Juanma García-Arcos
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juanmagararc.bsky.social
Juanma García-Arcos
@juanmagararc.bsky.social
610 followers 490 following 13 posts
Group leader at EPFL, Lausanne. Formerly, Postdoc Roux lab, Geneva; PhD Piel lab, Paris. #cytoplasts #actomyosin #cellbio #microscopy #membranes #biophysics #extracellular_vesicles #sciart
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juanmagararc.bsky.social
Juanma García-Arcos @juanmagararc.bsky.social · Oct 5
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.

www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
Reposted by Juanma García-Arcos
longpainter.bsky.social
Super moon. 8”x6” oil
November 9, 2025 at 4:48 PM
Reposted by Juanma García-Arcos
joachimgoedhart.bsky.social
Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects
www.biophysics-reports.org/article/doi/...
Figure 3. FLIM biosensors. Illustrative summary of environment‐sensing FLIM biosensors (excluding FLIM‐FRET). These biosensors monitor diverse cellular parameters in living cells, including: 1. cell cycle status (Tan et al. 2024); 2. molecular crowding (Levchenko et al. 2021); 3. membrane potential (van der Linden et al. 2021); 4. temperature (Okabe et al. 2012); 5. redox states (free versus protein‐bound NADH) (Sorrells et al. 2021); 6. protein activity (Mehl et al. 2024); 7. ion concentrations (e.g., Ca2+ (van der Linden et al. 2021) and H+ (Rennick et al. 2022))
November 3, 2025 at 11:24 AM
Reposted by Juanma García-Arcos
apdu-unige.bsky.social
Check the new preprint of @juanmagararc.bsky.social !
All you wanted to know about FLIPER and all the things you need to take in account before doing a FLIPER-TR experiment :)
October 15, 2025 at 1:24 PM
Reposted by Juanma García-Arcos
mhakala.bsky.social
If you are planning to do Flipper-TR experiments or FLIM imaging in general, I strongly recommend reading this beautiful method paper first! Lots of information about optimal data acquisition for reliable data analysis! Nice work from Tithi, @rouxlab.bsky.social and @juanmagararc.bsky.social !
October 22, 2025 at 8:54 AM
Reposted by Juanma García-Arcos
clairedessalles.bsky.social
All you've ever wanted to know about FLIM analysis (and its application in Flipper-TR)!😱🤩🔬
October 7, 2025 at 1:54 PM
Reposted by Juanma García-Arcos
spirochrome.com
An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.

Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
October 7, 2025 at 8:21 AM
Reposted by Juanma García-Arcos
joachimgoedhart.bsky.social
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM by Juan Manuel Garcia-Arcos and team: www.biorxiv.org/content/10.1...
Frequency dependence of fast-lifetime FLIM and fits for C2C12 cells.
September 26, 2025 at 1:29 PM
Reposted by Juanma García-Arcos
kojamf.bsky.social
Dr. Jane Goodall filmed an interview with Netflix in March 2025 that she understood would only be released after her death.
October 5, 2025 at 9:08 AM
juanmagararc.bsky.social
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.

www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
October 5, 2025 at 4:04 PM
Reposted by Juanma García-Arcos
spirochrome.com
📢New Actin Probe available at Spirochrome!

Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!

spirochrome.com/product/spy6...

The product is now available from our distributors

Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...
February 6, 2025 at 11:45 AM
Reposted by Juanma García-Arcos
pjsaez.bsky.social
How cells decide where to go? #CellMigration

Last year we published our 1st paper @cellcommlab.bsky.social, showing how actin-based membrane oscillations define a new direction ( #cancer & non-cancer #cells )

Full www.nature.com/articles/s41...
@naturephysics.bsky.social @focalplane.bsky.social 🧪🔬
January 18, 2025 at 11:24 PM
Reposted by Juanma García-Arcos
pjsaez.bsky.social
#CellMigration happens all the time in our bodies. This may (or not) rely on adhesion molecules

#Cancer cells switch strategies during metastasis to invade other tissues

Naomi Wee (naomiwee.com) did this neat movie to explain it:
www.youtube.com/watch?v=D9aD...
#SciSky 🧪🔬 @cellcommlab.bsky.social
February 6, 2025 at 10:44 AM
Reposted by Juanma García-Arcos
ricardalert.bsky.social
New preprint! When and how do active flows become turbulent? We found that, in unconfined active nematics, the transition to turbulence is discontinuous! With @the-chaotician.bsky.social @mpipks.bsky.social

arxiv.org/abs/2501.060...
January 15, 2025 at 5:22 PM
Reposted by Juanma García-Arcos
bryanwelm.bsky.social
I mostly see the protein sculptures I build in the lighting of my workshop. It’s fairly static and dull lighting. When they are close to being finished, I bring them into the house and enjoy watching how they change under different natural lighting throughout the day. They are so much more dynamic!
Light reflecting off a sculpture of different structural domains found in proteins. Made from steel.
November 23, 2024 at 1:45 PM
Reposted by Juanma García-Arcos
itaiyanai.bsky.social
Would you prefer to lead a lab alone or together with a science buddy? How interesting would be a research institute where joint labs are the standard?!
November 22, 2024 at 11:42 PM
Reposted by Juanma García-Arcos
clausenlab.bsky.social
i might be biased, but this place is amazing: great colleagues, vibrant campus, brilliant students/postdocs - and Vienna! perfect for starting an ambitious project. apply now for a GL position at the IMP …

www.imp.ac.at/career/open-...
Group leader positions at the IMP | Research Institute of Molecular Pathology
www.imp.ac.at
November 19, 2024 at 5:30 PM
Reposted by Juanma García-Arcos
hellmichgroup.bsky.social
Ok, trying to bring my community back together... I created a starter pack for lipids and membrane protein aficionados!

Feel free to share, add and distribute, so we can come together again! 😍

go.bsky.app/AEeXh86
November 16, 2024 at 3:53 AM
Reposted by Juanma García-Arcos
guijacquemet.bsky.social
I started a list of people working broadly on cell adhesion and cell migration. I likely missed many, please drop me a message if you want to be added

go.bsky.app/5Gzo7oZ
November 11, 2024 at 1:09 PM
Reposted by Juanma García-Arcos
mads100tist.bsky.social
I have created a starter pack of scientists in der Schweiz so we can all hopefully find each other 🇨🇭🧪

go.bsky.app/8rF765Z
Scientists in Switzerland starter pack, with our avatars
November 19, 2024 at 8:46 PM
Reposted by Juanma García-Arcos
krishnamudumbi.bsky.social
Sunday #NewPI thoughts
Four panel Star Wars meme of Anakin and Padme in the grass. Anakin looking to his left at Padme. Starts off with Padme smiling but ends with her looking crestfallen
November 17, 2024 at 1:52 PM
Reposted by Juanma García-Arcos
aaandmoore.bsky.social
This is a movie of actin filaments in a cultured cell. I like watching it and I hope you do to.
November 18, 2024 at 5:50 AM
Reposted by Juanma García-Arcos
peiferlabunc.bsky.social
Cell and developmental biologists--Join us at the 2025 Cell Contact and Adhesion Gordon Conference. We've assembled a strong and diverse program of speakers at the cutting-edge of science in our field 1/n Please repost
www.grc.org/cell-contact...
Poster with images of cells and embryos, mountains and a list of speakers
November 11, 2024 at 3:55 PM
Reposted by Juanma García-Arcos
deniswirtz.bsky.social
Download our database of 492 (!) funding opportunities for EARLY-CAREER FACULTY AND RESEARCHERS.

For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship).

Download freely here: research.jhu.edu/rdt/funding-...
November 17, 2024 at 4:38 PM
Reposted by Juanma García-Arcos
pabrod.bsky.social
Adoro Mastodon, pero no he podido contenerme:

cc @irreductible.bsky.social
November 17, 2024 at 4:29 PM
Reposted by Juanma García-Arcos
danielsugarman.bsky.social
“I’ve noticed I’m not in your starter pack. Any particular reason for that?”
November 17, 2024 at 11:34 AM