T3SS
@t3sss.bsky.social
Manually curated posts on type III secretion system. A project managed by @e_sotog now at @ccg_unam
https://www.ccg.unam.mx/en/jose-eduardo-soto-guzman/
https://www.ccg.unam.mx/en/jose-eduardo-soto-guzman/
Reposted by T3SS
What if even the core of bacterial nanomachines wasn’t static?
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
🔗 www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
🔗 www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
November 12, 2025 at 4:21 PM
What if even the core of bacterial nanomachines wasn’t static?
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
🔗 www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
🔗 www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system - Nature Communications
Bacteria use the type III secretion system (T3SS) to inject proteins into target cells. Here, Brianceau et al. show that a core structural component, SctD, exchanges between a T3SS-bound and a freely ...
www.nature.com
November 11, 2025 at 9:28 PM
Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structures of the sheathed flagellum reveal mechanisms of assembly and rotation in Vibrio cholerae
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structures of the sheathed flagellum reveal mechanisms of assembly and rotation in Vibrio cholerae - Nature Microbiology
In situ cryo-electron microscopy structures show the spatial arrangement of flagellin proteins and provide insight into mechanisms of assembly and rotation of the sheathed flagellum in Vibrio cholerae...
www.nature.com
November 7, 2025 at 5:49 PM
Structures of the sheathed flagellum reveal mechanisms of assembly and rotation in Vibrio cholerae
www.nature.com/articles/s41...
www.nature.com/articles/s41...
In situ structures of the Legionella Dot/Icm T4SS identify the DotA–IcmX complex as the gatekeeper for effector translocation | PNAS www.pnas.org/doi/10.1073/...
In situ structures of the Legionella Dot/Icm T4SS identify the DotA–IcmX complex as the gatekeeper for effector translocation | PNAS
The Dot/Icm machine of Legionella pneumophila is among the most versatile type IV
secretion systems (T4SSs), capable of translocating more than 330...
www.pnas.org
November 7, 2025 at 5:48 PM
In situ structures of the Legionella Dot/Icm T4SS identify the DotA–IcmX complex as the gatekeeper for effector translocation | PNAS www.pnas.org/doi/10.1073/...
Reposted by T3SS
Great collaboration with @guo-lab.bsky.social to determine an in situ architectural model of the Tad pilus machine. Be on the lookout soon for a preprint on the same topic by Grant Jensen and @viollierpat.bsky.social. #microsky
November 6, 2025 at 3:51 PM
Great collaboration with @guo-lab.bsky.social to determine an in situ architectural model of the Tad pilus machine. Be on the lookout soon for a preprint on the same topic by Grant Jensen and @viollierpat.bsky.social. #microsky
TXSelect: A multi-task learning model to identify secretory effectors
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
TXSelect: A multi-task learning model to identify secretory effectors
Author summary Secretory effectors are specialized proteins produced by pathogenic bacteria that allow them to infect host organisms by disrupting normal cellular functions. Accurately identifying and...
dx.plos.org
November 7, 2025 at 3:57 PM
TXSelect: A multi-task learning model to identify secretory effectors
dx.plos.org/10.1371/jour...
dx.plos.org/10.1371/jour...
Intracellular Salmonella hijacks the mitochondrial citrate carrier to evade host oxidative defenses
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Intracellular Salmonella hijacks the mitochondrial citrate carrier to evade host oxidative defenses - Nature Communications
In this study, the authors show that Salmonella enterica serovar Typhimurium co-opts the host mitochondrial citrate carrier (CIC) to regulate the redox state of its vacuole, facilitating intracellular...
www.nature.com
November 6, 2025 at 11:54 PM
Intracellular Salmonella hijacks the mitochondrial citrate carrier to evade host oxidative defenses
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Salmonella multimutants enable efficient identification of SPI-2 effector protein function in gut inflammation and systemic colonization
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Salmonella multimutants enable efficient identification of SPI-2 effector protein function in gut inflammation and systemic colonization - Nature Communications
Intracellular Salmonella enterica uses a type III secretion system to secrete more than 30 effector proteins into host cells. Here, Newson et al. generate six mutant strains deficient in different set...
www.nature.com
November 6, 2025 at 11:54 PM
Salmonella multimutants enable efficient identification of SPI-2 effector protein function in gut inflammation and systemic colonization
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The Salmonella pathogenicity island 1 injectisome reprograms host cell translation to evade the inflammatory response
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The Salmonella pathogenicity island 1 injectisome reprograms host cell translation to evade the inflammatory response - Nature Communications
In this work, authors show that Salmonella uses its SPI-1 injectisome to infect macrophages, triggering rapid translational upregulation of transcription factors, such as EGR1 which suppresses the inf...
www.nature.com
November 5, 2025 at 3:57 PM
The Salmonella pathogenicity island 1 injectisome reprograms host cell translation to evade the inflammatory response
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Full article: Deletion of the hslV gene alleviates the virulence and lethality of Salmonella Typhimurium in hosts by modulating lon and T3SS-1 www.tandfonline.com/doi/full/10....
Deletion of the hslV gene alleviates the virulence and lethality of Salmonella Typhimurium in hosts by modulating lon and T3SS-1
Salmonella Typhimurium (S. Typhimurium) is a major foodborne pathogen that utilizes flagella-mediated biofilm formation and type III secretion systems (T3SSs) to initiate infections, contributing s...
www.tandfonline.com
November 4, 2025 at 3:40 PM
Full article: Deletion of the hslV gene alleviates the virulence and lethality of Salmonella Typhimurium in hosts by modulating lon and T3SS-1 www.tandfonline.com/doi/full/10....
Transcriptomic and proteomic analysis of the effect of hydrogen peroxide on enterohemorrhagic Escherichia coli: Heliyon www.cell.com/heliyon/full...
Transcriptomic and proteomic analysis of the effect of hydrogen peroxide on enterohemorrhagic Escherichia coli
Enterohemorrhagic Escherichia coli (EHEC) is an emerging pathogen responsible for
causing severe gastrointestinal infections in humans through foodborne transmission.
Previous research has demonstrate...
www.cell.com
November 1, 2025 at 9:42 PM
Transcriptomic and proteomic analysis of the effect of hydrogen peroxide on enterohemorrhagic Escherichia coli: Heliyon www.cell.com/heliyon/full...
Cooperative Binding of Cytosolic Type III Secretion System Proteins to the Injectisome Revealed by Live-Cell Single-Molecule Localization Microscopy
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
October 31, 2025 at 4:28 AM
Cooperative Binding of Cytosolic Type III Secretion System Proteins to the Injectisome Revealed by Live-Cell Single-Molecule Localization Microscopy
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Long-term control of Salmonella after transient T3SS-2 inhibition
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Long-term control of Salmonella after transient T3SS-2 inhibition
Anti-virulence approaches are promising alternatives for traditional antibiotics to control bacterial infections. Several inhibitors show impressive activities in animal infection models, but the rela...
www.biorxiv.org
October 31, 2025 at 4:27 AM
Long-term control of Salmonella after transient T3SS-2 inhibition
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Bacterial effector OspB hijacks apoptosis through peptide-bond recombination of BH3 domain proteins: Cell Host & Microbe www.cell.com/cell-host-mi...
Bacterial effector OspB hijacks apoptosis through peptide-bond recombination of BH3 domain proteins
Shao et al. show that the Shigella effector OspB blocks apoptosis by enzymatically
remodeling host BCL-2 proteins through peptide-bond recombination. This reveals a
unique pathogen strategy to rewire ...
www.cell.com
October 24, 2025 at 12:35 AM
Bacterial effector OspB hijacks apoptosis through peptide-bond recombination of BH3 domain proteins: Cell Host & Microbe www.cell.com/cell-host-mi...
IscR-mediated sensing of iron-sulfur cluster demand coordinates virulence gene expression in Yersinia pseudotuberculosis
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
IscR-mediated sensing of iron-sulfur cluster demand coordinates virulence gene expression in Yersinia pseudotuberculosis
The type III secretion system (T3SS) is a needle-like appendage that translocates effector proteins into host cells to disrupt host defenses. Strict control of T3SS expression is critical for facultat...
www.biorxiv.org
October 20, 2025 at 5:42 PM
IscR-mediated sensing of iron-sulfur cluster demand coordinates virulence gene expression in Yersinia pseudotuberculosis
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by T3SS
Celebrated every 15 October, Global Hand Washing Day aims to raise awareness of the importance of hand hygiene in curbing the transmission of certain diseases and the spread of antimicrobial resistance.
October 15, 2025 at 3:06 PM
Celebrated every 15 October, Global Hand Washing Day aims to raise awareness of the importance of hand hygiene in curbing the transmission of certain diseases and the spread of antimicrobial resistance.
Reposted by T3SS
New in JB: Déraspe, Roy et al. perform a comparative genomics study of the PA7-clade of P. aeruginosa - now recognized as a distinct species, Pseudomonas paraeruginosa. This analysis revealed 2 sub-clades, the lack of T3SS & multiresistance.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
October 3, 2025 at 2:04 PM
New in JB: Déraspe, Roy et al. perform a comparative genomics study of the PA7-clade of P. aeruginosa - now recognized as a distinct species, Pseudomonas paraeruginosa. This analysis revealed 2 sub-clades, the lack of T3SS & multiresistance.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology
T3SS effector EseJ in Edwardsiella piscicida inhibits PANoptosis in macrophages
www.nature.com/articles/s42...
www.nature.com/articles/s42...
T3SS effector EseJ in Edwardsiella piscicida inhibits PANoptosis in macrophages - Communications Biology
Edwardsiella piscicida T3SS effector EseJ inhibits PANoptosis (pyroptosis, apoptosis and necroptosis) in murine macrophages by activating TAK1 and disrupting the formation of NLRP3-caspase-8-RIPK1 PANoptosome, thereby enhancing bacterial virulence.
www.nature.com
October 3, 2025 at 3:24 PM
T3SS effector EseJ in Edwardsiella piscicida inhibits PANoptosis in macrophages
www.nature.com/articles/s42...
www.nature.com/articles/s42...
Injectisome assembly primes Pseudomonas aeruginosa for Type III secretion
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Injectisome assembly primes Pseudomonas aeruginosa for Type III secretion
Many Gram-negative pathogens, including Pseudomonas aeruginosa , use a Type III Secretion System (T3SS) to intoxicate eukaryotic cells. The T3SS is an important virulence factor linked to increased mo...
www.biorxiv.org
September 30, 2025 at 4:04 PM
Injectisome assembly primes Pseudomonas aeruginosa for Type III secretion
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by T3SS
First, first-author publication from undergrad Tabitha Cowell is live!
She did some very cool comparisons of putative conjugative T4SS gene clusters and found neat eco evolutionary differences between the wilt pathogen and more environmental species!
I'll make an explainer sometime this week.
She did some very cool comparisons of putative conjugative T4SS gene clusters and found neat eco evolutionary differences between the wilt pathogen and more environmental species!
I'll make an explainer sometime this week.
Lifestyle-associated variation in type IV secretion systems between phytopathogenic and environmental Ralstonia https://www.biorxiv.org/content/10.1101/2025.09.26.675681v1
September 29, 2025 at 12:42 AM
First, first-author publication from undergrad Tabitha Cowell is live!
She did some very cool comparisons of putative conjugative T4SS gene clusters and found neat eco evolutionary differences between the wilt pathogen and more environmental species!
I'll make an explainer sometime this week.
She did some very cool comparisons of putative conjugative T4SS gene clusters and found neat eco evolutionary differences between the wilt pathogen and more environmental species!
I'll make an explainer sometime this week.
Reposted by T3SS
📢A new effector-triggered immunity role for GBP1! No place for pathogens to hide now that GBP1 can detect extracellular pathogens that hijack the actin cytoskeleton. Surprise*suprise* GBP1 does this independently of LPS! 🧵
biorxiv.org/content/10.1...
biorxiv.org/content/10.1...
September 28, 2025 at 10:36 AM
📢A new effector-triggered immunity role for GBP1! No place for pathogens to hide now that GBP1 can detect extracellular pathogens that hijack the actin cytoskeleton. Surprise*suprise* GBP1 does this independently of LPS! 🧵
biorxiv.org/content/10.1...
biorxiv.org/content/10.1...
Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisome | mBio journals.asm.org/doi/10.1128/...
Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisome | mBio
Bordetella bronchiseptica and Bordetella pertussis are two closely related respiratory pathogens that employ their T3SS injectisome
to deliver the BteA effector into host cells. In this study, we visu...
journals.asm.org
September 25, 2025 at 3:59 PM
Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisome | mBio journals.asm.org/doi/10.1128/...
Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion: Cell Reports www.cell.com/cell-reports...
Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion
Host mucins and synthetic glycopolypeptides block Salmonella virulence via glycan-mediated
suppression of Salmonella pathogenicity island 1 (SPI-1). In this study, Wheeler et
al. reveal that gastroint...
www.cell.com
September 24, 2025 at 2:34 PM
Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion: Cell Reports www.cell.com/cell-reports...
Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system | PNAS www.pnas.org/doi/10.1073/...
Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system | PNAS
CRISPR-associated (Cas) endonucleases and their derivatives are widespread tools for
the targeted genetic modification of both prokaryotic and euka...
www.pnas.org
September 21, 2025 at 11:23 PM
Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system | PNAS www.pnas.org/doi/10.1073/...
Structure-Based Identification of Salvianolic Acid B as an Inhibitor Targeting Salmonella InvC ATPase - Journal of Biological Chemistry www.jbc.org/article/S002...
Structure-Based Identification of Salvianolic Acid B as an Inhibitor Targeting Salmonella InvC ATPase
Multidrug-resistant (MDR) Salmonella poses a significant global health threat. The
Type III Secretion System 1 (T3SS-1) and its ATPase InvC are crucial for virulence
and promising drug targets. Throug...
www.jbc.org
September 21, 2025 at 11:23 PM
Structure-Based Identification of Salvianolic Acid B as an Inhibitor Targeting Salmonella InvC ATPase - Journal of Biological Chemistry www.jbc.org/article/S002...