Thanks! This was actually all dyes. Mito tracker green, SPY555-Tubulin, and SPY650-FastAct-X.

Whoops forgot to add that - 5 microns

It’s sped up - the timer is minutes:seconds

I double checked the metadata and I’m 90% sure it’s not the sun.

Zeiss 880 w/ airyscan

Not discussed so much but those tubules pressed up against the nuclear envelope are certainly unique.

Thank you! This is an airyscan stack.

Thank you! Yup, good old COS-7.

This is a HeLa

Close! This is a HeLa expressing lifeact-egfp. It’s an odd looking hela to be fair

In the classical view of mitotic cell division, the spindle apparatus maintains principal control of chromosome capture and alignment. Any breakdown in spindle function can result in chromosome mis-segregation, producing daughter cells with abnormal chromosome number.