Andy Moore
@aaandmoore.bsky.social
User of microscopes. Interested in organelles and how they move. Husband, dad, intermediate filament apologist, and postdoc in the JLS lab at HHMI Janelia Research Campus.
Reposted by Andy Moore
iPS cell-derived cardiac myocytes (heart muscle cells) typically beat about once per second, so I usually speed up the movies I post; otherwise, scrollers might miss the action. But every now and then, a cell looks like this in real time. #CellBiology
November 10, 2025 at 1:56 AM
iPS cell-derived cardiac myocytes (heart muscle cells) typically beat about once per second, so I usually speed up the movies I post; otherwise, scrollers might miss the action. But every now and then, a cell looks like this in real time. #CellBiology
www.nature.com/articles/s41...
My dad passed away in September. In addition to being a brilliant scientist, he was an accomplished fisherman, a scholar of 17th-century English silver, and my hero. I miss him terribly.
My dad passed away in September. In addition to being a brilliant scientist, he was an accomplished fisherman, a scholar of 17th-century English silver, and my hero. I miss him terribly.
In memoriam: Malcom A.S. Moore (1944–2025) - Leukemia
Leukemia - In memoriam: Malcom A.S. Moore (1944–2025)
www.nature.com
November 7, 2025 at 6:50 AM
www.nature.com/articles/s41...
My dad passed away in September. In addition to being a brilliant scientist, he was an accomplished fisherman, a scholar of 17th-century English silver, and my hero. I miss him terribly.
My dad passed away in September. In addition to being a brilliant scientist, he was an accomplished fisherman, a scholar of 17th-century English silver, and my hero. I miss him terribly.
Reposted by Andy Moore
A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...
Secretome translation shaped by lysosomes and lunapark-marked ER junctions - Nature
Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...
www.nature.com
November 6, 2025 at 2:24 PM
A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...
Reposted by Andy Moore
📕 Our Intermediate Filaments collection explores recent advances in intermediate filament research, focusing on lamins, keratins and vimentin. Explore new and recent JCB studies to accompany this week's European Intermediate Filament Meeting 👉 rupress.org/jcb/collecti...
#Euro-IF
#Euro-IF
September 23, 2025 at 2:15 PM
📕 Our Intermediate Filaments collection explores recent advances in intermediate filament research, focusing on lamins, keratins and vimentin. Explore new and recent JCB studies to accompany this week's European Intermediate Filament Meeting 👉 rupress.org/jcb/collecti...
#Euro-IF
#Euro-IF
Reposted by Andy Moore
Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...
Leaflet-specific phospholipid imaging using genetically encoded proximity sensors - Nature Chemical Biology
An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...
www.nature.com
September 15, 2025 at 6:03 PM
Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...
This is an airyscan confocal movie of mitochondria (white) moving around in a mouse astrocyte. Actin filaments are in orange and microtubules are in blue.
August 18, 2025 at 1:39 AM
This is an airyscan confocal movie of mitochondria (white) moving around in a mouse astrocyte. Actin filaments are in orange and microtubules are in blue.
Reposted by Andy Moore
Intermediate filaments join microtubules in the self-repair club! Great work with in vitro vimentin filaments from @cecileleduc.bsky.social @romet-jegou-lab.bsky.social et al. in @pnas.org: doi.org/10.1073/pnas...
Continuous self-repair protects vimentin intermediate filaments from fragmentation | PNAS
Intermediate filaments are key regulators of cell mechanics. Vimentin, a type of intermediate
filament expressed in mesenchymal cells and involved ...
doi.org
June 14, 2025 at 4:05 PM
Intermediate filaments join microtubules in the self-repair club! Great work with in vitro vimentin filaments from @cecileleduc.bsky.social @romet-jegou-lab.bsky.social et al. in @pnas.org: doi.org/10.1073/pnas...
This is a cumulative maximum intensity projection movie of the endoplasmic reticulum labeled with the membrane marker mEmerald-Sec61B.
June 11, 2025 at 12:55 AM
This is a cumulative maximum intensity projection movie of the endoplasmic reticulum labeled with the membrane marker mEmerald-Sec61B.
Reposted by Andy Moore
Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).
The latest AIC Call for Proposals is now open! Deadline is June 16, 2025. Come use @hhmijanelia.bsky.social imaging technology and expertise for FREE. Visit www.aicjanelia.org/apply for details. Before applying, contact us to schedule a technical consultation!
Apply | aicjanelia
Apply to use the advanced microscopes housed at the AIC.
www.aicjanelia.org
June 6, 2025 at 4:12 PM
Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).
Is it just me or do these cells look a lot like the americas?
May 30, 2025 at 11:26 PM
Is it just me or do these cells look a lot like the americas?
Reposted by Andy Moore
In the context of our @reviewcommons.org revision process, I'm happy to announce Microscopy Nodes v2.2.0!
This packs lots of new fun features, including new color management 🌈, clearer transparency handling 🫥, custom default settings 🔧 and more!
Preprint at doi.org/10.1101/2025...
This packs lots of new fun features, including new color management 🌈, clearer transparency handling 🫥, custom default settings 🔧 and more!
Preprint at doi.org/10.1101/2025...
May 23, 2025 at 1:13 PM
In the context of our @reviewcommons.org revision process, I'm happy to announce Microscopy Nodes v2.2.0!
This packs lots of new fun features, including new color management 🌈, clearer transparency handling 🫥, custom default settings 🔧 and more!
Preprint at doi.org/10.1101/2025...
This packs lots of new fun features, including new color management 🌈, clearer transparency handling 🫥, custom default settings 🔧 and more!
Preprint at doi.org/10.1101/2025...
Reposted by Andy Moore
📣 We’re thrilled to announce the judging panel for the 2025 #NikonSmallWorld photomicrography and Small World in Motion video competitions!
🔗 Click to learn about this year's judges: bit.ly/43hmGBq
#Microscopy #Imaging #SciComm #SciArt
🔗 Click to learn about this year's judges: bit.ly/43hmGBq
#Microscopy #Imaging #SciComm #SciArt
May 14, 2025 at 11:08 PM
📣 We’re thrilled to announce the judging panel for the 2025 #NikonSmallWorld photomicrography and Small World in Motion video competitions!
🔗 Click to learn about this year's judges: bit.ly/43hmGBq
#Microscopy #Imaging #SciComm #SciArt
🔗 Click to learn about this year's judges: bit.ly/43hmGBq
#Microscopy #Imaging #SciComm #SciArt
FilaBuster - Vimentin IF fragmentation
May 25, 2025 at 3:47 AM
FilaBuster - Vimentin IF fragmentation
Reposted by Andy Moore
In the classical view of mitotic cell division, the spindle apparatus maintains principal control of chromosome capture and alignment. Any breakdown in spindle function can result in chromosome mis-segregation, producing daughter cells with abnormal chromosome number.
May 23, 2025 at 7:57 PM
In the classical view of mitotic cell division, the spindle apparatus maintains principal control of chromosome capture and alignment. Any breakdown in spindle function can result in chromosome mis-segregation, producing daughter cells with abnormal chromosome number.
Reposted by Andy Moore
I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)
May 23, 2025 at 7:57 PM
I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)
Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 22, 2025 at 1:02 AM
Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Andy Moore
FilaBuster: A Strategy for Rapid, Specific, and Spatiotemporally Controlled Intermediate Filament Disassembly https://www.biorxiv.org/content/10.1101/2025.04.20.649718v1
April 21, 2025 at 10:30 PM
FilaBuster: A Strategy for Rapid, Specific, and Spatiotemporally Controlled Intermediate Filament Disassembly https://www.biorxiv.org/content/10.1101/2025.04.20.649718v1
What started as a brief chat with @buvansr.bsky.social and @vgelfand.bsky.social at the 2023 CellBio meeting @ascbiology.bsky.social has grown into our published collaborative work on single vimentin IF dynamics and organization in cells. Love when stuff like that happens.
Renganathan, Moor, @vgelfand.bsky.social et al. use single-particle tracking to show that #vimentin intermediate filaments display active movement throughout the cell, with individual filaments within a bundle moving independently rupress.org/jcb/article/...
#Cytoskeleton #Microscopy
#Cytoskeleton #Microscopy
March 10, 2025 at 6:39 PM
What started as a brief chat with @buvansr.bsky.social and @vgelfand.bsky.social at the 2023 CellBio meeting @ascbiology.bsky.social has grown into our published collaborative work on single vimentin IF dynamics and organization in cells. Love when stuff like that happens.
Reposted by Andy Moore
Challenging the view on #vimentin! our latest work shows VIF is an active, dynamic filament loosely held together
rupress.org/jcb/article/...
Thanks to @vgelfand.bsky.social @aaandmoore.bsky.social @nu-bsa.bsky.social @hhmijanelia.bsky.social @jcb.org #NUCDB #nikon #CrestOptics #livecellimaging
rupress.org/jcb/article/...
Thanks to @vgelfand.bsky.social @aaandmoore.bsky.social @nu-bsa.bsky.social @hhmijanelia.bsky.social @jcb.org #NUCDB #nikon #CrestOptics #livecellimaging
March 10, 2025 at 4:38 PM
Challenging the view on #vimentin! our latest work shows VIF is an active, dynamic filament loosely held together
rupress.org/jcb/article/...
Thanks to @vgelfand.bsky.social @aaandmoore.bsky.social @nu-bsa.bsky.social @hhmijanelia.bsky.social @jcb.org #NUCDB #nikon #CrestOptics #livecellimaging
rupress.org/jcb/article/...
Thanks to @vgelfand.bsky.social @aaandmoore.bsky.social @nu-bsa.bsky.social @hhmijanelia.bsky.social @jcb.org #NUCDB #nikon #CrestOptics #livecellimaging
Reposted by Andy Moore
Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM rupress.org/jcb/article-...
Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM
Vimentin intermediate filaments display active movement throughout the cell, with individual filaments within a bundle moving independently, as demonstrate
rupress.org
March 10, 2025 at 2:27 PM
Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM rupress.org/jcb/article-...
Poor little vimentin stick man 😱
A couple of months later than expected, but I'm finally gearing up to share *FilaBuster* - our approach for light-mediated IF disassembly. Stay tuned!
A couple of months later than expected, but I'm finally gearing up to share *FilaBuster* - our approach for light-mediated IF disassembly. Stay tuned!
March 10, 2025 at 12:53 AM
Poor little vimentin stick man 😱
A couple of months later than expected, but I'm finally gearing up to share *FilaBuster* - our approach for light-mediated IF disassembly. Stay tuned!
A couple of months later than expected, but I'm finally gearing up to share *FilaBuster* - our approach for light-mediated IF disassembly. Stay tuned!
Vimentin (orange) and actin filaments (gray) in a COS-7. Airyscan images of the cytoskeleton always bring me joy. I square root transformed the vimentin so you can see the dimmer filaments out at the edge.
March 4, 2025 at 5:10 AM
Vimentin (orange) and actin filaments (gray) in a COS-7. Airyscan images of the cytoskeleton always bring me joy. I square root transformed the vimentin so you can see the dimmer filaments out at the edge.
Reposted by Andy Moore
Hey #chemsky and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?
March 1, 2025 at 1:57 PM
Hey #chemsky and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?
Reposted by Andy Moore
The N-SIM scope got its annual visit at @ncis-marseille.bsky.social and it's ready to deliver! Projected 3D-SIM stack of a COS cell labeled for actin ⚪ microtubules 🔵 and clathrin 🟠
February 12, 2025 at 12:00 PM
The N-SIM scope got its annual visit at @ncis-marseille.bsky.social and it's ready to deliver! Projected 3D-SIM stack of a COS cell labeled for actin ⚪ microtubules 🔵 and clathrin 🟠