Melvin Noe Gonzalez
@melv-noe-gonzalez.bsky.social
Husband. Father. Postdoc @ Svejstrup lab in Copenhagen studying transcription and DNA damage | EMBO & MSCA fellow
Jarocho de corazón, biochemist by training 🇲🇽
Jarocho de corazón, biochemist by training 🇲🇽
Reposted by Melvin Noe Gonzalez
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Enhancer-promoter compatibility is mediated by the promoter-proximal region
Gene promoters induce transcription in response to distal enhancers. How enhancers specifically activate their target promoter while bypassing other promoters remains unclear. Here, we find that the p...
www.biorxiv.org
October 16, 2025 at 3:06 PM
What is a promoter? And how does it work?
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
We very happy to share our latest work trying to understand enhancer-promoter compatibility.
I am very excited about the results of @blanka-majchrzycka.bsky.social, which changed the way I think about promoters
www.biorxiv.org/content/10.1...
Reposted by Melvin Noe Gonzalez
Thrilled to share our work on transcription initiation and termination being spatially coordinated out today in Science! www.science.org/doi/10.1126/...
mRNA initiation and termination are spatially coordinated
Transcriptional initiation and termination decisions drive messenger RNA (mRNA) isoform diversity but the relationship between them remains poorly understood. By systematically profiling joint usage o...
www.science.org
October 10, 2025 at 6:14 PM
Thrilled to share our work on transcription initiation and termination being spatially coordinated out today in Science! www.science.org/doi/10.1126/...
Reposted by Melvin Noe Gonzalez
🌟 Check out the latest preprint from our center 🌟
This work brings us one step closer to gaining a deeper understanding of gene expression regulation - a core mission of our center 🧬 🧫
If you want to know more about CGEN visit our website: cgen.ku.dk
@dg.dk @ucph.bsky.social
This work brings us one step closer to gaining a deeper understanding of gene expression regulation - a core mission of our center 🧬 🧫
If you want to know more about CGEN visit our website: cgen.ku.dk
@dg.dk @ucph.bsky.social
September 19, 2025 at 12:32 PM
🌟 Check out the latest preprint from our center 🌟
This work brings us one step closer to gaining a deeper understanding of gene expression regulation - a core mission of our center 🧬 🧫
If you want to know more about CGEN visit our website: cgen.ku.dk
@dg.dk @ucph.bsky.social
This work brings us one step closer to gaining a deeper understanding of gene expression regulation - a core mission of our center 🧬 🧫
If you want to know more about CGEN visit our website: cgen.ku.dk
@dg.dk @ucph.bsky.social
Reposted by Melvin Noe Gonzalez
Extremely proud to share the impressive work done by Anouk Olthof et al. on how cells maintain healthy levels of RNAPII, and how they respond when levels are too low. Fascinating stuff, we think www.biorxiv.org/content/10.1...
Systems-level feedback loops maintain gene expression homeostasis following RNA polymerase II dosage perturbation
Transcription is regulated by sequence-specific transcription factors and enzymes allowing access to genes in chromatin. However, recent data indicate that the abundance of RNA polymerase II (RNAPII) ...
www.biorxiv.org
September 19, 2025 at 10:55 AM
Extremely proud to share the impressive work done by Anouk Olthof et al. on how cells maintain healthy levels of RNAPII, and how they respond when levels are too low. Fascinating stuff, we think www.biorxiv.org/content/10.1...
It is my pleasure to share with you the latest from @jsvejstrup.bsky.social lab, where we look at how the reduction of RNAPII levels has a severe, yet organized transcriptional response in the cell.
September 19, 2025 at 9:15 AM
It is my pleasure to share with you the latest from @jsvejstrup.bsky.social lab, where we look at how the reduction of RNAPII levels has a severe, yet organized transcriptional response in the cell.
Reposted by Melvin Noe Gonzalez
Registration and abstract submission is open for the 10th annual Danish RNA Society meeting 🇩🇰 - this year in Aarhus!
Deadline is October 1st
danish-rna-society.nemtilmeld.dk/8/
Deadline is October 1st
danish-rna-society.nemtilmeld.dk/8/
September 10, 2025 at 6:15 PM
Registration and abstract submission is open for the 10th annual Danish RNA Society meeting 🇩🇰 - this year in Aarhus!
Deadline is October 1st
danish-rna-society.nemtilmeld.dk/8/
Deadline is October 1st
danish-rna-society.nemtilmeld.dk/8/
Reposted by Melvin Noe Gonzalez
If you like transcription regulation and its mechanisms, this WIP list is for you.
I'm sure I've forgotten lots of people, so don't hesitate to let me know so I can add you to the list.
go.bsky.app/8vTgeXB
I'm sure I've forgotten lots of people, so don't hesitate to let me know so I can add you to the list.
go.bsky.app/8vTgeXB
August 28, 2025 at 12:48 PM
If you like transcription regulation and its mechanisms, this WIP list is for you.
I'm sure I've forgotten lots of people, so don't hesitate to let me know so I can add you to the list.
go.bsky.app/8vTgeXB
I'm sure I've forgotten lots of people, so don't hesitate to let me know so I can add you to the list.
go.bsky.app/8vTgeXB
Reposted by Melvin Noe Gonzalez
Reposted by Melvin Noe Gonzalez
Excited to share that our latest story on Restrictor is out now in @genesdev.bsky.social!! Big thank you to my fellow co-first author Hanneke, Karen @adelmanlab.bsky.social and co-authors Nathalie and Allison!
In this study, Mimoso et al. show that Restrictor serves as a transcriptional safeguard that suppresses the spurious transcription of non-coding RNAs, while preserving mRNA transcription.
Learn more here:
➡️ tinyurl.com/gd352654
Learn more here:
➡️ tinyurl.com/gd352654
July 14, 2025 at 2:57 PM
Excited to share that our latest story on Restrictor is out now in @genesdev.bsky.social!! Big thank you to my fellow co-first author Hanneke, Karen @adelmanlab.bsky.social and co-authors Nathalie and Allison!
Reposted by Melvin Noe Gonzalez
When sun hits you, what does it *hit*? It rockets into your RNA, and the sound of colliding ribosomes and potholed RNA signals to your body to do something: produce a sunburn. My latest for @quantamagazine.bsky.social.
www.quantamagazine.org/rna-is-the-c...
www.quantamagazine.org/rna-is-the-c...
RNA Is the Cell’s Emergency Alert System | Quanta Magazine
How does a cell know when it’s been damaged? A molecular alarm, set off by mutated RNA and colliding ribosomes, signals danger.
www.quantamagazine.org
July 14, 2025 at 4:27 PM
When sun hits you, what does it *hit*? It rockets into your RNA, and the sound of colliding ribosomes and potholed RNA signals to your body to do something: produce a sunburn. My latest for @quantamagazine.bsky.social.
www.quantamagazine.org/rna-is-the-c...
www.quantamagazine.org/rna-is-the-c...
Reposted by Melvin Noe Gonzalez
Very important comment for the community! This summarizes essential controls to use when performing ChIP-seq and to check when reviewing.
Thank you to the authors
www.nature.com/articles/s41...
Thank you to the authors
www.nature.com/articles/s41...
The Wild West of spike-in normalization - Nature Biotechnology
The proper use of spike-in normalization in ChIP-seq improves sensitivity for detecting genome-wide changes between conditions, but improper use is common, calling some biological conclusions into que...
www.nature.com
December 23, 2024 at 4:57 PM
Very important comment for the community! This summarizes essential controls to use when performing ChIP-seq and to check when reviewing.
Thank you to the authors
www.nature.com/articles/s41...
Thank you to the authors
www.nature.com/articles/s41...
Reposted by Melvin Noe Gonzalez
Excited to share our preprint reporting a completely new approach to study kinase signaling: ProKAS.
ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications.
We applied it to DDR kinases. Please share.
www.biorxiv.org/content/10.1...
ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications.
We applied it to DDR kinases. Please share.
www.biorxiv.org/content/10.1...
December 17, 2024 at 2:10 PM
Excited to share our preprint reporting a completely new approach to study kinase signaling: ProKAS.
ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications.
We applied it to DDR kinases. Please share.
www.biorxiv.org/content/10.1...
ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications.
We applied it to DDR kinases. Please share.
www.biorxiv.org/content/10.1...
Reposted by Melvin Noe Gonzalez
We just posted a preprint about a death mechanism that we find truly surprising.
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
Pol II degradation activates cell death independently from the loss of transcription
Pol II-mediated transcription is essential for eukaryotic life. While loss of transcription is thought to be universally lethal, the associated mechanisms promoting cell death are not yet known. Here,...
www.biorxiv.org
December 13, 2024 at 2:17 PM
We just posted a preprint about a death mechanism that we find truly surprising.
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
When you turn off transcription, cells die (duh!)... but did you know this happens due to loss of Pol II itself, not loss of Pol II activity!?!
preprint here and a thread (1/n): www.biorxiv.org/content/10.1...
🧪
Reposted by Melvin Noe Gonzalez
The first paper from our lab is online! A wonderful collaboration with @scottbscience.bsky.social lab. We discover that Pol II is extensively regulated by Ubiquitin at the beginning of the transcription cycle!
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
CRL3ARMC5 ubiquitin ligase and Integrator phosphatase form parallel mechanisms to control early stages of RNA Pol II transcription
Cacioppo, Gillis, Shlamovitz, Zeller, et al. show that CRL3ARMC5 ubiquitylates RNA Pol II at early stages of transcription as part of a major homeostatic RNA Pol II turnover mechanism. CRL3ARMC5 and the Integrator phosphatase act as complementary pathways, monitoring the quantity and quality of RNA Pol II complexes before they are licensed into elongation.
www.cell.com
December 11, 2024 at 5:19 PM
The first paper from our lab is online! A wonderful collaboration with @scottbscience.bsky.social lab. We discover that Pol II is extensively regulated by Ubiquitin at the beginning of the transcription cycle!
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
Reposted by Melvin Noe Gonzalez
🧪🧬 Pre-print of our CUT&RUN protocol is out NOW!
This comprehensive #protocol includes all the tips and tricks for assay optimization used by our scientists, making it a key read for kit users and home-brew #CUT&RUN users alike.
What makes it special? (1/n)
www.biorxiv.org/content/10.1...
This comprehensive #protocol includes all the tips and tricks for assay optimization used by our scientists, making it a key read for kit users and home-brew #CUT&RUN users alike.
What makes it special? (1/n)
www.biorxiv.org/content/10.1...
High-efficiency genomic mapping of chromatin-associated targets with CUT&RUN
The precise regulation of chromatin composition is critical to gene expression and cellular identity, and thus a key component in development and disease. Robust assays to study chromatin features, in...
www.biorxiv.org
December 11, 2024 at 6:18 PM
🧪🧬 Pre-print of our CUT&RUN protocol is out NOW!
This comprehensive #protocol includes all the tips and tricks for assay optimization used by our scientists, making it a key read for kit users and home-brew #CUT&RUN users alike.
What makes it special? (1/n)
www.biorxiv.org/content/10.1...
This comprehensive #protocol includes all the tips and tricks for assay optimization used by our scientists, making it a key read for kit users and home-brew #CUT&RUN users alike.
What makes it special? (1/n)
www.biorxiv.org/content/10.1...
Reposted by Melvin Noe Gonzalez
Time for our first post here 🥳 Our latest paper is online 🙌
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo
The acute skin reaction to sunburn, encompassing keratinocyte cell death, inflammation,
and epidermal thickening, has traditionally been ascribed to DNA damage responses.
Using a mouse model deficient...
www.cell.com
November 26, 2024 at 10:02 AM
Time for our first post here 🥳 Our latest paper is online 🙌
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
We show that UV activates the ribotoxic stress response, which causes inflammation, cell death and thickening in skin. Read about a role for the RSR separate from DNA damage signaling in response to sunburn: ✨☀️ www.cell.com/molecular-ce...
Reposted by Melvin Noe Gonzalez
As you make have seen, there is a special issue of JMB on "Controlling Transcription Elongation and Termination: More Than a Means to An End" curated by my colleague here at Pitt Karen Arndt and by Steve Buratowski from Harvard Medical School 🧪🧵 1/ www.sciencedirect.com/science/arti...
Controlling Transcription Elongation and Termination: More Than a Means to An End
www.sciencedirect.com
November 27, 2024 at 3:00 PM
As you make have seen, there is a special issue of JMB on "Controlling Transcription Elongation and Termination: More Than a Means to An End" curated by my colleague here at Pitt Karen Arndt and by Steve Buratowski from Harvard Medical School 🧪🧵 1/ www.sciencedirect.com/science/arti...
Reposted by Melvin Noe Gonzalez
Very excited that our paper describing a critical role for the PNUTS-PP1 phosphatase complex in transcription pause release is up online! This was a massive team effort with @edimitrova.bsky.social and the rest of the @robklose.bsky.social lab www.cell.com/molecular-ce... A short thread: (1/8)
The PNUTS phosphatase complex controls transcription pause release
Kelley et al. discover that the PNUTS phosphatase complex plays an essential role
in gene transcription by controlling RNA polymerase II pause release. PNUTS achieves
this through its TND, which recog...
www.cell.com
November 26, 2024 at 4:18 PM
Very excited that our paper describing a critical role for the PNUTS-PP1 phosphatase complex in transcription pause release is up online! This was a massive team effort with @edimitrova.bsky.social and the rest of the @robklose.bsky.social lab www.cell.com/molecular-ce... A short thread: (1/8)
Reposted by Melvin Noe Gonzalez
What happens when you overexpress one subunit of the RNA Pol II? Well, it depends on the subunit, but all deregulate transcription and induce genome instability @agagambus.bsky.social www.biorxiv.org/content/10.1...
RNA Polymerase II subunits overexpressions induce genome instability and deregulate transcription
Independently of the pathways or circuits deregulated in cancer cells, these present altered transcription patterns, often also direct consequence of deregulation of transcription factors. In this sen...
www.biorxiv.org
November 21, 2024 at 10:06 AM
What happens when you overexpress one subunit of the RNA Pol II? Well, it depends on the subunit, but all deregulate transcription and induce genome instability @agagambus.bsky.social www.biorxiv.org/content/10.1...
Reposted by Melvin Noe Gonzalez
This is a very cool paper that I will add to my Mol Bio course. Specific tRNA recruitment of mRNA degradation complex to ribosome through the P site tRNA interactions with CCR4-NOT complex 1/ www.science.org/doi/10.1126/...
Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes
The CCR4-NOT complex is a major regulator of eukaryotic messenger RNA (mRNA) stability. Slow decoding during translation promotes association of CCR4-NOT with ribosomes, accelerating mRNA degradation....
www.science.org
November 21, 2024 at 8:03 PM
This is a very cool paper that I will add to my Mol Bio course. Specific tRNA recruitment of mRNA degradation complex to ribosome through the P site tRNA interactions with CCR4-NOT complex 1/ www.science.org/doi/10.1126/...
Reposted by Melvin Noe Gonzalez
Our latest discovery of “lowest recorded temperature for eukaryotic cell motility” going all the way down to -15deg C. Come for the crazy cold Arctic & stay for diatoms. Will write longer thread - but enjoy video below of diatoms gliding on frozen ice surfaces. Congrats @zqing.bsky.social & team.
November 21, 2024 at 10:36 AM
Our latest discovery of “lowest recorded temperature for eukaryotic cell motility” going all the way down to -15deg C. Come for the crazy cold Arctic & stay for diatoms. Will write longer thread - but enjoy video below of diatoms gliding on frozen ice surfaces. Congrats @zqing.bsky.social & team.
Reposted by Melvin Noe Gonzalez
So excited to share this preprint! The culmination of my postdoctoral works in the Strahl lab and the start of work in my own. In this study, we systematically show PHRF1 to bind the N-terminus of H3 and regulate splicing and DDR. 1/
Histone H3 N-terminal recognition by the PHD finger of PHRF1 is required for proper DNA damage response https://www.biorxiv.org/content/10.1101/2024.11.20.623956v1
Histone H3 N-terminal recognition by the PHD finger of PHRF1 is required for proper DNA damage response https://www.biorxiv.org/content/10.1101/2024.11.20.623956v1
Plant homeodomain (PHD) fingers are critical effectors of histone post-translational modifications (
www.biorxiv.org
November 21, 2024 at 12:30 PM
So excited to share this preprint! The culmination of my postdoctoral works in the Strahl lab and the start of work in my own. In this study, we systematically show PHRF1 to bind the N-terminus of H3 and regulate splicing and DDR. 1/
Reposted by Melvin Noe Gonzalez
Putting together packs of transcription and Chromatin people - Nominate others to add! go.bsky.app/5zgpZfg
November 10, 2024 at 9:43 PM
Putting together packs of transcription and Chromatin people - Nominate others to add! go.bsky.app/5zgpZfg