After endocytosis, the acidification of the vesicle lumen acts as a signal for Botox to adopt It only adopt translocation-competent state and to enter the host cytosol.

The Botox in free state is highly dynamic, but upon binding to SV2B receptor, it attains an open conformation, preventing premature entry into the host cell.

Want to know “How does #Botox enter cells at the right time & place to exert its #therapeutic effect?” Check out our latest paper @naturecomms.bsky.social @psich_en @KorkhovLab @ethzurich.bsky.social
www.nature.com/articles/s41...