Rachael Ott
@rachaelott.bsky.social
Neurobiologist @UofSC 🔬 Super-resolution #microscopy, synaptic plasticity, cytoskeleton, #neurodegeneration 🦠
Pinned
Rachael Ott
@rachaelott.bsky.social
· Jan 17
For #FluorescenceFriday, tau (blue) and long loops of neurofilament proteins are shown in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy
Reposted by Rachael Ott
Hydra (Hydra vulgaris) ✨Small but mighty! Hydra can regenerate its entire body, even its head 🧠 A classic model for regeneration, stem cell dynamics, and body axis patterning 📸 Image by Daniel Bressan de Andrade #ModelMonday #DevBio #Regeneration
November 11, 2025 at 2:28 AM
Hydra (Hydra vulgaris) ✨Small but mighty! Hydra can regenerate its entire body, even its head 🧠 A classic model for regeneration, stem cell dynamics, and body axis patterning 📸 Image by Daniel Bressan de Andrade #ModelMonday #DevBio #Regeneration
Reposted by Rachael Ott
Happy #MicroscopyMonday everyone! 🌟🔬 Here’s an image from Ivy of outer hair cells (OHCs) and inner hair cells (IHCs) from a P20 mouse.. 🐁✨#Microscopy #HearingResearch #Cochlea #Neuroscience #Neuroskyence
November 10, 2025 at 8:01 AM
Happy #MicroscopyMonday everyone! 🌟🔬 Here’s an image from Ivy of outer hair cells (OHCs) and inner hair cells (IHCs) from a P20 mouse.. 🐁✨#Microscopy #HearingResearch #Cochlea #Neuroscience #Neuroskyence
Reposted by Rachael Ott
Check out this sneaky lamin A knockdown cell (pink dot) swimming against the tide (migration into gap) & slithering between its sisters.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.
November 7, 2025 at 4:29 PM
Check out this sneaky lamin A knockdown cell (pink dot) swimming against the tide (migration into gap) & slithering between its sisters.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.
It also slides over another nucleus that deforms underneath it.
MCF10A cells, #Spirochrome SPY650-DNA, 20 min time-lapse, taken on a #Zeiss #CellDiscoverer7.
Reposted by Rachael Ott
my first #FluorescenceFriday post! Mouse hippocampus showing microglia, a lysosomal marker (CD68), and Aggrecan, a perineuronal net component. 😍 Image by Dr. Julia Dziabis!
November 7, 2025 at 6:55 PM
my first #FluorescenceFriday post! Mouse hippocampus showing microglia, a lysosomal marker (CD68), and Aggrecan, a perineuronal net component. 😍 Image by Dr. Julia Dziabis!
Reposted by Rachael Ott
Happy #FluorescenceFriday from these primary astrocytes grown on glass slides and robotically stained on the Biocare Oncore Pro X! 🧠🔬🧪
Cyan = GFAP
Royal Purple = ALDH1L1
Salmon = Nuclear Counterstain
Cyan = GFAP
Royal Purple = ALDH1L1
Salmon = Nuclear Counterstain
November 7, 2025 at 7:26 PM
Happy #FluorescenceFriday from these primary astrocytes grown on glass slides and robotically stained on the Biocare Oncore Pro X! 🧠🔬🧪
Cyan = GFAP
Royal Purple = ALDH1L1
Salmon = Nuclear Counterstain
Cyan = GFAP
Royal Purple = ALDH1L1
Salmon = Nuclear Counterstain
Reposted by Rachael Ott
For #FluorescenceFriday - one of my favorites from the archives of a neuron ⚪ and astrocyte 🟢 co-culture. Image credit to Stephanie Page and former research assistant Beth Pattie 🧠🔬🧪
November 7, 2025 at 12:37 PM
For #FluorescenceFriday - one of my favorites from the archives of a neuron ⚪ and astrocyte 🟢 co-culture. Image credit to Stephanie Page and former research assistant Beth Pattie 🧠🔬🧪
Reposted by Rachael Ott
Flashback #FluorescenceFriday to some of the first light-sheet imaging we were doing. Video shows the collecting duct system of the developing kidney labeled with a pan-cytokeratin antibody. Courtesy of former talented technician Deanna Hardesty.
November 7, 2025 at 4:43 PM
Flashback #FluorescenceFriday to some of the first light-sheet imaging we were doing. Video shows the collecting duct system of the developing kidney labeled with a pan-cytokeratin antibody. Courtesy of former talented technician Deanna Hardesty.
Reposted by Rachael Ott
An iPSC heart muscle cell forming sarcomeres videoed through a microscope by Emma Koory for 20 h before it dies most dramatically. This was our first try on a new microscope, and Emma now has the imaging parameters dialed in so her cells do not go POP! Alpha-actinin-2 is shown. #CellBiology
October 31, 2025 at 2:46 PM
An iPSC heart muscle cell forming sarcomeres videoed through a microscope by Emma Koory for 20 h before it dies most dramatically. This was our first try on a new microscope, and Emma now has the imaging parameters dialed in so her cells do not go POP! Alpha-actinin-2 is shown. #CellBiology
Reposted by Rachael Ott
#FluorescenceFriday
@arvoinfo.bsky.social scientific image contest
(voting link below)
👇👁️👇: microglia imaged in the retina, color-coded for depth
ARVO Image Contest
www.arvo.org/news/scienti...
@flaumeye.bsky.social @cvsuor.bsky.social @urochestersmd.bsky.social @urneuroscience.bsky.social
@arvoinfo.bsky.social scientific image contest
(voting link below)
👇👁️👇: microglia imaged in the retina, color-coded for depth
ARVO Image Contest
www.arvo.org/news/scienti...
@flaumeye.bsky.social @cvsuor.bsky.social @urochestersmd.bsky.social @urneuroscience.bsky.social
November 7, 2025 at 5:11 AM
#FluorescenceFriday
@arvoinfo.bsky.social scientific image contest
(voting link below)
👇👁️👇: microglia imaged in the retina, color-coded for depth
ARVO Image Contest
www.arvo.org/news/scienti...
@flaumeye.bsky.social @cvsuor.bsky.social @urochestersmd.bsky.social @urneuroscience.bsky.social
@arvoinfo.bsky.social scientific image contest
(voting link below)
👇👁️👇: microglia imaged in the retina, color-coded for depth
ARVO Image Contest
www.arvo.org/news/scienti...
@flaumeye.bsky.social @cvsuor.bsky.social @urochestersmd.bsky.social @urneuroscience.bsky.social
Reposted by Rachael Ott
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
November 4, 2025 at 10:40 PM
My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...
Reposted by Rachael Ott
Rise of the Arteries — a lightsheet image capturing the intricate vascular network of a developing mouse heart.
By visualising endothelial and smooth muscle cells in 3D, researchers at IDRM reveal the beauty of coronary vessel formation.
📷 Credit: Konstantinos Miti & Polen Bareke
By visualising endothelial and smooth muscle cells in 3D, researchers at IDRM reveal the beauty of coronary vessel formation.
📷 Credit: Konstantinos Miti & Polen Bareke
October 30, 2025 at 3:28 PM
Rise of the Arteries — a lightsheet image capturing the intricate vascular network of a developing mouse heart.
By visualising endothelial and smooth muscle cells in 3D, researchers at IDRM reveal the beauty of coronary vessel formation.
📷 Credit: Konstantinos Miti & Polen Bareke
By visualising endothelial and smooth muscle cells in 3D, researchers at IDRM reveal the beauty of coronary vessel formation.
📷 Credit: Konstantinos Miti & Polen Bareke
Reposted by Rachael Ott
Can't believe it — my first‑author paper is out and my image graces the cover of @dev-journal.bsky.social 🎉
Here, we reveal how early developmental programs shape and maintain #zebrafish gill architecture throughout life
🔗 journals.biologists.com/dev/issue/15...
#FluorescenceFriday #LifelongDevSI
Here, we reveal how early developmental programs shape and maintain #zebrafish gill architecture throughout life
🔗 journals.biologists.com/dev/issue/15...
#FluorescenceFriday #LifelongDevSI
October 31, 2025 at 10:55 AM
Can't believe it — my first‑author paper is out and my image graces the cover of @dev-journal.bsky.social 🎉
Here, we reveal how early developmental programs shape and maintain #zebrafish gill architecture throughout life
🔗 journals.biologists.com/dev/issue/15...
#FluorescenceFriday #LifelongDevSI
Here, we reveal how early developmental programs shape and maintain #zebrafish gill architecture throughout life
🔗 journals.biologists.com/dev/issue/15...
#FluorescenceFriday #LifelongDevSI
Reposted by Rachael Ott
Endocardium, cells lining the heart chambers, in the beating heart of a two-day-old transgenic #zebrafish. Credit to @anjaliesch.bsky.social & Nikon Small World Competition. #ZebrafishZunday 🧪
October 26, 2025 at 8:06 AM
Endocardium, cells lining the heart chambers, in the beating heart of a two-day-old transgenic #zebrafish. Credit to @anjaliesch.bsky.social & Nikon Small World Competition. #ZebrafishZunday 🧪
Reposted by Rachael Ott
Alzheimer’s disease features amyloid-beta (Abeta) plaques, ApoE-driven amyloid buildup, and Caveolin1-modulated APP processing. IBA1 marks activated microglia clustering near plaques, fueling immune and inflammatory responses that accelerate neurodegeneration.
#FluorescenceFriday
#Neuroscience
#FluorescenceFriday
#Neuroscience
October 24, 2025 at 4:05 PM
Alzheimer’s disease features amyloid-beta (Abeta) plaques, ApoE-driven amyloid buildup, and Caveolin1-modulated APP processing. IBA1 marks activated microglia clustering near plaques, fueling immune and inflammatory responses that accelerate neurodegeneration.
#FluorescenceFriday
#Neuroscience
#FluorescenceFriday
#Neuroscience
Reposted by Rachael Ott
Meet the real rainbow fish 🌈🐟 Using Cre-mediated recombination, each zebrafish cell expresses a unique fluorescent color, creating a living rainbow of developmental history 🎨✨ Image by Bret Pearson #FluorescenceFriday #Zebrafish #DevBio
October 24, 2025 at 7:11 PM
Meet the real rainbow fish 🌈🐟 Using Cre-mediated recombination, each zebrafish cell expresses a unique fluorescent color, creating a living rainbow of developmental history 🎨✨ Image by Bret Pearson #FluorescenceFriday #Zebrafish #DevBio
Reposted by Rachael Ott
As spooky season is upon us 🎃, we have cells that look like spider webs for #FluorescenceFriday 🧪🔬
October 24, 2025 at 7:03 PM
As spooky season is upon us 🎃, we have cells that look like spider webs for #FluorescenceFriday 🧪🔬
Reposted by Rachael Ott
#fluorescencefriday Side by side comparison of alpha synuclein S129 (magenta), a marker of #parkinson’s , in M83 hemyzigous mice injected with human pre formed fibrils @shahrzadbahrampour.bsky.social Vlad Novikov clearing with SHIELD @lifecanvastech.bsky.social imaged at MIND platform @westernu.ca
October 24, 2025 at 4:56 PM
#fluorescencefriday Side by side comparison of alpha synuclein S129 (magenta), a marker of #parkinson’s , in M83 hemyzigous mice injected with human pre formed fibrils @shahrzadbahrampour.bsky.social Vlad Novikov clearing with SHIELD @lifecanvastech.bsky.social imaged at MIND platform @westernu.ca
Reposted by Rachael Ott
Reposted by Rachael Ott
For #FluorescenceFriday - projection neurons from the mouse prefrontal cortex to the locus coeruleus 🔴 with Fos labeling activated cells 🟢. Image credit to the Circuits team in the Translational Neuroscience Division @lieberinstitute.bsky.social 🧠🔬🧪
October 24, 2025 at 11:45 AM
For #FluorescenceFriday - projection neurons from the mouse prefrontal cortex to the locus coeruleus 🔴 with Fos labeling activated cells 🟢. Image credit to the Circuits team in the Translational Neuroscience Division @lieberinstitute.bsky.social 🧠🔬🧪
Reposted by Rachael Ott
The #mesoSPIM community just celebrated 10 years of open-source light-sheet microscopy in Zürich! For #3DThursday, we're honoring Martina Schaettin & Fabian Voigt’s iconic 3D chick embryo dataset — a luminous benchmark for open science and shared discovery.
#Microscopy #Lightsheet #syGlass
#Microscopy #Lightsheet #syGlass
October 23, 2025 at 3:33 PM
The #mesoSPIM community just celebrated 10 years of open-source light-sheet microscopy in Zürich! For #3DThursday, we're honoring Martina Schaettin & Fabian Voigt’s iconic 3D chick embryo dataset — a luminous benchmark for open science and shared discovery.
#Microscopy #Lightsheet #syGlass
#Microscopy #Lightsheet #syGlass
Reposted by Rachael Ott
Testing some new antibodies to identify oligodendrocytes 🟣, for me, the most difficult one to target in brain microscopy! Here I was able to identify its nuclei (oligo2 marker), not the whole cell body like neurons 🟡or microglia 🔵. Still, you can see oligos are everywhere! #Neuroscience #Microscopy
October 22, 2025 at 4:46 AM
Testing some new antibodies to identify oligodendrocytes 🟣, for me, the most difficult one to target in brain microscopy! Here I was able to identify its nuclei (oligo2 marker), not the whole cell body like neurons 🟡or microglia 🔵. Still, you can see oligos are everywhere! #Neuroscience #Microscopy
Reposted by Rachael Ott
For this #FluorescenceFriday 🔬
a cryosection of a dissected zebrafish gill, stained with phalloidin (orange) and DAPI (blue-ish).
#Zebrafish #DevBio #Microscopy
a cryosection of a dissected zebrafish gill, stained with phalloidin (orange) and DAPI (blue-ish).
#Zebrafish #DevBio #Microscopy
October 17, 2025 at 3:15 PM
For this #FluorescenceFriday 🔬
a cryosection of a dissected zebrafish gill, stained with phalloidin (orange) and DAPI (blue-ish).
#Zebrafish #DevBio #Microscopy
a cryosection of a dissected zebrafish gill, stained with phalloidin (orange) and DAPI (blue-ish).
#Zebrafish #DevBio #Microscopy
Reposted by Rachael Ott
Have a nice #FluorescenceFriday!
This is pollen from pine tree, imaged from auto-fluorescence by confocal microscopy
the LUT is "KTZ bw DarkGold" 🔥
This is pollen from pine tree, imaged from auto-fluorescence by confocal microscopy
the LUT is "KTZ bw DarkGold" 🔥
October 17, 2025 at 4:29 PM
Have a nice #FluorescenceFriday!
This is pollen from pine tree, imaged from auto-fluorescence by confocal microscopy
the LUT is "KTZ bw DarkGold" 🔥
This is pollen from pine tree, imaged from auto-fluorescence by confocal microscopy
the LUT is "KTZ bw DarkGold" 🔥
Reposted by Rachael Ott
We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
October 14, 2025 at 1:50 PM
We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
Reposted by Rachael Ott
Perspective from Richard McIntosh describing the history of research on #microtubule polymerization in terms of the ideas, technologies, and observations that have emerged as countless researchers have studied the dynamics of these essential cytoskeletal polymers. rupress.org/jcb/article/...
October 15, 2025 at 4:02 PM
Perspective from Richard McIntosh describing the history of research on #microtubule polymerization in terms of the ideas, technologies, and observations that have emerged as countless researchers have studied the dynamics of these essential cytoskeletal polymers. rupress.org/jcb/article/...