Mitosis Lab
@mitosislab.bsky.social
Structural cell biology @ University of Virginia Molecular Physiology and Biological Physics
Former @ Dept. of Biological Sciences & CBIS, NUS
anaphase.org
med.virginia.edu/faculty/faculty-listing/wwh6xu
mstdn.science/@MitosisLab
Former @ Dept. of Biological Sciences & CBIS, NUS
anaphase.org
med.virginia.edu/faculty/faculty-listing/wwh6xu
mstdn.science/@MitosisLab
Pinned
Mitosis Lab
@mitosislab.bsky.social
· Mar 17
Happy that Jon Chen @jonchenjk.bsky.social's cryo-ET and confocal study of RPE-1 chromatin in situ is online www.embopress.org/doi/full/10.1038/s44318-025-00407-2
Congrats also to coauthors Tingsheng Liu, @shujuncai.bsky.social, Cai Tong Ng, Jian Shi and collaborators Weimei Ruan Uttam Surana.
Congrats also to coauthors Tingsheng Liu, @shujuncai.bsky.social, Cai Tong Ng, Jian Shi and collaborators Weimei Ruan Uttam Surana.
Reposted by Mitosis Lab
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Human RPA is an essential telomerase processivity factor for maintaining telomeres
Telomerase counteracts telomere shortening by repeatedly adding DNA repeats to chromosome ends. We identified the replication protein A (RPA) heterotrimer as a telomerase processivity factor critical ...
www.science.org
October 30, 2025 at 10:07 PM
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Reposted by Mitosis Lab
Happy to share our latest publication, in which we show that the arrangement of nucleosomes around CTCF sites contributes to higher-order organisation of chromatin into TADs: www.embopress.org/doi/full/10....
October 27, 2025 at 11:49 AM
Happy to share our latest publication, in which we show that the arrangement of nucleosomes around CTCF sites contributes to higher-order organisation of chromatin into TADs: www.embopress.org/doi/full/10....
Reposted by Mitosis Lab
Reposted by Mitosis Lab
This is huge: “A drug that provides near-perfect protection against H.I.V. with shots just twice a year will be made available at $40 per patient annually in low- and middle-income countries, offering new hope… making lenacapavir a realistic choice in countries with constrained resources.”
Philanthropies Strike a Promising Deal to Turn Back H.I.V.
www.nytimes.com
September 24, 2025 at 12:27 PM
This is huge: “A drug that provides near-perfect protection against H.I.V. with shots just twice a year will be made available at $40 per patient annually in low- and middle-income countries, offering new hope… making lenacapavir a realistic choice in countries with constrained resources.”
Reposted by Mitosis Lab
For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data.
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
September 19, 2025 at 1:36 PM
For something different, tried this approach on something on the other end of the spectrum - in situ ribosomes from low SNR 2DTM data.
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
Used the U2OS-grid 9 dataset from EMPIAR-12459 (699 movies; doi.org/10.1016/j.mo...).
Blob pick & 2D after denoising gave 12k particles. 2-class HR-HAIR --> 3.3 Å
Reposted by Mitosis Lab
Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET www.biorxiv.org/content/10.1101/2025.09.16.676641v1 #cryoem
September 19, 2025 at 9:16 AM
Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET www.biorxiv.org/content/10.1101/2025.09.16.676641v1 #cryoem
Reposted by Mitosis Lab
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
August 31, 2025 at 6:44 PM
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Reposted by Mitosis Lab
🏅 Congratulations to Sandra Schmid (@czbiohub) on receiving ASCB’s 2025 E.B. Wilson Medal! This award honors distinguished researchers for far-reaching contributions to cell biology over a lifetime in science—an achievement Sandra exemplifies. #ASCB #CellBiology
August 18, 2025 at 7:25 PM
🏅 Congratulations to Sandra Schmid (@czbiohub) on receiving ASCB’s 2025 E.B. Wilson Medal! This award honors distinguished researchers for far-reaching contributions to cell biology over a lifetime in science—an achievement Sandra exemplifies. #ASCB #CellBiology
Reposted by Mitosis Lab
Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants - Nature Communications
Many protein–protein interactions depend on Short Linear Motifs (SLiMs). In this study, the authors use large-scale binding assays, deep mutational scanning, and structural analysis to map SLiMs recog...
www.nature.com
August 15, 2025 at 9:26 PM
Just published: Our paper on high-throughput analysis of cyclin docking interactions, led by the group of Norman Davey @icr.ac.uk and with some small-complex-plus-peptide #cryoEM by @natalia-mg.bsky.social from my lab. Congratulations, everyone!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Mitosis Lab
My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!
Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!
Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!
Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
The small GTPase Ran defines nuclear pore complex asymmetry
Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…
www.sciencedirect.com
August 18, 2025 at 4:26 PM
My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!
Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!
Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!
Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
Reposted by Mitosis Lab
Our first expansion microscopy results. Imaged today on "normal" Zeiss LSM 900, revealing chromatin and RNA polymerase II clusters at an unreal level of detail.
Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏
Months of preparation are paying off now!
Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏
Months of preparation are paying off now!
August 14, 2025 at 6:28 PM
Our first expansion microscopy results. Imaged today on "normal" Zeiss LSM 900, revealing chromatin and RNA polymerase II clusters at an unreal level of detail.
Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏
Months of preparation are paying off now!
Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏
Months of preparation are paying off now!
Looks like us structural cell biologists will have to be satisfied with false-atomic resolution for awhile.
August 14, 2025 at 2:24 PM
Looks like us structural cell biologists will have to be satisfied with false-atomic resolution for awhile.
Reposted by Mitosis Lab
Check out 🧐 our new preprint 📜:
www.biorxiv.org/content/10.1...
Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠
#microsky #teamtomo #Chloroflexota
www.biorxiv.org/content/10.1...
Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠
#microsky #teamtomo #Chloroflexota
August 12, 2025 at 7:22 AM
Check out 🧐 our new preprint 📜:
www.biorxiv.org/content/10.1...
Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠
#microsky #teamtomo #Chloroflexota
www.biorxiv.org/content/10.1...
Congrats🎉 to @vasilgaisin.bsky.social and Corina Hadjicharalambous, who studied contractile injection systems directly in hot spring♨️ bacterial mats🦠
#microsky #teamtomo #Chloroflexota
Reposted by Mitosis Lab
Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...
August 1, 2025 at 3:27 PM
Check out our new preprint on the discovery of a molecular switch in NAC that mediates nascent chain sorting on the ribosome and prevents mitochondrial protein mistargeting by SRP. A great collaboration with the Shan Lab @Caltech and the Qi Lab @UVA: www.biorxiv.org/content/10.1...
Reposted by Mitosis Lab
Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out:
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV
Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report s…
www.sciencedirect.com
July 30, 2025 at 9:50 PM
Now out in Structure: Paper on high-resolution #cryoEM using the 100 kV Tundra cryo-TEM by Dimple Karia, Adrian Koh, @abhaykot.bsky.social, @lingboyu.bsky.social, @landerlab.bsky.social and many others. Happy to have contributed to this effort. Check it out:
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Mitosis Lab
New preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes?
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Global inhibition of deadenylation stabilizes the transcriptome in mitotic cells
In the presence of cell division errors, mammalian cells can pause in mitosis for tens of hours with little to no transcription, while still requiring continued translation for viability. These unique...
www.biorxiv.org
July 23, 2025 at 10:57 AM
New preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes?
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Mitosis Lab
July 21, 2025 at 7:15 PM
Reposted by Mitosis Lab
This seems like a really important paper comparing chromosome tracing methods, demonstrating that classical denaturing DNA-FISH and chromosome tracing leads to substantial distortion of 3D genome structure, likely due to the high-temperatures involved (70-90C):
www.nature.com/articles/s41...
www.nature.com/articles/s41...
July 20, 2025 at 7:39 PM
This seems like a really important paper comparing chromosome tracing methods, demonstrating that classical denaturing DNA-FISH and chromosome tracing leads to substantial distortion of 3D genome structure, likely due to the high-temperatures involved (70-90C):
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Mitosis Lab
Happy to share our latest work on the structure and assembly of holocentric kinetochores! Huge thanks to Ines for a very fruitful collaboration, Claudio for all the support, and congratulations to Christine and all co-authors!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
July 15, 2025 at 4:36 PM
Happy to share our latest work on the structure and assembly of holocentric kinetochores! Huge thanks to Ines for a very fruitful collaboration, Claudio for all the support, and congratulations to Christine and all co-authors!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Mitosis Lab
Frustrated with confusing or conflicting requests for revisions?
At eLife, editors and reviewers discuss their reviews with each other before reaching a decision on a manuscript. Learn more about peer review at eLife: buff.ly/ioUIZyz
At eLife, editors and reviewers discuss their reviews with each other before reaching a decision on a manuscript. Learn more about peer review at eLife: buff.ly/ioUIZyz
July 12, 2025 at 1:59 PM
Frustrated with confusing or conflicting requests for revisions?
At eLife, editors and reviewers discuss their reviews with each other before reaching a decision on a manuscript. Learn more about peer review at eLife: buff.ly/ioUIZyz
At eLife, editors and reviewers discuss their reviews with each other before reaching a decision on a manuscript. Learn more about peer review at eLife: buff.ly/ioUIZyz
Reposted by Mitosis Lab
Proud to share our work where we resolved a longstanding question regarding the existence of a human telomerase dimer and provided insights into its function. Led by 3 amazing lab members in collaboration with @yiliangding.bsky.social and @rdaslab.bsky.social.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerization
Telomerase ribonucleoprotein (RNP) synthesizes telomeric repeats at chromosome ends using a telomerase reverse transcriptase (TERT) and a telomerase RNA (hTR in humans). Previous structural work showe...
www.science.org
July 10, 2025 at 9:26 PM
Proud to share our work where we resolved a longstanding question regarding the existence of a human telomerase dimer and provided insights into its function. Led by 3 amazing lab members in collaboration with @yiliangding.bsky.social and @rdaslab.bsky.social.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Mitosis Lab
In our latest preprint on bioRxiv, we present the in situ structure of
the human gap junction at 14 Å resolution using cryo-ET.
The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.
the human gap junction at 14 Å resolution using cryo-ET.
The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.
July 8, 2025 at 5:33 AM
In our latest preprint on bioRxiv, we present the in situ structure of
the human gap junction at 14 Å resolution using cryo-ET.
The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.
the human gap junction at 14 Å resolution using cryo-ET.
The structure provides a blueprint for investigating how connexin
regulation shapes intercellular communication in health and disease.
Reposted by Mitosis Lab
Excited and honored to be joining the EMBO Member community 🥳 Congratulations also to all the other newly elected members! -Martin
Welcome and congratulations to the 60 new members and 9 associate members who have been elected to the EMBO Membership! This honour celebrates #research excellence and outstanding achievements in the #LifeSciences. 🧪
Learn more here:
www.embo.org/press-releas... #EMBOMembers2025
Learn more here:
www.embo.org/press-releas... #EMBOMembers2025
Outstanding life scientists elected to the EMBO Membership – Press releases – EMBO
Sixty-nine new members join the community of leading life scientists in Europe and beyond
www.embo.org
July 2, 2025 at 7:04 AM
Excited and honored to be joining the EMBO Member community 🥳 Congratulations also to all the other newly elected members! -Martin
Reposted by Mitosis Lab
Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that.
“Achieving individual tomograms of cells where any features are at 10 Å resolution or better is well-beyond both current capabilities and reasonable predictions of future improvements.”
:(
:(
Check out our recent review discussing the merits of tilt series vs single micrographs for in situ cryo-EM! www.sciencedirect.com/science/arti...
July 1, 2025 at 6:31 PM
Whoever gets the resolution of raw tomograms to <10 Å within the sampled parts of Fourier space would probably deserve a Nobel. Or they'd get one anyway for the crazy new physics they discovered to achieve that.
Reposted by Mitosis Lab
