Akaash Kumar
@akaashkumar.bsky.social
Interdisciplinary scientist interested in technology development, microscopy and biological sciences.
Multispectral live cell imaging of membrane trafficking.
MRC LMB, Cambridge, UK.
Multispectral live cell imaging of membrane trafficking.
MRC LMB, Cambridge, UK.
Pinned
Akaash Kumar
@akaashkumar.bsky.social
· Nov 20
In future work, I am now using our multispectral technology to study membrane trafficking pathways in cytotoxic T cells and related immunotherapies, to decode how T cells are able to eliminate virally-infected/cancer cells and what happens in patients when these processes go wrong. Updates to come!
September 16, 2025 at 10:20 AM
In future work, I am now using our multispectral technology to study membrane trafficking pathways in cytotoxic T cells and related immunotherapies, to decode how T cells are able to eliminate virally-infected/cancer cells and what happens in patients when these processes go wrong. Updates to come!
We demonstrated the ability to simultaneously image trafficking of five different endogenous receptor classes using de-novo binders from David Baker's lab, by adding the multispectral technology to a state-of-the-art single objective light sheet microscope, capturing data at 3 volumes per second.
September 16, 2025 at 10:20 AM
We demonstrated the ability to simultaneously image trafficking of five different endogenous receptor classes using de-novo binders from David Baker's lab, by adding the multispectral technology to a state-of-the-art single objective light sheet microscope, capturing data at 3 volumes per second.
The technology we developed is compatible with any camera-based microscope and by using polycistronic plasmids and live-cell dyes (e.g. @spirochrome.com) we can image multiple organelles in live cells simultaneously.
September 16, 2025 at 10:20 AM
The technology we developed is compatible with any camera-based microscope and by using polycistronic plasmids and live-cell dyes (e.g. @spirochrome.com) we can image multiple organelles in live cells simultaneously.
🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
Multispectral live-cell imaging with uncompromised spatiotemporal resolution - Nature Photonics
A tree-like arrangement of dichroic mirrors and multiple cameras coupled with an iterative spectral unmixing algorithm enables multispectral imaging of live cells in up to eight spectral channels with...
www.nature.com
September 16, 2025 at 10:20 AM
🚨Publication alert🚨
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
My first, first-author paper is now out in @natphoton.nature.com! Our paper describes an iterative spectral unmixing algorithm and eight-channel camera-based hardware we developed enabling unmixing of low SNR live-cell data at video rates. www.nature.com/articles/s41...
Reposted by Akaash Kumar
We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:
September 15, 2025 at 8:42 AM
We present a simple method to easily increase the imageable depth of an expansion microscopy gel on a typical inverted microscope ten-fold, using some carefully placed FEP film and a water dipping objective lens:
Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!
May 20, 2025 at 6:21 PM
Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!
Thank you very much for the feature and the article!! A nice surprise before the weekend...
New approach with an unmixing algorithm & 8-channel image acquisition enables multispectral live-cell imaging
📷: @akaashkumar.bsky.social et al
@jamesdmanton.bsky.social @cellbiol-mrclmb.bsky.social @mrclmb.bsky.social in @biorxivpreprint.bsky.social
➡️: bpod.org.uk/archive/2025... with
Ant Lewis
📷: @akaashkumar.bsky.social et al
@jamesdmanton.bsky.social @cellbiol-mrclmb.bsky.social @mrclmb.bsky.social in @biorxivpreprint.bsky.social
➡️: bpod.org.uk/archive/2025... with
Ant Lewis
February 14, 2025 at 11:11 AM
Thank you very much for the feature and the article!! A nice surprise before the weekend...
Congratulations @lara-kruger.bsky.social ! We wish you all the best!
Lets goo (very soon)...
Merci beaucoup to the exquisite @deriverylab.bsky.social at the amazing @mrclmb.bsky.social. I had a lovely, lovely time!
Merci beaucoup to the exquisite @deriverylab.bsky.social at the amazing @mrclmb.bsky.social. I had a lovely, lovely time!
Congratulations to @lara-kruger.bsky.social, postdoc in @deriverylab.bsky.social in @cellbiol-mrclmb.bsky.social, who is leaving the LMB to establish her own research group at @institutcurie.bsky.social in Paris!
Best of luck to you Lara!
#LMBAlumni
Best of luck to you Lara!
#LMBAlumni
February 7, 2025 at 12:59 PM
Congratulations @lara-kruger.bsky.social ! We wish you all the best!
Reposted by Akaash Kumar
🚨 Paper Alert 🚨
Our preprint describing Elongator as a selective microtubule polymerase that rewrites PTMs on microtubules is now published! Check it out: www.embopress.org/doi/full/10....
Our preprint describing Elongator as a selective microtubule polymerase that rewrites PTMs on microtubules is now published! Check it out: www.embopress.org/doi/full/10....
Elongator is a microtubule polymerase selective for polyglutamylated tubulin | The EMBO Journal
imageimageElongator, an established tRNA-modifying complex, can also stabilize microtubules
during asymmetric cell division. This study shows that Elongator subcomplexes achieve
this by binding to mic...
www.embopress.org
January 17, 2025 at 9:46 AM
🚨 Paper Alert 🚨
Our preprint describing Elongator as a selective microtubule polymerase that rewrites PTMs on microtubules is now published! Check it out: www.embopress.org/doi/full/10....
Our preprint describing Elongator as a selective microtubule polymerase that rewrites PTMs on microtubules is now published! Check it out: www.embopress.org/doi/full/10....
Reposted by Akaash Kumar
Congrats to @juliemiesch.bsky.social who was awarded a Postdoc Mobility grant by @snf-ch.bsky.social to support her research for two years. Her postdoc research will focus on the biophysical regulation of water buffering in the cell.
January 20, 2025 at 2:36 PM
Congrats to @juliemiesch.bsky.social who was awarded a Postdoc Mobility grant by @snf-ch.bsky.social to support her research for two years. Her postdoc research will focus on the biophysical regulation of water buffering in the cell.
Reposted by Akaash Kumar
Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
December 23, 2024 at 5:49 PM
Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Akaash Kumar
Congrats to @akaashkumar.bsky.social who has won the PhD Award from the Cambridge Centre for Physical Biology for his work developing a novel microscopy system with @deriverylab.bsky.social & @jamesdmanton.bsky.social in @cellbiol-mrclmb.bsky.social.
www.physbiol.cam.ac.uk/akaash-kumar
#LMBintheNews
www.physbiol.cam.ac.uk/akaash-kumar
#LMBintheNews
December 16, 2024 at 3:19 PM
Congrats to @akaashkumar.bsky.social who has won the PhD Award from the Cambridge Centre for Physical Biology for his work developing a novel microscopy system with @deriverylab.bsky.social & @jamesdmanton.bsky.social in @cellbiol-mrclmb.bsky.social.
www.physbiol.cam.ac.uk/akaash-kumar
#LMBintheNews
www.physbiol.cam.ac.uk/akaash-kumar
#LMBintheNews
Simultaneous multispectral imaging of seven organelles in live cells using our multispectral spinning disk confocal microscope! #FluorescenceFriday
You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
December 13, 2024 at 10:48 AM
Simultaneous multispectral imaging of seven organelles in live cells using our multispectral spinning disk confocal microscope! #FluorescenceFriday
You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...
Very happy that my work on developing multispectral live cell imaging technology has been recognised by the Cambridge Centre for Physical Biology! Thanks for the award!
Congrats to @akaashkumar.bsky.social , a postdoc in the lab, for winning the PhD Award from the Cambridge Centre for Physical Biology. His PhD research worked on the development of a multispectral imaging system to image up to 8 fluorophores similtaneously for tackling complex biological questions!
December 11, 2024 at 5:03 PM
Very happy that my work on developing multispectral live cell imaging technology has been recognised by the Cambridge Centre for Physical Biology! Thanks for the award!
Hi Mike, @dmikeando.bsky.social could I be added to this please?
December 1, 2024 at 9:36 PM
Hi Mike, @dmikeando.bsky.social could I be added to this please?
Reposted by Akaash Kumar
In June, @akaashkumar.bsky.social and @jamesdmanton.bsky.social had their Multispectral live-cell imaging paper released on to the BioRxiv www.biorxiv.org/content/10.1...
Multispectral live-cell imaging with uncompromised spatiotemporal resolution
Multispectral imaging is an established method to extend the number of colours usable in fluorescence imaging beyond the typical limit of three or four, but standard approaches are poorly suited to li...
www.biorxiv.org
November 22, 2024 at 1:23 PM
In June, @akaashkumar.bsky.social and @jamesdmanton.bsky.social had their Multispectral live-cell imaging paper released on to the BioRxiv www.biorxiv.org/content/10.1...
Reposted by Akaash Kumar
Then, in September @akaashkumar.bsky.social passed his PhD viva, becoming the latest doctor to emerge from the lab! He then won the RMS Early Career Award 2024 in October.
November 22, 2024 at 1:23 PM
Then, in September @akaashkumar.bsky.social passed his PhD viva, becoming the latest doctor to emerge from the lab! He then won the RMS Early Career Award 2024 in October.
Reposted by Akaash Kumar
Later in October, the LMB had their yearly annual symposium with Post-doc Lara Kruger winning the Steitz Prize for her talk and @akaashkumar.bsky.social winning the Perutz Prize for his PhD work!
November 22, 2024 at 1:23 PM
Later in October, the LMB had their yearly annual symposium with Post-doc Lara Kruger winning the Steitz Prize for her talk and @akaashkumar.bsky.social winning the Perutz Prize for his PhD work!
Some personal news: my wife and I are finally homeowners! We got the keys to our home in Cambridge today, very exciting times!
November 22, 2024 at 2:00 PM
Some personal news: my wife and I are finally homeowners! We got the keys to our home in Cambridge today, very exciting times!
Likewise! Took me a while aha hope you're well!
November 21, 2024 at 7:37 PM
Likewise! Took me a while aha hope you're well!
Would love to be added to this, for all things multispectral microscopy
November 21, 2024 at 7:05 PM
Would love to be added to this, for all things multispectral microscopy
Aha thank you very much!
November 21, 2024 at 6:13 PM
Aha thank you very much!
Would love to be added. Many updates on all things multispectral live cell imaging!
November 21, 2024 at 9:45 AM
Would love to be added. Many updates on all things multispectral live cell imaging!
Thanks for this, super useful for new joiners to bluesky (like me)! Could I be added to this group please?
November 20, 2024 at 9:49 PM
Thanks for this, super useful for new joiners to bluesky (like me)! Could I be added to this group please?