Sergio Cruz-León
@sergiocruzleon.bsky.social
Theoretical and computational biophysics | Postdoc at Hummer's lab
@hummerlab.bsky.social | PhD at @mpibp.bsky.social | MSc. @unistuttgart.bsky.social | Physics at @uniandes.bsky.social 🇨🇴 🇩🇪 🇪🇺
@hummerlab.bsky.social | PhD at @mpibp.bsky.social | MSc. @unistuttgart.bsky.social | Physics at @uniandes.bsky.social 🇨🇴 🇩🇪 🇪🇺
Pinned
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
Reposted by Sergio Cruz-León
SNDing proteins into the membrane! Our new publication from @melaniemcdowell.bsky.social ’s group identifies the SND3 protein as a new route for membrane protein insertion! 🍄 📘 Read more here: www.mpg.de/25599408/102... Image: Louise Duever.
October 30, 2025 at 8:55 AM
SNDing proteins into the membrane! Our new publication from @melaniemcdowell.bsky.social ’s group identifies the SND3 protein as a new route for membrane protein insertion! 🍄 📘 Read more here: www.mpg.de/25599408/102... Image: Louise Duever.
Reposted by Sergio Cruz-León
Deep learning-based interpolation using cryoTIGER developed by the authors in this new Communications Biology manuscript enhances cryoET reconstructions. @mpibp.bsky.social
@becklab.bsky.social
@jpkreysing.bsky.social
@sergiocruzleon.bsky.social
doi.org/10.1038/s420...
@becklab.bsky.social
@jpkreysing.bsky.social
@sergiocruzleon.bsky.social
doi.org/10.1038/s420...
cryoTIGER: deep-learning based tilt interpolation generator for enhanced reconstruction in cryo electron tomography - Communications Biology
Deep learning-based interpolation using cryoTIGER enhances angular sampling in cryoET, improving 3D reconstructions, particle localization, and structural recovery without increasing electron dose.
doi.org
October 15, 2025 at 5:43 AM
Deep learning-based interpolation using cryoTIGER developed by the authors in this new Communications Biology manuscript enhances cryoET reconstructions. @mpibp.bsky.social
@becklab.bsky.social
@jpkreysing.bsky.social
@sergiocruzleon.bsky.social
doi.org/10.1038/s420...
@becklab.bsky.social
@jpkreysing.bsky.social
@sergiocruzleon.bsky.social
doi.org/10.1038/s420...
Reposted by Sergio Cruz-León
🎉 Congratulations to our postdoc @sergiocruzleon.bsky.social for receiving the 2026 Theory & Computation Postdoctoral Research Award from the Biophysical Society! 🎉 Sergio will present his work at the Theory & Computation subgroup Symposium during the 2026 Biophysical Society meeting.
September 29, 2025 at 2:25 PM
🎉 Congratulations to our postdoc @sergiocruzleon.bsky.social for receiving the 2026 Theory & Computation Postdoctoral Research Award from the Biophysical Society! 🎉 Sergio will present his work at the Theory & Computation subgroup Symposium during the 2026 Biophysical Society meeting.
Reposted by Sergio Cruz-León
Ready to lead pioneering research that bridges systems-level investigations of biological systems to molecular mechanism? The EMBL Molecular Systems Biology Unit in Heidelberg is hiring a Group Leader!
Group Leader – Molecular Systems Biology Unit
Are you ready to lead groundbreaking research in Molecular Systems Biology? Join us at EMBL! We are seeking a motivated scientist to lead an independent research group, addressing original biological ...
embl.wd103.myworkdayjobs.com
July 30, 2025 at 3:15 PM
Ready to lead pioneering research that bridges systems-level investigations of biological systems to molecular mechanism? The EMBL Molecular Systems Biology Unit in Heidelberg is hiring a Group Leader!
Reposted by Sergio Cruz-León
That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110
biorxiv.org/content/10.110
biorxiv.org
July 23, 2025 at 9:43 PM
That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110
biorxiv.org/content/10.110
Reposted by Sergio Cruz-León
Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...
July 12, 2025 at 12:49 AM
Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...
Reposted by Sergio Cruz-León
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
April 11, 2025 at 9:04 AM
New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
April 11, 2025 at 8:35 AM
Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social
🔗 Preprint here tinyurl.com/3a74uanv
Reposted by Sergio Cruz-León
Our paper on prediction of phase-separation propensities of disordered proteins from sequence is now published:
www.pnas.org/doi/10.1073/...
The paper has been substantially updated compared to the preprint including new experimental data and using the neural network to finetune CALVADOS. 1/n
www.pnas.org/doi/10.1073/...
The paper has been substantially updated compared to the preprint including new experimental data and using the neural network to finetune CALVADOS. 1/n
March 25, 2025 at 5:54 PM
Our paper on prediction of phase-separation propensities of disordered proteins from sequence is now published:
www.pnas.org/doi/10.1073/...
The paper has been substantially updated compared to the preprint including new experimental data and using the neural network to finetune CALVADOS. 1/n
www.pnas.org/doi/10.1073/...
The paper has been substantially updated compared to the preprint including new experimental data and using the neural network to finetune CALVADOS. 1/n
Reposted by Sergio Cruz-León
✨✨ It's #glycotime for #HIV Env ✨✨
N-linked glycans modulate flexibility & MPER epitope exposure #glycotime
Huge effort by @shehata92.bsky.social @lcasalino88.bsky.social with cryoET of Env in VLPs by @thevillalab.bsky.social & team 💪
Would love feedback!
www.biorxiv.org/content/10.1...
N-linked glycans modulate flexibility & MPER epitope exposure #glycotime
Huge effort by @shehata92.bsky.social @lcasalino88.bsky.social with cryoET of Env in VLPs by @thevillalab.bsky.social & team 💪
Would love feedback!
www.biorxiv.org/content/10.1...
March 28, 2025 at 4:23 AM
✨✨ It's #glycotime for #HIV Env ✨✨
N-linked glycans modulate flexibility & MPER epitope exposure #glycotime
Huge effort by @shehata92.bsky.social @lcasalino88.bsky.social with cryoET of Env in VLPs by @thevillalab.bsky.social & team 💪
Would love feedback!
www.biorxiv.org/content/10.1...
N-linked glycans modulate flexibility & MPER epitope exposure #glycotime
Huge effort by @shehata92.bsky.social @lcasalino88.bsky.social with cryoET of Env in VLPs by @thevillalab.bsky.social & team 💪
Would love feedback!
www.biorxiv.org/content/10.1...
Reposted by Sergio Cruz-León
Job alert: Join us in Mainz as
Max Planck Research Group Leader (W2) in Molecular Design
...and make your own research dreams happen on de novo design, generative models, proteins, materials ...
tinyurl.com/r2xjxnuk
@mpip-mainz.mpg.de
Max Planck Research Group Leader (W2) in Molecular Design
...and make your own research dreams happen on de novo design, generative models, proteins, materials ...
tinyurl.com/r2xjxnuk
@mpip-mainz.mpg.de
Max Planck Research Group Leader (W2) in Molecular Design
We are looking for exceptional early-career scientists conducting computational research with a proven record of accomplishment. The primary focus of this call is on candidates proposing research on b...
tinyurl.com
April 4, 2025 at 4:00 PM
Job alert: Join us in Mainz as
Max Planck Research Group Leader (W2) in Molecular Design
...and make your own research dreams happen on de novo design, generative models, proteins, materials ...
tinyurl.com/r2xjxnuk
@mpip-mainz.mpg.de
Max Planck Research Group Leader (W2) in Molecular Design
...and make your own research dreams happen on de novo design, generative models, proteins, materials ...
tinyurl.com/r2xjxnuk
@mpip-mainz.mpg.de
Reposted by Sergio Cruz-León
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
April 9, 2025 at 1:07 PM
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
Reposted by Sergio Cruz-León
Excited to share our latest work on nuclear pore complex scaffold integrity and its perturbation. Now out in Nature Cell Biology: rdcu.be/eg4i9
Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳
Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳
Nuclear pores safeguard the integrity of the nuclear envelope
Nature Cell Biology - Taniguchi et al. structurally analyse nuclear pore complex architecture in situ during differentiation, which is associated with mechanical constraints on the nuclear...
rdcu.be
April 9, 2025 at 3:49 PM
Excited to share our latest work on nuclear pore complex scaffold integrity and its perturbation. Now out in Nature Cell Biology: rdcu.be/eg4i9
Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳
Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳
Reposted by Sergio Cruz-León
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
March 5, 2025 at 4:02 PM
Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?
Reposted by Sergio Cruz-León
Interested in #RNA modelling across multiple scales? Join us for the @cecamevents.bsky.social meeting at SISSA, Trieste, May 19-22! www.cecam.org/workshop-det... We still have slots for contributed talks. Deadline March 23. Co-organized with @magistratolab.bsky.social and @marcodevivo.bsky.social
CECAM - RNA modelling across scales
www.cecam.org
February 20, 2025 at 7:22 PM
Interested in #RNA modelling across multiple scales? Join us for the @cecamevents.bsky.social meeting at SISSA, Trieste, May 19-22! www.cecam.org/workshop-det... We still have slots for contributed talks. Deadline March 23. Co-organized with @magistratolab.bsky.social and @marcodevivo.bsky.social
Reposted by Sergio Cruz-León
🤩 Highly optimized software running on a SOA #HPC cluster provides sufficient #computing power to #simulate #biomolecular systems at the mesoscale of viruses and organelles, and potentially small cells in the near future... Want to know more? Check out our paper 📑 in #JCC 🔗 doi.org/10.1002/jcc....
February 18, 2025 at 1:34 PM
🤩 Highly optimized software running on a SOA #HPC cluster provides sufficient #computing power to #simulate #biomolecular systems at the mesoscale of viruses and organelles, and potentially small cells in the near future... Want to know more? Check out our paper 📑 in #JCC 🔗 doi.org/10.1002/jcc....
Reposted by Sergio Cruz-León
Excited to see work led by @autophazeke.bsky.social, @minghaochen.bsky.social, Thanh Nguyen, and Ainara Claveras out in @science.org today, the culmination of a 15-year project to understand structurally how the synthesis of the critical lipid of autophagy, PI3P, is regulated. tinyurl.com/ywvjh8u4
February 6, 2025 at 7:51 PM
Excited to see work led by @autophazeke.bsky.social, @minghaochen.bsky.social, Thanh Nguyen, and Ainara Claveras out in @science.org today, the culmination of a 15-year project to understand structurally how the synthesis of the critical lipid of autophagy, PI3P, is regulated. tinyurl.com/ywvjh8u4
Reposted by Sergio Cruz-León
Exciting green news! 🦠 Do you know how certain microorganisms make a living by transforming CO2 into useful molecules? Check out our new work on the molecular choreography behind acetyl-CoA synthesis in the CODH/ACS protein complex. 👩🔬 🔗 www.biophys.mpg.de/2844976/micr...
January 31, 2025 at 1:14 PM
Exciting green news! 🦠 Do you know how certain microorganisms make a living by transforming CO2 into useful molecules? Check out our new work on the molecular choreography behind acetyl-CoA synthesis in the CODH/ACS protein complex. 👩🔬 🔗 www.biophys.mpg.de/2844976/micr...
Reposted by Sergio Cruz-León
I am very happy to share our latest work on the detailed cryo-EM structure of the NDH-PSI supercomplex of spinach. Thanks to Werner Kühlbrandt, Alex Hahn and @mpibp.bsky.social ! 🍃❄️🔬
You can find the paper in open access in Nature Structural and Molecular Biology:
www.nature.com/articles/s41...
You can find the paper in open access in Nature Structural and Molecular Biology:
www.nature.com/articles/s41...
Cryo-EM structure of the NDH–PSI–LHCI supercomplex from Spinacia oleracea - Nature Structural & Molecular Biology
Here, the authors report the structure of the nicotinamide adenine dinucleotide phosphate dehydrogenase–photosystem I–light-harvesting complex I supercomplex, providing insights into subunit interacti...
www.nature.com
January 27, 2025 at 12:18 PM
I am very happy to share our latest work on the detailed cryo-EM structure of the NDH-PSI supercomplex of spinach. Thanks to Werner Kühlbrandt, Alex Hahn and @mpibp.bsky.social ! 🍃❄️🔬
You can find the paper in open access in Nature Structural and Molecular Biology:
www.nature.com/articles/s41...
You can find the paper in open access in Nature Structural and Molecular Biology:
www.nature.com/articles/s41...
Reposted by Sergio Cruz-León
In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
January 17, 2025 at 6:43 PM
In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
Reposted by Sergio Cruz-León
Our research presented at @freieuniversitaet.bsky.social ! @bussigio.bsky.social will give a CRC colloquium presenting our recent applications of integrative methods and enhanced sampling to #RNA dynamics.
Host: @cecclementi.bsky.social
www.mi.fu-berlin.de/en/sfb1114/e...
Host: @cecclementi.bsky.social
www.mi.fu-berlin.de/en/sfb1114/e...
January Colloquium
www.mi.fu-berlin.de
January 16, 2025 at 8:02 AM
Our research presented at @freieuniversitaet.bsky.social ! @bussigio.bsky.social will give a CRC colloquium presenting our recent applications of integrative methods and enhanced sampling to #RNA dynamics.
Host: @cecclementi.bsky.social
www.mi.fu-berlin.de/en/sfb1114/e...
Host: @cecclementi.bsky.social
www.mi.fu-berlin.de/en/sfb1114/e...
Reposted by Sergio Cruz-León
PhDs in biochemistry, structural biology, pharmacology, or similar, located in the US and with strong publication track records and a high motivation to solve Parkinson's, please send CV directly to me at jimhurley@berkeley.edu.
Repost appreciated.
Repost appreciated.
January 9, 2025 at 2:58 AM
PhDs in biochemistry, structural biology, pharmacology, or similar, located in the US and with strong publication track records and a high motivation to solve Parkinson's, please send CV directly to me at jimhurley@berkeley.edu.
Repost appreciated.
Repost appreciated.
Reposted by Sergio Cruz-León
Check out our latest publication in @naturecellbiology.bsky.social on the role of phase separation in selective autophagy initiation! This was done in a fantastic collaboration with
@kraftlabfr.bsky.social
www.nature.com/articles/s41...
Short teaser below 👇👇👇
@kraftlabfr.bsky.social
www.nature.com/articles/s41...
Short teaser below 👇👇👇
Phase separation of initiation hubs on cargo is a trigger switch for selective autophagy - Nature Cell Biology
Licheva, Pflaum, Babic, Mancilla et al. show that low-affinity cargo–receptor interactions promote autophagy receptor mobility and condensation of the scaffold protein Atg11 to trigger initiation hub ...
www.nature.com
January 8, 2025 at 3:11 PM
Check out our latest publication in @naturecellbiology.bsky.social on the role of phase separation in selective autophagy initiation! This was done in a fantastic collaboration with
@kraftlabfr.bsky.social
www.nature.com/articles/s41...
Short teaser below 👇👇👇
@kraftlabfr.bsky.social
www.nature.com/articles/s41...
Short teaser below 👇👇👇
Reposted by Sergio Cruz-León
Molecular simulations or AI models produce ever larger and more complex datasets. Comparing different conditions to find meaningful patterns is a bottleneck for many projects.
We address this with PENSA, a flexible open-source analysis library.
Now out in JCP:
doi.org/10.1063/5.02...
🧪 #CompChem
We address this with PENSA, a flexible open-source analysis library.
Now out in JCP:
doi.org/10.1063/5.02...
🧪 #CompChem
Systematic analysis of biomolecular conformational ensembles with PENSA
Atomic-level simulations are widely used to study biomolecules and their dynamics. A common goal in such studies is to compare simulations of a molecular system
doi.org
January 7, 2025 at 9:07 AM
Molecular simulations or AI models produce ever larger and more complex datasets. Comparing different conditions to find meaningful patterns is a bottleneck for many projects.
We address this with PENSA, a flexible open-source analysis library.
Now out in JCP:
doi.org/10.1063/5.02...
🧪 #CompChem
We address this with PENSA, a flexible open-source analysis library.
Now out in JCP:
doi.org/10.1063/5.02...
🧪 #CompChem
Reposted by Sergio Cruz-León
Exciting work by Martin Beck’s group @mpibp.bsky.social providing further evidence for nuclear pores responding to mechanical stress. The work includes some nice modeling estimating the fluid flow across nuclear pores as a function of (osmotic) pressure.
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
Nuclear pore permeability and fluid flow are modulated by its dilation state
Hoffmann et al. describe within cells how the Dictyostelium discoideum nuclear pore complex structure constricts and dilates as a consequence of osmotic stress. Based on their experimental findings, they propose a hydrodynamics model and quantify fluid flow across nuclear pore complexes under conditions when nuclei change their volume rapidly.
www.cell.com
December 29, 2024 at 8:50 AM
Exciting work by Martin Beck’s group @mpibp.bsky.social providing further evidence for nuclear pores responding to mechanical stress. The work includes some nice modeling estimating the fluid flow across nuclear pores as a function of (osmotic) pressure.
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...