Jenn Cremins
@creminslab.bsky.social
Laboratory for Chromatin and Spatial Neurobiology
We work at the intersection of chromatin, synapses, and neurobiology to understand how the brain stores memory over long timescales.
creminslab.com
x.com/creminslab
https://videocast.nih.gov/watch=55006
We work at the intersection of chromatin, synapses, and neurobiology to understand how the brain stores memory over long timescales.
creminslab.com
x.com/creminslab
https://videocast.nih.gov/watch=55006
Reposted by Jenn Cremins
Worth a read
'Comparative study of pre- and post-mortem perfusion of fixative for the quality of neuronal tissue preparation'
www.nature.com/articles/s41...
'Comparative study of pre- and post-mortem perfusion of fixative for the quality of neuronal tissue preparation'
www.nature.com/articles/s41...
Comparative study of pre- and post-mortem perfusion of fixative for the quality of neuronal tissue preparation - Lab Animal
This study assesses various methods and timings for perfusion of fixatives to enhance brain histology, addressing ethical dilemmas associated with ante-mortem transcardiac perfusion and the lack of da...
www.nature.com
November 3, 2025 at 1:41 PM
Worth a read
'Comparative study of pre- and post-mortem perfusion of fixative for the quality of neuronal tissue preparation'
www.nature.com/articles/s41...
'Comparative study of pre- and post-mortem perfusion of fixative for the quality of neuronal tissue preparation'
www.nature.com/articles/s41...
Reposted by Jenn Cremins
Some hardcore mechanism, for the aficionados (from Peter Jones @vai.org and Ting-Hai Xu @slu-official.bsky.social labs) @natsmb.nature.com www.nature.com/articles/s41...
Mechanisms of DNMT3A–3L-mediated de novo DNA methylation on chromatin - Nature Structural & Molecular Biology
Yan et al. use cryo-EM to obtain structures that reveal how DNMT3A2 and DNMT3L cooperate to read histone signals and bind chromatin, illustrating a mechanism that controls DNA methylation and shapes e...
www.nature.com
November 4, 2025 at 2:55 PM
Some hardcore mechanism, for the aficionados (from Peter Jones @vai.org and Ting-Hai Xu @slu-official.bsky.social labs) @natsmb.nature.com www.nature.com/articles/s41...
Reposted by Jenn Cremins
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
Reposted by Jenn Cremins
The first rule of the Phillies And The World Series is that We. Do. Not. Talk. About. The. Phillies. And. The. World. Series.
Also, 89% of fans are optimistic? 🤔
#RingTheBell but come on, do it responsibly & honestly 😂
Also, 89% of
#RingTheBell but come on, do it responsibly & honestly 😂
this is too much optimism.
it makes me uncomfortable...perhaps even...pessimistic
www.nytimes.com/athletic/666...
it makes me uncomfortable...perhaps even...pessimistic
www.nytimes.com/athletic/666...
September 26, 2025 at 4:48 PM
The first rule of the Phillies And The World Series is that We. Do. Not. Talk. About. The. Phillies. And. The. World. Series.
Also, 89% of fans are optimistic? 🤔
#RingTheBell but come on, do it responsibly & honestly 😂
Also, 89% of
#RingTheBell but come on, do it responsibly & honestly 😂
Reposted by Jenn Cremins
I still get chills
Meet Mike
*30+ years severe depression
*first hospitalized @ 13y
*20 meds
*3 rounds of ECT
*2 near-fatal suicide attempts
Mike felt joy for the first time in decades after we turned on his new brain pacemaker or PACE
see videos, read paper, follow thread
doi.org/10.31234/osf...
Meet Mike
*30+ years severe depression
*first hospitalized @ 13y
*20 meds
*3 rounds of ECT
*2 near-fatal suicide attempts
Mike felt joy for the first time in decades after we turned on his new brain pacemaker or PACE
see videos, read paper, follow thread
doi.org/10.31234/osf...
August 10, 2025 at 6:23 PM
I still get chills
Meet Mike
*30+ years severe depression
*first hospitalized @ 13y
*20 meds
*3 rounds of ECT
*2 near-fatal suicide attempts
Mike felt joy for the first time in decades after we turned on his new brain pacemaker or PACE
see videos, read paper, follow thread
doi.org/10.31234/osf...
Meet Mike
*30+ years severe depression
*first hospitalized @ 13y
*20 meds
*3 rounds of ECT
*2 near-fatal suicide attempts
Mike felt joy for the first time in decades after we turned on his new brain pacemaker or PACE
see videos, read paper, follow thread
doi.org/10.31234/osf...
Reposted by Jenn Cremins
Tres cool!
A postnatal molecular switch drives activity-dependent maturation of parvalbumin interneurons: Cell www.cell.com/cell/fulltex...
A postnatal molecular switch drives activity-dependent maturation of parvalbumin interneurons: Cell www.cell.com/cell/fulltex...
A postnatal molecular switch drives activity-dependent maturation of parvalbumin interneurons
Neuronal activity triggers PGC-1α-mediated transcriptional programs that drive the
late maturation of PV+ interneurons, essential for cortical circuit function. PGC-1α
partners with ERRγ and Mef2c to ...
www.cell.com
July 16, 2025 at 12:33 PM
Tres cool!
A postnatal molecular switch drives activity-dependent maturation of parvalbumin interneurons: Cell www.cell.com/cell/fulltex...
A postnatal molecular switch drives activity-dependent maturation of parvalbumin interneurons: Cell www.cell.com/cell/fulltex...
Reposted by Jenn Cremins
Hippocampal ensembles regulate circuit-induced relapse of extinguished fear
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Hippocampal ensembles regulate circuit-induced relapse of extinguished fear - Molecular Psychiatry
Molecular Psychiatry - Hippocampal ensembles regulate circuit-induced relapse of extinguished fear
www.nature.com
May 26, 2025 at 10:54 AM
Hippocampal ensembles regulate circuit-induced relapse of extinguished fear
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Jenn Cremins
Molecular Underpinnings of Memory Persistence and Forgetting
doi.org/10.1111/jnc....
doi.org/10.1111/jnc....
Molecular Underpinnings of Memory Persistence and Forgetting
In this review article, we discuss molecular mechanisms underlying memory durability. We highlight neurotransmitters, receptors, intracellular signaling cascades, gene expression, actin dynamics, and...
doi.org
May 26, 2025 at 11:01 AM
Molecular Underpinnings of Memory Persistence and Forgetting
doi.org/10.1111/jnc....
doi.org/10.1111/jnc....
Reposted by Jenn Cremins
Neural activity in the brain when a #zebrafish recognizes food (paramecium). Credit to Dr. Akira Muto. #ZebrafishZunday 🧪
May 18, 2025 at 6:47 AM
Neural activity in the brain when a #zebrafish recognizes food (paramecium). Credit to Dr. Akira Muto. #ZebrafishZunday 🧪
Reposted by Jenn Cremins
Thrilled to share our latest study out in @natureportfolio.nature.com led by the fantastically talented Jing Liu. Our study provides insight into a long standing question in biology: What molecular features make us uniquely human and how do these function? www.nature.com/articles/s41...
A human-specific enhancer fine-tunes radial glia potency and corticogenesis - Nature
HARE5, a human accelerated region enhancer, modulates cortical development by influencing neural progenitor cell behaviour, leading to an enlarged neocortex with increased functional independence betw...
www.nature.com
May 14, 2025 at 4:09 PM
Thrilled to share our latest study out in @natureportfolio.nature.com led by the fantastically talented Jing Liu. Our study provides insight into a long standing question in biology: What molecular features make us uniquely human and how do these function? www.nature.com/articles/s41...
Reposted by Jenn Cremins
🧠 New in Nature from the fabulous Silver Lab @ Duke: A human-specific enhancer, HARE5, boosts radial glia proliferation, expanding neocortical size and connectivity. Knock-in mice showed enlarged cortices and more neurons. This highlights how regulatory DNA changes shaped human brain evolution.
Thrilled to share our latest study out in @natureportfolio.nature.com led by the fantastically talented Jing Liu. Our study provides insight into a long standing question in biology: What molecular features make us uniquely human and how do these function? www.nature.com/articles/s41...
A human-specific enhancer fine-tunes radial glia potency and corticogenesis - Nature
HARE5, a human accelerated region enhancer, modulates cortical development by influencing neural progenitor cell behaviour, leading to an enlarged neocortex with increased functional independence betw...
www.nature.com
May 14, 2025 at 10:33 PM
🧠 New in Nature from the fabulous Silver Lab @ Duke: A human-specific enhancer, HARE5, boosts radial glia proliferation, expanding neocortical size and connectivity. Knock-in mice showed enlarged cortices and more neurons. This highlights how regulatory DNA changes shaped human brain evolution.
Reposted by Jenn Cremins
➡️ Delighted to contribute to this piece on the mechanisms underlying memory reconsolidation and persistence, together with Hugo Bayer, Cristina Stern and @stevemaren.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Windows of change: Revisiting temporal and molecular dynamics of memory reconsolidation and persistenc
Retrieval can bring memories to a labile state, creating a window to modify its content during reconsolidation. Numerous studies have investigated thi…
www.sciencedirect.com
May 11, 2025 at 12:10 PM
➡️ Delighted to contribute to this piece on the mechanisms underlying memory reconsolidation and persistence, together with Hugo Bayer, Cristina Stern and @stevemaren.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Jenn Cremins
I think about this comic at least once a week
May 10, 2025 at 5:47 AM
I think about this comic at least once a week
Reposted by Jenn Cremins
Love this. I'm always puzzled by physiological v pathological abeta, tau, & ptau. This is a cool study looking at physiological v pathological abeta on synapses.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Divergent actions of physiological and pathological amyloid-β on synapses in live human brain slice cultures - Nature Communications
Understanding synapse loss in Alzheimer’s disease has been hampered by a lack of human model systems. Here, the authors show that manipulation of physiological or pathological Aβ has differing effects...
www.nature.com
April 30, 2025 at 12:14 PM
Love this. I'm always puzzled by physiological v pathological abeta, tau, & ptau. This is a cool study looking at physiological v pathological abeta on synapses.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Jenn Cremins
Over 50 yrs since the discovery of protein kinases, 80% of human kinases still have ≤20 known substrates, and many are “dark.” I'm EXCITED to announce our new work towards solving this- combining (1) deep learning with (2) proximity proteomics in vivo! ➡️
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 28, 2025 at 10:00 PM
Over 50 yrs since the discovery of protein kinases, 80% of human kinases still have ≤20 known substrates, and many are “dark.” I'm EXCITED to announce our new work towards solving this- combining (1) deep learning with (2) proximity proteomics in vivo! ➡️
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Jenn Cremins
In-depth investigation of proteomic changes in plaque-associated axonal swellings 😮
Online now! Using subcellular proximity labeling proteomics in human brain and iPSC modeling Yifei Cai & Jaime Grutzendler and colleagues link spheroid formation to dysregulated mTOR, cytoskeletal and lipid transport signaling. (rdcu.be/ecUXQ)
doi.org/10.1038/s435...
doi.org/10.1038/s435...
Subcellular proteomics and iPSC modeling uncover reversible mechanisms of axonal pathology in Alzheimer’s disease
Nature Aging - Axonal spheroids disrupt neural circuits in Alzheimer’s disease. In this study, using subcellular proximity labeling proteomics in human brain and iPSC modeling, the authors...
rdcu.be
March 11, 2025 at 10:24 AM
In-depth investigation of proteomic changes in plaque-associated axonal swellings 😮
transcriptionally similar cells can be functionally diverse
March 2, 2025 at 12:38 PM
transcriptionally similar cells can be functionally diverse
Reposted by Jenn Cremins
I am very proud to announce that my PhD paper finally came out in Cell! In this *very* collaborative study, we develop and release a deep-learning approach to predict neuron type identity from their electrical signature. doi.org/10.1016/j.ce... 1/16 🧵
March 1, 2025 at 1:14 PM
I am very proud to announce that my PhD paper finally came out in Cell! In this *very* collaborative study, we develop and release a deep-learning approach to predict neuron type identity from their electrical signature. doi.org/10.1016/j.ce... 1/16 🧵
#keepNIHstrong
Research is a virtuous cycle that brings hope to those hurting.
Researchers and physicians are front line warriors for the greatest medical problems of our time.
Research fuels engines of innovation and drives economic growth.
www.unitedformedicalresearch.org/annual-econo...
Research is a virtuous cycle that brings hope to those hurting.
Researchers and physicians are front line warriors for the greatest medical problems of our time.
Research fuels engines of innovation and drives economic growth.
www.unitedformedicalresearch.org/annual-econo...
January 23, 2025 at 3:41 PM
#keepNIHstrong
Research is a virtuous cycle that brings hope to those hurting.
Researchers and physicians are front line warriors for the greatest medical problems of our time.
Research fuels engines of innovation and drives economic growth.
www.unitedformedicalresearch.org/annual-econo...
Research is a virtuous cycle that brings hope to those hurting.
Researchers and physicians are front line warriors for the greatest medical problems of our time.
Research fuels engines of innovation and drives economic growth.
www.unitedformedicalresearch.org/annual-econo...
Reposted by Jenn Cremins
Parents matter - important study for modeling amyloid accumulation 🐭
Parental origin of transgene modulates amyloid-β plaque burden in the 5xFAD mouse model of Alzheimer’s disease
The 5xFAD transgenic model, popular in Alzheimer’s disease research, exhibits varying
cerebral plaque burden depending on the parental source of the transgene. Mice inheriting
the transgene paternally...
www.cell.com
January 20, 2025 at 8:50 PM
Parents matter - important study for modeling amyloid accumulation 🐭
Reposted by Jenn Cremins
Spiny night for #FluorescenceFriday! Trying some out of left field ways to visualize busy samples. Actin (rainbow depth-color coded) and microtubules (gray) along mature dendrites. projection of a 3D-SIM stack with some undisclosed tricks
January 10, 2025 at 9:01 PM
Spiny night for #FluorescenceFriday! Trying some out of left field ways to visualize busy samples. Actin (rainbow depth-color coded) and microtubules (gray) along mature dendrites. projection of a 3D-SIM stack with some undisclosed tricks
Reposted by Jenn Cremins
Happy New Year, blue-sci-peeps!
My piece in Annual Review of Biophysics on chromatin replication is now available online.
doi.org/10.1146/annu...
If you have paywall issues, try this link:
madhanilab.ucsf.edu/s/annurev-bi...
My piece in Annual Review of Biophysics on chromatin replication is now available online.
doi.org/10.1146/annu...
If you have paywall issues, try this link:
madhanilab.ucsf.edu/s/annurev-bi...
Mechanisms of Inheritance of Chromatin States: From Yeast to Human | Annual Reviews
In this article I review mechanisms that underpin epigenetic inheritance of CpG methylation and histone H3 lysine 9 methylation (H3K9me) in chromatin in fungi and mammals. CpG methylation can be faith...
doi.org
January 7, 2025 at 1:22 AM
Happy New Year, blue-sci-peeps!
My piece in Annual Review of Biophysics on chromatin replication is now available online.
doi.org/10.1146/annu...
If you have paywall issues, try this link:
madhanilab.ucsf.edu/s/annurev-bi...
My piece in Annual Review of Biophysics on chromatin replication is now available online.
doi.org/10.1146/annu...
If you have paywall issues, try this link:
madhanilab.ucsf.edu/s/annurev-bi...
Reposted by Jenn Cremins
Our mentor Casey Brown, apart from being an extraordinary scientist, was a brilliant teacher who loved everything genetics. In his honor, some of the world's leading experts came together to create the 'Casey Brown Lecture Series' on human genetics 1/ shorturl.at/eCm7S
Casey Brown Lecture Series - YouTube
shorturl.at
January 4, 2025 at 2:54 PM
Our mentor Casey Brown, apart from being an extraordinary scientist, was a brilliant teacher who loved everything genetics. In his honor, some of the world's leading experts came together to create the 'Casey Brown Lecture Series' on human genetics 1/ shorturl.at/eCm7S
crosstalk between mRNA export and transcription
Why can a human tolerate a drug that globally inhibits transcription? Why do transcription inhibitors not cure cancer? Our first paper of 2025 may help explain (some) of this!
So incredibly proud of @tobiaswilliams.bsky.social & Ewa Michalak who led the work!
www.cell.com/molecular-ce...
So incredibly proud of @tobiaswilliams.bsky.social & Ewa Michalak who led the work!
www.cell.com/molecular-ce...
mRNA export factors store nascent transcripts within nuclear speckles as an adaptive response to transient global inhibition of transcription
Transcription inhibitors also disrupt nuclear export. Here, Williams et al. reveal that mRNA export factors sense transcription inhibition and adapt by storing mature export-competent mRNA in nuclear speckles. This enables rapid release when transcription resumes and ensures retention of cellular identity and viability during a transient global transcription insult.
www.cell.com
January 2, 2025 at 10:12 PM
crosstalk between mRNA export and transcription
Reposted by Jenn Cremins
Epigenetic memory that couples 5mC and H3K9 methylation, enables chromatin state inheritance across lifetimes and evolution. New excellent review by @hitenmadhani.bsky.social highlights inheritance mechanisms including roles for histone chaperones and nucleosome recycling. tinyurl.com/42ddhj4a
December 24, 2024 at 2:37 PM
Epigenetic memory that couples 5mC and H3K9 methylation, enables chromatin state inheritance across lifetimes and evolution. New excellent review by @hitenmadhani.bsky.social highlights inheritance mechanisms including roles for histone chaperones and nucleosome recycling. tinyurl.com/42ddhj4a