Tinashe Prince Maviza
@princemaviza.bsky.social
Transcription-Translation enthusiast! Enjoy the thrill of discoveries hidden in nature…
Reposted by Tinashe Prince Maviza
Reposted by Tinashe Prince Maviza
Here we unexpectedly find that the ribosomal protein S20/L26—identified at the dawn of ribosome research but later deemed a biochemical artifact—is indeed a genuine component of the large ribosomal subunit. More on this ribosome content variation is here: www.nature.com/articles/s41...
Structurally heterogeneous ribosomes cooperate in protein synthesis in bacterial cells - Nature Communications
Cells can simultaneously produce structurally dissimilar ribosomes, suggesting functional specialization of distinct ribosome populations. Here, the authors show that distinct ribosomes cooperate rath...
www.nature.com
March 20, 2025 at 10:05 PM
Here we unexpectedly find that the ribosomal protein S20/L26—identified at the dawn of ribosome research but later deemed a biochemical artifact—is indeed a genuine component of the large ribosomal subunit. More on this ribosome content variation is here: www.nature.com/articles/s41...
Reposted by Tinashe Prince Maviza
Latest work from the lab - we identify a translation factor, YebC2, that reduces ribosome stalling in B. subtilis!
Congrats to co-first authors: grad students Hye-Rim Hong & Cassidy Prince & undergrad Dennis Wu @denniswu02.bsky.social
journals.plos.org/plosgenetics...
Congrats to co-first authors: grad students Hye-Rim Hong & Cassidy Prince & undergrad Dennis Wu @denniswu02.bsky.social
journals.plos.org/plosgenetics...
YebC2 resolves ribosome stalling and increases fitness of cells lacking EF-P and the ABCF ATPase YfmR
Author summary Polyproline motifs are essential structural features of many proteins but are difficult for the ribosome to synthesize. EF-P reduces ribosome pausing at polyproline motifs. Here, we sho...
journals.plos.org
April 13, 2025 at 3:11 PM
Latest work from the lab - we identify a translation factor, YebC2, that reduces ribosome stalling in B. subtilis!
Congrats to co-first authors: grad students Hye-Rim Hong & Cassidy Prince & undergrad Dennis Wu @denniswu02.bsky.social
journals.plos.org/plosgenetics...
Congrats to co-first authors: grad students Hye-Rim Hong & Cassidy Prince & undergrad Dennis Wu @denniswu02.bsky.social
journals.plos.org/plosgenetics...
Reposted by Tinashe Prince Maviza
Reposted by Tinashe Prince Maviza
🚨New paper!🚨
A comprehensive take on the origins and evolution of translation factors & how these essential players evolved across the tree of life. 🌍🧬
Led by Evrim Fer @uwmadisonmdtp.bsky.social grad student! 👏
Free access: www.sciencedirect.com/science/arti... @cp-trendsgenetics.bsky.social
A comprehensive take on the origins and evolution of translation factors & how these essential players evolved across the tree of life. 🌍🧬
Led by Evrim Fer @uwmadisonmdtp.bsky.social grad student! 👏
Free access: www.sciencedirect.com/science/arti... @cp-trendsgenetics.bsky.social
March 24, 2025 at 2:46 PM
🚨New paper!🚨
A comprehensive take on the origins and evolution of translation factors & how these essential players evolved across the tree of life. 🌍🧬
Led by Evrim Fer @uwmadisonmdtp.bsky.social grad student! 👏
Free access: www.sciencedirect.com/science/arti... @cp-trendsgenetics.bsky.social
A comprehensive take on the origins and evolution of translation factors & how these essential players evolved across the tree of life. 🌍🧬
Led by Evrim Fer @uwmadisonmdtp.bsky.social grad student! 👏
Free access: www.sciencedirect.com/science/arti... @cp-trendsgenetics.bsky.social
Reposted by Tinashe Prince Maviza
New cryo-EM/ET story - a brilliant collaboration with Sergey Melnikov's lab in Newcastle @sergeymelnikov.bsky.social and Stefan Pfeffer's lab in Heidelberg @pfeffercryolab.bsky.social. Great work in particular by Karla Helena-Bueno and @sophiekopetschke.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structurally heterogeneous ribosomes cooperate in protein synthesis in bacterial cells - Nature Communications
Cells can simultaneously produce structurally dissimilar ribosomes, suggesting functional specialization of distinct ribosome populations. Here, the authors show that distinct ribosomes cooperate rath...
www.nature.com
March 21, 2025 at 9:30 AM
New cryo-EM/ET story - a brilliant collaboration with Sergey Melnikov's lab in Newcastle @sergeymelnikov.bsky.social and Stefan Pfeffer's lab in Heidelberg @pfeffercryolab.bsky.social. Great work in particular by Karla Helena-Bueno and @sophiekopetschke.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tinashe Prince Maviza
This is the third paper from the lab published in the past week. Each of these studies took years to accomplish, and in a nice coincidence all three were published together
The complexity of the bacterial immune system still surprises us, many more discoveries to come
The complexity of the bacterial immune system still surprises us, many more discoveries to come
January 30, 2025 at 9:57 PM
This is the third paper from the lab published in the past week. Each of these studies took years to accomplish, and in a nice coincidence all three were published together
The complexity of the bacterial immune system still surprises us, many more discoveries to come
The complexity of the bacterial immune system still surprises us, many more discoveries to come
Reposted by Tinashe Prince Maviza
Our paper is finally published in Science! 🎉The HPLC, NMR, MS, purification, and characterization of the new molecule felt like a real detective story. I was especially thrilled to use and reference my grandfather, Lev Osterman's, 1966 paper—he was the main reason I became a scientist.
January 31, 2025 at 7:00 AM
Our paper is finally published in Science! 🎉The HPLC, NMR, MS, purification, and characterization of the new molecule felt like a real detective story. I was especially thrilled to use and reference my grandfather, Lev Osterman's, 1966 paper—he was the main reason I became a scientist.
Reposted by Tinashe Prince Maviza
Exciting to see our paper now published 🙌
www.science.org/doi/10.1126/...
In this project co-led with @ostermanilya.bsky.social at @soreklab.bsky.social , we show that a bacterial immune system employs a TIR protein and a caspase-like protease, two typical immune components seen in eukaryotes. 🧵👇
www.science.org/doi/10.1126/...
In this project co-led with @ostermanilya.bsky.social at @soreklab.bsky.social , we show that a bacterial immune system employs a TIR protein and a caspase-like protease, two typical immune components seen in eukaryotes. 🧵👇
January 31, 2025 at 11:41 AM
Exciting to see our paper now published 🙌
www.science.org/doi/10.1126/...
In this project co-led with @ostermanilya.bsky.social at @soreklab.bsky.social , we show that a bacterial immune system employs a TIR protein and a caspase-like protease, two typical immune components seen in eukaryotes. 🧵👇
www.science.org/doi/10.1126/...
In this project co-led with @ostermanilya.bsky.social at @soreklab.bsky.social , we show that a bacterial immune system employs a TIR protein and a caspase-like protease, two typical immune components seen in eukaryotes. 🧵👇
Reposted by Tinashe Prince Maviza
Our paper out @Nature: CARD domains mediate anti-phage defense in bacterial gasdermin systems
CARDs are essential for caspase recruitment during human inflammasome activation. We now find them in bacterial immune systems
Congrats Tana Wein! Thank you Kranzusch lab!
www.nature.com/articles/s41...
CARDs are essential for caspase recruitment during human inflammasome activation. We now find them in bacterial immune systems
Congrats Tana Wein! Thank you Kranzusch lab!
www.nature.com/articles/s41...
January 30, 2025 at 3:40 AM
Our paper out @Nature: CARD domains mediate anti-phage defense in bacterial gasdermin systems
CARDs are essential for caspase recruitment during human inflammasome activation. We now find them in bacterial immune systems
Congrats Tana Wein! Thank you Kranzusch lab!
www.nature.com/articles/s41...
CARDs are essential for caspase recruitment during human inflammasome activation. We now find them in bacterial immune systems
Congrats Tana Wein! Thank you Kranzusch lab!
www.nature.com/articles/s41...
Reposted by Tinashe Prince Maviza
📣 Final version of our @elife.bsky.social article is now online! We show how the human tRNA methyltransferase TRMT1 is recognized, cleaved, and inactivated by the SARS-CoV-2 main protease to disrupt host tRNA modification!
elifesciences.org/articles/91168
A short bluetorial... 🧵 1/9
elifesciences.org/articles/91168
A short bluetorial... 🧵 1/9
Recognition and cleavage of human tRNA methyltransferase TRMT1 by the SARS-CoV-2 main protease
The SARS-CoV-2 main protease specifically cleaves a conserved sequence in the human tRNA modifying enzyme TRMT1, resulting in reduced tRNA binding and the complete loss of TRMT1-mediated tRNA methyltr...
elifesciences.org
January 9, 2025 at 2:36 PM
📣 Final version of our @elife.bsky.social article is now online! We show how the human tRNA methyltransferase TRMT1 is recognized, cleaved, and inactivated by the SARS-CoV-2 main protease to disrupt host tRNA modification!
elifesciences.org/articles/91168
A short bluetorial... 🧵 1/9
elifesciences.org/articles/91168
A short bluetorial... 🧵 1/9
Reposted by Tinashe Prince Maviza
Rescue of stalled (damaged/intact) ribosomes by the peptide release factor PrfH (part of the PrfH-RtcB rescue-repair system). 🧪 #Ribosome doi.org/10.1101/2025...
Molecular and structural basis of a subfamily of PrfH rescuing both the damaged and intact ribosomes stalled in translation
In bacteria, spontaneous mRNAs degradation and ribotoxin-induced RNA damage are two main biological events that lead to the stall of protein translation. The ubiquitous trans-translation system as wel...
doi.org
January 24, 2025 at 3:23 AM
Rescue of stalled (damaged/intact) ribosomes by the peptide release factor PrfH (part of the PrfH-RtcB rescue-repair system). 🧪 #Ribosome doi.org/10.1101/2025...
Reposted by Tinashe Prince Maviza
Our paper out @CellCellPress: Structure guided discovery of viral proteins that inhibit host immunity
Congrats Erez Yirmiya, Azita Leavitt, Gil Amitai, our collaborators at the Kranzusch lab, and coauthors
A 🧵 1/10
www.cell.com/cell/abstrac...
Congrats Erez Yirmiya, Azita Leavitt, Gil Amitai, our collaborators at the Kranzusch lab, and coauthors
A 🧵 1/10
www.cell.com/cell/abstrac...
Structure-guided discovery of viral proteins that inhibit host immunity
Large-scale, structure-guided computational pipeline sifts through millions of phage
proteins to identify those that bind and antagonize host immune proteins. Detected
proteins are shown to inhibit no...
www.cell.com
January 23, 2025 at 4:12 PM
Our paper out @CellCellPress: Structure guided discovery of viral proteins that inhibit host immunity
Congrats Erez Yirmiya, Azita Leavitt, Gil Amitai, our collaborators at the Kranzusch lab, and coauthors
A 🧵 1/10
www.cell.com/cell/abstrac...
Congrats Erez Yirmiya, Azita Leavitt, Gil Amitai, our collaborators at the Kranzusch lab, and coauthors
A 🧵 1/10
www.cell.com/cell/abstrac...
Reposted by Tinashe Prince Maviza
www.sciencedirect.com/science/arti...
Yet another phage defense system that detects free DNA ends and provides immunity without causing 'programmed cell death'.
Yet another phage defense system that detects free DNA ends and provides immunity without causing 'programmed cell death'.
DNA end sensing and cleavage by the Shedu anti-phage defense system
The detection of molecular patterns associated with invading pathogens is a hallmark of innate immune systems. Prokaryotes deploy sophisticated host d…
www.sciencedirect.com
January 1, 2025 at 1:49 PM
www.sciencedirect.com/science/arti...
Yet another phage defense system that detects free DNA ends and provides immunity without causing 'programmed cell death'.
Yet another phage defense system that detects free DNA ends and provides immunity without causing 'programmed cell death'.
Reposted by Tinashe Prince Maviza
Do you know where transcription of a gene actually stops? Hint: it is not the annotated gene end.
In our new paper in Nucleic Acids Research we argue that phosphorylation on threonine 4 of RNA Pol II CTD is a marker of transcription termination in animals... 🧵
academic.oup.com/nar/advance-...
In our new paper in Nucleic Acids Research we argue that phosphorylation on threonine 4 of RNA Pol II CTD is a marker of transcription termination in animals... 🧵
academic.oup.com/nar/advance-...
December 27, 2024 at 4:01 PM
Do you know where transcription of a gene actually stops? Hint: it is not the annotated gene end.
In our new paper in Nucleic Acids Research we argue that phosphorylation on threonine 4 of RNA Pol II CTD is a marker of transcription termination in animals... 🧵
academic.oup.com/nar/advance-...
In our new paper in Nucleic Acids Research we argue that phosphorylation on threonine 4 of RNA Pol II CTD is a marker of transcription termination in animals... 🧵
academic.oup.com/nar/advance-...
Reposted by Tinashe Prince Maviza
Doing good science is 90% finding a science buddy to constantly talk to about the project.
November 9, 2024 at 10:53 PM
Doing good science is 90% finding a science buddy to constantly talk to about the project.
Reposted by Tinashe Prince Maviza
sunburn is from ribosomes stalled by UV damage to RNA? super cool www.sciencedirect.com/science/arti...
The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo
Solar UVB light causes damage to the outermost layer of skin. This insult induces rapid local responses, such as dermal inflammation, keratinocyte cel…
www.sciencedirect.com
November 27, 2024 at 1:22 AM
sunburn is from ribosomes stalled by UV damage to RNA? super cool www.sciencedirect.com/science/arti...
Reposted by Tinashe Prince Maviza
Natural variation in the drug-binding residues of bacterial ribosomes
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Natural variation in the drug-binding residues of bacterial ribosomes
Drugs that target bacterial ribosomes are widely used in modern medicine and veterinary practice to treat bacterial infections and prevent the spread of antimicrobial resistance. However, most studies...
www.biorxiv.org
November 26, 2024 at 11:55 PM
Natural variation in the drug-binding residues of bacterial ribosomes
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Tinashe Prince Maviza
Yeast ribosomes adapt to glucose starvation by tethering to mitochondria in hibernation. Prolonged depletion halts protein synthesis and remodels ribosomes to create protective ribosome arrays. #ribosomes #RNASky #RNABiology #translation rdcu.be/d1xqj
Ribosomes hibernate on mitochondria during cellular stress
Nature Communications - Cells adapt to low glucose by halting protein synthesis and altering organelle shape. Here the authors showed that hibernating ribosomes tether to mitochondria and form...
rdcu.be
November 26, 2024 at 4:21 PM
Yeast ribosomes adapt to glucose starvation by tethering to mitochondria in hibernation. Prolonged depletion halts protein synthesis and remodels ribosomes to create protective ribosome arrays. #ribosomes #RNASky #RNABiology #translation rdcu.be/d1xqj
Reposted by Tinashe Prince Maviza
The Ban lab shows us the #cryoEM structure of the mammalian SRP-SR pre-handover complex on the ribosome. Congratulations to Kan Kobayashi and Ahmad @a__jomaa! http://science.sciencemag.org/content/early/2018/03/21/science.aar7924.full
November 23, 2024 at 1:40 AM
The Ban lab shows us the #cryoEM structure of the mammalian SRP-SR pre-handover complex on the ribosome. Congratulations to Kan Kobayashi and Ahmad @a__jomaa! http://science.sciencemag.org/content/early/2018/03/21/science.aar7924.full
Reposted by Tinashe Prince Maviza
As a biochemist, I am continually surprised that people keep rediscovering that tags affect protein properties
The dark side of fluorescent protein tagging: the impact of protein tags on biomolecular condensation
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 26, 2024 at 12:06 PM
As a biochemist, I am continually surprised that people keep rediscovering that tags affect protein properties
Reposted by Tinashe Prince Maviza
Wonderful tools for Ribosome profiling (Ribo-seq) within a web browser. Check out riboseq.org and academic.oup.com/nar/advance-... 🧪 #RNASky #ribosome
RiboSeq.Org
riboseq.org
November 23, 2024 at 12:23 AM
Wonderful tools for Ribosome profiling (Ribo-seq) within a web browser. Check out riboseq.org and academic.oup.com/nar/advance-... 🧪 #RNASky #ribosome
Reposted by Tinashe Prince Maviza
Faster and more accurate protein modeling with CryFold. By focusing on nearby points (Local Attention) and employing map masking, CryFold excels in generating models from challenging maps regions. It enables a more cost-efficient model building. 🧵
💾 github.com/SBQ-1999/Cry...
💾 github.com/SBQ-1999/Cry...
November 25, 2024 at 5:25 PM
Faster and more accurate protein modeling with CryFold. By focusing on nearby points (Local Attention) and employing map masking, CryFold excels in generating models from challenging maps regions. It enables a more cost-efficient model building. 🧵
💾 github.com/SBQ-1999/Cry...
💾 github.com/SBQ-1999/Cry...