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centriolelab.bsky.social
Paul & Virginie - Centriole Lab
@centriolelab.bsky.social
2K followers 2.7K following 20 posts
Virginie Hamel & Paul Guichard Lab at University of Geneva
#cryoEM/ET❄️ and #UExM ⚗️ #ExpansionMicroscopy #TeamTomo
Genève, Suisse 🇨🇭
https://mocel.unige.ch/research-groups/guichard-hamel/overv
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centriolelab.bsky.social
Paul & Virginie - Centriole Lab @centriolelab.bsky.social · Aug 28
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Reposted by Paul & Virginie - Centriole Lab
bioarm.bsky.social
This is a glimpse of the cytoskeletal structures we found doing U-ExM in more than 200 species, now in Cell🤩: shorturl.at/6oHvi
Amazing collaboration between @centriolelab.bsky.social , @dudinlab.bsky.social and @gautamdey.bsky.social labs.
I believe last one is a 🕷️
#FluorescenceFriday
#Microscopy
November 7, 2025 at 1:35 PM
Reposted by Paul & Virginie - Centriole Lab
tbharat-lab.bsky.social
An Asgard archaeon with internal membrane compartments

Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!

www.biorxiv.org/content/10.1...
November 7, 2025 at 10:44 AM
Reposted by Paul & Virginie - Centriole Lab
jcellsci.bsky.social
In their Review, Olivier Mercey, @marinebrunet.bsky.social, Paul Guichard & Virginie Hamel @centriolelab.bsky.social highlight advances in microscopy that have significantly deepened our understanding of centriolar & ciliary structure & function.
journals.biologists.com/jcs/article/...
#OpenAccess
Advances in high-resolution imaging of centrioles and cilia
Summary: A review of the progressive advances in light and electron microscopy that have revealed unprecedented structural and molecular insights into centrioles and cilia, and reshaped our view of th...
journals.biologists.com
November 5, 2025 at 3:37 PM
Reposted by Paul & Virginie - Centriole Lab
jcellsci.bsky.social
Our #JCSciliaSI is complete

Explore our ToC: journals.biologists.com/jcs/issue/13...

Our cover image shows tracheal epithelial cells from an air-liquid interface culture imaged using U-ExM from Oliver Mercey and Marine Brunet @centriolelab.bsky.social.
journals.biologists.com/jcs/article/...
JCS Cover: Human tracheal epithelial cells (hTECs) from an air–liquid interface culture imaged using ultrastructural expansion microscopy (U-ExM). Cells are labelled for tubulin (magenta), mCLING (cyan) and DNA (blue, DAPI). Image from Olivier Mercey and Marine Brunet in the Guichard-Hamel lab. See article by O. Mercey et al. (jcs264260).
November 4, 2025 at 1:36 PM
Reposted by Paul & Virginie - Centriole Lab
embl.org
(1/2) A new study from EMBL researchers and their collaborators provides unprecedented insights into the cellular architecture of over 200 species of plankton – tiny but ecologically critical marine organisms – using ultrastructure expansion microscopy.

Learn more: www.cell.com/cell/fulltex...
November 4, 2025 at 8:30 AM
Reposted by Paul & Virginie - Centriole Lab
floravincent.bsky.social
Exciting day for the lab: our 1rst paper is officially out in @currentbiology.bsky.social 🥳 Wonderful collaboration wt @gautamdey.bsky.social showing how Cryo-ExM achieves consistent immunostaining in diverse diatoms, from the lab and the natural environment 1/n
#ProtistsOnSky
tinyurl.com/2zxaund7
October 31, 2025 at 3:27 PM
centriolelab.bsky.social
In French - Virginie on Swiss radio! 🎙️ She talks about our latest collab with @dudinlab.bsky.social and @gautamdey.bsky.social #UExM #ProtistsOnSky
November 3, 2025 at 5:13 PM
Reposted by Paul & Virginie - Centriole Lab
biology-unige.bsky.social
📻 Interview de Virginie Hamel du @centriolelab.bsky.social pour CQFD de la #RTS: Une méthode ouvre lʹaccès à lʹarchitecture interne du #plancton #microscopie #expansion www.rts.ch/audio-podcas... @mocel.bsky.social @sciencesunige.bsky.social @dudinlab.bsky.social
Une méthode ouvre lʹaccès à lʹarchitecture interne du plancton | RTS
Une étude majeure menée par l’Université de Genève et le Laboratoire européen de biologie moléculaire, publiée dans la revue Cell, révèle l’architecture interne de plus de deux cents espèces de planct...
www.rts.ch
November 3, 2025 at 4:16 PM
Reposted by Paul & Virginie - Centriole Lab
marinebrunet.bsky.social
Exciting news: the new review from @centriolelab.bsky.social is out in the amazing #JCSciliaSI! 🎉
I had the pleasure of working alongside Olivier Mercey, Paul Guichard and Virginie Hamel on this one.
Cherry on top: our #UExM image was selected for the cover of the issue! 🤩
Advances in high-resolution imaging of centrioles and cilia
Summary: A review of the progressive advances in light and electron microscopy that have revealed unprecedented structural and molecular insights into centrioles and cilia, and reshaped our view of th...
journals.biologists.com
November 3, 2025 at 2:47 PM
Reposted by Paul & Virginie - Centriole Lab
biology-unige.bsky.social
‪
Congratulations @centriolelab.bsky.social @dudinlab.bsky.social and @gautamdey.bsky.social for your publication in @cp-cell.bsky.social: Charting the landscape of #cytoskeletal diversity in #microbial eukaryotes #Expansion #Microscopy @sciencesunige.bsky.social
www.unige.ch/medias/en/20...
November 3, 2025 at 1:34 PM
Reposted by Paul & Virginie - Centriole Lab
manuelthery.bsky.social
nine fold symmetry
those-who-know know what i mean
November 1, 2025 at 12:53 PM
Reposted by Paul & Virginie - Centriole Lab
dudinlab.bsky.social
🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky

www.cell.com/cell/fulltex...
October 31, 2025 at 2:42 PM
Reposted by Paul & Virginie - Centriole Lab
chlandalab.bsky.social
Mysteries of trafficking of the vRNP segmented genome and assembly of influenza A virus revealed by in situ cryo-ET!
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.
October 24, 2025 at 8:11 AM
Reposted by Paul & Virginie - Centriole Lab
qiangguo.bsky.social
We usually learn that centrioles/basal bodies are made of microtubule triplets. But our work on Plasmodium shows its basal body is built from singlets, which then mature into the doublets of a functional cilium. #TEAMTOMO www.nature.com/articles/s41...
A basal body microtubule singlet-to-doublet transition in Plasmodium male gametogenesis - Nature Communications
Yang et al. use cryo-ET and resolve a unique native architecture of microtubule singlet-to-doublet transition in the basal body of Plasmodium. They reveal the key role of δTubulin and ε-Tubulin in thi...
www.nature.com
October 16, 2025 at 8:28 AM
Reposted by Paul & Virginie - Centriole Lab
nadjahuempf.bsky.social
It is out! 🦚 I recorded hundreds of #ExM 🔬images of cytokinetic bridges and averaged them into 6 stages. How? With help from our fantastic collaborators @zacsimile.bsky.social and @jonasries.bsky.social in Vienna 😇. Check out the full atlas here: doi.org/10.5281/zenodo.17232370
Sketches of six stages of cytokinetic development of the intercellular bridge are illustrated with exmaple U-ExM fluorescence microscopy images. Septin2-GFP shown in green. Tubulin shown in magenta. A shortened workflow illustrates the image alignment and averaging process to generate average 2D reconstructions of cytokinetic stages.
September 30, 2025 at 4:25 PM
Reposted by Paul & Virginie - Centriole Lab
robklose.bsky.social
We are recuiting two new Associate Professors here in Oxford Biochemistry. Come join us! Reach out to me if you have any questions. Please repost! tinyurl.com/mr3m7bd3
October 17, 2025 at 2:56 PM
Reposted by Paul & Virginie - Centriole Lab
spirochrome.com
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.

Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...

Image credits: Olivier Mercey, UNIGE
U-ExM expanded HeLa cell stained with HAK-actin (orange) and DNA (blue).
September 19, 2025 at 9:45 AM
Reposted by Paul & Virginie - Centriole Lab
manuelthery.bsky.social
We hire!
The CytoMorpho Lab is looking for an engineer with a background in cell and molecular biology to join our team in Paris. This is a 18-month contract position, but we're open to exploring long-term opportunities.
More details here:
cytomorpholab.com/index.php/jo...
October 14, 2025 at 1:50 PM
Reposted by Paul & Virginie - Centriole Lab
joedobbs.bsky.social
Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️

www.biorxiv.org/content/10.1...
#teamtomo
Figure 1 from the preprint
October 15, 2025 at 6:26 AM
centriolelab.bsky.social
The A–C linker structure!!! 🤩 Beautiful work. Even if you’re not as centriole-obsessed as we are, you’ll appreciate the elegance and complexity. Amazing to see U-ExM proteins in human centrioles fitting right into these densities. Congrats to all!
mike-wieczorek.bsky.social Wieczorek Lab @mike-wieczorek.bsky.social · Oct 10
Huge congratulations to Wieczorek Lab PhD student Bin Cai and now-PI Jingwei Xu @xujwet.bsky.social on figuring out the surprisingly complex structure of the A-C linker crosslinking microtubule triplets in Tetrahymena basal body centrioles! www.science.org/doi/10.1126/...
Structure and assembly of the A-C linker connecting microtubule triplets in centrioles
Multiscale cryo-EM of centrioles reveals the structure and polymorphism of the A-C linker connecting microtubule triplets.
www.science.org
October 13, 2025 at 10:03 AM
Reposted by Paul & Virginie - Centriole Lab
plosbiology.org
The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6
A Trypanosoma brucei cell visualized by confocal microscopy after ultrastructure expansion. The cell was immunolabelled for the BILBO1 protein (yellow) and stained with fluorescent NHS-ester (grey). The flagellar pocket is highlighted in pink. Credit: Marie Zelená
October 10, 2025 at 4:14 PM
Reposted by Paul & Virginie - Centriole Lab
alishaib.bsky.social
#GEF25 Concluding Plenary Lecture with the wonderful Virginie Hamel.
September 30, 2025 at 10:29 AM
Reposted by Paul & Virginie - Centriole Lab
christlet.bsky.social
The ExM gang @jvermot.bsky.social @mahjoublab.bsky.social @marinelap.bsky.social @centriolelab.bsky.social @simonemortal.bsky.social et al #GEF25
September 29, 2025 at 8:04 PM
Reposted by Paul & Virginie - Centriole Lab
borissieber.bsky.social
Mammalian cells have KSR, budding yeast has Ste5… and fission yeast has Sms1 as the MAPK scaffold for sexual reproduction!

Very excited to share my postdoc work where we discover that the hemi-arrestin Sms1 binds all components of the MAPK cascade, including ERK-like Spk1

doi.org/10.1101/2025...
Schematic of MAPK signalling in fission yeast, budding yeast and Metazoa. No MAPK scaffold was known for ERK-like MAPK in fission yeast, as opposed to budding yeast Ste5 and metazoan KSR1/2.
September 25, 2025 at 4:27 PM