Alexandre Dumoulin
@axonsalex.bsky.social
Developmental Neurobiologist at UZH | Microscopy Enthusiast. Using the chicken embryo to decipher the molecular mechanisms of neural circuit formation. Fascinated by axonal growth cones, primary cilia, and extracellular vesicles.
Pinned
Zurich is ready! 🤘 looking forward to hosting you at our Fish and Chicks seminar!
On my way to Zurich to speak at the 'Fish n Chick' series at UZH, invited by the irreplaceable @axonsalex.bsky.social 🧪
November 5, 2025 at 7:12 AM
Zurich is ready! 🤘 looking forward to hosting you at our Fish and Chicks seminar!
Reposted by Alexandre Dumoulin
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
October 2, 2025 at 5:11 PM
Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky
Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪
Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition!
His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.
www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.
www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
In vitro growth of chick sensory neurons | 2025 Small World in Motion Competition
Alexandre Dumoulin - In vitro growth of chick sensory neurons, shown in gold using a dye marking their internal structure (18 hour time lapse)
www.nikonsmallworld.com
September 29, 2025 at 12:46 PM
Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪
Reposted by Alexandre Dumoulin
Check it out at PNAS today. Thank you Alexandre for the collaboration!!
PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!
Available @spirochrome.com
PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!
Available @spirochrome.com
Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social !
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
July 16, 2025 at 7:42 AM
Check it out at PNAS today. Thank you Alexandre for the collaboration!!
PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!
Available @spirochrome.com
PK Mem probes offer long time-lapse imaging of cell membrane dynamics. Made possible by
Jing Ling, Yitong Liu, and all the collaborators!
Available @spirochrome.com
Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social !
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
July 16, 2025 at 6:44 AM
Thrilled to collaborate with @zhixingchen2.bsky.social Lab @pku1898.bsky.social !
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
Reposted by Alexandre Dumoulin
Hot off the press:
The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience:
rdcu.be/ek010
The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience:
rdcu.be/ek010
Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster
Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...
rdcu.be
May 7, 2025 at 11:26 AM
Hot off the press:
The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience:
rdcu.be/ek010
The excellent Maheva Andriatsilavo and colleagues show how a temporal sequence of stochastic molecular mechanisms allow the emergence of stereotyped individualised neuronal circuits. Just published in Nature Neuroscience:
rdcu.be/ek010
Reposted by Alexandre Dumoulin
New from the lab: @maheandria.bsky.social and colleagues in collaboration with the Hiesinger, Stoeckli and von Kleist labs dissect the sequential emergence of axonal targeting during development in today's issue of Nature Neuroscience:
rdcu.be/ek010
rdcu.be/ek010
Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster
Nature Neuroscience - The developmental origin of behavioral individuality is unclear. The authors show that a temporal sequence of genetically encoded stochastic mechanisms explains variation in...
rdcu.be
May 7, 2025 at 11:31 AM
New from the lab: @maheandria.bsky.social and colleagues in collaboration with the Hiesinger, Stoeckli and von Kleist labs dissect the sequential emergence of axonal targeting during development in today's issue of Nature Neuroscience:
rdcu.be/ek010
rdcu.be/ek010
Reposted by Alexandre Dumoulin
These ones are growing in a dish, but in a developing embryo or brain, each growth cone would be sniffing its way through a complex molecular terrain, often over long distances to find its correct synaptic partners.
April 30, 2025 at 11:09 AM
These ones are growing in a dish, but in a developing embryo or brain, each growth cone would be sniffing its way through a complex molecular terrain, often over long distances to find its correct synaptic partners.
it is a filopodial (thin protusions) party in there! 🕺🧐
🧪 #liveimaging
🧪 #liveimaging
April 30, 2025 at 7:54 AM
it is a filopodial (thin protusions) party in there! 🕺🧐
🧪 #liveimaging
🧪 #liveimaging
This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging.
I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉
🧪
I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉
🧪
April 30, 2025 at 7:16 AM
This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging.
I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉
🧪
I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉
🧪
Reposted by Alexandre Dumoulin
Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!
January 20, 2025 at 10:22 AM
Please welcome our official #mesoSPIM channel on this platform, @mesospim.bsky.social. Repost is appreciated!
Reposted by Alexandre Dumoulin
A brief history of The Company of Biologists - 100 years of supporting scientists!👍👏
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
The Company of Biologists: celebrating 100 years
Summary: As we begin celebrating our 100-year anniversary, we reflect on our extraordinary story so far, consider the Company's current support for our communities and prepare for some inspiring activ...
journals.biologists.com
January 6, 2025 at 5:53 PM
A brief history of The Company of Biologists - 100 years of supporting scientists!👍👏
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
Reposted by Alexandre Dumoulin
Join us in beautiful Quintay to celebrate 25 years of the Practical Course on Developmental Biology!
To register go to biodesarrollo.unab.cl/mini-symposi...
Come to enjoy a unique blend of science, sea, and food – all for free!
To register go to biodesarrollo.unab.cl/mini-symposi...
Come to enjoy a unique blend of science, sea, and food – all for free!
January 4, 2025 at 11:13 AM
Join us in beautiful Quintay to celebrate 25 years of the Practical Course on Developmental Biology!
To register go to biodesarrollo.unab.cl/mini-symposi...
Come to enjoy a unique blend of science, sea, and food – all for free!
To register go to biodesarrollo.unab.cl/mini-symposi...
Come to enjoy a unique blend of science, sea, and food – all for free!
Reposted by Alexandre Dumoulin
Did you know 2024 was our 20th anniversary? We're closing it out by sharing a brief history of Addgene, with commentary from our incredible community.
Read it below!
blog.addgene.org/add...
Read it below!
blog.addgene.org/add...
Addgene: The First Twenty Years
Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.
blog.addgene.org
December 31, 2024 at 8:02 PM
Did you know 2024 was our 20th anniversary? We're closing it out by sharing a brief history of Addgene, with commentary from our incredible community.
Read it below!
blog.addgene.org/add...
Read it below!
blog.addgene.org/add...
Reposted by Alexandre Dumoulin
2024 #FluorescenceFriday retrospective: It was a great year in science for microglia, the brain's immune cells! The brain is much more than neurons; these little cells are essential, particularly in inflammatory processes. Check them interacting below! Markers: neurons (🔵 MAP2), microglia (🟡 IBA1)
December 27, 2024 at 7:35 PM
2024 #FluorescenceFriday retrospective: It was a great year in science for microglia, the brain's immune cells! The brain is much more than neurons; these little cells are essential, particularly in inflammatory processes. Check them interacting below! Markers: neurons (🔵 MAP2), microglia (🟡 IBA1)
Reposted by Alexandre Dumoulin
How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling.
www.biorxiv.org/content/10.1...
Special thanks to @aliceyting.bsky.social for discussions
www.biorxiv.org/content/10.1...
Special thanks to @aliceyting.bsky.social for discussions
December 19, 2024 at 2:22 AM
How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling.
www.biorxiv.org/content/10.1...
Special thanks to @aliceyting.bsky.social for discussions
www.biorxiv.org/content/10.1...
Special thanks to @aliceyting.bsky.social for discussions
Reposted by Alexandre Dumoulin
I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: authors.elsevier.com/sd/article/S... @cp-cell.bsky.social.
December 20, 2024 at 9:13 PM
I am super excited to share our article addressing a longstanding question in neurobiology: how Ca²⁺ signaling cascades initiated at dendritic spines operate at a distance. Check it out here: authors.elsevier.com/sd/article/S... @cp-cell.bsky.social.
For this #FluorescenceFriday, let's watch an axonal growth cone (for a change) displaying its most beautiful filopodia (this time)! 🤩 They are so dynamic! 1 image taken every 500 ms for 5 min played at 15 fps. 🧪
December 20, 2024 at 2:25 PM
For this #FluorescenceFriday, let's watch an axonal growth cone (for a change) displaying its most beautiful filopodia (this time)! 🤩 They are so dynamic! 1 image taken every 500 ms for 5 min played at 15 fps. 🧪
Reposted by Alexandre Dumoulin
I used VVD Viewer for this #FluorescenceFriday video: 141 volumes from the #HHMIJanelia FlyLight Split-GAL4 #Drosophila driver collection. The animation comes from a high-level description processed by neuVid. Such a rapid tour of the data has questionable scientific value, but it's fun to watch!
December 20, 2024 at 9:57 AM
I used VVD Viewer for this #FluorescenceFriday video: 141 volumes from the #HHMIJanelia FlyLight Split-GAL4 #Drosophila driver collection. The animation comes from a high-level description processed by neuVid. Such a rapid tour of the data has questionable scientific value, but it's fun to watch!
Reposted by Alexandre Dumoulin
Reposted by Alexandre Dumoulin
Lynn Landmesser was a giant in the field of developmental neurobiology. Sad to hear of her passing. #DevBio #neuroscience 🧪
I am saddened by the passing of Lynn Landmesser, a pioneer in neural development and cornerstone of our department. When I arrived, she was still teaching students to fabricate electrodes and dazzling junior faculty with stories about the history of the field. She will be missed.
December 13, 2024 at 2:59 PM
Lynn Landmesser was a giant in the field of developmental neurobiology. Sad to hear of her passing. #DevBio #neuroscience 🧪
This axonal growth cone from a culture of spinal neurons decided to display its most beautiful lamellipodia for this #FluorescenceFriday! 🤩 #neuroscience #liveimaging 🧪
1 image taken every second for 10 min, played at 15 images/s
1 image taken every second for 10 min, played at 15 images/s
December 13, 2024 at 8:43 AM
This axonal growth cone from a culture of spinal neurons decided to display its most beautiful lamellipodia for this #FluorescenceFriday! 🤩 #neuroscience #liveimaging 🧪
1 image taken every second for 10 min, played at 15 images/s
1 image taken every second for 10 min, played at 15 images/s
Reposted by Alexandre Dumoulin
Excellent preprint from @goodfrognosis.bsky.social’s
lab, showing convergence of #autism related proteins at the #cilium (sure to interest people working on cilia/centrosome on this platform; ping @cilialab.bsky.social)
www.biorxiv.org/content/10.1...
lab, showing convergence of #autism related proteins at the #cilium (sure to interest people working on cilia/centrosome on this platform; ping @cilialab.bsky.social)
www.biorxiv.org/content/10.1...
Convergence of autism proteins at the cilium
Hundreds of high confidence autism genes have been identified, yet the relevant etiological mechanisms remain unclear. Gene ontology analyses have repeatedly identified enrichment of proteins with ann...
www.biorxiv.org
December 9, 2024 at 1:31 PM
Excellent preprint from @goodfrognosis.bsky.social’s
lab, showing convergence of #autism related proteins at the #cilium (sure to interest people working on cilia/centrosome on this platform; ping @cilialab.bsky.social)
www.biorxiv.org/content/10.1...
lab, showing convergence of #autism related proteins at the #cilium (sure to interest people working on cilia/centrosome on this platform; ping @cilialab.bsky.social)
www.biorxiv.org/content/10.1...
Reposted by Alexandre Dumoulin
Come to be our colleague @pasteur.fr! center of Paris, great colleagues, outstanding facilities, permanent position, crème brûlée every Friday - what else could one want?
December 7, 2024 at 9:43 AM
Come to be our colleague @pasteur.fr! center of Paris, great colleagues, outstanding facilities, permanent position, crème brûlée every Friday - what else could one want?
Reposted by Alexandre Dumoulin
Viral tracing (yellow) in the mouse adult spinal cord (cyan, nuclei; magenta, axons). Image by the very talented Soizic Riche. #fluorescencefriday #fluorescentfriday
December 6, 2024 at 6:33 PM
Viral tracing (yellow) in the mouse adult spinal cord (cyan, nuclei; magenta, axons). Image by the very talented Soizic Riche. #fluorescencefriday #fluorescentfriday