Debraj Ghose
@debrajghose.bsky.social
I study how lymphocytes self-organize into multicellular structures. Also interested in how cells sense and respond to chemical gradients. Currently: Wyss Institute at Harvard. Previously: Comp Bio at Duke. debrajghose.com
Pinned
Movie S2 20230517
YouTube video by Debraj Shubham Ghose
youtu.be
Immune tissue behaves as shapeshifting active living matter as it fights intruders. Using liquid-like multicellular B cell droplets, we show that cytokines control the physical state of immune tissue.
www.biorxiv.org/content/10.1...
youtu.be/QAk2ayoVF0M
#biophysics #immunology #activematter
www.biorxiv.org/content/10.1...
youtu.be/QAk2ayoVF0M
#biophysics #immunology #activematter
Reposted by Debraj Ghose
Thrilled to share that a big piece of my thesis here in the Starr–Luxton is now out in Science Advances! We discovered that the cytoplasm of living animal tissues is extremely crowded and constrained, maintained by ribosomes and the giant KASH protein ANC-1.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Giant KASH proteins and ribosomes establish distinct cytoplasmic biophysical properties in vivo
Living tissues maintain unique intracellular biophysical properties under the control of cytoplasmic constraints and crowding.
www.science.org
September 10, 2025 at 7:10 PM
Thrilled to share that a big piece of my thesis here in the Starr–Luxton is now out in Science Advances! We discovered that the cytoplasm of living animal tissues is extremely crowded and constrained, maintained by ribosomes and the giant KASH protein ANC-1.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
1/5: New preprint: Cytokines control the physical state of immune tissue. www.biorxiv.org/content/10.1...
Unlike structured organs (heart, liver), immune tissue can shapeshift to form functional structures like germinal centers (GCs), tertiary lymphoid organs, granulomas, etc.
Unlike structured organs (heart, liver), immune tissue can shapeshift to form functional structures like germinal centers (GCs), tertiary lymphoid organs, granulomas, etc.
Cytokines control the physical state of immune tissue
Unlike most solid-like tissues, immune tissue is protean and reconfigurable—its component cells can patrol vast territories, find rare cellular partners, and dynamically self-organize into functional ...
www.biorxiv.org
November 18, 2025 at 9:31 PM
1/5: New preprint: Cytokines control the physical state of immune tissue. www.biorxiv.org/content/10.1...
Unlike structured organs (heart, liver), immune tissue can shapeshift to form functional structures like germinal centers (GCs), tertiary lymphoid organs, granulomas, etc.
Unlike structured organs (heart, liver), immune tissue can shapeshift to form functional structures like germinal centers (GCs), tertiary lymphoid organs, granulomas, etc.
Reposted by Debraj Ghose
How can cells self-organize rapidly into complex patterns during development?
Let’s explore a powerful and underappreciated mechanism: Directed Cell Migration (DCM).
Preprint @biorxivpreprint.bsky.social : doi.org/10.1101/2025...
👇Thread 🧵(1/11)
Let’s explore a powerful and underappreciated mechanism: Directed Cell Migration (DCM).
Preprint @biorxivpreprint.bsky.social : doi.org/10.1101/2025...
👇Thread 🧵(1/11)
July 30, 2025 at 7:00 AM
How can cells self-organize rapidly into complex patterns during development?
Let’s explore a powerful and underappreciated mechanism: Directed Cell Migration (DCM).
Preprint @biorxivpreprint.bsky.social : doi.org/10.1101/2025...
👇Thread 🧵(1/11)
Let’s explore a powerful and underappreciated mechanism: Directed Cell Migration (DCM).
Preprint @biorxivpreprint.bsky.social : doi.org/10.1101/2025...
👇Thread 🧵(1/11)
Reposted by Debraj Ghose
Such an honor to be featured by @focalplane.bsky.social , especially on my favorite day of the week: #FluorescenceFriday! 🔬
Below for reference, a picture of the full hydei testis with the musculature stained (depth-coded) taken together with @saraheclark.bsky.social
#cellbio #devbio #microscopy
Below for reference, a picture of the full hydei testis with the musculature stained (depth-coded) taken together with @saraheclark.bsky.social
#cellbio #devbio #microscopy
July 18, 2025 at 1:33 PM
Such an honor to be featured by @focalplane.bsky.social , especially on my favorite day of the week: #FluorescenceFriday! 🔬
Below for reference, a picture of the full hydei testis with the musculature stained (depth-coded) taken together with @saraheclark.bsky.social
#cellbio #devbio #microscopy
Below for reference, a picture of the full hydei testis with the musculature stained (depth-coded) taken together with @saraheclark.bsky.social
#cellbio #devbio #microscopy
Reposted by Debraj Ghose
Happy #FluorescenceFriday !!! Pt 2: fungal hyphae challenged with various oxidative stressors; nucleic acids - yellow, ROS - magenta feat. bubbles induced by H2O2 from earlier imaging experiments when I had no idea what I was doing :)
July 19, 2025 at 12:11 AM
Happy #FluorescenceFriday !!! Pt 2: fungal hyphae challenged with various oxidative stressors; nucleic acids - yellow, ROS - magenta feat. bubbles induced by H2O2 from earlier imaging experiments when I had no idea what I was doing :)
Reposted by Debraj Ghose
Stimulating immune cells in vitro is a common experimental lab model. We profiled 150K blood immune cells treated with 11 different stimuli to compare the effects. The data are freely available for researchers. See the preprint for our findings and to access the data 🧪 doi.org/10.1101/2025...
July 3, 2025 at 3:39 PM
Stimulating immune cells in vitro is a common experimental lab model. We profiled 150K blood immune cells treated with 11 different stimuli to compare the effects. The data are freely available for researchers. See the preprint for our findings and to access the data 🧪 doi.org/10.1101/2025...
Reposted by Debraj Ghose
FINALLY out in #Science Advances! 🎉
3 years of hard work, a fantastic team — and I’m proud to be first and co-corresponding author!
It's about Plexin signaling driving collective cell migration & organ sculpting. Check out the videos 😀
www.science.org/doi/10.1126/...
#DevBio #CellBio #Microscopy
3 years of hard work, a fantastic team — and I’m proud to be first and co-corresponding author!
It's about Plexin signaling driving collective cell migration & organ sculpting. Check out the videos 😀
www.science.org/doi/10.1126/...
#DevBio #CellBio #Microscopy
June 18, 2025 at 7:06 PM
FINALLY out in #Science Advances! 🎉
3 years of hard work, a fantastic team — and I’m proud to be first and co-corresponding author!
It's about Plexin signaling driving collective cell migration & organ sculpting. Check out the videos 😀
www.science.org/doi/10.1126/...
#DevBio #CellBio #Microscopy
3 years of hard work, a fantastic team — and I’m proud to be first and co-corresponding author!
It's about Plexin signaling driving collective cell migration & organ sculpting. Check out the videos 😀
www.science.org/doi/10.1126/...
#DevBio #CellBio #Microscopy
Reposted by Debraj Ghose
HIGH-RESOLUTION IN VIVO BRAIN IMAGING OF DROSOPHILA 🪰
New Preprint by Tassara et al.!
www.biorxiv.org/content/10.1...
#FluorescenceMonday #SnoutClub 1/6
New Preprint by Tassara et al.!
www.biorxiv.org/content/10.1...
#FluorescenceMonday #SnoutClub 1/6
February 26, 2025 at 2:48 AM
HIGH-RESOLUTION IN VIVO BRAIN IMAGING OF DROSOPHILA 🪰
New Preprint by Tassara et al.!
www.biorxiv.org/content/10.1...
#FluorescenceMonday #SnoutClub 1/6
New Preprint by Tassara et al.!
www.biorxiv.org/content/10.1...
#FluorescenceMonday #SnoutClub 1/6
Reposted by Debraj Ghose
A perfectly round liver organoid stained for mitochondria (yellowish/green), e-cadherin in blue and nuclei in pink for #MicroscopyMonday
June 2, 2025 at 6:31 PM
A perfectly round liver organoid stained for mitochondria (yellowish/green), e-cadherin in blue and nuclei in pink for #MicroscopyMonday
Reposted by Debraj Ghose
Excited to share our latest paper in PLOS Comp Bio, describing how accurate, multi-species segmentation models for bacteria can be created quickly and easily using image-to-image translation. 🧪⚛️
#biophysics #imageanalysis #microscopy #AI
dx.doi.org/10.1371/jour...
#biophysics #imageanalysis #microscopy #AI
dx.doi.org/10.1371/jour...
Segmentation of dense and multi-species bacterial colonies using models trained on synthetic microscopy images
Author summary Bacteria organize themselves on surfaces in ways that influence the spread of infections, but studying these behaviors is difficult, especially when multiple species are involved or the...
dx.doi.org
April 11, 2025 at 7:51 PM
Excited to share our latest paper in PLOS Comp Bio, describing how accurate, multi-species segmentation models for bacteria can be created quickly and easily using image-to-image translation. 🧪⚛️
#biophysics #imageanalysis #microscopy #AI
dx.doi.org/10.1371/jour...
#biophysics #imageanalysis #microscopy #AI
dx.doi.org/10.1371/jour...
Reposted by Debraj Ghose
Bonus: ran a quick color variation test — because it's always worth playing with presentation.
Color isn’t just style — it shapes what you see.
#MicroscopyCommunity #ScientificVisualization #Biophysics
Color isn’t just style — it shapes what you see.
#MicroscopyCommunity #ScientificVisualization #Biophysics
April 14, 2025 at 7:01 PM
Bonus: ran a quick color variation test — because it's always worth playing with presentation.
Color isn’t just style — it shapes what you see.
#MicroscopyCommunity #ScientificVisualization #Biophysics
Color isn’t just style — it shapes what you see.
#MicroscopyCommunity #ScientificVisualization #Biophysics
Reposted by Debraj Ghose
#Microtubule-driven cell shape changes and #actomyosin flow synergize to position the #centrosome, say Alexandre Schaeffer, Manuel Théry (@manuelthery.bsky.social ) and colleagues: rupress.org/jcb/article/...
@lblanchoin.bsky.social
@gelinmatthieu.bsky.social
#Biophysics #Cytoskeleton
@lblanchoin.bsky.social
@gelinmatthieu.bsky.social
#Biophysics #Cytoskeleton
April 24, 2025 at 2:56 PM
#Microtubule-driven cell shape changes and #actomyosin flow synergize to position the #centrosome, say Alexandre Schaeffer, Manuel Théry (@manuelthery.bsky.social ) and colleagues: rupress.org/jcb/article/...
@lblanchoin.bsky.social
@gelinmatthieu.bsky.social
#Biophysics #Cytoskeleton
@lblanchoin.bsky.social
@gelinmatthieu.bsky.social
#Biophysics #Cytoskeleton
Reposted by Debraj Ghose
My #FlyWeek contribution:
The Variety of Testis Shapes in Pomace Fly Species
Aren't they beatiful?🌈🪰🔬
An old-school sci-poster meets modern confocal imaging!
#Drosophila testis diversity fascinated even Curt Stern (yes, the human genetics textbook guy!) back in the 1930s.
#microscopy #insects
The Variety of Testis Shapes in Pomace Fly Species
Aren't they beatiful?🌈🪰🔬
An old-school sci-poster meets modern confocal imaging!
#Drosophila testis diversity fascinated even Curt Stern (yes, the human genetics textbook guy!) back in the 1930s.
#microscopy #insects
May 8, 2025 at 2:06 PM
My #FlyWeek contribution:
The Variety of Testis Shapes in Pomace Fly Species
Aren't they beatiful?🌈🪰🔬
An old-school sci-poster meets modern confocal imaging!
#Drosophila testis diversity fascinated even Curt Stern (yes, the human genetics textbook guy!) back in the 1930s.
#microscopy #insects
The Variety of Testis Shapes in Pomace Fly Species
Aren't they beatiful?🌈🪰🔬
An old-school sci-poster meets modern confocal imaging!
#Drosophila testis diversity fascinated even Curt Stern (yes, the human genetics textbook guy!) back in the 1930s.
#microscopy #insects
Reposted by Debraj Ghose
indeed, i begin my grad lecture on RNAi/miRNAs by discussing petunias. and mentioning while none of our students will do a plant phd (no faculty), they can learn A LOT by occasionally reading plant literature (think epigenetics, small RNAs, developmental biology, etc etc). also, consider the worm!
The quest to make darker petunias led to a 2006 Nobel & a fundamental tool of modern molecular biology.
In 1986, Richard Jorgensen was trying to make a darker purple petunia to get some VC funding. So he inserted additional copies of the pigment gene into petunias.
Instead, they turned WHITE.
In 1986, Richard Jorgensen was trying to make a darker purple petunia to get some VC funding. So he inserted additional copies of the pigment gene into petunias.
Instead, they turned WHITE.
May 8, 2025 at 12:31 AM
indeed, i begin my grad lecture on RNAi/miRNAs by discussing petunias. and mentioning while none of our students will do a plant phd (no faculty), they can learn A LOT by occasionally reading plant literature (think epigenetics, small RNAs, developmental biology, etc etc). also, consider the worm!
Reposted by Debraj Ghose
What governs where DNA replication begins in human cells? Here, we show that origins of replication in human DNA are epigenetically specified and that crucially, this epigenetic mark is required for both DNA replication and cell proliferation! @narjournal.bsky.social academic.oup.com/nar/article/...
May 6, 2025 at 9:39 PM
What governs where DNA replication begins in human cells? Here, we show that origins of replication in human DNA are epigenetically specified and that crucially, this epigenetic mark is required for both DNA replication and cell proliferation! @narjournal.bsky.social academic.oup.com/nar/article/...
Reposted by Debraj Ghose
If you're curious about how cells make decisions in complex environments, and how mathematical models can capture such behaviour, our preprint is now live!
Check it out here: www.biorxiv.org/content/10.1...
Check it out here: www.biorxiv.org/content/10.1...
Investigating Local Negative Feedback of Rac Activity by Mathematical Models and Cell Motility Simulations
For polarization and directed migration, cells use a combination of local positive feedback and long-range inhibition. We have previously used mathematical models to show the ability of this core…
www.biorxiv.org
May 6, 2025 at 6:09 PM
If you're curious about how cells make decisions in complex environments, and how mathematical models can capture such behaviour, our preprint is now live!
Check it out here: www.biorxiv.org/content/10.1...
Check it out here: www.biorxiv.org/content/10.1...
We found that cells use emergent collective memory—arising from simple chemical reactions—to outperform physical limits of detecting chemical gradients. Curious how? 1/n
8 min talk: www.youtube.com/watch?v=A7XH...
bioRxiv: www.biorxiv.org/content/10.1...
#biophysics #cellbiology
8 min talk: www.youtube.com/watch?v=A7XH...
bioRxiv: www.biorxiv.org/content/10.1...
#biophysics #cellbiology
Local collective memory from ratiometric signaling outperforms cellular gradient sensing limits
YouTube video by Debraj Shubham Ghose
www.youtube.com
May 5, 2025 at 10:11 AM
We found that cells use emergent collective memory—arising from simple chemical reactions—to outperform physical limits of detecting chemical gradients. Curious how? 1/n
8 min talk: www.youtube.com/watch?v=A7XH...
bioRxiv: www.biorxiv.org/content/10.1...
#biophysics #cellbiology
8 min talk: www.youtube.com/watch?v=A7XH...
bioRxiv: www.biorxiv.org/content/10.1...
#biophysics #cellbiology
Reposted by Debraj Ghose
U2OS labeled with OMP25-GFP, an outer membrane marker for mitochondria.
Left shows a zoom-in on the dimmer cell.
Imaged by OPM (oblique plane microscope).
#Microscopy
Left shows a zoom-in on the dimmer cell.
Imaged by OPM (oblique plane microscope).
#Microscopy
December 11, 2024 at 5:55 PM
U2OS labeled with OMP25-GFP, an outer membrane marker for mitochondria.
Left shows a zoom-in on the dimmer cell.
Imaged by OPM (oblique plane microscope).
#Microscopy
Left shows a zoom-in on the dimmer cell.
Imaged by OPM (oblique plane microscope).
#Microscopy
Reposted by Debraj Ghose
For #FluorescenceFriday, tau (blue) and long loops of neurofilament proteins are shown in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy
January 17, 2025 at 9:22 PM
For #FluorescenceFriday, tau (blue) and long loops of neurofilament proteins are shown in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy
Reposted by Debraj Ghose
It's #FluorescenceFriday! Here goes one image showing pathological Tau🟢 spreading in the monkey hippocampus. Neurons in 🟢 are dying, and healthy neurons in 🟣. Pathology spreads in a prion-like manner in this novel monkey model of Alzheimer we developed. How do we know that?
January 18, 2025 at 12:26 AM
It's #FluorescenceFriday! Here goes one image showing pathological Tau🟢 spreading in the monkey hippocampus. Neurons in 🟢 are dying, and healthy neurons in 🟣. Pathology spreads in a prion-like manner in this novel monkey model of Alzheimer we developed. How do we know that?
Reposted by Debraj Ghose
Could inhaling Xenon be used someday to prevent or treat Alzheimer's disease? An intriguing experimental model study shows its effect on modulating brain microglia and reducing inflammation
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Inhaled xenon modulates microglia and ameliorates disease in mouse models of amyloidosis and tauopathy
Xenon inhalation modulates microglial responses in mice and is a potential therapeutic agent for treating Alzheimer’s disease.
www.science.org
January 15, 2025 at 7:37 PM
Could inhaling Xenon be used someday to prevent or treat Alzheimer's disease? An intriguing experimental model study shows its effect on modulating brain microglia and reducing inflammation
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Debraj Ghose
Cool story on an IL-33-ILC2-myeloid cell circuit that promotes tertiary lymphoid structure development in tumors #ImmunoSky 🧪⚕️
"our findings highlight IL-33 as a candidate inflammatory TLS initiator, and ILC2s as a candidate inducer, of such a pathway."
"our findings highlight IL-33 as a candidate inflammatory TLS initiator, and ILC2s as a candidate inducer, of such a pathway."
IL-33-activated ILC2s induce tertiary lymphoid structures in pancreatic cancer - Nature
IL-33 induces tertiary lymphoid structures.
www.nature.com
January 18, 2025 at 7:03 PM
Cool story on an IL-33-ILC2-myeloid cell circuit that promotes tertiary lymphoid structure development in tumors #ImmunoSky 🧪⚕️
"our findings highlight IL-33 as a candidate inflammatory TLS initiator, and ILC2s as a candidate inducer, of such a pathway."
"our findings highlight IL-33 as a candidate inflammatory TLS initiator, and ILC2s as a candidate inducer, of such a pathway."