Félix Ramos-León
@felixrl.bsky.social
Postdoctoral fellow at NIH studying cell biology of coccoid bacteria.
Views my own.
He/him/Él 🏳️🌈
Views my own.
He/him/Él 🏳️🌈
Pinned
Félix Ramos-León
@felixrl.bsky.social
· Oct 28
PcdA promotes orthogonal division plane selection in Staphylococcus aureus
Nature Microbiology - PcdA interacts with DivIVA and FtsZ, promoting Z-ring formation and division plane selection in Staphylococcus aureus, which increases virulence in mice and reduces...
rdcu.be
Excited to share that our paper is now out in Nature Microbiology @natureportfolio.bsky.social! We characterize a positive mechanism for Z ring placement in S. aureus governed by the proteins PcdA/DivIVA, and its implication during infection in a mouse model #Microsky
rdcu.be/dYmdC
rdcu.be/dYmdC
Reposted by Félix Ramos-León
Reposted by Félix Ramos-León
The sporulation phosphorelay protein Spo0B is membrane localized in Paenibacillus! The transmembrane domain appears to increase interaction with relay partners. Congrats to former lab undergrad @isabellalin.bsky.social and her grad mentor @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
journals.asm.org
December 30, 2025 at 1:50 PM
The sporulation phosphorelay protein Spo0B is membrane localized in Paenibacillus! The transmembrane domain appears to increase interaction with relay partners. Congrats to former lab undergrad @isabellalin.bsky.social and her grad mentor @cassidyprints.bsky.social
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Reposted by Félix Ramos-León
A free, open-access library of high-quality organism illustrations for science communication
A free, open-access library of high-quality organism illustrations for science communication
We create vector graphics of model organisms and emerging biological research organisms to enhance our publications. We’re sharing these editable graphics under a CC0 license for other scientists to...
doi.org
December 29, 2025 at 6:03 PM
A free, open-access library of high-quality organism illustrations for science communication
Reposted by Félix Ramos-León
Reposted by Félix Ramos-León
New preprint. Work led by @ainsley-beaton.bsky.social & Rebecca Devine.
They show the highly conserved Streptomyces MtrAB two component system activates ectoine production and triggers sporulation in response to osmotic stress in Streptomyces venezuelae
www.biorxiv.org/content/10.6...
They show the highly conserved Streptomyces MtrAB two component system activates ectoine production and triggers sporulation in response to osmotic stress in Streptomyces venezuelae
www.biorxiv.org/content/10.6...
MtrAB activates ectoine production and triggers sporulation in response to osmotic stress in Streptomyces venezuelae
The MtrAB two-component system is a master regulator of antibiotic biosynthesis in Streptomyces species. MtrA is also required for sporulation under certain growth conditions, which means that on some...
www.biorxiv.org
December 22, 2025 at 7:19 AM
New preprint. Work led by @ainsley-beaton.bsky.social & Rebecca Devine.
They show the highly conserved Streptomyces MtrAB two component system activates ectoine production and triggers sporulation in response to osmotic stress in Streptomyces venezuelae
www.biorxiv.org/content/10.6...
They show the highly conserved Streptomyces MtrAB two component system activates ectoine production and triggers sporulation in response to osmotic stress in Streptomyces venezuelae
www.biorxiv.org/content/10.6...
Reposted by Félix Ramos-León
Colibactin produced by a honeybee symbiont defends against pathogens and shapes the gut community https://www.biorxiv.org/content/10.64898/2025.12.27.695564v1
December 28, 2025 at 1:16 AM
Colibactin produced by a honeybee symbiont defends against pathogens and shapes the gut community https://www.biorxiv.org/content/10.64898/2025.12.27.695564v1
Reposted by Félix Ramos-León
Coordination of chromosome segregation and cell division in the archaeon Sulfolobus acidocaldarius www.nature.com/articles/s41... #jcampubs
Coordination of chromosome segregation and cell division in the archaeon Sulfolobus acidocaldarius - Nature Communications
Very little is known about the molecular basis of chromosome segregation in archaea. Here, the authors describe conformational changes in the chromosome during the cell cycle of the archaeon Sulfolobu...
www.nature.com
December 24, 2025 at 9:29 PM
Coordination of chromosome segregation and cell division in the archaeon Sulfolobus acidocaldarius www.nature.com/articles/s41... #jcampubs
Reposted by Félix Ramos-León
Today, we held the workshop 'Young Bioscience Researchers: experiences from abroad' at the @cbm-csic-uam.bsky.social
An inspiring day featuring more than 20 scientific talks and networking opportunities, which allowed us to explore talent attraction opportunities.
An inspiring day featuring more than 20 scientific talks and networking opportunities, which allowed us to explore talent attraction opportunities.
December 22, 2025 at 4:28 PM
Today, we held the workshop 'Young Bioscience Researchers: experiences from abroad' at the @cbm-csic-uam.bsky.social
An inspiring day featuring more than 20 scientific talks and networking opportunities, which allowed us to explore talent attraction opportunities.
An inspiring day featuring more than 20 scientific talks and networking opportunities, which allowed us to explore talent attraction opportunities.
Reposted by Félix Ramos-León
MtrAB activates ectoine production and triggers sporulation in response to osmotic stress in Streptomyces venezuelae https://www.biorxiv.org/content/10.64898/2025.12.19.695424v1
December 22, 2025 at 6:16 AM
MtrAB activates ectoine production and triggers sporulation in response to osmotic stress in Streptomyces venezuelae https://www.biorxiv.org/content/10.64898/2025.12.19.695424v1
Reposted by Félix Ramos-León
A major challenge in RNA delivery is overcoming poor loading efficiency into nanoparticles. F. Machinandiarena solves this by using "SSHELs", our particles inspired by bacterial spores, to deliver RNA specifically to HER2+ ovarian cancer cells.
kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...
kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...
kwnsfk27.r.eu-west-1.awstrack.me
December 20, 2025 at 4:11 PM
A major challenge in RNA delivery is overcoming poor loading efficiency into nanoparticles. F. Machinandiarena solves this by using "SSHELs", our particles inspired by bacterial spores, to deliver RNA specifically to HER2+ ovarian cancer cells.
kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...
kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...
Reposted by Félix Ramos-León
What is better than one? Two connected papers!
#NewResearch
S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.
#MicroSky 🦠
www.nature.com/articles/s41...
#NewResearch
S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.
#MicroSky 🦠
www.nature.com/articles/s41...
December 19, 2025 at 2:06 PM
What is better than one? Two connected papers!
#NewResearch
S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.
#MicroSky 🦠
www.nature.com/articles/s41...
#NewResearch
S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division.
#MicroSky 🦠
www.nature.com/articles/s41...
Reposted by Félix Ramos-León
December 19, 2025 at 10:31 AM
Reposted by Félix Ramos-León
#NewResearch
Streptococcus equi subsp. zooepidemicus imports glucose via the bacterial mannose phosphotransferase system (PTSman), which inhibits stringent response, supports growth in cerebrospinal fluid and promotes brain damage during meningitis.
#MicroSky 🦠
www.nature.com/articles/s41...
Streptococcus equi subsp. zooepidemicus imports glucose via the bacterial mannose phosphotransferase system (PTSman), which inhibits stringent response, supports growth in cerebrospinal fluid and promotes brain damage during meningitis.
#MicroSky 🦠
www.nature.com/articles/s41...
Zoonotic Streptococcus imports glucose to inhibit stringent response and promote growth during meningitis - Nature Microbiology
Streptococcus equi subsp. zooepidemicus imports glucose via the bacterial mannose phosphotransferase system (PTSman), which inhibits stringent response, supports growth in cerebrospinal fluid and prom...
www.nature.com
December 18, 2025 at 3:06 PM
#NewResearch
Streptococcus equi subsp. zooepidemicus imports glucose via the bacterial mannose phosphotransferase system (PTSman), which inhibits stringent response, supports growth in cerebrospinal fluid and promotes brain damage during meningitis.
#MicroSky 🦠
www.nature.com/articles/s41...
Streptococcus equi subsp. zooepidemicus imports glucose via the bacterial mannose phosphotransferase system (PTSman), which inhibits stringent response, supports growth in cerebrospinal fluid and promotes brain damage during meningitis.
#MicroSky 🦠
www.nature.com/articles/s41...
Reposted by Félix Ramos-León
🧵Enterococcus faecalis is not just an extracellular organism. 🧵 A growing body of work shows it can survive + REPLICATE inside host cells, and that intracellular life may seed persistence, dissemination, and reinfection. 1/n #MMBR @asm.org journals.asm.org/doi/10.1128/...
Enterococcus faecalis: an overlooked cell invader | Microbiology and Molecular Biology Reviews
SUMMARYEnterococcus faecalis and Enterococcus faecium are human pathobionts that exhibit a dual lifestyle as commensal and pathogenic bacteria. The pathogenic lifestyle is associated with specific conditions involving host susceptibility and intestinal ...
journals.asm.org
December 18, 2025 at 9:19 AM
🧵Enterococcus faecalis is not just an extracellular organism. 🧵 A growing body of work shows it can survive + REPLICATE inside host cells, and that intracellular life may seed persistence, dissemination, and reinfection. 1/n #MMBR @asm.org journals.asm.org/doi/10.1128/...
Reposted by Félix Ramos-León
Too excited by the science to include the link to the paper! Here it is: journals.asm.org/doi/10.1128/...
December 16, 2025 at 4:30 AM
Too excited by the science to include the link to the paper! Here it is: journals.asm.org/doi/10.1128/...
Reposted by Félix Ramos-León
Fer’s tour de force in B. anthracis is out! Fer got Tn-seq running, built an ordered knockout library, defined all essential sporulation genes, and found a peptidoglycan deacetylase inhibitor critical for engulfment. Including our first one-by-all Alphafold screen! journals.plos.org/plosbiology/...
Identification of sporulation genes in Bacillus anthracis highlights similarities and significant differences with Bacillus subtilis
How good is Bacillus subtilis as a model for the spore-forming pathogen Bacillus anthracis? Using high throughput genetics to identify B. anthracis sporulation genes and cytological analysis of the mu...
journals.plos.org
December 13, 2025 at 4:53 PM
Fer’s tour de force in B. anthracis is out! Fer got Tn-seq running, built an ordered knockout library, defined all essential sporulation genes, and found a peptidoglycan deacetylase inhibitor critical for engulfment. Including our first one-by-all Alphafold screen! journals.plos.org/plosbiology/...
Reposted by Félix Ramos-León
Just got word that we are a go for the Bacterial Cell Biology and Development GRS and GRC at Southern New Hampshire University!
GRS will be on June 5-6, 2027
GRC will be from June 6-11, 2027
Block off your calendars Prokaryotic Cell Biologists!
More details to come! Please repost!
GRS will be on June 5-6, 2027
GRC will be from June 6-11, 2027
Block off your calendars Prokaryotic Cell Biologists!
More details to come! Please repost!
December 12, 2025 at 2:58 PM
Just got word that we are a go for the Bacterial Cell Biology and Development GRS and GRC at Southern New Hampshire University!
GRS will be on June 5-6, 2027
GRC will be from June 6-11, 2027
Block off your calendars Prokaryotic Cell Biologists!
More details to come! Please repost!
GRS will be on June 5-6, 2027
GRC will be from June 6-11, 2027
Block off your calendars Prokaryotic Cell Biologists!
More details to come! Please repost!
Reposted by Félix Ramos-León
indeed, 𝘙𝘶𝘨𝘰𝘴𝘪𝘵𝘢𝘭𝘦𝘢 𝘰𝘳𝘺𝘻𝘢𝘦 looke like fusilli, which are notably better at holding on salsa di pomodoro than spaghetti... and as pasta addicts, we fell instantly in love with the shape of this newly described alphaproteo from the Rhizobiales 🤭
#MicroSky
#MicroSky
December 10, 2025 at 7:52 PM
indeed, 𝘙𝘶𝘨𝘰𝘴𝘪𝘵𝘢𝘭𝘦𝘢 𝘰𝘳𝘺𝘻𝘢𝘦 looke like fusilli, which are notably better at holding on salsa di pomodoro than spaghetti... and as pasta addicts, we fell instantly in love with the shape of this newly described alphaproteo from the Rhizobiales 🤭
#MicroSky
#MicroSky
Reposted by Félix Ramos-León
It is rare as an editor to get to take on an article so uniquely in your wheelhouse. Sharing some wonderful work by Guarnaccia et al. from the Perez, Ng, and Neiditch labs. #structuralbiology of #pathogens @plos.org
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
Structure-function studies of Vibrio cholerae quorum-sensing receptor CqsR signal recognition
Author summary Quorum sensing, a form of bacterial cell-cell communication, regulates diverse developmental behaviors. This study investigates CqsR, a transmembrane receptor in Vibrio cholerae, the ga...
dx.plos.org
September 8, 2025 at 5:13 PM
It is rare as an editor to get to take on an article so uniquely in your wheelhouse. Sharing some wonderful work by Guarnaccia et al. from the Perez, Ng, and Neiditch labs. #structuralbiology of #pathogens @plos.org
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
Reposted by Félix Ramos-León
Spx inhibits expression of the SwrA•DegU master flagellar activator in Bacillus subtilis
-in JBacteriol
journals.asm.org/doi/10.1128/...
-in JBacteriol
journals.asm.org/doi/10.1128/...
Spx inhibits expression of the SwrA•DegU master flagellar activator in Bacillus subtilis | Journal of Bacteriology
Bacterial flagella are elaborate machines that are inserted through the bacterial
cell envelope. The regulation of flagellar gene expression is organized in hierarchical
tiers that largely correlate w...
journals.asm.org
December 6, 2025 at 8:36 PM
Spx inhibits expression of the SwrA•DegU master flagellar activator in Bacillus subtilis
-in JBacteriol
journals.asm.org/doi/10.1128/...
-in JBacteriol
journals.asm.org/doi/10.1128/...
Reposted by Félix Ramos-León
Ten PhD contracts at CSIC in Synthetic Biology.
Passionate about biophysics or computational molecular biology? Join Daniel Lopez's @mib-lab.bsky.social and me at @cnb-csic.bsky.social studying bacterial membrane microdomains!
lifehub.csic.es/synbio-cofund/
(Please spread the word, thanks!)
Passionate about biophysics or computational molecular biology? Join Daniel Lopez's @mib-lab.bsky.social and me at @cnb-csic.bsky.social studying bacterial membrane microdomains!
lifehub.csic.es/synbio-cofund/
(Please spread the word, thanks!)
General Synbio Cofund | Life HUB CSIC
SYNBIO-CSIC (CSIC Doctoral Programme in Synthetic Biology: Engineering Biomolecular and Cellular Systems) is an innovative, leading-edge doctoral programme led by the Spanish National Research Council (CSIC) and co-funded by the European Union.
lifehub.csic.es
December 4, 2025 at 2:34 PM
Ten PhD contracts at CSIC in Synthetic Biology.
Passionate about biophysics or computational molecular biology? Join Daniel Lopez's @mib-lab.bsky.social and me at @cnb-csic.bsky.social studying bacterial membrane microdomains!
lifehub.csic.es/synbio-cofund/
(Please spread the word, thanks!)
Passionate about biophysics or computational molecular biology? Join Daniel Lopez's @mib-lab.bsky.social and me at @cnb-csic.bsky.social studying bacterial membrane microdomains!
lifehub.csic.es/synbio-cofund/
(Please spread the word, thanks!)
Reposted by Félix Ramos-León
The diderm cell envelope is not a stack of layers but a unified scaffold of Inner Membrane–Peptidoglycan–Outer Membrane.
We discuss how tethering the OM to the PG in E. coli preserves integrity — and extend the concept across diderm bacteria.
Curr Opin Microbiol: doi.org/10.1016/j.mi...
#microsky 🔬
We discuss how tethering the OM to the PG in E. coli preserves integrity — and extend the concept across diderm bacteria.
Curr Opin Microbiol: doi.org/10.1016/j.mi...
#microsky 🔬
Redirecting
doi.org
November 13, 2025 at 12:17 PM
The diderm cell envelope is not a stack of layers but a unified scaffold of Inner Membrane–Peptidoglycan–Outer Membrane.
We discuss how tethering the OM to the PG in E. coli preserves integrity — and extend the concept across diderm bacteria.
Curr Opin Microbiol: doi.org/10.1016/j.mi...
#microsky 🔬
We discuss how tethering the OM to the PG in E. coli preserves integrity — and extend the concept across diderm bacteria.
Curr Opin Microbiol: doi.org/10.1016/j.mi...
#microsky 🔬
Reposted by Félix Ramos-León
A 3UTR-derived small RNA modulates the life-cycle of the cholera toxin-encoding filamentous phage, CTXphi https://www.biorxiv.org/content/10.64898/2025.12.02.691856v1
December 3, 2025 at 3:16 AM
A 3UTR-derived small RNA modulates the life-cycle of the cholera toxin-encoding filamentous phage, CTXphi https://www.biorxiv.org/content/10.64898/2025.12.02.691856v1
Reposted by Félix Ramos-León
Cool work from Heath Murray's lab where they use Cas9 nickase to study how ssDNA breaks are repaired in Bacillus subtilis #MicroSky www.nature.com/articles/s41...
Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading - Nature Communications
DNA damage can lead to cell death. Here, the authors show that a simple cut on either strand of DNA can inactivate bacterial chromosome replication. Surprisingly, only a core set of recombination prot...
www.nature.com
November 28, 2025 at 2:25 PM
Cool work from Heath Murray's lab where they use Cas9 nickase to study how ssDNA breaks are repaired in Bacillus subtilis #MicroSky www.nature.com/articles/s41...
Reposted by Félix Ramos-León
Happy to share a new preprint from the lab: Marko became interested in whether the disordered linker of FtsZ might influence how the protein organizes into the Z-ring. And this led to some surprising findings!
Preprint: www.biorxiv.org/content/10.1...
Preprint: www.biorxiv.org/content/10.1...
Charge segregation in the C-terminal linker of FtsZ enables Z-ring scaling with prokaryotic cell width
Bacterial cytokinesis is orchestrated by the Z-ring, a cytoskeletal structure formed by treadmilling filaments of the tubulin-like GTPase FtsZ. During assembly, filament curvature must match the cell ...
www.biorxiv.org
November 26, 2025 at 9:29 PM
Happy to share a new preprint from the lab: Marko became interested in whether the disordered linker of FtsZ might influence how the protein organizes into the Z-ring. And this led to some surprising findings!
Preprint: www.biorxiv.org/content/10.1...
Preprint: www.biorxiv.org/content/10.1...