Chaudhuri Lab
@dipayan.bsky.social
Account of the Chaudhuri lab at the University of Utah
Chaudhurilab.org
Our interests are mitochondrial biology, heart and metabolic diseases, and ion channels.
Chaudhurilab.org
Our interests are mitochondrial biology, heart and metabolic diseases, and ion channels.
Pinned
Chaudhuri Lab
@dipayan.bsky.social
· Nov 27
Mechanism of MCUB-dependent inhibition of mitochondrial calcium uptake https://www.biorxiv.org/content/10.1101/2024.11.25.625228v1
Mechanism of MCUB-dependent inhibition of mitochondrial calcium uptake https://www.biorxiv.org/content/10.1101/2024.11.25.625228v1
Mitochondrial calcium (Ca2+) levels are regulated to balance stimulating respiration against the har
www.biorxiv.org
Recent preprint from our lab describing how MCUB helps inhibit mitochondrial calcium uptake. Will do a longer post with the full publication.
Reposted by Chaudhuri Lab
🚨 Preprint alert! 🚨
My first first-author manuscript is out on bioRxiv!
We developed a new method to monitor mtDNA quality control in yeast, and found that a functional respiratory chain is crucial for the process. @osman-lab.bsky.social
#mtDNA #mitochondria
www.biorxiv.org/content/10.1...
My first first-author manuscript is out on bioRxiv!
We developed a new method to monitor mtDNA quality control in yeast, and found that a functional respiratory chain is crucial for the process. @osman-lab.bsky.social
#mtDNA #mitochondria
www.biorxiv.org/content/10.1...
Local Mitochondrial Physiology Defined by mtDNA Quality Guides Purifying Selection
The mitochondrial genome (mtDNA) encodes essential subunits of the electron transport chain and ATP synthase. Mutations in these genes impair oxidative phosphorylation, compromise mitochondrial ATP pr...
www.biorxiv.org
August 12, 2025 at 7:49 AM
🚨 Preprint alert! 🚨
My first first-author manuscript is out on bioRxiv!
We developed a new method to monitor mtDNA quality control in yeast, and found that a functional respiratory chain is crucial for the process. @osman-lab.bsky.social
#mtDNA #mitochondria
www.biorxiv.org/content/10.1...
My first first-author manuscript is out on bioRxiv!
We developed a new method to monitor mtDNA quality control in yeast, and found that a functional respiratory chain is crucial for the process. @osman-lab.bsky.social
#mtDNA #mitochondria
www.biorxiv.org/content/10.1...
Reposted by Chaudhuri Lab
Meet the 2025 ASCB Award Winners—trailblazing scientists honored for research, mentoring, education, and innovation. Celebrate excellence across all career stages in cell biology. Read more: www.ascb.org/society-news...
August 4, 2025 at 3:52 PM
Meet the 2025 ASCB Award Winners—trailblazing scientists honored for research, mentoring, education, and innovation. Celebrate excellence across all career stages in cell biology. Read more: www.ascb.org/society-news...
Reposted by Chaudhuri Lab
Congrats to the amazing @jiwasa.bsky.social, winner of @ascbiology.bsky.social' Bruce Alberts Award for Excellence in Science Education. Watching her create an innovative career in molecular animation & the impact it has on science and education has been breathtaking.
www.ascb.org/society-news...
www.ascb.org/society-news...
August 5, 2025 at 11:01 AM
Congrats to the amazing @jiwasa.bsky.social, winner of @ascbiology.bsky.social' Bruce Alberts Award for Excellence in Science Education. Watching her create an innovative career in molecular animation & the impact it has on science and education has been breathtaking.
www.ascb.org/society-news...
www.ascb.org/society-news...
Reposted by Chaudhuri Lab
⏰⏰The issue of open access, publishers, and the new NIH rules of no embargo has led to this RFI from NIH. Read this carefully. It has lots of interesting scenarios and is seeking feedback. @briannosek.bsky.social @cos.io thoughts? grants.nih.gov/grants/guide...
NOT-OD-25-138: Request for Information on Maximizing Research Funds by Limiting Allowable Publishing Costs
NIH Funding Opportunities and Notices in the NIH Guide for Grants and Contracts: Request for Information on Maximizing Research Funds by Limiting Allowable Publishing Costs NOT-OD-25-138. NIH
grants.nih.gov
July 30, 2025 at 5:28 PM
⏰⏰The issue of open access, publishers, and the new NIH rules of no embargo has led to this RFI from NIH. Read this carefully. It has lots of interesting scenarios and is seeking feedback. @briannosek.bsky.social @cos.io thoughts? grants.nih.gov/grants/guide...
Reposted by Chaudhuri Lab
And none of this would have been possible without NIH funding.
None of it.
NIH funding was the engine behind this cure as well as just about every other biomedical therapy and cure in modern history.
None of it.
NIH funding was the engine behind this cure as well as just about every other biomedical therapy and cure in modern history.
July 28, 2025 at 1:50 PM
And none of this would have been possible without NIH funding.
None of it.
NIH funding was the engine behind this cure as well as just about every other biomedical therapy and cure in modern history.
None of it.
NIH funding was the engine behind this cure as well as just about every other biomedical therapy and cure in modern history.
Reposted by Chaudhuri Lab
Wes Sundquist is one of the good guys. He is a scientists scientist and has carefully, passionately, and consistently carried his science out with this goal in mind. A cure for AIDS that is available to all.
This is an amazing recognition for him and for the @utah.edu where he has done his work.
This is an amazing recognition for him and for the @utah.edu where he has done his work.
Normalize talking about fed. $ when these headlines happen. Thousands of scientists, working for decades funded by the federal government did this. At times, people thought this was useless research. Below is some info about the HIV labs at the University of Utah. 🧵 www.science.org/doi/10.1126/...
July 28, 2025 at 1:49 PM
Wes Sundquist is one of the good guys. He is a scientists scientist and has carefully, passionately, and consistently carried his science out with this goal in mind. A cure for AIDS that is available to all.
This is an amazing recognition for him and for the @utah.edu where he has done his work.
This is an amazing recognition for him and for the @utah.edu where he has done his work.
Reposted by Chaudhuri Lab
Our story about FASTKD5 is now published at @narjournal.bsky.social It was an amazing collaboration with @haukehillen.bsky.social and Toni Barrientos’ labs.
doi.org/10.1093/nar/...
FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites
doi.org/10.1093/nar/...
FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites
FASTKD5 processes mitochondrial pre-mRNAs at noncanonical cleavage sites
Abstract. The first post-transcriptional step in mammalian mitochondrial gene expression, required for the synthesis of the 13 polypeptides encoded in mito
doi.org
July 11, 2025 at 4:14 AM
Our story about FASTKD5 is now published at @narjournal.bsky.social It was an amazing collaboration with @haukehillen.bsky.social and Toni Barrientos’ labs.
doi.org/10.1093/nar/...
FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites
doi.org/10.1093/nar/...
FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites
Reposted by Chaudhuri Lab
ATTENTION MITO-CALCIUM ENTHUSIASTS: there's a new game in town! This piece highlights a new paper by the Elrod Lab interrogating TMEM65’s role in mitochondrial calcium efflux. The De Stefani and Glancy labs have recently reported similar findings. Congratulations on this important discovery!
NEWS & VIEWS | @yasesancak.bsky.social (U Washington):
A new study shows that the mitochondrial protein TMEM65 has a central role in efflux of calcium ions from the mitochondria.
https://bit.ly/3Sdndzq ?utm_source=bluesky&utm_medium=social&utm_campaign=natmetab
A new study shows that the mitochondrial protein TMEM65 has a central role in efflux of calcium ions from the mitochondria.
https://bit.ly/3Sdndzq ?utm_source=bluesky&utm_medium=social&utm_campaign=natmetab
TMEM65 joins the mitochondrial Ca2+ club - Nature Metabolism
Garbincius et al. show that TMEM65, a mitochondrial transmembrane protein of unknown function, has a central role in efflux of calcium ions from the mitochondria. Mitochondrial calcium efflux is crucial for maintaining mitochondrial calcium levels, which is important for metabolic homeostasis and mitochondrial health.
bit.ly
May 31, 2025 at 5:59 AM
ATTENTION MITO-CALCIUM ENTHUSIASTS: there's a new game in town! This piece highlights a new paper by the Elrod Lab interrogating TMEM65’s role in mitochondrial calcium efflux. The De Stefani and Glancy labs have recently reported similar findings. Congratulations on this important discovery!
Exciting new work by @yasesancak.bsky.social in Science Advancase showing how mitochondrial Ca2 fluxes control branched chain amino acid metabolism. Mitochondrial Ca2 is not just about the TCA cycle!
Our paper on regulation of BCAA catabolism by mitochondrial calcium signaling is out!
Mitochondrial calcium signaling regulates branched-chain amino acid catabolism in fibrolamellar carcinoma | Science Advances www.science.org/doi/10.1126/...
Mitochondrial calcium signaling regulates branched-chain amino acid catabolism in fibrolamellar carcinoma | Science Advances www.science.org/doi/10.1126/...
Mitochondrial calcium signaling regulates branched-chain amino acid catabolism in fibrolamellar carcinoma
Mitochondrial Ca2+ signaling regulates branched-chain amino acid catabolism in an adolescent liver cancer.
www.science.org
June 2, 2025 at 3:19 PM
Exciting new work by @yasesancak.bsky.social in Science Advancase showing how mitochondrial Ca2 fluxes control branched chain amino acid metabolism. Mitochondrial Ca2 is not just about the TCA cycle!
Reposted by Chaudhuri Lab
Huge details are here about compartmentalized metabolic connection. Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis: Molecular Cell www.cell.com/molecular-ce...
Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis
Mitochondria are metabolic hubs that communicate with other organelles via metabolite-,
lipid-, and signaling-molecule exchange facilitated by membrane contact sites (MCSs).
MCSs, regulated by tetheri...
www.cell.com
May 30, 2025 at 3:57 PM
Huge details are here about compartmentalized metabolic connection. Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis: Molecular Cell www.cell.com/molecular-ce...
Reposted by Chaudhuri Lab
Very excited to share our latest work, “Mitochondrial presequences harbor variable strengths to maintain organellar function,” led by the fantastically talented @washubbsb.bsky.social graduate student Youmian Yan. A 🧵
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Mitochondrial presequences harbor variable strengths to maintain organellar function
Hundreds of mitochondrial-destined proteins rely on N-terminal presequences for organellar targeting and import. While generally described as positively charged amphipathic helices, presequences lack ...
www.biorxiv.org
May 29, 2025 at 9:18 PM
Very excited to share our latest work, “Mitochondrial presequences harbor variable strengths to maintain organellar function,” led by the fantastically talented @washubbsb.bsky.social graduate student Youmian Yan. A 🧵
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Chaudhuri Lab
I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
March 21, 2025 at 2:09 PM
I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
Reposted by Chaudhuri Lab
Jiasheng Li, Ye Tian et al. reveal that neuronal mitochondrial stress induces subtle, continuous Ca²⁺ oscillations in a TMBIM-2-dependent manner, enhancing neurotransmission and influencing intertissue mitochondrial stress communication. rupress.org/jcb/article/...
#mitochondria
#mitochondria
March 18, 2025 at 4:50 PM
Jiasheng Li, Ye Tian et al. reveal that neuronal mitochondrial stress induces subtle, continuous Ca²⁺ oscillations in a TMBIM-2-dependent manner, enhancing neurotransmission and influencing intertissue mitochondrial stress communication. rupress.org/jcb/article/...
#mitochondria
#mitochondria
Reposted by Chaudhuri Lab
More Nikon NSPARC confocal testing at the @ncis-marseille.bsky.social: super-resolved live-cell imaging (1 frame every 10 seconds) of a COS-7 cell labeled for DNA (blue), endosomes (pink) and actin (gray) using probes from @spirochrome.com
March 5, 2025 at 3:35 PM
More Nikon NSPARC confocal testing at the @ncis-marseille.bsky.social: super-resolved live-cell imaging (1 frame every 10 seconds) of a COS-7 cell labeled for DNA (blue), endosomes (pink) and actin (gray) using probes from @spirochrome.com
Reposted by Chaudhuri Lab
🙌
New actin probe from @veselin-nasufovic.bsky.social et al! SiR-XActin and its variants are more photostable and less perturbative than SiR-actin or FastAct. And it's already available from @spirochrome.com! With some beautiful data from @chillinwithpfn1.bsky.social:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
February 7, 2025 at 3:19 PM
🙌
Reposted by Chaudhuri Lab
New actin probe from @veselin-nasufovic.bsky.social et al! SiR-XActin and its variants are more photostable and less perturbative than SiR-actin or FastAct. And it's already available from @spirochrome.com! With some beautiful data from @chillinwithpfn1.bsky.social:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
February 6, 2025 at 5:58 PM
New actin probe from @veselin-nasufovic.bsky.social et al! SiR-XActin and its variants are more photostable and less perturbative than SiR-actin or FastAct. And it's already available from @spirochrome.com! With some beautiful data from @chillinwithpfn1.bsky.social:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Chaudhuri Lab
Lars Steinmetz and @seczmarta.bsky.social put together a wonderful perspective on these two studies. www.science.org/doi/10.1126/...
Genome recombination on demand
Large genome rearrangements in mammalian cells can be generated at scale
www.science.org
January 31, 2025 at 1:49 PM
Lars Steinmetz and @seczmarta.bsky.social put together a wonderful perspective on these two studies. www.science.org/doi/10.1126/...
Reposted by Chaudhuri Lab
Woah!
We're delighted to share our work on scrambling the human genome using prime editing, repetitive elements, and recombinases in @science.org , led by @jonaskoeppel.bsky.social , @f-raphael.bsky.social , with @proftomellis.bsky.social and George Church.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Randomizing the human genome by engineering recombination between repeat elements
We lack tools to edit DNA sequences at scales necessary to study 99% of the human genome that is noncoding. To address this gap, we applied CRISPR prime editing to insert recombination handles into re...
www.science.org
January 31, 2025 at 3:30 PM
Woah!
Reposted by Chaudhuri Lab
We're delighted to share our work on scrambling the human genome using prime editing, repetitive elements, and recombinases in @science.org , led by @jonaskoeppel.bsky.social , @f-raphael.bsky.social , with @proftomellis.bsky.social and George Church.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Randomizing the human genome by engineering recombination between repeat elements
We lack tools to edit DNA sequences at scales necessary to study 99% of the human genome that is noncoding. To address this gap, we applied CRISPR prime editing to insert recombination handles into re...
www.science.org
January 31, 2025 at 1:49 PM
We're delighted to share our work on scrambling the human genome using prime editing, repetitive elements, and recombinases in @science.org , led by @jonaskoeppel.bsky.social , @f-raphael.bsky.social , with @proftomellis.bsky.social and George Church.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Chaudhuri Lab
New study from Dave Pagliarini’s lab
Triacylglycerol mobilization underpins mitochondrial stress recovery www.nature.com/articles/s41...
Triacylglycerol mobilization underpins mitochondrial stress recovery www.nature.com/articles/s41...
Triacylglycerol mobilization underpins mitochondrial stress recovery - Nature Cell Biology
Baker et al. show that mitochondrial stress recovery requires mobilization of lipid droplet triacylglycerol stores to facilitate cardiolipin biosynthesis and mitochondrial biogenesis.
www.nature.com
January 9, 2025 at 10:59 AM
New study from Dave Pagliarini’s lab
Triacylglycerol mobilization underpins mitochondrial stress recovery www.nature.com/articles/s41...
Triacylglycerol mobilization underpins mitochondrial stress recovery www.nature.com/articles/s41...
Reposted by Chaudhuri Lab
Mitochondria- and ER-associated actin are required for mitochondrial fusion - Nature Communications
congrats @manorlaboratory.bsky.social & CO
www.nature.com/articles/s41...
congrats @manorlaboratory.bsky.social & CO
www.nature.com/articles/s41...
Mitochondria- and ER-associated actin are required for mitochondrial fusion - Nature Communications
The actin cytoskeleton is crucial for cell and organelle motility. Here, the authors show that actin acts upstream of the mitochondrial fusion machinery to bridge two fusing mitochondria.
www.nature.com
January 9, 2025 at 10:50 AM
Mitochondria- and ER-associated actin are required for mitochondrial fusion - Nature Communications
congrats @manorlaboratory.bsky.social & CO
www.nature.com/articles/s41...
congrats @manorlaboratory.bsky.social & CO
www.nature.com/articles/s41...
Reposted by Chaudhuri Lab
Reposted by Chaudhuri Lab
Congrats to Johannes Linder, David Kelley et al. on the journal publication of Borzoi - a long context sequence models of RNA-seq coverage profiles with many nice applications for transcriptional & post-transcriptional regulation & variant effect prediction.
www.nature.com/articles/s41... 1/
www.nature.com/articles/s41... 1/
Predicting RNA-seq coverage from DNA sequence as a unifying model of gene regulation - Nature Genetics
Borzoi adapts the Enformer sequence-to-expression model to directly predict RNA-seq coverage, enabling the in-silico analysis of variant effects across multiple layers of gene regulation.
www.nature.com
January 8, 2025 at 10:06 PM
Congrats to Johannes Linder, David Kelley et al. on the journal publication of Borzoi - a long context sequence models of RNA-seq coverage profiles with many nice applications for transcriptional & post-transcriptional regulation & variant effect prediction.
www.nature.com/articles/s41... 1/
www.nature.com/articles/s41... 1/
Reposted by Chaudhuri Lab
Super excited to finally share PANCS-Binders: our group's decade-long quest to accelerate protein binder discovery.
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
1/n
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
1/n
While everyone esle has been concentrating on generative binder design, this group has demonstrated screening libraries in vivo! www.biorxiv.org/content/10.1...
#biosky
#biosky
High-throughput protein binder discovery by rapid in vivo selection
Proteins that selectively bind to a target of interest are foundational components of research pipelines, diagnostics, and therapeutics. Current immunization-based, display-based, and computational ap...
www.biorxiv.org
January 7, 2025 at 8:50 PM
Super excited to finally share PANCS-Binders: our group's decade-long quest to accelerate protein binder discovery.
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
1/n
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
1/n