In future work, I am now using our multispectral technology to study membrane trafficking pathways in cytotoxic T cells and related immunotherapies, to decode how T cells are able to eliminate virally-infected/cancer cells and what happens in patients when these processes go wrong. Updates to come!

We demonstrated the ability to simultaneously image trafficking of five different endogenous receptor classes using de-novo binders from David Baker's lab, by adding the multispectral technology to a state-of-the-art single objective light sheet microscope, capturing data at 3 volumes per second.

The technology we developed is compatible with any camera-based microscope and by using polycistronic plasmids and live-cell dyes (e.g. @spirochrome.com) we can image multiple organelles in live cells simultaneously.

Today's light sheet video of actin dynamics in cytotoxic t cells... so much fun to come!

Simultaneous multispectral imaging of seven organelles in live cells using our multispectral spinning disk confocal microscope! #FluorescenceFriday
You can read more about this technology in our updated pre-print: www.biorxiv.org/content/10.1...

Hello Bluesky! I am an engineer/scientist currently working at the MRC LMB in Cambridge UK. During my PhD I developed new imaging technologies to shed light on the dynamics of living cells e.g. here is a movie of 6 organelles in live human cancer cells. Follow for more updates! #science #biology