T3SS
@t3sss.bsky.social
Manually curated posts on type III secretion system. A project managed by @e_sotog now at @ccg_unam
https://www.ccg.unam.mx/en/jose-eduardo-soto-guzman/
https://www.ccg.unam.mx/en/jose-eduardo-soto-guzman/
Reposted by T3SS
📣New preprint with @rberntsson.bsky.social👇We overturn the long-standing view that Gram-positive bacteria lack conjugative pili! We identify pili-forming proteins in a wide range of bacteria and show they're essential for the spread of #antibioticresistance #AMR 🧵1/6
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Pili are essential for conjugation also in many Gram-positive bacteria
Type IV secretion systems (T4SS) enable the spread of antibiotic resistance and other virulence factors. In Gram-positive bacteria, T4SSs have long been thought to lack VirB2-like proteins that form c...
www.biorxiv.org
December 30, 2025 at 6:03 PM
📣New preprint with @rberntsson.bsky.social👇We overturn the long-standing view that Gram-positive bacteria lack conjugative pili! We identify pili-forming proteins in a wide range of bacteria and show they're essential for the spread of #antibioticresistance #AMR 🧵1/6
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Assembly and glycosylation of Helicobacter pylori sheathed flagella
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Assembly and glycosylation of Helicobacter pylori sheathed flagella
The bacterial flagellum is a complex nanomachine essential for motility, colonization, and invasion in diverse species. Helicobacter pylori has evolved elaborate sheathed flagella that enable migratio...
www.biorxiv.org
December 31, 2025 at 6:36 PM
Assembly and glycosylation of Helicobacter pylori sheathed flagella
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Cyclic-di-GMP controls Type III effector export and symptom development in Pseudomonas syringae infections via the export ATPase HrcN
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
Cyclic-di-GMP controls Type III effector export and symptom development in Pseudomonas syringae infections via the export ATPase HrcN
Author summary Pseudomonas syringae is a bacterial plant pathogen that uses a molecular needle; the type 3 injectisome (T3I), to secrete specialised effector proteins into plant cells. Effector secret...
dx.plos.org
December 30, 2025 at 4:34 PM
Cyclic-di-GMP controls Type III effector export and symptom development in Pseudomonas syringae infections via the export ATPase HrcN
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
Enteropathogenic E. coli-mediated Fast and Coordinated Ca²⁺ responses regulate NF-κB activation
doi.org/10.7554/eLif...
doi.org/10.7554/eLif...
doi.org
December 29, 2025 at 6:15 PM
Enteropathogenic E. coli-mediated Fast and Coordinated Ca²⁺ responses regulate NF-κB activation
doi.org/10.7554/eLif...
doi.org/10.7554/eLif...
Ultraweak interactions drive cap-mediated positioning and elongation of the bacterial flagellar filament
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Ultraweak interactions drive cap-mediated positioning and elongation of the bacterial flagellar filament - Nature Communications
By integrating NMR and cryo-EM, this study resolves how the bacterial flagellar filament assembles despite a symmetry mismatch, revealing that ultra-weak interactions and a rotary cap mediated mechani...
www.nature.com
December 26, 2025 at 7:21 PM
Ultraweak interactions drive cap-mediated positioning and elongation of the bacterial flagellar filament
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Dynamic interaction of the Yersinia pseudotuberculosis type three secretion system proteins LcrV and LcrG - Mangu - 2026 - Protein Science - Wiley Online Library onlinelibrary.wiley.com/doi/full/10....
onlinelibrary.wiley.com
December 23, 2025 at 1:56 AM
Dynamic interaction of the Yersinia pseudotuberculosis type three secretion system proteins LcrV and LcrG - Mangu - 2026 - Protein Science - Wiley Online Library onlinelibrary.wiley.com/doi/full/10....
TTSS-2 virulence drives inflammatory destruction of the gut epithelial barrier and modulates inflammatory response profiles in the Salmonella-infected mouse guthttps://dx.plos.org/10.1371/journal.ppat.1013733
TTSS-2 virulence drives inflammatory destruction of the gut epithelial barrier and modulates inflammatory response profiles in the Salmonella-infected mouse gut
Author summary The intestinal lining forms a crucial barrier that protects the body from potentially harmful microbes in the gut. Enteric pathogens like Salmonella enterica serovar Typhimurium (S. Tm)...
dx.plos.org
December 14, 2025 at 11:09 PM
TTSS-2 virulence drives inflammatory destruction of the gut epithelial barrier and modulates inflammatory response profiles in the Salmonella-infected mouse guthttps://dx.plos.org/10.1371/journal.ppat.1013733
Checkout this article I found at PLOS: dx.plos.org/10.1371/jour...
TTSS-2 virulence drives inflammatory destruction of the gut epithelial barrier and modulates inflammatory response profiles in the Salmonella-infected mouse gut
Author summary The intestinal lining forms a crucial barrier that protects the body from potentially harmful microbes in the gut. Enteric pathogens like Salmonella enterica serovar Typhimurium (S. Tm)...
dx.plos.org
December 11, 2025 at 2:57 PM
Checkout this article I found at PLOS: dx.plos.org/10.1371/jour...
Unravelling Salmonella Pathogenesis: Effector-Mediated Post-Translational Modifications, Alternative Therapies, and Advances in Rapid Detection | ACS Infectious Diseases pubs.acs.org/doi/10.1021/...
Unravelling Salmonella Pathogenesis: Effector-Mediated Post-Translational Modifications, Alternative Therapies, and Advances in Rapid Detection
Salmonella infections remain a priority concern in both developed and developing regions due to antimicrobial resistance and the lack of proper diagnosis. While substantial progress has been made in d...
pubs.acs.org
December 10, 2025 at 2:48 PM
Unravelling Salmonella Pathogenesis: Effector-Mediated Post-Translational Modifications, Alternative Therapies, and Advances in Rapid Detection | ACS Infectious Diseases pubs.acs.org/doi/10.1021/...
Exploiting Pseudomonas syringae Type 3 Secretion to Study Effector Contribution to Disease in Spinach | Molecular Plant-Microbe Interactions® apsjournals.apsnet.org/doi/10.1094/...
Exploiting Pseudomonas syringae Type 3 Secretion to Study Effector Contribution to Disease in Spinach | Molecular Plant-Microbe Interactions®
Intensive spinach cultivation creates favorable conditions for the emergence and rapid evolution of pathogens, leading to substantial economic losses. Research on host–pathogen interactions in leafy g...
apsjournals.apsnet.org
December 10, 2025 at 2:48 PM
Exploiting Pseudomonas syringae Type 3 Secretion to Study Effector Contribution to Disease in Spinach | Molecular Plant-Microbe Interactions® apsjournals.apsnet.org/doi/10.1094/...
Full article: The ubiquitin system targets translocated EspH to proteasomal degradation www.tandfonline.com/doi/full/10....
The ubiquitin system targets translocated EspH to proteasomal degradation
EspH is an effector protein secreted by the type III secretion system of various pathogenic Escherichia coli strains, including enteropathogenic E. coli (EPEC). The ability of EspH to inhibit host ...
www.tandfonline.com
December 10, 2025 at 4:25 AM
Full article: The ubiquitin system targets translocated EspH to proteasomal degradation www.tandfonline.com/doi/full/10....
Reposted by T3SS
An unexpected mechanism for population heterogeneity in the gut microbiome:
www.cell.com/cell-host-mi...
www.cell.com/cell-host-mi...
A commensal bacterium secretes effector proteins to establish population heterogeneity in the gut
Zagieboylo and colleagues demonstrate that the human commensal Bacteroides thetaiotaomicron
secretes two self-targeting proteins that induce heterogeneity within its own population.
Differential expre...
www.cell.com
December 3, 2025 at 3:39 AM
An unexpected mechanism for population heterogeneity in the gut microbiome:
www.cell.com/cell-host-mi...
www.cell.com/cell-host-mi...
Interplay between T3SS effectors, ExoY activation, and cGMP signaling in Pseudomonas aeruginosa infection
nature.com/articles/s41...
nature.com/articles/s41...
Interplay between T3SS effectors, ExoY activation, and cGMP signaling in Pseudomonas aeruginosa infection - Nature Communications
The Type 3 Secretion System (T3SS) of Pseudomonas aeruginosa injects effectors into host cells to promote infection. Here, the authors reveal a new regulatory role for the T3SS effector ExoY in modula...
nature.com
December 2, 2025 at 10:20 PM
Interplay between T3SS effectors, ExoY activation, and cGMP signaling in Pseudomonas aeruginosa infection
nature.com/articles/s41...
nature.com/articles/s41...
Bimodal expression of Type 3 Secretion System 2 enables cooperative virulence among intracellular Salmonella Typhimurium
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
dx.plos.org
December 2, 2025 at 2:50 PM
Bimodal expression of Type 3 Secretion System 2 enables cooperative virulence among intracellular Salmonella Typhimurium
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
Typhoid toxin of Salmonella Typhi elicits host antimicrobial response during acute typhoid fever | EMBO Molecular Medicine www.embopress.org/doi/full/10....
Typhoid toxin of Salmonella Typhi elicits host antimicrobial response during acute typhoid fever | EMBO Molecular Medicine
imageimageDNA damage responses (DDRs) counteract cancer but whether they defend against bacterial
infections is unclear. We provide evidence that DDRs by typhoid toxin of Salmonella Typhi elicits an a...
www.embopress.org
December 1, 2025 at 9:04 PM
Typhoid toxin of Salmonella Typhi elicits host antimicrobial response during acute typhoid fever | EMBO Molecular Medicine www.embopress.org/doi/full/10....
Reposted by T3SS
Role of FlhF and its domains in the assembly of a polar flagellum in P. aeruginosa
#JBacteriol from Deepti Jain
journals.asm.org/doi/10.1128/...
#JBacteriol from Deepti Jain
journals.asm.org/doi/10.1128/...
Role of FlhF and its domains in the assembly of a polar flagellum in P. aeruginosa | Journal of Bacteriology
Pathogenic bacteria rely on flagellar motility for infection and colonization. FlhF,
a member of the SRP GTPase family, is a key factor regulating flagellation patterns
in various bacteria. In this st...
journals.asm.org
November 27, 2025 at 12:23 PM
Role of FlhF and its domains in the assembly of a polar flagellum in P. aeruginosa
#JBacteriol from Deepti Jain
journals.asm.org/doi/10.1128/...
#JBacteriol from Deepti Jain
journals.asm.org/doi/10.1128/...
Actin isoform–specific interactions revealed by Vibrio VopV actin-binding repeats | PNAS www.pnas.org/doi/abs/10.1...
Actin isoform–specific interactions revealed by Vibrio VopV actin-binding repeats | PNAS
Despite an evolutionary separation of over 300 Mya, there are no amino acid substitutions
in certain actin isoforms from reptiles to mammals. What ...
www.pnas.org
November 26, 2025 at 2:16 AM
Actin isoform–specific interactions revealed by Vibrio VopV actin-binding repeats | PNAS www.pnas.org/doi/abs/10.1...
Reposted by T3SS
Secreting bacteria undergo drastic changes.
We found that in Yersinia, T3SS activation triggers rapid, large-scale reorganisation of chromosomal and plasmid DNA. This links secretion to growth inhibition-revealing a new connection between virulence and bacterial cell biology.
doi.org/10.1016/j.ce...
We found that in Yersinia, T3SS activation triggers rapid, large-scale reorganisation of chromosomal and plasmid DNA. This links secretion to growth inhibition-revealing a new connection between virulence and bacterial cell biology.
doi.org/10.1016/j.ce...
November 24, 2025 at 10:56 PM
Secreting bacteria undergo drastic changes.
We found that in Yersinia, T3SS activation triggers rapid, large-scale reorganisation of chromosomal and plasmid DNA. This links secretion to growth inhibition-revealing a new connection between virulence and bacterial cell biology.
doi.org/10.1016/j.ce...
We found that in Yersinia, T3SS activation triggers rapid, large-scale reorganisation of chromosomal and plasmid DNA. This links secretion to growth inhibition-revealing a new connection between virulence and bacterial cell biology.
doi.org/10.1016/j.ce...
Lithocholic acid induces T3SS-dependent formation of invasion-competent Shigella flexneri aggregates
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Lithocholic acid induces T3SS-dependent formation of invasion-competent Shigella flexneri aggregates
Shigella flexneri causes shigellosis, the second leading cause of diarrheal deaths worldwide. The pathogen invades colonic epithelial cells using a type III secretion system (T3SS) that delivers effec...
www.biorxiv.org
November 25, 2025 at 4:58 PM
Lithocholic acid induces T3SS-dependent formation of invasion-competent Shigella flexneri aggregates
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by T3SS
#MicrobiologyMonday: The bacterial flagellum is built through a hierarchical assembly process and powered by a dynamic motor capable of astonishing speeds. What's new in our understanding of these complex nanomachines? Find out in #MMBRJournal: asm.social/2HB
November 24, 2025 at 4:31 PM
#MicrobiologyMonday: The bacterial flagellum is built through a hierarchical assembly process and powered by a dynamic motor capable of astonishing speeds. What's new in our understanding of these complex nanomachines? Find out in #MMBRJournal: asm.social/2HB
Building the bacterial flagellum: coordinating regulation, dynamic assembly, and function | Microbiology and Molecular Biology Reviews journals.asm.org/doi/10.1128/...
Building the bacterial flagellum: coordinating regulation, dynamic assembly, and function | Microbiology and Molecular Biology Reviews
SUMMARYBacterial flagella are remarkable rotary machines that enable motility, environmental
sensing, and host interaction. In this review, we discuss recent advances in understanding
the structure, a...
journals.asm.org
November 23, 2025 at 6:19 PM
Building the bacterial flagellum: coordinating regulation, dynamic assembly, and function | Microbiology and Molecular Biology Reviews journals.asm.org/doi/10.1128/...
Flagellar location determines the stability of bacterial surface entrapment | PNAS www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
November 23, 2025 at 6:19 PM
Flagellar location determines the stability of bacterial surface entrapment | PNAS www.pnas.org/doi/10.1073/...
Swashing: a propulsion-independent form of bacterial surface migration | Journal of Bacteriology journals.asm.org/doi/10.1128/...
Swashing: a propulsion-independent form of bacterial surface migration | Journal of Bacteriology
Bacteria move on surfaces using a variety of mechanisms, with important implications
for their growth and survival in both the clinical setting (such as on the surface
of medical devices) and the wild...
journals.asm.org
November 21, 2025 at 7:01 PM
Swashing: a propulsion-independent form of bacterial surface migration | Journal of Bacteriology journals.asm.org/doi/10.1128/...
Reposted by T3SS
What if even the core of bacterial nanomachines wasn’t static?
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
🔗 www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
🔗 www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
November 12, 2025 at 4:21 PM
What if even the core of bacterial nanomachines wasn’t static?
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
🔗 www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
🔗 www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system - Nature Communications
Bacteria use the type III secretion system (T3SS) to inject proteins into target cells. Here, Brianceau et al. show that a core structural component, SctD, exchanges between a T3SS-bound and a freely ...
www.nature.com
November 11, 2025 at 9:28 PM
Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system
www.nature.com/articles/s41...
www.nature.com/articles/s41...