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aicjanelia.bsky.social
AIC at Janelia
@aicjanelia.bsky.social
850 followers 91 following 37 posts
Venture of HHMI to make pre-commercial microscopes available to a broad audience. Learn more at https://www.aicjanelia.org/
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Reposted by AIC at Janelia
stevenssostre.bsky.social
I learned so much from the @aicjanelia.bsky.social team at the #InsightFromImaging 2025 workshop this past week. Thank you so much! It was so fun and informative. I enjoyed the discussions between #HannaGrayFellows and the AIC team at @hhmijanelia.bsky.social. I’m off to do bioimaging experiments! 🔬
November 8, 2025 at 4:31 PM
aicjanelia.bsky.social
One last Bob's coffee break at @hhmijanelia.bsky.social before our final #InsightFromImaging activity: Participants are working in groups to formulate a hypothesis and design an imaging experiment to test it 🔬
A photo of a coffee mug. The mug is labeled with writing that says "hhmi | janelia Research Campus." The drink in the mug has the words "Insight From Imaging 2025" printed on the foam.
November 7, 2025 at 9:37 PM
aicjanelia.bsky.social
For our final lecture at #InsightFromImaging 2025, @jesseaic.bsky.social is discussing electron microscopy and correlative approaches, including results from our FIB-SEM system, which can achieve 4 nm resolution in 3D!
November 7, 2025 at 4:36 PM
aicjanelia.bsky.social
We spent the afternoon at #InsightFromImaging in the lab exploring 5 different microscopes (& 5 different samples!) to understand approaches for imaging depth.

Kidney slice prep by Ellen, imaged by Jenny using 2P (green tubulin) + SHG (magenta), viz by @scientistrachel.bsky.social in #AGAVE
A 3D rendering of microscopy data. Fluorescently labeled tubulin is shown in green, while second harmonic generation is shown in magenta.
November 6, 2025 at 10:20 PM
aicjanelia.bsky.social
Today at #InsightFromImaging we're focusing on imaging depth, starting with a lecture by @cmhobson.bsky.social. Adaptive optics (available on our MOSAIC system!) can improve deep imaging, as shown by these mitochondria in a zebrafish embryo.
A microscopy image with text annotation. The left side of the image is labeled "System Correction Only" and shows blurry and aberrated mitochondria. The right side of the image is labeled "Adaptive Optics + Deconvolution" and shows a clearer image of mitochondria.
November 6, 2025 at 2:53 PM
aicjanelia.bsky.social
This morning at #InsightFromImaging we focused on particle tracking. After a lecture by @jesseaic.bsky.social, participants split into groups to use #TrackMate to track our HL60 migration data from yesterday, followed by presentations on their results 📈
A collage of photos, which show people analyzing images and giving presentations.
November 5, 2025 at 8:05 PM
Reposted by AIC at Janelia
scientistrachel.bsky.social
Yes, they are the same field of view 😄 Bonus additional #InsightFromImaging cell from a different FOV here:
November 5, 2025 at 2:13 PM
Reposted by AIC at Janelia
scientistrachel.bsky.social
HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social
November 4, 2025 at 10:36 PM
aicjanelia.bsky.social
Continuing our speed theme at #InsightFromImaging, we spent the afternoon at the microscope exploring imaging speed, as well as tradeoffs in imaging scale. Video featuring imaging of neutrophil-like HL60 cells on a widefield vs the AIC's LLSM.
November 4, 2025 at 10:33 PM
aicjanelia.bsky.social
Today's theme at #InsightFromImaging 2025 is imaging speed. We're kicking things off with a lecture by Chad Hobson.
A photo of a classroom. A man stands at the podium next to a projector screen. The audience sits at tables in front of the screen.
November 4, 2025 at 4:33 PM
aicjanelia.bsky.social
For our first #InsightFromImaging lab, we're exploring imaging resolution across a variety of microscopy systems. When is increased resolution really necessary? What trade-offs does it require?
A microscopy image of a cell labeled for actin (magenta) and paxillin (green). The left half of the image is taken in widefield mode, while the right half is taken in confocal mode. The scale bar is 10 um.
November 3, 2025 at 10:31 PM
Reposted by AIC at Janelia
scientistrachel.bsky.social
I had a wonderful time discussing image analysis with HHMI #HannaGrayFellows this morning. I look forward to continuing the chat throughout #InsightFromImaging this week.
aicjanelia.bsky.social AIC at Janelia @aicjanelia.bsky.social · 20d
This morning at #InsightFromImaging we jumped into hands on image analysis activities, including comparing the pros and cons of imaging denoising approaches
A photo of a classroom. There is a projector screen at the front of the room, and participants sit at tables with laptops performing image analysis tasks.
November 3, 2025 at 6:05 PM
aicjanelia.bsky.social
This morning at #InsightFromImaging we jumped into hands on image analysis activities, including comparing the pros and cons of imaging denoising approaches
A photo of a classroom. There is a projector screen at the front of the room, and participants sit at tables with laptops performing image analysis tasks.
November 3, 2025 at 6:02 PM
aicjanelia.bsky.social
We're kicking off #InsightFromImaging 2025 with a keynote lecture by @scopeshifu.bsky.social on advanced microscopy and the resources available through the Advanced Imaging Center's visitor program 🔬
A photo of a man giving a presentation. He is standing in front of a projector screen. In the front of the photo, the audience sits at long tables.
November 2, 2025 at 10:28 PM
Reposted by AIC at Janelia
scientistrachel.bsky.social
When #FluorescenceFriday falls on Halloween, we break out the spooky data 👻 Here we are looking at the blood vessels of a zebrafish through its eye 🎃
A 3D rendering of microscopy data. The structure of a fish is visible in gray while green blood vessels are visible through the eye.
October 31, 2025 at 3:29 PM
Reposted by AIC at Janelia
nicolasdenans.bsky.social
For #SpookyMicroscopyFriday I present the mugshot of the head of the zebrafish mafia
October 31, 2025 at 12:49 PM
aicjanelia.bsky.social
Wishing everyone a happy #FluorescenceFriday and a #HappyHalloween! This expansion microscopy visualization of blood vessels in a zebrafish is the result of a collaboration between Ellen, Jenny, @nicolasdenans.bsky.social, and @scientistrachel.bsky.social.
October 31, 2025 at 3:35 PM
aicjanelia.bsky.social
Our team is hard at work preparing for #InsightFromImaging 2025! We can't wait to welcome HHMI #HannaGrayFellows to @hhmijanelia.bsky.social next week for lectures, hands-on labs, and activities focused on designing informative and quantitative bioimaging experiments 🔬
A microscopy image of a cell stained for actin (magneta color) and focal adhesions (green color).
October 27, 2025 at 5:24 PM
aicjanelia.bsky.social
Today we're wrapping up a flatworm imaging project on our SiMView microscope. We've enjoyed having @kinglhung.bsky.social and team from the lab of @ardemp.bskyverified.social here and look forward to working with them on analyzing their images!
A photo of two rows of people. The first row of people is sitting on bench while the second row stands behind them, in front of floor to ceiling windows. Outside the windows is a fall scene with trees.
October 10, 2025 at 4:16 PM
aicjanelia.bsky.social
Today we're wrapping up 3 weeks of zebrafish imaging on our MOSAIC system. We've enjoyed having Lisa and Miray of @annamolofskylab.bsky.social here and wish them safe travels home!
A photo of a group of people standing in front of scientific equipment.
September 26, 2025 at 8:31 PM
aicjanelia.bsky.social
Today we're wrapping up an iPALM visit with @anirban-banerjee.bsky.social, @souravsgw.bsky.social, and team. Wishing them safe travels home!
A photo of a group of people. A group stands in front of the wall, while two women sit in chairs in front of them.
September 5, 2025 at 8:12 PM
aicjanelia.bsky.social
We've enjoyed having Marise and Samara from the lab of @melaniedwhite.bsky.social here to image quail embryos on our MOSAIC system. We wish them safe travels home and happy data analyzing!
A photo of a group of five people standing in a room filled with scientific equipment.
August 8, 2025 at 8:47 PM
aicjanelia.bsky.social
We are wrapping up a project imaging cancer cell spheroids on our SiMView system today. We wish Elizabeta Belova of @temple.edu happy data analyzing and safe travels home!
A photo of a group of people. Two people are sitting in chairs with a small table between them, while the remaining four people stand behind the chairs. The white wall behind the group is decorated with microscopy images.
July 25, 2025 at 5:03 PM
Reposted by AIC at Janelia
melaniedwhite.bsky.social
Today was a super fun first day of imaging @aicjanelia.bsky.social with @vanderspuy.bsky.social and @samarar.bsky.social.
All hail the quail!🧪🔬#DevBio
July 21, 2025 at 11:44 PM
aicjanelia.bsky.social
Today we're wrapping up a dicty project on our MOSAIC system. We've enjoyed having Yiyan and Saki of the Devreotes lab here and wish them happy data analyzing!
A group of four people stand in front of a microscope system. They are surrounded by scientific equipment, including two large computer monitors.
July 11, 2025 at 12:46 PM