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spirochrome.com
Spirochrome
@spirochrome.com
Our main focus is to offer innovative tools for live cell fluorescence imaging to the scientific community.
https://spirochrome.com/
👉🦠Interested in bacterial membrane tension?

Our membrane tension probe Flipper-TR is a top tool for the job!

A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.

🔗 doi.org/10.1073/pnas...
🧪
Exploring bacteria–surface interactions with a fluorescent membrane tension probe | PNAS
Understanding how bacteria interact with surfaces is critical for advancing applications in biofilm and biofouling prevention, biomaterial developm...
doi.org
November 4, 2025 at 9:19 AM
Reposted by Spirochrome
Find out how SPY650-FastAct_X can help your research here:

spirochrome.com/product/spy6...
October 29, 2025 at 9:30 AM
Reposted by Spirochrome
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!

1. Add the probe to the cells
2. Image 1h later

SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.

image credits: @stephanlab.bsky.social
October 29, 2025 at 9:30 AM
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!

1. Add the probe to the cells
2. Image 1h later

SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.

image credits: @stephanlab.bsky.social
October 29, 2025 at 9:30 AM
An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.

Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
October 7, 2025 at 8:21 AM
Reposted by Spirochrome
📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
October 2, 2025 at 8:40 AM
📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
October 2, 2025 at 8:40 AM
Reposted by Spirochrome
SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N
September 17, 2025 at 5:20 PM
Reposted by Spirochrome
Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪
September 29, 2025 at 12:46 PM
Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition!

His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.

www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
In vitro growth of chick sensory neurons | 2025 Small World in Motion Competition
Alexandre Dumoulin - In vitro growth of chick sensory neurons, shown in gold using a dye marking their internal structure (18 hour time lapse)
www.nikonsmallworld.com
September 26, 2025 at 8:59 AM
Reposted by Spirochrome
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.

Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...

Image credits: Olivier Mercey, UNIGE
September 19, 2025 at 9:45 AM
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.

Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...

Image credits: Olivier Mercey, UNIGE
September 19, 2025 at 9:45 AM
Amazing achievement!

Congratulations

Cherry on the cake, it's SPY555-Actin staining the actin cytoskeleton in the video 😍
SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N
September 18, 2025 at 7:02 PM
Reposted by Spirochrome
🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
Multispectral live-cell imaging with uncompromised spatiotemporal resolution - Nature Photonics
A tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with...
www.nature.com
September 16, 2025 at 10:20 AM
Reposted by Spirochrome
📄 🌟 Researchers introduce HAK-actin, an actin label compatible with post-labeling in #SuperResolution ultrastructure expansion #Microscopy #ExM. The authors demonstrate its use in cells expanded up to 16-fold (figure).

🔗 View the preprint article: bit.ly/47xC8NJ

#Microscope #Imaging #Fluorescence
September 10, 2025 at 1:41 PM
Reposted by Spirochrome
For all of you #Actin folks ... It's out !
Happy to help at anypoint
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...
September 10, 2025 at 9:11 AM
Reposted by Spirochrome
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...
September 10, 2025 at 7:58 AM
Reposted by Spirochrome
🧬 New tool for U-ExM!
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.

Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...
September 10, 2025 at 9:59 AM
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...
September 10, 2025 at 7:58 AM
A stunning application of SPY650-FastAct_X (actin)/SPY555-DNA (nuclei) staining iPSC cells and imaged by light sheet microscopy at UCSF. Thank you ‪@microtubule.bsky.social‬ for sharing it!
Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.
September 2, 2025 at 7:07 AM
Reposted by Spirochrome
A new #UExM probe for #Actin!

Fun times thinking/developing/testing with @centriolelab.bsky.social & @lreymond.bsky.social !

Congrats to all !

If interested in #UExM, or #HAK-Actin, feel free to reach out !

Soon available at @spirochrome.com

#Expansion #Microscopy #ProtistsOnSky
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
August 28, 2025 at 7:16 AM
Getting a bright & reliable actin staining in expansion microscopy was a tough nut until today!

The team gathered around @centriolelab.bsky.social found a hak:

HAK-actin, a new probe for U-ExM based protocols revealed in a new BioRxiv manuscript.

HAK-actin will soon be available at Spirochrome!
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
August 28, 2025 at 11:38 AM
Reposted by Spirochrome
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
August 28, 2025 at 7:03 AM
SPY650-FastAct_X (red) & SPY555-tubulin (blue) teaming up in this astrocyte to highlight mitochondria (white) and cytoskeleton interactions.

Thank you @aaandmoore.bsky.social‬ for sharing this amazing movie.
This is an airyscan confocal movie of mitochondria (white) moving around in a mouse astrocyte. Actin filaments are in orange and microtubules are in blue.
August 20, 2025 at 12:57 PM
Reposted by Spirochrome
Very happy to see our preprint on how the mechanosensitive ion channel Piezo1 controls intercellular junction maturation by balancing cortex and membrane tension now published in @jcellsci.bsky.social Congratulations to Ahsan & team! journals.biologists.com/jcs/article/...
August 7, 2025 at 6:13 AM