Bacterial bloodstream infections (BSIs) represent a huge burden for modern healthcare. BSIs caused by Pseudomonas aeruginosa exhibit the highest mortality rate (1, 2). This leading nosocomial pathogen causes various primary infections, such as burn wounds, pulmonary and urinary tract infections (3). Using its arsenal of virulence factors, it is able to breach both epithelial and endothelial barriers ([4–7], reviewed in [8]). Once in the bloodstream, P. aeruginosa faces the host immune response. In a previous study, we showed the complement system to be the main innate immune component responsible for the elimination of P. aeruginosa in the blood (9). The complement system is an enzymatic cascade of more than 30 proteins, whose activation results in the formation of the membrane attack complex (MAC) in the outer membrane of the target pathogen. Complement-mediated killing happens through three main steps including complement activation, assembly of the C5b-9 MAC on the outer membrane, and penetration of the MAC into the bilayer, eventually leading to bacterial lysis (10, 11). P. aeruginosa evolved strategies to evade those three steps. The secretion of proteases including AprA and LasB, cleaving C1, C2, and C3 or the secretion of ecotin, a protease inhibitor, leads to the blockade of complement activation (12–14). P. aeruginosa can also recruit host complement inhibitors to its surface, notably through the exposure of the elongation factor Tuf or the dihydrolipoamide dehydrogenase Lpd ([15, 16], reviewed in [8]). These immune evasion proteins can recruit the Factor H family of complement regulators, resulting in C3b degradation and preventing downstream proteolysis cascade. Finally, P. aeruginosa can modify its surface by producing exopolysaccharides (EPSs) such as alginate. When acylated, alginates decrease bacterial opsonization by the C3b molecule (17, 18).

Processes that occur after death have not received the same level of attention as the mechanisms of life. In this study, the authors show that bacteria have potentially evolved an altruistic trait to auto-degrade after death, thus permitting population nutrient recycling.

Biofilms are three-dimensional structures containing one or more bacterial species embedded in extracellular polymeric substances. Although most bi...

Pseudomonas syringae can detect and kill microbial predators via a chemical radar (Cra), which also allows the bacterium to infect plants despite predation pressure.

Single-cell gene expression analysis reveals phenotypic heterogeneity to enable bacterial specialization over the course of plant colonization.

InducTn-seq, a method for inducible mutagenesis followed by transposon insertion site sequencing, enables temporal control of transposition to bypass population bottlenecks and enable the quantificati...

The molecule targets bacteria in a way that other drugs don’t.

Pseudomonas species are ubiquitous in the environment and serve as valuable source of enzymes and secondary metabolites for industrial applications. P. aeruginosa secretes metalloproteases such as ela...

The Institut Pasteur launches an international call to recruit new junior research group leaders in the application of cutting-edge transdisciplinary approaches to the exploration of host-microbe, tis...

O-specific polysaccharide was immunogenic in patients with K pneumoniae bloodstream infection, supporting its potential as a candidate vaccine antigen. The cross-reactivity observed between similar O-...