFtsZ1 and OdinFtsZ2 form distinct filamental structures, and employ different modes of membrane tethering
www.embopress.org/doi/full/10....
August 8, 2025 at 11:49 AM
Reposted by Adrià Sogues
Excited to share our newly book chapter on using #miniTurbo proximity labeling in #bacteria
We wrote it as a hands-on guide for anyone looking to get started with this approach.
Congrats to @coralietesseur.bsky.social and Ophélie Remy 👏 @lalouxlab.bsky.social
link.springer.com/protocol/10....
Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria
Protein–protein interactions play a crucial role in many bacterial cell processes. miniTurbo-based proximity labeling has emerged as a powerful tool for the identification of in vivo protein net...
link.springer.com
July 14, 2025 at 7:28 AM
Happy to see the final version out! Cryo-EM structure of the PS2 S-layer in Corynebacterium 🔬! We show it provides mechanical support to the cell wall and assembles at the poles in coordination with PG. It can also be used for covalent surface display.

www.pnas.org/doi/10.1073/...

#microsky
Cryo-EM structure and polar assembly of the PS2 S-layer of Corynebacterium glutamicum | PNAS
The polar-growing Corynebacteriales have a complex cell envelope architecture characterized by the presence of a specialized outer membrane compose...
www.pnas.org
July 30, 2025 at 4:50 AM
Reposted by Adrià Sogues
Our review on contact-dependent #predation is now out ! We define predation processes and describe how predators kill and eat their prey 😈 Repost appreciated ❤️
July 25, 2025 at 8:51 PM
Reposted by Adrià Sogues
Bacterial cell wall & periplasm paradigm shift - important read #MicroSky

www.nature.com/articles/s41...
July 29, 2025 at 4:01 PM
Reposted by Adrià Sogues
#MicroSky Summer read!

Predatory bacteria kill and eat other bacteria. Their deadly actions come in many flavors, often via direct physical contact between predator &
prey 🧛

How do they do it?
Our review 📖 @coralietesseur.bsky.social @ysantin.bsky.social

www.sciencedirect.com/science/arti...
Strategies and mechanisms of contact-dependent predation in bacteria
Predatory bacteria kill other bacteria to acquire nutrients. Among diverse strategies to kill and feed on prey, contact-dependent predation stands out…
www.sciencedirect.com
July 25, 2025 at 4:59 PM
Reposted by Adrià Sogues
New review from our lab! Compartmentalization during bacterial spore formation. Focusing on latest discoveries in Bacillus subtilis #subtiwiki

www.sciencedirect.com/science/arti...
July 9, 2025 at 5:28 AM
Reposted by Adrià Sogues
Folddisco finds similar (dis)continuous 3D motifs in large protein structure databases. Its efficient index enables fast uncharacterized active site annotation, protein conformational state analysis and PPI interface comparison. 1/9🧶🧬
📄 www.biorxiv.org/content/10.1...
🌐 search.foldseek.com/folddisco
July 7, 2025 at 8:21 AM
Reposted by Adrià Sogues
You thought all OMPs were trimeric? 🧐 Not in our predator B. bacteriovorus! 🦠
Excited to have contributed to this great collab with the Lovering lab on this intriguing protein. @lalouxlab.bsky.social
👉 Paper here!
www.nature.com/articles/s41...

#BacterialPredation #Bdellovibrio #StructuralBiology
A porin-like protein used by bacterial predators defines a wider lipid-trapping superfamily - Nature Communications
This study reveals that an outer membrane protein from the predator Bdellovibrio bacteriovorus forms a pentameric assembly that traps a lipid monolayer within. This allows the discovery of two superfa...
www.nature.com
July 7, 2025 at 6:58 AM
Reposted by Adrià Sogues
a treat for fans of 𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 𝘴𝘶𝘣𝘵𝘪𝘭𝘪𝘴 and its intriguing spores 👇
#MicroSky
July 4, 2025 at 7:49 PM
Reposted by Adrià Sogues
Running Phenix and stuck?
Now you can ask the new Phenix chatbot anything from the 600-page tutorial about how to run any program.
Big thanks to Tom Willinger for making this happen!
Try it out: phenix-online.org/version_docs...
June 23, 2025 at 5:15 AM
Reposted by Adrià Sogues
🎶 Science meets creativity with TuberKiller! Yaëlle Wormser, PhD student featured in the My PhD at the Institut Pasteur series, raps her tuberculosis research.

Discover how music and science collide to make research unforgettable! 👏

🎧 TuberKiller is also available on Spotify!

@pasteur.fr
When science meets creativity: TuberKiller | My PhD at the Institut Pasteur
YouTube video by Institut Pasteur EDUCATION
youtube.com
June 20, 2025 at 8:30 AM
Reposted by Adrià Sogues
Excited to share our latest review! Check it out to see what we know so far about how Staphylococcus aureus coordinates key processes during its cell cycle
June 9, 2025 at 11:57 AM
Reposted by Adrià Sogues
How does your favorite species elongate? 🧵 "Phenotypic plasticity in bacterial elongation among closely related species". Happy to see this paper in print @natcomms.nature.com. Nice work by @mariedelaby.bsky.social, Liu Yang et al. See original 🧵, different colours, same data and conclusions.
June 2, 2025 at 3:54 PM
Reposted by Adrià Sogues
The archaeal S-layer is not only pretty, it also carries crucial cellular functions, and the list keeps growing! @sshamphavi.bsky.social @marleenvw.bsky.social @archaellum.bsky.social and I wrote a little sheath sheet (+10 pts if you got the pun)
📖 Curr Opin Cell Biol
🔗 doi.org/10.1016/j.ce...
May 29, 2025 at 4:35 PM
I’m thrilled to finally share this preprint! It contains a WOW Cryo-EM structure (likely one of the strongest protein fibers known to man 🤯), but we’ve also untangled its biological function: a novel type of virulence factor💀!
www.researchsquare.com/article/rs-6...
May 20, 2025 at 9:37 AM