Johannes Hohlbein
@hohlbeinlab.bsky.social
Single-molecule stuff, 🔬, 🦠 , 🧬, ☕, Assoc. Prof. @WUR (Laboratory of Biophysics, Wageningen), he/him, 🇩🇪->🇨🇿->🇩🇪->🇬🇧->🇳🇱, Pubs: https://scholar.google.nl/citations?hl=en&user=WZZAydwAAAAJ&view_op=list_works&sortby=pubdate
Pinned
First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5
4:35pm is an excellent time to teach the ins and outs of the harmonic oscillator. Followed by a computational tutorial until 6:55pm. :) #IteachPhysics
November 6, 2025 at 3:13 PM
4:35pm is an excellent time to teach the ins and outs of the harmonic oscillator. Followed by a computational tutorial until 6:55pm. :) #IteachPhysics
Reposted by Johannes Hohlbein
ICYMI – Check out our latest #preprint list on FocalPlane, highlighting the latest tools and techniques in microscopy.
focalplane.biologists.com/2025/10/22/m...
focalplane.biologists.com/2025/10/22/m...
Microscopy preprints – new tools and techniques in imaging - FocalPlane
Microscopy preprints – new tools and techniques in imaging - News
focalplane.biologists.com
October 27, 2025 at 4:27 PM
ICYMI – Check out our latest #preprint list on FocalPlane, highlighting the latest tools and techniques in microscopy.
focalplane.biologists.com/2025/10/22/m...
focalplane.biologists.com/2025/10/22/m...
Reposted by Johannes Hohlbein
🦠🔬🤖🧑💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training
📜 www.biorxiv.org/content/10.1...
📜 www.biorxiv.org/content/10.1...
October 22, 2025 at 11:10 AM
🦠🔬🤖🧑💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training
📜 www.biorxiv.org/content/10.1...
📜 www.biorxiv.org/content/10.1...
The good things are sometimes very close. Keep your eyes open.
October 4, 2025 at 2:15 PM
The good things are sometimes very close. Keep your eyes open.
Celebrated 2222 citations on Twitter. 3333 on BlueSky. 4444 on ???
October 1, 2025 at 5:43 PM
Celebrated 2222 citations on Twitter. 3333 on BlueSky. 4444 on ???
Reposted by Johannes Hohlbein
I have an online Eisenhower To-Do list where I copy & paste all completed tasks to the bottom of the list. Eisenhower: 1) important and urgent (Do!), 2) important but not urgent (Schedule!), 3) not important but urgent (Delegate!), 4) not important not urgent (Ignore!)
October 1, 2025 at 11:56 AM
I have an online Eisenhower To-Do list where I copy & paste all completed tasks to the bottom of the list. Eisenhower: 1) important and urgent (Do!), 2) important but not urgent (Schedule!), 3) not important but urgent (Delegate!), 4) not important not urgent (Ignore!)
Reposted by Johannes Hohlbein
Our 6-hands review/perspective: "Hacking 3D printers as laboratory robots" is now on ChemRxiv.
In there, we show how many robots you can make by simply modifying a 3D printer. #chemsky 1/n
with @sandersays.bsky.social and @supersciencegrl.co.uk
chemrxiv.org/engage/chemr...
In there, we show how many robots you can make by simply modifying a 3D printer. #chemsky 1/n
with @sandersays.bsky.social and @supersciencegrl.co.uk
chemrxiv.org/engage/chemr...
September 30, 2025 at 2:07 PM
Our 6-hands review/perspective: "Hacking 3D printers as laboratory robots" is now on ChemRxiv.
In there, we show how many robots you can make by simply modifying a 3D printer. #chemsky 1/n
with @sandersays.bsky.social and @supersciencegrl.co.uk
chemrxiv.org/engage/chemr...
In there, we show how many robots you can make by simply modifying a 3D printer. #chemsky 1/n
with @sandersays.bsky.social and @supersciencegrl.co.uk
chemrxiv.org/engage/chemr...
Reposted by Johannes Hohlbein
Picard management tip: Some of your decisions are going to be mistakes. You have to make them anyway.
September 28, 2025 at 3:04 PM
Picard management tip: Some of your decisions are going to be mistakes. You have to make them anyway.
Picard is always right, fellow scientists.
Picard management tip: Even without game-changing results, experimentation is time well spent.
September 26, 2025 at 8:54 PM
Picard is always right, fellow scientists.
Friend got me some Corsican chestnut flour. Turned out rather well. #sourdough
September 21, 2025 at 5:45 PM
Friend got me some Corsican chestnut flour. Turned out rather well. #sourdough
Shower thought: instead of paying $$$ for article processing charges and open access, why don’t I/we preprint and pay two reviewers $ to improve the manuscript?
September 15, 2025 at 7:32 PM
Shower thought: instead of paying $$$ for article processing charges and open access, why don’t I/we preprint and pay two reviewers $ to improve the manuscript?
Reposted by Johannes Hohlbein
An diejenigen unter euch, die selbst in der Wissenschaft tätig sind:
'Dinge, von denen ich mir wünsche, ich hätte sie früher gewusst' - was wäre eure Antwort gewesen?
'Dinge, von denen ich mir wünsche, ich hätte sie früher gewusst' - was wäre eure Antwort gewesen?
September 13, 2025 at 6:19 AM
An diejenigen unter euch, die selbst in der Wissenschaft tätig sind:
'Dinge, von denen ich mir wünsche, ich hätte sie früher gewusst' - was wäre eure Antwort gewesen?
'Dinge, von denen ich mir wünsche, ich hätte sie früher gewusst' - was wäre eure Antwort gewesen?
Reposted by Johannes Hohlbein
Please share: The application for Les Houches is open (until Oct 31)
fluorescenceleshouches.wordpress.com
*This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!*
PS: no fees this year, you just need to get there, that's all!
fluorescenceleshouches.wordpress.com
*This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!*
PS: no fees this year, you just need to get there, that's all!
French-German WE-Heraeus-Seminar "Fluorescence markers for advanced microscopy : From photophysics to biology - 2026"
March 15-20 2026, Les Houches, France
fluorescenceleshouches.wordpress.com
September 12, 2025 at 9:03 AM
Please share: The application for Les Houches is open (until Oct 31)
fluorescenceleshouches.wordpress.com
*This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!*
PS: no fees this year, you just need to get there, that's all!
fluorescenceleshouches.wordpress.com
*This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!*
PS: no fees this year, you just need to get there, that's all!
Reposted by Johannes Hohlbein
After being frustrated for several years with struggling to build electronics-intensive microfluidic setup, that finally led to development of the significant part of control electronics by myself, I realized it would be nice to develop some kind of #microfluidics hardware development kit, 🧵
September 9, 2025 at 12:26 PM
After being frustrated for several years with struggling to build electronics-intensive microfluidic setup, that finally led to development of the significant part of control electronics by myself, I realized it would be nice to develop some kind of #microfluidics hardware development kit, 🧵
Puzzles me why one has to scroll to the bottom of the webpage to resolve a DOI at www.doi.org.
September 9, 2025 at 7:56 AM
Puzzles me why one has to scroll to the bottom of the webpage to resolve a DOI at www.doi.org.
Reposted by Johannes Hohlbein
Dear scientific community,
I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.
my.corehr.com/pls/uoxrecru...
my.corehr.com/pls/uoxrecru...
I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.
my.corehr.com/pls/uoxrecru...
my.corehr.com/pls/uoxrecru...
September 8, 2025 at 3:16 PM
Dear scientific community,
I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.
my.corehr.com/pls/uoxrecru...
my.corehr.com/pls/uoxrecru...
I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.
my.corehr.com/pls/uoxrecru...
my.corehr.com/pls/uoxrecru...
Reposted by Johannes Hohlbein
Another academic year, another NWO Physics of Life newsletter!
Check it out 👇
nwobiophysics.nl/newsletters
> Interview with former chair Kristina Ganzinger (AMOLF) and our new chair Kristin Grußmayer @tudelfttnw.bsky.social
> Biosketch by Lisa Tran @utrechtuniversity.bsky.social
> and more..
Check it out 👇
nwobiophysics.nl/newsletters
> Interview with former chair Kristina Ganzinger (AMOLF) and our new chair Kristin Grußmayer @tudelfttnw.bsky.social
> Biosketch by Lisa Tran @utrechtuniversity.bsky.social
> and more..
September 4, 2025 at 2:55 PM
Another academic year, another NWO Physics of Life newsletter!
Check it out 👇
nwobiophysics.nl/newsletters
> Interview with former chair Kristina Ganzinger (AMOLF) and our new chair Kristin Grußmayer @tudelfttnw.bsky.social
> Biosketch by Lisa Tran @utrechtuniversity.bsky.social
> and more..
Check it out 👇
nwobiophysics.nl/newsletters
> Interview with former chair Kristina Ganzinger (AMOLF) and our new chair Kristin Grußmayer @tudelfttnw.bsky.social
> Biosketch by Lisa Tran @utrechtuniversity.bsky.social
> and more..
Going to the UK by direct train from Rotterdam. 2 min in total for checking luggage, EU, and UK border control. Nice.
September 3, 2025 at 11:21 AM
Going to the UK by direct train from Rotterdam. 2 min in total for checking luggage, EU, and UK border control. Nice.
Reposted by Johannes Hohlbein
Excited to share my postdoc work from the Eggeling lab in Jena (@leibnizipht.bsky.social, #FSUJena, #KTHuniversity) on bringing smart microscopy to super-resolution MINFLUX: event-triggered MINFLUX microscopy.
September 2, 2025 at 8:59 AM
Excited to share my postdoc work from the Eggeling lab in Jena (@leibnizipht.bsky.social, #FSUJena, #KTHuniversity) on bringing smart microscopy to super-resolution MINFLUX: event-triggered MINFLUX microscopy.
"Offer to order reprints (professional hard copy prints)" of an article in an online-only journal? Me not understand.
August 31, 2025 at 6:33 PM
"Offer to order reprints (professional hard copy prints)" of an article in an online-only journal? Me not understand.
Reposted by Johannes Hohlbein
The Dutch Physics Council just circulated their biannual newsletter. Not yet registered? Sign up here: dutchphysicscouncil.nl/news/#newsle...
Amongst others we share the interview published in het Nederlands Tijdschrift voor #Natuurkunde with our chair Esther Alarcón Lladó @3dpv-group.bsky.social
Amongst others we share the interview published in het Nederlands Tijdschrift voor #Natuurkunde with our chair Esther Alarcón Lladó @3dpv-group.bsky.social
August 28, 2025 at 2:00 PM
The Dutch Physics Council just circulated their biannual newsletter. Not yet registered? Sign up here: dutchphysicscouncil.nl/news/#newsle...
Amongst others we share the interview published in het Nederlands Tijdschrift voor #Natuurkunde with our chair Esther Alarcón Lladó @3dpv-group.bsky.social
Amongst others we share the interview published in het Nederlands Tijdschrift voor #Natuurkunde with our chair Esther Alarcón Lladó @3dpv-group.bsky.social
@loreoliv.bsky.social et al. :)
Johannes Hohlbein @hohlbeinlab.bsky.social from Wageningen presenting his great work on the dynamics of the E. coli replication initiator DnaA #SMLMS2025
August 28, 2025 at 8:58 AM
@loreoliv.bsky.social et al. :)
I will arrive in time for #SMLMS2025. Deutsche Bahn: Hold my beer. 🍺
August 27, 2025 at 9:06 AM
I will arrive in time for #SMLMS2025. Deutsche Bahn: Hold my beer. 🍺
Opus Magnum by @loreoliv.bsky.social on the role of DnaA in bacterial DNA replication initiation building on fancy combinations of #sptPALM, proteomics, flow cytometry, genetics, and other techniques. Great collab with the Staals lab and many others. Long 🧵👇
🧵 Our study on the elusive titration control mechanism of the replication initiator DnaA in Escherichia coli is now out on @natcomms.nature.com! @stephkoe.bsky.social
@hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social @ettema.bsky.social @mib-wur.bsky.social
doi.org/10.1038/s414...
@hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social @ettema.bsky.social @mib-wur.bsky.social
doi.org/10.1038/s414...
The Escherichia coli replication initiator DnaA is titrated on the chromosome - Nature Communications
A DNA-binding protein, DnaA, regulates the initiation of chromosomal replication in bacteria. Here, Olivi et al. show that the bacterial chromosome sequesters DnaA in a growth rate-dependent manner, s...
doi.org
August 22, 2025 at 9:03 AM
Opus Magnum by @loreoliv.bsky.social on the role of DnaA in bacterial DNA replication initiation building on fancy combinations of #sptPALM, proteomics, flow cytometry, genetics, and other techniques. Great collab with the Staals lab and many others. Long 🧵👇