Filipe Carvalho
@ffchcarvalho.bsky.social
Postdoc @ EpiMiC lab (UMR1319 Micalis, INRAE, Jouy-en-Josas 🇨🇵).
Previously @ Cossart lab (Institut Pasteur, Paris 🇨🇵), @ Cabanes lab (i3S, Porto 🇵🇹).
Molecular microbiology, host-pathogen interactions, bacterial dormancy and environmental persistence
Previously @ Cossart lab (Institut Pasteur, Paris 🇨🇵), @ Cabanes lab (i3S, Porto 🇵🇹).
Molecular microbiology, host-pathogen interactions, bacterial dormancy and environmental persistence
Pinned
Filipe Carvalho
@ffchcarvalho.bsky.social
· Nov 11
Hey #BlueSky!
I'm a Portuguese #microbiologist based in France, and my current research is focused on understanding the long-term #persistence of the bacterial #pathogen #Listeria monocytogenes in its environment.
Happy to follow and be followed around here (not in a creepy way 😆)
I'm a Portuguese #microbiologist based in France, and my current research is focused on understanding the long-term #persistence of the bacterial #pathogen #Listeria monocytogenes in its environment.
Happy to follow and be followed around here (not in a creepy way 😆)
Reposted by Filipe Carvalho
Many spores from the Firmicutes group are decorated with hair-like structures. Since the 60s, they remained a mystery. @remaut-lab.bsky.social named these fibres ENdospore Appendages (ENAs). Using Cryo-ID, we report the identity, structure and function of F-ENA.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes - Nature Communications
B. thuringiensis spores contain uncharacterized protein filaments that extend from the surface of the exosporium. Here, the authors show that these filaments feature conserved β-barrel neck domains an...
www.nature.com
August 17, 2025 at 5:52 AM
Many spores from the Firmicutes group are decorated with hair-like structures. Since the 60s, they remained a mystery. @remaut-lab.bsky.social named these fibres ENdospore Appendages (ENAs). Using Cryo-ID, we report the identity, structure and function of F-ENA.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
RagB stimulates the activity of the peptidoglycan polymerase RodA in Bacillus subtilis
[RagB = YrrS]
@emboreports.org by Frédérique Pompeo et al
www.embopress.org/doi/full/10....
[RagB = YrrS]
@emboreports.org by Frédérique Pompeo et al
www.embopress.org/doi/full/10....
RagB stimulates the activity of the peptidoglycan polymerase RodA in Bacillus subtilis | EMBO reports
imageimageRagB regulates the SEDS protein RodA in Bacillus subtilis, by interacting with RodA and by stimulating its activity. This regulation is essential
for cell wall synthesis, especially when Cla...
www.embopress.org
August 17, 2025 at 1:19 PM
Reposted by Filipe Carvalho
Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis
@commsbio.nature.com from @diepold-lab.bsky.social at @mpimicrobiomarburg.bsky.social
with Victor Sourjik, Daniel Unterweger
www.nature.com/articles/s42...
@commsbio.nature.com from @diepold-lab.bsky.social at @mpimicrobiomarburg.bsky.social
with Victor Sourjik, Daniel Unterweger
www.nature.com/articles/s42...
Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis - Communications Biology
Pseudomonas aeruginosa coordinates several virulence factors – the type 3 and H1-6 secretion systems and the flagellum – on a single cell level using the second messenger cyclic di-GMP, promoting subp...
www.nature.com
August 17, 2025 at 10:30 AM
Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis
@commsbio.nature.com from @diepold-lab.bsky.social at @mpimicrobiomarburg.bsky.social
with Victor Sourjik, Daniel Unterweger
www.nature.com/articles/s42...
@commsbio.nature.com from @diepold-lab.bsky.social at @mpimicrobiomarburg.bsky.social
with Victor Sourjik, Daniel Unterweger
www.nature.com/articles/s42...
Impact of Bacterial Membrane Vesicles on Cellular Responses in Leishmania amazonensis-Infected Macrophages In Vitro https://www.biorxiv.org/content/10.1101/2025.07.08.663775v1
July 9, 2025 at 7:35 AM
Effects of CwlM, a peptidoglycan synthesis regulator, on beta-lactam resistance and host-pathogen interactions https://www.biorxiv.org/content/10.1101/2025.07.08.663695v1
July 9, 2025 at 7:35 AM
A periplasmic protein complex mediates cell envelope integrity and intrinsic multidrug resistance in Mycobacterium tuberculosis https://www.biorxiv.org/content/10.1101/2025.07.08.663683v1
July 9, 2025 at 7:34 AM
New review from our lab! Compartmentalization during bacterial spore formation. Focusing on latest discoveries in Bacillus subtilis #subtiwiki
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
July 9, 2025 at 7:25 AM
Reposted by Filipe Carvalho
This paper advances deep proteome sequencing, improving detection of protein variants & isoforms beyond standard shotgun proteomics. By analyzing six human cell lines, it identifies a million unique peptides from 17,717 protein groups, highlighting translation of most alternatively spliced isoforms.
Global detection of human variants and isoforms by deep proteome sequencing - Nature Biotechnology
Deep proteome sequencing achieves ~80% coverage of the human proteome.
doi.org
June 1, 2025 at 1:18 PM
This paper advances deep proteome sequencing, improving detection of protein variants & isoforms beyond standard shotgun proteomics. By analyzing six human cell lines, it identifies a million unique peptides from 17,717 protein groups, highlighting translation of most alternatively spliced isoforms.
Discovery of a bicyclic peptide inhibitor of cell wall biosynthesis in the Enterobacterales demonstrates applicability of a modified phage display platform to antibiotic discovery and development.
www.nature.com/articles/s42...
www.nature.com/articles/s42...
May 30, 2025 at 11:06 AM
Reposted by Filipe Carvalho
Our new paper on FtsN is out! Now there are three tracks for organized cell wall constriction-- the old, boring E. coli always manages to surprise us.
A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.
www.nature.com/articles/s41...
A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.
www.nature.com/articles/s41...
Third track model for coordination of septal peptidoglycan synthesis and degradation by FtsN in Escherichia coli - Nature Microbiology
Partitioning of FtsN and the septal peptidoglycan synthesis complex between an active synthesis track and an inactive denuded glycan track coordinates opposing peptidoglycan synthesis and degradation ...
www.nature.com
May 27, 2025 at 5:13 PM
Our new paper on FtsN is out! Now there are three tracks for organized cell wall constriction-- the old, boring E. coli always manages to surprise us.
A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.
www.nature.com/articles/s41...
A special shout-out to all the people who made this work possible, especially Dr. Jason Lyu, and Dr. David Weiss.
www.nature.com/articles/s41...
Reposted by Filipe Carvalho
Bacterial extracellular vesicles target different bacterial species, impairing cell division and diminishing their pathogenicity #microsky
May 28, 2025 at 7:24 AM
Bacterial extracellular vesicles target different bacterial species, impairing cell division and diminishing their pathogenicity #microsky
A Salmonella type I toxin promotes systemic infection by inhibiting FoF1 ATP synthase https://www.biorxiv.org/content/10.1101/2025.05.26.656083v1
May 27, 2025 at 7:13 AM
Reposted by Filipe Carvalho
Complete 5-methylcytidine substitution enhances the efficacy of self-amplifying RNA go.nature.com/3RYbPHK
rdcu.be/em3ZT
rdcu.be/em3ZT
Complete substitution with modified nucleotides in self-amplifying RNA suppresses the interferon response and increases potency - Nature Biotechnology
Complete 5-methylcytidine substitution enhances the efficacy of self-amplifying RNA.
go.nature.com
May 27, 2025 at 3:26 AM
Complete 5-methylcytidine substitution enhances the efficacy of self-amplifying RNA go.nature.com/3RYbPHK
rdcu.be/em3ZT
rdcu.be/em3ZT
A Conserved Mycobacterial Nucleomodulin Hijacks the Host COMPASS Complex to Reprogram Pro-Inflammatory Transcription and Promote Intracellular Survival https://www.biorxiv.org/content/10.1101/2025.05.21.655295v1
May 22, 2025 at 7:30 AM
Intralysosomal pathogens differentially influence the proteolytic potential of their niche https://www.biorxiv.org/content/10.1101/2025.05.21.655381v1
May 22, 2025 at 7:29 AM
Reposted by Filipe Carvalho
How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z
May 6, 2025 at 12:46 PM
How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z
Reposted by Filipe Carvalho
Out in Nature today: A new immune signaling molecule, His-ADPR, is produced by defensive TIR domain proteins in bacteria to protect from phage
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
April 30, 2025 at 6:44 PM
Out in Nature today: A new immune signaling molecule, His-ADPR, is produced by defensive TIR domain proteins in bacteria to protect from phage
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
Joint work with the Tamulaitienė and Kranzusch labs
www.nature.com/articles/s41...
Congrats Carmel Avraham, Dziugas Sabonis, Renee Chang and co-authors!
Reposted by Filipe Carvalho
The bacteria that causes Q FEVER with some elegant NICHE ENGINEERING!
𝘊𝘰𝘹𝘪𝘦𝘭𝘭𝘢 𝘣𝘶𝘳𝘯𝘦𝘵𝘪𝘪 survives in a hostile PHAGOLYSOME by kicking out key host protease CATHEPSIN B
With Dot/Icm secretion and retrograde trafficking, it remodels its vacuole into a friendlier home
www.nature.com/articles/s41...
𝘊𝘰𝘹𝘪𝘦𝘭𝘭𝘢 𝘣𝘶𝘳𝘯𝘦𝘵𝘪𝘪 survives in a hostile PHAGOLYSOME by kicking out key host protease CATHEPSIN B
With Dot/Icm secretion and retrograde trafficking, it remodels its vacuole into a friendlier home
www.nature.com/articles/s41...
April 27, 2025 at 3:01 AM
The bacteria that causes Q FEVER with some elegant NICHE ENGINEERING!
𝘊𝘰𝘹𝘪𝘦𝘭𝘭𝘢 𝘣𝘶𝘳𝘯𝘦𝘵𝘪𝘪 survives in a hostile PHAGOLYSOME by kicking out key host protease CATHEPSIN B
With Dot/Icm secretion and retrograde trafficking, it remodels its vacuole into a friendlier home
www.nature.com/articles/s41...
𝘊𝘰𝘹𝘪𝘦𝘭𝘭𝘢 𝘣𝘶𝘳𝘯𝘦𝘵𝘪𝘪 survives in a hostile PHAGOLYSOME by kicking out key host protease CATHEPSIN B
With Dot/Icm secretion and retrograde trafficking, it remodels its vacuole into a friendlier home
www.nature.com/articles/s41...
Discovery of a novel antibiotic class targeting the enolase of Acinetobacterbaumannii https://www.biorxiv.org/content/10.1101/2025.04.23.650324v1
April 25, 2025 at 7:37 AM
Undecaprenyl Pyrophosphate Phosphatase (UppP) is a pivotal element in Salmonella Intramacrophage Survival https://www.biorxiv.org/content/10.1101/2025.04.24.650467v1
April 25, 2025 at 7:36 AM
Reposted by Filipe Carvalho
Check it out - amazing new work by @raflynn5.bsky.social and Kostas Tzelepis labs, discovery of a cell surface RNA/glycoRNA binding protein that is also a cancer antigen, new target for antibody therapy!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Treatment of acute myeloid leukemia models by targeting a cell surface RNA-binding protein - Nature Biotechnology
An RNA-binding protein on leukemia cells provides an effective target in mouse models.
www.nature.com
April 25, 2025 at 5:19 AM
Check it out - amazing new work by @raflynn5.bsky.social and Kostas Tzelepis labs, discovery of a cell surface RNA/glycoRNA binding protein that is also a cancer antigen, new target for antibody therapy!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Excited to share this story from my PhD, out today in Nature Communications! We show that Coxiella removes cathepsin B to reduce lysosomal hostility and promote bacterial success. This depends on a vacuole established by effector CvpB.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Coxiella burnetii manipulates the lysosomal protease cathepsin B to facilitate intracellular success - Nature Communications
The zoonotic pathogen Coxiella burnetiiestablishes a unique intracellular niche within a lysosome-derived vacuole. Here Bird et al. undertook proteomic, cell biology and microbiology approaches to cha...
www.nature.com
April 25, 2025 at 7:34 AM
Happy Freedom Day and 51st anniversary of the Carnation Revolution! 🇵🇹
April 25, 2025 at 7:28 AM
Happy Freedom Day and 51st anniversary of the Carnation Revolution! 🇵🇹