Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!

1. Add the probe to the cells
2. Image 1h later

SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.

image credits: @stephanlab.bsky.social

📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging

Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy

• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets

spirochrome.com/product/hak-...

Our Live cell actin probe SPY650-FastAct efficiently labels primary keratinocytes isolated from mice.

If you are interested in the context of this movie you can read the Science article by @loxstoplox.bsky.social about the role of actin in early embryo spindle formation and look at the other 😍 microscopy images and movies.

doi.org/10.1126/scie...

Let's start the week with this movie kindly shared by @loxstoplox.bsky.social
The movie shows a mouse embryo labeled with Formin-2-GFP and SPY650-DNA during nuclear envelope breakdown. Note the nuclear dilution of Formin-2-GFP immediately before the SPY650-DNA labeled chromosomes start compaction.

FastAct_X: the 3rd actin probes generation from Spirochrome

✅ Improved actin ligand, captures very fast actin dynamics
✅ No undesired F-actin stabilization
✅ Just add & image protocol
✅ High brightness
✅ STED compatible
✅ Available in two colors SPY555 & SPY650

spirochrome.com/products/?_r...

Highly impressive LLSM imaging of PKmito Orange stained cells using DeepCristae, a newly published mitochondrial cristae restoration convolutional neural network.

PKMito Orange: spirochrome.com/product/pkmi...

Source Article: doi.org/10.1038/s420...

Happy #FluorescenceFriday

📢New Actin Probe available at Spirochrome!

Introducing SPY650-FastAct_X our latest actin probe with highly improved kinetics and no toxicity!

spirochrome.com/product/spy6...

The product is now available from our distributors

Preprint from the @kjohnsson.bsky.social lab: doi.org/10.1101/2025...

A recent manuscript by @sulianamanley.bsky.social's lab led by @jclandoni.bsky.social describes reversible mitochondrial pearling as a driving force for mtDNA regular distribution within mitochondria.

PKmito ORANGE was used in to image mitochondrial pearling by STED microscopy in live cells.

Happy #MicroscopyMonday!

We have here a mouse embryo stained with PKmito Deep Red. It is happily undergoing two synchronized cell divisions rounds.
PKmito low phototoxicity is key for long term imaging of fragile embryos.

A warm welcome to all the new Bluesky members that recently joined!

Let's celebrate #FluorescenceFriday with @axonsalex.bsky.social's axonal growth sparks & fireworks!

More information in the movie alt-text

Our friends from Fluosphera, a Swiss high-throughput in-vitro drug discovery startup, uses spheroids encapsulated in alginate for multiplexing cell lines in a single well.
Our nuclear SPY-DNA probes work like a charm with their technology!