Emmanuele Severi
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emmseveri.bsky.social
Emmanuele Severi
@emmseveri.bsky.social
Research Fellow at Northumbria University: phage, synthetic biology, sialic acid, transport proteins. He/him.
Reposted by Emmanuele Severi
So happy to share this! Bacteriocins were first discovered over 100 years ago, but what do they actually do? We look at >1000 bacteriocin plasmids and find links to virulence and antimicrobial resistance, and frequent bacteriocin sharing in Enterobacteriaceae.
www.nature.com/articles/s41...
Bacterial warfare is associated with virulence and antimicrobial resistance - Nature Communications
Bacteria employ a range of competition systems that deliver toxins to inhibit competing strains. This study shows that these systems are particularly important for the ecology of virulent and antibiot...
www.nature.com
November 9, 2025 at 4:51 PM
Reposted by Emmanuele Severi
Occurrence of type VI secretion system effector genes in longitudinal isolates of P. aeruginosa from people with cystic fibrosis

Microbial Genomics by Antonia Habich from Daniel Unterweger
with @asgriffin.bsky.social @sandrabandersen.bsky.social

www.microbiologyresearch.org/content/jour...
Occurrence of type VI secretion system effector genes in longitudinal isolates of P. aeruginosa from people with cystic fibrosis
Pseudomonas aeruginosa uses multiple type VI secretion systems (T6SSs) to manipulate eukaryotic cells, kill competing microbes and take up nutrients. Bacterial strains are known to differ in their T6S...
www.microbiologyresearch.org
November 8, 2025 at 11:43 AM
Reposted by Emmanuele Severi
Endolysin of Aeromonas bacteriophage 3: a novel Ca(2+)-activated peptidoglycan hydrolase giving insight into the regulation of phage lysis in Gram-negative bacteria.

DOI: 10.1016/j.ijbiomac.2025.146934

https://pubmed.ncbi.nlm.nih.gov/40825433/
Endolysin of Aeromonas bacteriophage 3: a novel Ca2+-activated peptidoglycan hydrolase giving insight into the regulation of phage lysis in Gram-negative bacteria - PubMed
A search for bacteriophage proteins with an EF-like calcium-binding loop, first discovered in the phage T5 endolysin (EndoT5), revealed a group of 71 peptidases of the M15_C subfamily. All the sequences found belonged to the phages infecting Gram-negative bacteria (Escherichia, Salmonella, Klebsiell …
pubmed.ncbi.nlm.nih.gov
November 7, 2025 at 7:30 PM
As @giusym1990.bsky.social puts it, bacteria know when there’s a deadline!!!
The most productive week: given a strain that’s intrinsically resistant to the antibiotic I must use; given a different one that doesn’t grow at all; synthetic gene has a mutation; had to test 3 different polymerases for the PCR to work.

Is it wine o’clock yet?!?
November 7, 2025 at 4:56 PM
The most productive week: given a strain that’s intrinsically resistant to the antibiotic I must use; given a different one that doesn’t grow at all; synthetic gene has a mutation; had to test 3 different polymerases for the PCR to work.

Is it wine o’clock yet?!?
November 7, 2025 at 4:55 PM
www.biorxiv.org
November 7, 2025 at 12:33 PM
#synbio significant technological development of large Ser recombinases.

I’m sure @factor50.bsky.social would love to have a look

www.nature.com/articles/s41...
Site-specific DNA insertion into the human genome with engineered recombinases - Nature Biotechnology
Engineered DNA recombinases efficiently and specifically insert genetic cargos without the use of landing pads.
www.nature.com
November 7, 2025 at 8:16 AM
Reposted by Emmanuele Severi
Looks awesome! Junior scientists -- apply!
The full list of workshop instructors is available here.

Registration closes November 15 🥳🥳
bsky.app/profile/jlst...
November 7, 2025 at 2:52 AM
Reposted by Emmanuele Severi
🚨New preprint out!
We present a foundational genomic resource of human gut microbiome viruses. It delivers high-quality, deeply curated data spanning taxonomy, predicted hosts, structures, and functions, providing a reference for gut virome research. (1/8)
www.biorxiv.org/content/10.1...
November 6, 2025 at 5:26 PM
Reposted by Emmanuele Severi
Amazing paper, especially love this figure of what differentiates broad host range phages: especially Diversity Generating Retroelements and multiple methyltransferases
November 7, 2025 at 3:01 AM
Reposted by Emmanuele Severi
New paper: www.sciencedirect.com/science/arti...
Klebs can evolve resistance to Colistin through mgrB mutation or by acquiring the mcr-1 gene. Both routes have similar fitness costs against a bacterial competitor, but mgrB mutations are associated with enhanced virulence.
mgrB inactivation confers enhanced pathogenicity and immune evasion over mcr-1 expression in colistin-resistant Klebsiella pneumoniae
Colistin is one of the last treatment options against human infections caused by multi-drug resistant Klebsiella pneumoniae. Colistin resistant K. pne…
www.sciencedirect.com
November 6, 2025 at 12:13 PM
Reposted by Emmanuele Severi
#ISMEJournal paper from Simon Heilbronner

Competitive fitness of Staphylococcus aureus against nasal commensals depends on biotin biosynthesis and acquisition

academic.oup.com/ismej/advanc...
Competitive fitness of Staphylococcus aureus against nasal commensals depends on biotin biosynthesis and acquisition
Abstract. The human nasal microbiome can serve as a reservoir for pathogens. In particular, the opportunistic pathogen Staphylococcus aureus can be a membe
academic.oup.com
November 6, 2025 at 8:38 AM