Tomas Janovic
@janovictom.bsky.social
Postdoc at Masaryk University, Brno, Czechia | Advanced microscopy, Single-molecule biophysics, Telomeres, Genome maintenance.
Reposted by Tomas Janovic
New Print Alert! A fun collab with Kathy Meek at MSU. Led by talented bioinformatician Giovanni Pascarella we demonstrate that Ku70/80 binds intronic antisense Alu elements to inhibit cryptic splice sites. For details see the thread below! 1/n
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Ku limits aberrant mRNA splicing promoted by intronic antisense Alu elements
Alu elements are short repeats that occupy approximately 10% of the human genome. Saturation of primate genomes with Alu sequences occurred at the prosimian/new-world monkey evolutionary juncture. Alu...
www.biorxiv.org
November 21, 2025 at 3:52 AM
New Print Alert! A fun collab with Kathy Meek at MSU. Led by talented bioinformatician Giovanni Pascarella we demonstrate that Ku70/80 binds intronic antisense Alu elements to inhibit cryptic splice sites. For details see the thread below! 1/n
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Tomas Janovic
IT'S HAPPENING! 💥 I'm psyched to launch the collaboration between @qedscience.bsky.social & @openrxiv.bsky.social @biorxivpreprint.bsky.social! Preprint + q.e.d = your science is out there, and anyone can appreciate it. Let's care about making discoveries, and not on “getting published” (1/3) 👇
November 6, 2025 at 2:49 PM
IT'S HAPPENING! 💥 I'm psyched to launch the collaboration between @qedscience.bsky.social & @openrxiv.bsky.social @biorxivpreprint.bsky.social! Preprint + q.e.d = your science is out there, and anyone can appreciate it. Let's care about making discoveries, and not on “getting published” (1/3) 👇
Reposted by Tomas Janovic
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Human RPA is an essential telomerase processivity factor for maintaining telomeres
Telomerase counteracts telomere shortening by repeatedly adding DNA repeats to chromosome ends. We identified the replication protein A (RPA) heterotrimer as a telomerase processivity factor critical ...
www.science.org
October 30, 2025 at 10:07 PM
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Reposted by Tomas Janovic
1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from?
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Antiviral reverse transcriptases reveal the evolutionary origin of telomerase
Defense-associated reverse transcriptases (DRTs) employ diverse and distinctive mechanisms of cDNA synthesis to protect bacteria against viral infection. However, much of DRT family diversity remains ...
www.biorxiv.org
October 17, 2025 at 5:22 PM
1/10 Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from?
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria.
www.biorxiv.org/content/10.1...
Reposted by Tomas Janovic
Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11
Read the thread below for a summary of our findings 🧵1/11
September 17, 2025 at 2:55 PM
Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11
Read the thread below for a summary of our findings 🧵1/11
🚨 Excited to share my main postdoc work from the @jenscs83.bsky.social lab, now out in Cell Reports! 👉 www.sciencedirect.com/science/arti... We dissect how shelterin proteins organize at telomeres in human cancer cells—revealing distinct subcomplexes that maintain chromosome ends. 🧵
TRF1 and TRF2 form distinct shelterin subcomplexes at telomeres
The shelterin complex protects chromosome ends from aberrant DNA repair and regulates telomerase access to telomeres. Shelterin is composed of six pro…
www.sciencedirect.com
August 25, 2025 at 1:27 PM
🚨 Excited to share my main postdoc work from the @jenscs83.bsky.social lab, now out in Cell Reports! 👉 www.sciencedirect.com/science/arti... We dissect how shelterin proteins organize at telomeres in human cancer cells—revealing distinct subcomplexes that maintain chromosome ends. 🧵
Reposted by Tomas Janovic
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 3, 2025 at 7:45 PM
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
Reposted by Tomas Janovic
Great new DNA/Q-Paint method published by our @ucl colleague Sabrina Simoncelli - lucky to have been part of it! Check out the Ecadherin and collagenIV imaging in the fly retina at up to 15micron depth www.nature.com/articles/s41...
Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment - Nature Communications
Zaza and colleagues demonstrate that DNA-PAINT on a spinning disk confocal microscope with optical photon reassignment enables high-resolution imaging across large fields and imaging depths, resolving...
www.nature.com
May 29, 2025 at 7:10 PM
Great new DNA/Q-Paint method published by our @ucl colleague Sabrina Simoncelli - lucky to have been part of it! Check out the Ecadherin and collagenIV imaging in the fly retina at up to 15micron depth www.nature.com/articles/s41...
Reposted by Tomas Janovic
Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com
www.nature.com/articles/s41...
@spirochrome.com
A palette of bridged bicycle-strengthened fluorophores - Nature Methods
A suite of bridged rhodamine dyes (BriDyes) offers excellent brightness, solubility, photostability, and tunable cell permeability along with resistance to photoblueing, making them exceptional all-pu...
www.nature.com
May 20, 2025 at 2:11 PM
Here come our new palette of fluorescent dyes. The BD dyes endeaves to balance glowy brightness, robust photostability, and biocompatibility. Let's boost 4D dynamic super-resolution imaging!!!
www.nature.com/articles/s41...
@spirochrome.com
www.nature.com/articles/s41...
@spirochrome.com
Reposted by Tomas Janovic
The ERC welcomes the offer of substantial additional budget from @ec.europa.eu for the development of a new ERC funding instrument offering larger, longer-term grants, as announced by President Ursula Von der Leyen at the Sorbonne this morning.
👉 europa.eu/!NTYTTV
👉 europa.eu/!NTYTTV
Choose Europe for Science: ERC welcomes new budget for 'super grants'
The Scientific Council of the European Research Council welcomes the offer of substantial additional budget from the European Commission for the development of a new ERC funding instrument offering la...
europa.eu
May 5, 2025 at 11:41 AM
The ERC welcomes the offer of substantial additional budget from @ec.europa.eu for the development of a new ERC funding instrument offering larger, longer-term grants, as announced by President Ursula Von der Leyen at the Sorbonne this morning.
👉 europa.eu/!NTYTTV
👉 europa.eu/!NTYTTV
Reposted by Tomas Janovic
A great end to another Telomeres CSH meeting! It was nice to see and discuss science with many colleagues. With @cijilim.bsky.social @jenscs83.bsky.social, Stone, Nandakumar, Zappulla labs at banquet
May 4, 2025 at 2:10 PM
A great end to another Telomeres CSH meeting! It was nice to see and discuss science with many colleagues. With @cijilim.bsky.social @jenscs83.bsky.social, Stone, Nandakumar, Zappulla labs at banquet
Reposted by Tomas Janovic
🚨🔬💗Whether investigating cell organelles or mapping proteins, together with Victor Puelles's lab we lay a roadmap for selecting optimal #ExM and #SuperResolution #microscopy combinations. Daria Aristova and Dominik Kylies review with amazing co-authors
pubs.aip.org/aip/apr/arti...
pubs.aip.org/aip/apr/arti...
April 25, 2025 at 5:21 PM
🚨🔬💗Whether investigating cell organelles or mapping proteins, together with Victor Puelles's lab we lay a roadmap for selecting optimal #ExM and #SuperResolution #microscopy combinations. Daria Aristova and Dominik Kylies review with amazing co-authors
pubs.aip.org/aip/apr/arti...
pubs.aip.org/aip/apr/arti...
Reposted by Tomas Janovic
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
April 11, 2025 at 8:35 AM
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
Reposted by Tomas Janovic
Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social!
Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩
www.sciencedirect.com/science/arti...
Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩
www.sciencedirect.com/science/arti...
March 24, 2025 at 3:29 PM
Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social!
Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩
www.sciencedirect.com/science/arti...
Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩
www.sciencedirect.com/science/arti...
Reposted by Tomas Janovic
1/Out today in @naturecomms.bsky.social,
“A CPC-shelterin-BTR axis regulates mitotic telomere deprotection”.
Here we identify the mechanism that unwinds telomere-loops (t-loops) during mitotic arrest to activate the DNA damage response and signal mitotic stress.
www.nature.com/articles/s41....
“A CPC-shelterin-BTR axis regulates mitotic telomere deprotection”.
Here we identify the mechanism that unwinds telomere-loops (t-loops) during mitotic arrest to activate the DNA damage response and signal mitotic stress.
www.nature.com/articles/s41....
A CPC-shelterin-BTR axis regulates mitotic telomere deprotection - Nature Communications
Here the authors reveal how telomeres signal mitotic stress. A key protein network alters their structure exposing telomere ends to signal mitotic stress, ultimately triggering a controlled DNA damage...
www.nature.com
March 17, 2025 at 10:32 AM
1/Out today in @naturecomms.bsky.social,
“A CPC-shelterin-BTR axis regulates mitotic telomere deprotection”.
Here we identify the mechanism that unwinds telomere-loops (t-loops) during mitotic arrest to activate the DNA damage response and signal mitotic stress.
www.nature.com/articles/s41....
“A CPC-shelterin-BTR axis regulates mitotic telomere deprotection”.
Here we identify the mechanism that unwinds telomere-loops (t-loops) during mitotic arrest to activate the DNA damage response and signal mitotic stress.
www.nature.com/articles/s41....
Reposted by Tomas Janovic
#Hiring! The postdoctoral position in my lab at Pharm Chem KU is open!! This is an NIH-funded position to work on cytochrome P450 metabolism using a combination of mass spectrometry proteomics and live-cell imaging.
employment.ku.edu/staff/29681BR
employment.ku.edu/staff/29681BR
Postdoctoral Researcher - University of Kansas - Job Details
Job Details: A full-time Postdoctoral Research Associate position is available at the University of Kansas Department of Ph
employment.ku.edu
January 23, 2025 at 12:47 PM
#Hiring! The postdoctoral position in my lab at Pharm Chem KU is open!! This is an NIH-funded position to work on cytochrome P450 metabolism using a combination of mass spectrometry proteomics and live-cell imaging.
employment.ku.edu/staff/29681BR
employment.ku.edu/staff/29681BR
Reposted by Tomas Janovic
Super excited to share our most recent pre-print, lead by K99 fellow @heyzajr.bsky.social, who will be starting his own lab at Wayne State University in February!
The question is how are DNA breaks held together during HR?? 1/n
www.biorxiv.org/content/10.1...
The question is how are DNA breaks held together during HR?? 1/n
www.biorxiv.org/content/10.1...
The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain
DNA double strand breaks (DSBs) are widely considered the most cytotoxic DNA lesions occurring in cells because they physically disrupt the connectivity of the DNA double helix. Homologous recombinati...
www.biorxiv.org
January 13, 2025 at 2:26 PM
Super excited to share our most recent pre-print, lead by K99 fellow @heyzajr.bsky.social, who will be starting his own lab at Wayne State University in February!
The question is how are DNA breaks held together during HR?? 1/n
www.biorxiv.org/content/10.1...
The question is how are DNA breaks held together during HR?? 1/n
www.biorxiv.org/content/10.1...
Reposted by Tomas Janovic
1/Delighted to announce the newest paper from our lab, “Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage”, is out today in @naturecellbiology.bsky.social, www.nature.com/articles/s41....
Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage - Nature Cell Biology
Szmyd et al. show that DNA repair pathways impact whether cells with DNA lesions arrest in mitosis. The formation of homologous recombination-driven double Holliday junctions elicits mitotic cell deat...
www.nature.com
January 13, 2025 at 10:12 AM
1/Delighted to announce the newest paper from our lab, “Homologous recombination promotes non-immunogenic mitotic cell death upon DNA damage”, is out today in @naturecellbiology.bsky.social, www.nature.com/articles/s41....
Reposted by Tomas Janovic
We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
January 7, 2025 at 8:45 AM
We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
1/8 Super excited to share my first-author preprint in @jenscs83.bsky.social lab!
We measured the shelterin complex stoichiometry and dynamics at telomeres in living cells. Our results re-envisions the architecture of telomeres in human cancer cells.
www.biorxiv.org/content/10.1...
We measured the shelterin complex stoichiometry and dynamics at telomeres in living cells. Our results re-envisions the architecture of telomeres in human cancer cells.
www.biorxiv.org/content/10.1...
TRF1 and TRF2 form distinct shelterin subcomplexes at telomeres
The shelterin complex protects chromosome ends from the DNA damage repair machinery and regulates telomerase access to telomeres. Shelterin is composed of six proteins (TRF1, TRF2, TIN2, TPP1, POT1 an...
www.biorxiv.org
December 24, 2024 at 11:41 AM
1/8 Super excited to share my first-author preprint in @jenscs83.bsky.social lab!
We measured the shelterin complex stoichiometry and dynamics at telomeres in living cells. Our results re-envisions the architecture of telomeres in human cancer cells.
www.biorxiv.org/content/10.1...
We measured the shelterin complex stoichiometry and dynamics at telomeres in living cells. Our results re-envisions the architecture of telomeres in human cancer cells.
www.biorxiv.org/content/10.1...
Reposted by Tomas Janovic
Our paper of today is the result of the creative hard work of first authors Richard Janissen and @romanbarth.bsky.social in our #CDlab, in collaboration with Iain Davidson and Jan-Michael Peters of the IMP Vienna BioCenter.
Enjoy reading our paper here: www.science.org/doi/10.1126/...
10/10
Enjoy reading our paper here: www.science.org/doi/10.1126/...
10/10
All eukaryotic SMC proteins induce a twist of −0.6 at each DNA loop extrusion step
Measuring single DNA loop extrusion steps of individual eukaryotic SMCs revealed that ATP binding induces a DNA twist of −0.6.
www.science.org
December 13, 2024 at 7:25 PM
Our paper of today is the result of the creative hard work of first authors Richard Janissen and @romanbarth.bsky.social in our #CDlab, in collaboration with Iain Davidson and Jan-Michael Peters of the IMP Vienna BioCenter.
Enjoy reading our paper here: www.science.org/doi/10.1126/...
10/10
Enjoy reading our paper here: www.science.org/doi/10.1126/...
10/10
Reposted by Tomas Janovic
🔺🔺🔺RED TRIANGLE ALERT 🔺🔺🔺
Ever wonder how #TADs compare across the tree of life?Look no further & read our Review!!!
Find out what genes & 3D chromatin can & can't do in Bacteria! Archeae! Yeast! Plants! Animals!
SMCs & RNA-Pol are the only thing they have in common
www.nature.com/articles/s41...
Ever wonder how #TADs compare across the tree of life?Look no further & read our Review!!!
Find out what genes & 3D chromatin can & can't do in Bacteria! Archeae! Yeast! Plants! Animals!
SMCs & RNA-Pol are the only thing they have in common
www.nature.com/articles/s41...
Evolution and function of chromatin domains across the tree of life - Nature Structural & Molecular Biology
Szalay et al. discuss cross-kingdom similarities and differences in 3D chromatin folding in relation to gene regulation, including in bacteria, archaea, mammals and plants. This comparison reveals cer...
www.nature.com
November 28, 2024 at 12:48 PM
🔺🔺🔺RED TRIANGLE ALERT 🔺🔺🔺
Ever wonder how #TADs compare across the tree of life?Look no further & read our Review!!!
Find out what genes & 3D chromatin can & can't do in Bacteria! Archeae! Yeast! Plants! Animals!
SMCs & RNA-Pol are the only thing they have in common
www.nature.com/articles/s41...
Ever wonder how #TADs compare across the tree of life?Look no further & read our Review!!!
Find out what genes & 3D chromatin can & can't do in Bacteria! Archeae! Yeast! Plants! Animals!
SMCs & RNA-Pol are the only thing they have in common
www.nature.com/articles/s41...
Reposted by Tomas Janovic
“In this Review, we discuss #telomere protection, maintenance and damage. We highlight the differences between #human and #mouse telomere biology that may contribute to discrepancies between human #diseases and mouse models.” #telomerase #aging
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Telomere function and regulation from mouse models to human ageing and disease - Nature Reviews Molecular Cell Biology
Telomere dysfunction, usually owing to shortening, activates cellular senescence and can contribute to age-associated diseases and cancer. Mouse models are crucial for telomere research, but human and...
www.nature.com
November 30, 2024 at 6:02 AM
“In this Review, we discuss #telomere protection, maintenance and damage. We highlight the differences between #human and #mouse telomere biology that may contribute to discrepancies between human #diseases and mouse models.” #telomerase #aging
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tomas Janovic
Super excited to share our latest paper led by graduate student Mariia Mikhova! She used single molecule live cell imaging to dissect the kinetics of DNA break repair by non homologous end joining. We estimate the NHEJ pathway can repair up to 1000 breaks per minute!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells - Nature Communications
Non-homologous end joining repairs most double stranded DNA breaks in human cells. Here, the authors apply a single-molecule imaging approach visualizing the recruitment of DNA repair proteins to DNA ...
www.nature.com
November 23, 2024 at 1:14 AM
Super excited to share our latest paper led by graduate student Mariia Mikhova! She used single molecule live cell imaging to dissect the kinetics of DNA break repair by non homologous end joining. We estimate the NHEJ pathway can repair up to 1000 breaks per minute!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tomas Janovic
First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5
November 13, 2024 at 8:23 AM
First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5