Rachel Lee, PhD
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Rachel Lee, PhD
@scientistrachel.bsky.social
BioImage Analysis Lead at AIC Janelia
Reposted by Rachel Lee, PhD
I learned so much from the @aicjanelia.bsky.social team at the #InsightFromImaging 2025 workshop this past week. Thank you so much! It was so fun and informative. I enjoyed the discussions between #HannaGrayFellows and the AIC team at @hhmijanelia.bsky.social. I’m off to do bioimaging experiments! 🔬
November 8, 2025 at 4:31 PM
Reposted by Rachel Lee, PhD
One last Bob's coffee break at @hhmijanelia.bsky.social before our final #InsightFromImaging activity: Participants are working in groups to formulate a hypothesis and design an imaging experiment to test it 🔬
November 7, 2025 at 9:37 PM
Reposted by Rachel Lee, PhD
We spent the afternoon at #InsightFromImaging in the lab exploring 5 different microscopes (& 5 different samples!) to understand approaches for imaging depth.

Kidney slice prep by Ellen, imaged by Jenny using 2P (green tubulin) + SHG (magenta), viz by @scientistrachel.bsky.social in #AGAVE
November 6, 2025 at 10:20 PM
Reposted by Rachel Lee, PhD
Today at #InsightFromImaging we're focusing on imaging depth, starting with a lecture by @cmhobson.bsky.social. Adaptive optics (available on our MOSAIC system!) can improve deep imaging, as shown by these mitochondria in a zebrafish embryo.
November 6, 2025 at 2:53 PM
Working with #TrackMate, participants at #InsightFromImaging were able to explore tracking parameters and the tradeoffs inherent in analysis. It was another fun discussion! 🤓
This morning at #InsightFromImaging we focused on particle tracking. After a lecture by @jesseaic.bsky.social, participants split into groups to use #TrackMate to track our HL60 migration data from yesterday, followed by presentations on their results 📈
November 5, 2025 at 8:10 PM
HL60 cells take on so many fun shapes as they migrate! This #InsightFromImaging data features cells prepared by Leanna and imaged on the @aicjanelia.bsky.social LLSM by @cmhobson.bsky.social
November 4, 2025 at 10:36 PM
Reposted by Rachel Lee, PhD
Continuing our speed theme at #InsightFromImaging, we spent the afternoon at the microscope exploring imaging speed, as well as tradeoffs in imaging scale. Video featuring imaging of neutrophil-like HL60 cells on a widefield vs the AIC's LLSM.
November 4, 2025 at 10:33 PM
Reposted by Rachel Lee, PhD
Today's theme at #InsightFromImaging 2025 is imaging speed. We're kicking things off with a lecture by Chad Hobson.
November 4, 2025 at 4:33 PM
Reposted by Rachel Lee, PhD
For our first #InsightFromImaging lab, we're exploring imaging resolution across a variety of microscopy systems. When is increased resolution really necessary? What trade-offs does it require?
November 3, 2025 at 10:31 PM
I had a wonderful time discussing image analysis with HHMI #HannaGrayFellows this morning. I look forward to continuing the chat throughout #InsightFromImaging this week.
This morning at #InsightFromImaging we jumped into hands on image analysis activities, including comparing the pros and cons of imaging denoising approaches
November 3, 2025 at 6:05 PM
Reposted by Rachel Lee, PhD
We're kicking off #InsightFromImaging 2025 with a keynote lecture by @scopeshifu.bsky.social on advanced microscopy and the resources available through the Advanced Imaging Center's visitor program 🔬
November 2, 2025 at 10:28 PM
Reposted by Rachel Lee, PhD
October 31, 2025 at 4:21 PM
Reposted by Rachel Lee, PhD
Wishing everyone a happy #FluorescenceFriday and a #HappyHalloween! This expansion microscopy visualization of blood vessels in a zebrafish is the result of a collaboration between Ellen, Jenny, @nicolasdenans.bsky.social, and @scientistrachel.bsky.social.
October 31, 2025 at 3:35 PM
Reposted by Rachel Lee, PhD
Hippocampus masquerading as a 🦇 for Halloween 🎃
#FluorescenceFriday

Image credit to the @lieberinstitute.bsky.social Microscopy and Spatial Biology Core 🧠🔬🧪
October 31, 2025 at 12:31 PM
When #FluorescenceFriday falls on Halloween, we break out the spooky data 👻 Here we are looking at the blood vessels of a zebrafish through its eye 🎃
October 31, 2025 at 3:29 PM
Reposted by Rachel Lee, PhD
A halloween cartoon for @newscientist.com
October 31, 2025 at 11:27 AM
I'm excited to be co-organizing #InsightFromImaging 2025! We have many interactive activities planned, including hands-on image analysis labs 🤓
Our team is hard at work preparing for #InsightFromImaging 2025! We can't wait to welcome HHMI #HannaGrayFellows to @hhmijanelia.bsky.social next week for lectures, hands-on labs, and activities focused on designing informative and quantitative bioimaging experiments 🔬
October 27, 2025 at 5:28 PM
Today marks 4 years of enjoying the beautiful @hhmijanelia.bsky.social campus 🌳
October 12, 2025 at 12:17 AM
Reposted by Rachel Lee, PhD
Today we're wrapping up a flatworm imaging project on our SiMView microscope. We've enjoyed having @kinglhung.bsky.social and team from the lab of @ardemp.bskyverified.social here and look forward to working with them on analyzing their images!
October 10, 2025 at 4:16 PM
Reposted by Rachel Lee, PhD
Who does microscopy and has never heard of Bio-Formats? You probably used it anyway
June 4, 2025 at 9:13 PM
Reposted by Rachel Lee, PhD
We've enjoyed having Marise and Samara from the lab of @melaniedwhite.bsky.social here to image quail embryos on our MOSAIC system. We wish them safe travels home and happy data analyzing!
August 8, 2025 at 8:47 PM
Reposted by Rachel Lee, PhD
Google search's AI summary is like the loud confident idiot in the room who doesn't always know the answer to your question but HAS TO be the first one to reply to it.
July 28, 2025 at 12:17 PM
Reposted by Rachel Lee, PhD
The Confocal Room
July 30, 2025 at 10:49 AM
Reposted by Rachel Lee, PhD
Today was a super fun first day of imaging @aicjanelia.bsky.social with @vanderspuy.bsky.social and @samarar.bsky.social.
All hail the quail!🧪🔬#DevBio
July 21, 2025 at 11:44 PM
Reposted by Rachel Lee, PhD
We are wrapping up a project imaging cancer cell spheroids on our SiMView system today. We wish Elizabeta Belova of @temple.edu happy data analyzing and safe travels home!
July 25, 2025 at 5:03 PM