Spirochrome
@spirochrome.com
Our main focus is to offer innovative tools for live cell fluorescence imaging to the scientific community.
https://spirochrome.com/
https://spirochrome.com/
A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
November 24, 2025 at 1:34 PM
A new article from Nicolas Plachta's lab with incredible imaging of live human embryos using our probes SPY555-DNA and SPY650-FastAct
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
www.science.org/doi/full/10....
The study shows that frequent errors during cell division directly cause many early developmental failures and morphological defects
Reposted by Spirochrome
Cool, the HAK-actin preprint was showcased by @prelights.bsky.social
A good reason to read it if you haven't yet 😜
A good reason to read it if you haven't yet 😜
Reliable actin labelling in expansion #microscopy 🔬
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton - preLights
HAK-actin is a new probe that may help researchers see the actin cytoskeleton in sharper detail using expansion microscopy.
prelights.biologists.com
November 19, 2025 at 12:28 PM
Cool, the HAK-actin preprint was showcased by @prelights.bsky.social
A good reason to read it if you haven't yet 😜
A good reason to read it if you haven't yet 😜
Reposted by Spirochrome
Quite a busy day on #Bluesky !
Been a while since we have been #preLight -ed !
Discover HAK-Actin if you haven't already ;)
Been a while since we have been #preLight -ed !
Discover HAK-Actin if you haven't already ;)
Reliable actin labelling in expansion #microscopy 🔬
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton - preLights
HAK-actin is a new probe that may help researchers see the actin cytoskeleton in sharper detail using expansion microscopy.
prelights.biologists.com
November 19, 2025 at 12:36 PM
Thank you @prelights.bsky.social for highlighting HAK-actin!
HAK-actin is a great tool for ExM actin staining.
spirochrome.com/product/hak-...
HAK-actin is a great tool for ExM actin staining.
spirochrome.com/product/hak-...
Reliable actin labelling in expansion #microscopy 🔬
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social
Read the latest #preLight from @kanishka03.bsky.social ⬇️👀
prelights.biologists.com/highlights/h...
HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton - preLights
HAK-actin is a new probe that may help researchers see the actin cytoskeleton in sharper detail using expansion microscopy.
prelights.biologists.com
November 20, 2025 at 9:54 AM
Thank you @prelights.bsky.social for highlighting HAK-actin!
HAK-actin is a great tool for ExM actin staining.
spirochrome.com/product/hak-...
HAK-actin is a great tool for ExM actin staining.
spirochrome.com/product/hak-...
Reposted by Spirochrome
Klein Scientific telling us about the products they distribute including @spirochrome.com products. @globalbioimaging.bsky.social #GBI_AU_2025
November 18, 2025 at 4:09 AM
Klein Scientific telling us about the products they distribute including @spirochrome.com products. @globalbioimaging.bsky.social #GBI_AU_2025
👉🦠Interested in bacterial membrane tension?
Our membrane tension probe Flipper-TR is a top tool for the job!
A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.
🔗 doi.org/10.1073/pnas...
🧪
Our membrane tension probe Flipper-TR is a top tool for the job!
A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.
🔗 doi.org/10.1073/pnas...
🧪
Exploring bacteria–surface interactions with a fluorescent membrane tension probe | PNAS
Understanding how bacteria interact with surfaces is critical for advancing applications
in biofilm and biofouling prevention, biomaterial developm...
doi.org
November 4, 2025 at 9:19 AM
👉🦠Interested in bacterial membrane tension?
Our membrane tension probe Flipper-TR is a top tool for the job!
A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.
🔗 doi.org/10.1073/pnas...
🧪
Our membrane tension probe Flipper-TR is a top tool for the job!
A new article in @pnas.org demonstrates how Flipper-TR efficiently stains bacterial membranes and responds to various stimuli altering membrane tension.
🔗 doi.org/10.1073/pnas...
🧪
Reposted by Spirochrome
October 29, 2025 at 9:30 AM
Reposted by Spirochrome
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
October 29, 2025 at 9:30 AM
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
October 29, 2025 at 9:30 AM
Live cell ACTIN imaging has never been easier with SPY650-FastAct_X!
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
1. Add the probe to the cells
2. Image 1h later
SPY650 is highly suited for STED imaging. The image shows a confocal/STED comparison of the fine actin mesh in a live HeLa cell.
image credits: @stephanlab.bsky.social
An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
🚨 New preprint out!
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
“Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM” with Tithi Mandal and @rouxlab.bsky.social
We benchmark and describe step-by-step Flipper-TR FLIM measurements across different set-ups and and strategies.
www.biorxiv.org/content/10.1...
Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM
Flipper-TR is a membrane dye sensitive to lipid packing widely used to probe membrane tension in live cells via fluorescence lifetime imaging microscopy (FLIM). However, no consensus currently exists ...
www.biorxiv.org
October 7, 2025 at 8:21 AM
An incredibly comprehensive and meticulous study of Flipper-TR lifetime analysis to make your membrane tension measurements using Flipper-TR more robust and reliable.
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
Thanks to @juanmagararc.bsky.social and @rouxlab.bsky.social for this very valuable resource!
Reposted by Spirochrome
📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
October 2, 2025 at 8:40 AM
📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
October 2, 2025 at 8:40 AM
📢📄 A new preprint by @katecavanaugh.bsky.social links reduced fertility stemming from impaired implantation from older mothers to embryo hypercontractility
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
SPY650-FastAct was the key probe to track actin dynamics in live embryos implantation movies over 24 h long 🤯
doi.org/10.1101/2025...
#imaging
Reposted by Spirochrome
SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N
September 17, 2025 at 5:20 PM
SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N
Reposted by Spirochrome
Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪
Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition!
His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.
www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.
www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
In vitro growth of chick sensory neurons | 2025 Small World in Motion Competition
Alexandre Dumoulin - In vitro growth of chick sensory neurons, shown in gold using a dye marking their internal structure (18 hour time lapse)
www.nikonsmallworld.com
September 29, 2025 at 12:46 PM
Very happy to share that my latest submission to the #NikonSmallWorld in Motion competition received an Honorable Mention this year! 🥳 #neuroscience 🧪
Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition!
His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.
www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.
www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
In vitro growth of chick sensory neurons | 2025 Small World in Motion Competition
Alexandre Dumoulin - In vitro growth of chick sensory neurons, shown in gold using a dye marking their internal structure (18 hour time lapse)
www.nikonsmallworld.com
September 26, 2025 at 8:59 AM
Huge congratulations to @axonsalex.bsky.social for his Honorable mention a the #NikonSmallWorld in Motion competition!
His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.
www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
His movie shows axonal growth of chicken dorsal root ganglia neurons using our SPY555-FastAct probe to stain actin.
www.nikonsmallworld.com/galleries/20...
#FluorescenceFriday
Reposted by Spirochrome
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
September 19, 2025 at 9:45 AM
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
September 19, 2025 at 9:45 AM
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Amazing achievement!
Congratulations
Cherry on the cake, it's SPY555-Actin staining the actin cytoskeleton in the video 😍
Congratulations
Cherry on the cake, it's SPY555-Actin staining the actin cytoskeleton in the video 😍
SCHEPHERD--the bioelectric cell herding platform built for YOU. Single cells, monolayers, organoids--this herds them all + new tricks. Plz try it-- we will *give* you parts! Teaser here of a steering a single cell. GS Yubin Lin's lifeblood with J. Yodh on piano; Celeste R. and Paul K. Thread 1/N
September 18, 2025 at 7:02 PM
Amazing achievement!
Congratulations
Cherry on the cake, it's SPY555-Actin staining the actin cytoskeleton in the video 😍
Congratulations
Cherry on the cake, it's SPY555-Actin staining the actin cytoskeleton in the video 😍
Reposted by Spirochrome
🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
Multispectral live-cell imaging with uncompromised spatiotemporal resolution - Nature Photonics
A tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with...
www.nature.com
September 16, 2025 at 10:20 AM
🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
Reposted by Spirochrome
📄 🌟 Researchers introduce HAK-actin, an actin label compatible with post-labeling in #SuperResolution ultrastructure expansion #Microscopy #ExM. The authors demonstrate its use in cells expanded up to 16-fold (figure).
🔗 View the preprint article: bit.ly/47xC8NJ
#Microscope #Imaging #Fluorescence
🔗 View the preprint article: bit.ly/47xC8NJ
#Microscope #Imaging #Fluorescence
September 10, 2025 at 1:41 PM
📄 🌟 Researchers introduce HAK-actin, an actin label compatible with post-labeling in #SuperResolution ultrastructure expansion #Microscopy #ExM. The authors demonstrate its use in cells expanded up to 16-fold (figure).
🔗 View the preprint article: bit.ly/47xC8NJ
#Microscope #Imaging #Fluorescence
🔗 View the preprint article: bit.ly/47xC8NJ
#Microscope #Imaging #Fluorescence
Reposted by Spirochrome
For all of you #Actin folks ... It's out !
Happy to help at anypoint
Happy to help at anypoint
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
September 10, 2025 at 9:11 AM
For all of you #Actin folks ... It's out !
Happy to help at anypoint
Happy to help at anypoint
Reposted by Spirochrome
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
September 10, 2025 at 7:58 AM
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
Reposted by Spirochrome
🧬 New tool for U-ExM!
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
September 10, 2025 at 9:59 AM
🧬 New tool for U-ExM!
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
September 10, 2025 at 7:58 AM
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
A stunning application of SPY650-FastAct_X (actin)/SPY555-DNA (nuclei) staining iPSC cells and imaged by light sheet microscopy at UCSF. Thank you @microtubule.bsky.social for sharing it!
Some first raw (not deskewed) data of live human induced pluripotent stem cells from #Snouty now ready for some serious live imaging. Light sheet moving at a 38-degree angle through the cell layer; coverslip is on the bottom.
September 2, 2025 at 7:07 AM
A stunning application of SPY650-FastAct_X (actin)/SPY555-DNA (nuclei) staining iPSC cells and imaged by light sheet microscopy at UCSF. Thank you @microtubule.bsky.social for sharing it!