Jonathan KM Lim
@j0nlim.bsky.social
Cancer biologist at @HHU / University Hospital Düsseldorf 🇩🇪
PhD @ UBC 🇨🇦
Interested in oncogenic RAS signaling, cancer metabolism, lncRNAs
GoogleScholar: https://g.co/kgs/jCXqEPN
Website: https://shorturl.at/IDSGc
PhD @ UBC 🇨🇦
Interested in oncogenic RAS signaling, cancer metabolism, lncRNAs
GoogleScholar: https://g.co/kgs/jCXqEPN
Website: https://shorturl.at/IDSGc
Reposted by Jonathan KM Lim
Since CXADR is a viral receptor, this study further paves the way for our novel virotherapy of #Ewing #sarcoma based on our 2022 study by @hoelting.bsky.social pioneering a suicde gene therapy induced by a Ewing sarcoma-specifc synthetic GGAA-enhancer
tinyurl.com/2sckk89m
tinyurl.com/2sckk89m
Neomorphic DNA-binding enables tumor-specific therapeutic gene expression in fusion-addicted childhood sarcoma - Molecular Cancer
Chimeric fusion transcription factors are oncogenic hallmarks of several devastating cancer entities including pediatric sarcomas, such as Ewing sarcoma (EwS) and alveolar rhabdomyosarcoma (ARMS). Des...
tinyurl.com
August 11, 2025 at 6:41 AM
Since CXADR is a viral receptor, this study further paves the way for our novel virotherapy of #Ewing #sarcoma based on our 2022 study by @hoelting.bsky.social pioneering a suicde gene therapy induced by a Ewing sarcoma-specifc synthetic GGAA-enhancer
tinyurl.com/2sckk89m
tinyurl.com/2sckk89m
Reposted by Jonathan KM Lim
Very proud on our stellar Alina Ritter for her frist first-author paper:
Targeting CXADR-mediated AKT signaling suppresses tumorigenesis and enhances #chemotherapy efficacy in #Ewing #sarcoma
tinyurl.com/3kcs3bc9
@dkfz.bsky.social
@uniheidelberg.bsky.social
@nct-heidelberg.bsky.social
Targeting CXADR-mediated AKT signaling suppresses tumorigenesis and enhances #chemotherapy efficacy in #Ewing #sarcoma
tinyurl.com/3kcs3bc9
@dkfz.bsky.social
@uniheidelberg.bsky.social
@nct-heidelberg.bsky.social
August 11, 2025 at 6:38 AM
Very proud on our stellar Alina Ritter for her frist first-author paper:
Targeting CXADR-mediated AKT signaling suppresses tumorigenesis and enhances #chemotherapy efficacy in #Ewing #sarcoma
tinyurl.com/3kcs3bc9
@dkfz.bsky.social
@uniheidelberg.bsky.social
@nct-heidelberg.bsky.social
Targeting CXADR-mediated AKT signaling suppresses tumorigenesis and enhances #chemotherapy efficacy in #Ewing #sarcoma
tinyurl.com/3kcs3bc9
@dkfz.bsky.social
@uniheidelberg.bsky.social
@nct-heidelberg.bsky.social
Reposted by Jonathan KM Lim
New paper online:
Ångström-resolution imaging of cell-surface glycans.
The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.
#Glycotime
www.nature.com/articles/s41...
Ångström-resolution imaging of cell-surface glycans.
The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.
#Glycotime
www.nature.com/articles/s41...
Ångström-resolution imaging of cell-surface glycans - Nature Nanotechnology
By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...
www.nature.com
July 28, 2025 at 9:44 AM
New paper online:
Ångström-resolution imaging of cell-surface glycans.
The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.
#Glycotime
www.nature.com/articles/s41...
Ångström-resolution imaging of cell-surface glycans.
The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.
#Glycotime
www.nature.com/articles/s41...
Reposted by Jonathan KM Lim
A highly compact chemically induced proximity system dependent on salicylic acid or aspirin enables control of biological processes and cellular therapeutics using an over-the counter drug with minimal side effects
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Repurposing salicylic acid as a versatile inducer of proximity - Nature Chemical Biology
A highly compact chemically induced proximity system dependent on salicylic acid or aspirin enables control of biological processes and cellular therapeutics using an over-the-counter drug with minima...
www.nature.com
June 13, 2025 at 7:36 PM
A highly compact chemically induced proximity system dependent on salicylic acid or aspirin enables control of biological processes and cellular therapeutics using an over-the counter drug with minimal side effects
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Jonathan KM Lim
Synthetic biology could enable new types of programmable therapeutics. Our new preprint introduces synthetic protein circuits that selectively trigger cell death in Ras-mutant cancer cells, with interesting advantages compared to existing approaches.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
June 3, 2025 at 12:11 AM
Synthetic biology could enable new types of programmable therapeutics. Our new preprint introduces synthetic protein circuits that selectively trigger cell death in Ras-mutant cancer cells, with interesting advantages compared to existing approaches.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Jonathan KM Lim
What do cells undergoing #ferroptosis #celldeath release? Read all about it in our atlas on ferroptosis #secretomes out now in Cell Death and Differentiation #CDDpress
Great collaboration with @cecad.bsky.social @frezzalab.bsky.social @kreuzaler.bsky.social @almutschulze.bsky.social
rdcu.be/ejp9C
Great collaboration with @cecad.bsky.social @frezzalab.bsky.social @kreuzaler.bsky.social @almutschulze.bsky.social
rdcu.be/ejp9C
An atlas of ferroptosis-induced secretomes
Cell Death & Differentiation - An atlas of ferroptosis-induced secretomes
rdcu.be
April 26, 2025 at 9:42 AM
What do cells undergoing #ferroptosis #celldeath release? Read all about it in our atlas on ferroptosis #secretomes out now in Cell Death and Differentiation #CDDpress
Great collaboration with @cecad.bsky.social @frezzalab.bsky.social @kreuzaler.bsky.social @almutschulze.bsky.social
rdcu.be/ejp9C
Great collaboration with @cecad.bsky.social @frezzalab.bsky.social @kreuzaler.bsky.social @almutschulze.bsky.social
rdcu.be/ejp9C
Reposted by Jonathan KM Lim
☕Fan et al. report a role for PRMT5 in regulating GPX4 stability and #ferroptosis sensitivity through arginine methylation, which may be targeted to inhibit #tumor development.
rdcu.be/ejc3M
bit.ly/4iyxJMj
rdcu.be/ejc3M
bit.ly/4iyxJMj
PRMT5-mediated arginine methylation stabilizes GPX4 to suppress ferroptosis in cancer - Nature Cell Biology
Fan et al. report a role for PRMT5 in regulating GPX4 stability and ferroptosis sensitivity through arginine methylation, which may be targeted to inhibit tumour development.
bit.ly
April 24, 2025 at 7:29 PM
☕Fan et al. report a role for PRMT5 in regulating GPX4 stability and #ferroptosis sensitivity through arginine methylation, which may be targeted to inhibit #tumor development.
rdcu.be/ejc3M
bit.ly/4iyxJMj
rdcu.be/ejc3M
bit.ly/4iyxJMj
Reposted by Jonathan KM Lim
Targeting PIKfyve-driven lipid metabolism in pancreatic cancer
www.nature.com/articles/s41...
Congrats to the @lyssiotislab.bsky.social and all the others involved
www.nature.com/articles/s41...
Congrats to the @lyssiotislab.bsky.social and all the others involved
Targeting PIKfyve-driven lipid metabolism in pancreatic cancer - Nature
A study of human and mouse models of pancreatic cancer finds that inhibiting the lipid kinase PIKfyve interferes with the cancer’s lipid homeostasis, making it a potential target for drug development.
www.nature.com
April 23, 2025 at 4:09 PM
Targeting PIKfyve-driven lipid metabolism in pancreatic cancer
www.nature.com/articles/s41...
Congrats to the @lyssiotislab.bsky.social and all the others involved
www.nature.com/articles/s41...
Congrats to the @lyssiotislab.bsky.social and all the others involved
Reposted by Jonathan KM Lim
Low overlap of transcription factor DNA binding and regulatory targets @nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
April 16, 2025 at 7:32 PM
Low overlap of transcription factor DNA binding and regulatory targets @nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Jonathan KM Lim
A cell membrane-localized NADH pool, very cool!
ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1 www.cell.com/cell/abstrac...
ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1 www.cell.com/cell/abstrac...
ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1
A membrane-associated pool of NADH confers protection against ferroptosis.
www.cell.com
April 14, 2025 at 3:08 PM
A cell membrane-localized NADH pool, very cool!
ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1 www.cell.com/cell/abstrac...
ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1 www.cell.com/cell/abstrac...
Reposted by Jonathan KM Lim
In 2016, the Kishony Lab at Harvard Medical School put together this demonstration of acquired antibiotic resistance in E. coli bacteria, creating a mega-Petri dish (2' x 4') with a stepped gradient of antibiotic.
Seeing the mutants emerge & spread is an elegant demo of evolutionary change.
Seeing the mutants emerge & spread is an elegant demo of evolutionary change.
The Evolution of Bacteria on a “Mega-Plate” Petri Dish (Kishony Lab)
YouTube video by Harvard Medical School
youtu.be
April 13, 2025 at 2:01 PM
In 2016, the Kishony Lab at Harvard Medical School put together this demonstration of acquired antibiotic resistance in E. coli bacteria, creating a mega-Petri dish (2' x 4') with a stepped gradient of antibiotic.
Seeing the mutants emerge & spread is an elegant demo of evolutionary change.
Seeing the mutants emerge & spread is an elegant demo of evolutionary change.
Reposted by Jonathan KM Lim
☕Ueda et al. develop a sensor targeted to #tumor cells that tracks extracellular H2O2. Tumor cells in H2O2 hotspots undergo partial epithelial–mesenchymal transition and migrate away from H2O2 hotspots. This is not seen in NRF2-hyperactivated tumors.
rdcu.be/eeQwh
bit.ly/4hAJ5it
rdcu.be/eeQwh
bit.ly/4hAJ5it
Intratumour oxidative hotspots provide a niche for cancer cell dissemination - Nature Cell Biology
Ueda et al. develop a sensor targeted to tumour cells that tracks extracellular H2O2. Tumour cells in H2O2 hotspots undergo partial epithelial–mesenchymal transition and migrate away from H2O2 hotspot...
bit.ly
March 24, 2025 at 1:26 PM
☕Ueda et al. develop a sensor targeted to #tumor cells that tracks extracellular H2O2. Tumor cells in H2O2 hotspots undergo partial epithelial–mesenchymal transition and migrate away from H2O2 hotspots. This is not seen in NRF2-hyperactivated tumors.
rdcu.be/eeQwh
bit.ly/4hAJ5it
rdcu.be/eeQwh
bit.ly/4hAJ5it
Reposted by Jonathan KM Lim
Fascinating work from MALDI master Ramon Sun and collaborators on glycogen metabolism in NSCLC. A real tour de force and such beautiful images 😍 www.nature.com/articles/s42...
Glycogen drives tumour initiation and progression in lung adenocarcinoma - Nature Metabolism
Clarke, Hawkinson and colleagues study the contribution of glycogen accumulation in the onset and progression of lung cancer.
www.nature.com
March 11, 2025 at 4:10 PM
Fascinating work from MALDI master Ramon Sun and collaborators on glycogen metabolism in NSCLC. A real tour de force and such beautiful images 😍 www.nature.com/articles/s42...
Reposted by Jonathan KM Lim
Happy 37th birthday to the LTEE!
the-ltee.org/history/
the-ltee.org/history/
History – The Long-Term Evolution Experiment
the-ltee.org
February 24, 2025 at 3:39 PM
Happy 37th birthday to the LTEE!
the-ltee.org/history/
the-ltee.org/history/
Reposted by Jonathan KM Lim
Larger species have a higher cancer prevalence than smaller species, according to a study that refutes Peto’s Paradox, the claim that that species’ body size is not correlated with cancer risk. In PNAS: www.pnas.org/doi/10.1073/...
March 4, 2025 at 4:03 PM
Larger species have a higher cancer prevalence than smaller species, according to a study that refutes Peto’s Paradox, the claim that that species’ body size is not correlated with cancer risk. In PNAS: www.pnas.org/doi/10.1073/...
Tenure track apps are like finding love.
The motivation letter is the love letter. The interview, a fancy date. Youre to find out what they’re into, convince them why you’re compatible, a process wherein you fantasize being with them, moving countries for them. Alas they tell you’re not a good fit.
The motivation letter is the love letter. The interview, a fancy date. Youre to find out what they’re into, convince them why you’re compatible, a process wherein you fantasize being with them, moving countries for them. Alas they tell you’re not a good fit.
February 22, 2025 at 8:48 AM
Tenure track apps are like finding love.
The motivation letter is the love letter. The interview, a fancy date. Youre to find out what they’re into, convince them why you’re compatible, a process wherein you fantasize being with them, moving countries for them. Alas they tell you’re not a good fit.
The motivation letter is the love letter. The interview, a fancy date. Youre to find out what they’re into, convince them why you’re compatible, a process wherein you fantasize being with them, moving countries for them. Alas they tell you’re not a good fit.
Reposted by Jonathan KM Lim
First: I apologize for never coming here and now doing so only to brag 😬
Second: I am very proud of the people in our lab. We are a small group in a Bio department and this work took an enormous amount of effort from every one here.
rdcu.be/eaxEz
Second: I am very proud of the people in our lab. We are a small group in a Bio department and this work took an enormous amount of effort from every one here.
rdcu.be/eaxEz
Cooperative nutrient scavenging is an evolutionary advantage in cancer
Nature - Nutrient-starved tumour cells cooperate by secreting aminopeptidases that digest oligopeptides in the microenvironment, creating a shared pool of free amino acids.
rdcu.be
February 20, 2025 at 4:39 PM
First: I apologize for never coming here and now doing so only to brag 😬
Second: I am very proud of the people in our lab. We are a small group in a Bio department and this work took an enormous amount of effort from every one here.
rdcu.be/eaxEz
Second: I am very proud of the people in our lab. We are a small group in a Bio department and this work took an enormous amount of effort from every one here.
rdcu.be/eaxEz
Reposted by Jonathan KM Lim
The latest chapter in tuneable gene expression system uses
nitroglycerin to enable controlled and reversible production of glucagon-like peptide-1 over 35-days restoring blood glucose levels in mice with #diabetes without affecting heart rate or blood pressure. www.nature.com/articles/s41...
nitroglycerin to enable controlled and reversible production of glucagon-like peptide-1 over 35-days restoring blood glucose levels in mice with #diabetes without affecting heart rate or blood pressure. www.nature.com/articles/s41...
Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins - Nature Biomedical Engineering
Clinically licensed nitroglycerin patches allowed for the on-demand and sustained expression of glucagon-like peptide-1 by human cells engineered with a nitroglycerin-responsive gene switch subcutaneo...
www.nature.com
February 15, 2025 at 11:30 AM
The latest chapter in tuneable gene expression system uses
nitroglycerin to enable controlled and reversible production of glucagon-like peptide-1 over 35-days restoring blood glucose levels in mice with #diabetes without affecting heart rate or blood pressure. www.nature.com/articles/s41...
nitroglycerin to enable controlled and reversible production of glucagon-like peptide-1 over 35-days restoring blood glucose levels in mice with #diabetes without affecting heart rate or blood pressure. www.nature.com/articles/s41...
Reposted by Jonathan KM Lim
Research Briefing: Starving tumors with fat www.nature.com/articles/s41...
Starving tumors with fat - Nature Biotechnology
Adipocytes can be isolated, genetically manipulated, and then reimplanted. In this study, these properties were leveraged to engineer adipocytes that can outcompete tumors for essential metabolic reso...
www.nature.com
February 6, 2025 at 4:27 PM
Research Briefing: Starving tumors with fat www.nature.com/articles/s41...
Reposted by Jonathan KM Lim
GUK1 activation is a metabolic liability in lung cancer: Cell www.cell.com/cell/fulltex...
GUK1 activation is a metabolic liability in lung cancer
Schneider et al. identify guanylate kinase 1 (GUK1), a GDP-synthesizing enzyme, as
a target of oncogenic signaling in lung cancer. Phosphorylation of GUK1 augments nucleotide
pools and is important fo...
www.cell.com
February 6, 2025 at 4:53 PM
GUK1 activation is a metabolic liability in lung cancer: Cell www.cell.com/cell/fulltex...
Reposted by Jonathan KM Lim
Research Highlight: Therapeutic T cells with synthetic gene circuits act locally www.nature.com/articles/s41...
Therapeutic T cells with synthetic gene circuits act locally - Nature Biotechnology
Nature Biotechnology - Therapeutic T cells with synthetic gene circuits act locally
www.nature.com
February 5, 2025 at 7:59 PM
Research Highlight: Therapeutic T cells with synthetic gene circuits act locally www.nature.com/articles/s41...
Reposted by Jonathan KM Lim
Delighted to share our latest paper describing a method to read the levels of hundreds of metabolites or drugs in parallel using DNA sequencing. This method, which we call ‘smol-seq’ (Small MOLecule sequencing), harnesses the power of DNA sequencing for metabolite detection:
rdcu.be/d8xLv (1/6)
rdcu.be/d8xLv (1/6)
Quantifying metabolites using structure-switching aptamers coupled to DNA sequencing
Nature Biotechnology - Metabolites can be quantified using a combination of aptamers and DNA barcodes.
rdcu.be
February 4, 2025 at 1:54 PM
Delighted to share our latest paper describing a method to read the levels of hundreds of metabolites or drugs in parallel using DNA sequencing. This method, which we call ‘smol-seq’ (Small MOLecule sequencing), harnesses the power of DNA sequencing for metabolite detection:
rdcu.be/d8xLv (1/6)
rdcu.be/d8xLv (1/6)
Reposted by Jonathan KM Lim
Wow, that's a fantastic preprint from Neil Carragher's group @uoe-igc.bsky.social @margaretframe.bsky.social (couldn't find the others on bluesky yet):
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Drug combinations targeting FAK and MEK overcomes tumour heterogeneity in glioblastoma
Glioblastoma (GBM) is an aggressive brain tumour with limited treatment options and poor prognosis, largely due to its heterogeneity and the involvement of multiple intracellular signalling pathways t...
www.biorxiv.org
January 31, 2025 at 7:28 PM
Wow, that's a fantastic preprint from Neil Carragher's group @uoe-igc.bsky.social @margaretframe.bsky.social (couldn't find the others on bluesky yet):
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Jonathan KM Lim
New preprint from the lab! 🚨
🧫 New galactose #CRISPR screening strategy
🎯 481 genes required for #OXPHOS
⚙️ #FAM136A in IMS proteostasis
👂Mito defects in #Menieres disease
by @marcellharhai.bsky.social @fbm-unil.bsky.social
A short thread 1/5
www.biorxiv.org/content/10.1...
🧫 New galactose #CRISPR screening strategy
🎯 481 genes required for #OXPHOS
⚙️ #FAM136A in IMS proteostasis
👂Mito defects in #Menieres disease
by @marcellharhai.bsky.social @fbm-unil.bsky.social
A short thread 1/5
www.biorxiv.org/content/10.1...
January 30, 2025 at 7:58 AM
New preprint from the lab! 🚨
🧫 New galactose #CRISPR screening strategy
🎯 481 genes required for #OXPHOS
⚙️ #FAM136A in IMS proteostasis
👂Mito defects in #Menieres disease
by @marcellharhai.bsky.social @fbm-unil.bsky.social
A short thread 1/5
www.biorxiv.org/content/10.1...
🧫 New galactose #CRISPR screening strategy
🎯 481 genes required for #OXPHOS
⚙️ #FAM136A in IMS proteostasis
👂Mito defects in #Menieres disease
by @marcellharhai.bsky.social @fbm-unil.bsky.social
A short thread 1/5
www.biorxiv.org/content/10.1...
Reposted by Jonathan KM Lim
And this paper is coming to you live from #maternityleave with the right supportive network! Just wanted to encourage #womeninscience #youngparents
January 28, 2025 at 10:03 AM
And this paper is coming to you live from #maternityleave with the right supportive network! Just wanted to encourage #womeninscience #youngparents