Amir Rahmani
@astormic.bsky.social
Super-resolution microscopy enthusiast
Postdoc at the University of Cambridge
Visiting Researcher at the British Antarctic Survey
College Research Associate at Wolfson College
https://laser.ceb.cam.ac.uk/
Postdoc at the University of Cambridge
Visiting Researcher at the British Antarctic Survey
College Research Associate at Wolfson College
https://laser.ceb.cam.ac.uk/
Crafting New Year (super-)resolutions at cold for 2026
Happy New Year everyone 🎉
Happy New Year everyone 🎉
January 1, 2026 at 12:33 AM
Crafting New Year (super-)resolutions at cold for 2026
Happy New Year everyone 🎉
Happy New Year everyone 🎉
Reposted by Amir Rahmani
Merry Christmas to all our students, staff, alumni and friends around the world 🎄
📸 Lloyd Mann
📸 Lloyd Mann
December 25, 2025 at 8:00 AM
Merry Christmas to all our students, staff, alumni and friends around the world 🎄
📸 Lloyd Mann
📸 Lloyd Mann
Reposted by Amir Rahmani
Bored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read!
Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.
www.biorxiv.org/content/10.6...
Happy holidays ✨🎄
Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.
www.biorxiv.org/content/10.6...
Happy holidays ✨🎄
December 24, 2025 at 7:18 AM
Bored over the holidays? Give our new preprint on how #microtubule lattice alteration by taxols can regulate #RhoA #signalling via GEF-H1 a read!
Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.
www.biorxiv.org/content/10.6...
Happy holidays ✨🎄
Perhaps a new mechanism of action for taxol #chemotherapeutics during interphase.
www.biorxiv.org/content/10.6...
Happy holidays ✨🎄
Reposted by Amir Rahmani
Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render)
@zeiss-microscopy.bsky.social
@zeiss-microscopy.bsky.social
December 24, 2025 at 1:16 PM
Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render)
@zeiss-microscopy.bsky.social
@zeiss-microscopy.bsky.social
Nice collection of cameras!
December 23, 2025 at 11:06 PM
Nice collection of cameras!
Reposted by Amir Rahmani
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
December 22, 2025 at 10:45 AM
✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!
I was incredibly fortunate in this! The ups and downs were many but all those discussions helped make sense of a few scientific questions that are still ongoing and lead to further questions.
Doing a PhD is - at heart - one long discussion with your mentor. The discussion changes over time - with unexpected turns and ups & downs - but through it all is a pair of people discussing a topic endlessly to make sense of it.
PhD students: choose someone you like to talk to!
PhD students: choose someone you like to talk to!
December 20, 2025 at 2:06 AM
I was incredibly fortunate in this! The ups and downs were many but all those discussions helped make sense of a few scientific questions that are still ongoing and lead to further questions.
Reposted by Amir Rahmani
Tracking coordinated cellular dynamics directly from images -- New method paper by labmates @bgraedel.bsky.social & @macdobry.bsky.social ! Python package & @napari.org plugin, all the good stuff:
Paper: doi.org/10.1242/jcs....
Code: github.com/pertzlab/arc...
Plugin: github.com/pertzlab/arc...
Paper: doi.org/10.1242/jcs....
Code: github.com/pertzlab/arc...
Plugin: github.com/pertzlab/arc...
December 18, 2025 at 4:16 PM
Tracking coordinated cellular dynamics directly from images -- New method paper by labmates @bgraedel.bsky.social & @macdobry.bsky.social ! Python package & @napari.org plugin, all the good stuff:
Paper: doi.org/10.1242/jcs....
Code: github.com/pertzlab/arc...
Plugin: github.com/pertzlab/arc...
Paper: doi.org/10.1242/jcs....
Code: github.com/pertzlab/arc...
Plugin: github.com/pertzlab/arc...
Reposted by Amir Rahmani
New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos.
www.nature.com/articles/s42...
www.nature.com/articles/s42...
December 15, 2025 at 10:24 AM
New paper out! Combining single-objective light-sheet microscopy and time-resolved SPAD array detection, we massively accelerate fluorescence lifetime imaging (FLIM) compared to confocal FLIM, making FLIM applicable to 3D specimen such as organoids and embryos.
www.nature.com/articles/s42...
www.nature.com/articles/s42...
Reposted by Amir Rahmani
My shirt is cooler than yours
December 11, 2025 at 7:06 PM
My shirt is cooler than yours
Fantastic night at Christ’s college for the LAG-MNG Christmas meal!
@lasergroup.bsky.social
@lasergroup.bsky.social
December 8, 2025 at 11:09 PM
Fantastic night at Christ’s college for the LAG-MNG Christmas meal!
@lasergroup.bsky.social
@lasergroup.bsky.social
I won’t buy a lens from you after seeing you put it upside down. 🙃
December 7, 2025 at 11:51 PM
I won’t buy a lens from you after seeing you put it upside down. 🙃
Nature’s optics at its best! A full lunar halo lighting up the night.
December 3, 2025 at 11:30 PM
Nature’s optics at its best! A full lunar halo lighting up the night.
Reposted by Amir Rahmani
Another huge advantage of miOPM is that it's gentle! A light-sheet microscope that's compatible with standard slides and multi-well plates!
-> Here we show 3D mitochondrial membrane potential in mammalian cells (GFP/RFP for mito, Cy5 for DNA). The cells move around freely!
doi.org/10.1101/2025...
-> Here we show 3D mitochondrial membrane potential in mammalian cells (GFP/RFP for mito, Cy5 for DNA). The cells move around freely!
doi.org/10.1101/2025...
November 9, 2025 at 8:03 PM
Another huge advantage of miOPM is that it's gentle! A light-sheet microscope that's compatible with standard slides and multi-well plates!
-> Here we show 3D mitochondrial membrane potential in mammalian cells (GFP/RFP for mito, Cy5 for DNA). The cells move around freely!
doi.org/10.1101/2025...
-> Here we show 3D mitochondrial membrane potential in mammalian cells (GFP/RFP for mito, Cy5 for DNA). The cells move around freely!
doi.org/10.1101/2025...
Reposted by Amir Rahmani
PhD studentship available on using our single-molecule flow cytometry technology to detect rare proteins. Apply if you're interested in applying single-molecule techniques to improve diagnostics and to discover new routes for anti-cancer immunotherapy. www.findaphd.com/phds/project...
Unraveling the biology and signalling potential of sparse cell antigens through single-molecule flow cytometry at University of Leeds on FindAPhD.com
PhD Project - Unraveling the biology and signalling potential of sparse cell antigens through single-molecule flow cytometry at University of Leeds, listed on FindAPhD.com
www.findaphd.com
November 6, 2025 at 5:07 PM
PhD studentship available on using our single-molecule flow cytometry technology to detect rare proteins. Apply if you're interested in applying single-molecule techniques to improve diagnostics and to discover new routes for anti-cancer immunotherapy. www.findaphd.com/phds/project...
Always there for our group meetings!
November 5, 2025 at 8:16 PM
Always there for our group meetings!
Reposted by Amir Rahmani
Single-molecule friends!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
2026 Single Molecule Approaches to Biology Conference GRC
The 2026 Gordon Research Conference on Single Molecule Approaches to Biology will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.
www.grc.org
November 2, 2025 at 7:23 PM
Single-molecule friends!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
Reposted by Amir Rahmani
Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries.
doi.org/10.1101/2025...
doi.org/10.1101/2025...
August 28, 2025 at 8:23 PM
Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries.
doi.org/10.1101/2025...
doi.org/10.1101/2025...
👀 What if your flow cytometer could see the invisible?
Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry.
www.biorxiv.org/content/10.1...
Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry.
www.biorxiv.org/content/10.1...
Single-molecule flow cytometry
Flow cytometry (FC) is a powerful tool for high-throughput characterisation of cell populations, yet its ensemble fluorescence detection and the autofluorescence of cells impose a sensitivity limit of...
www.biorxiv.org
August 27, 2025 at 6:39 AM
👀 What if your flow cytometer could see the invisible?
Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry.
www.biorxiv.org/content/10.1...
Our new preprint introduces single-molecule flow cytometry (smFC), pushing detection sensitivity 10–80× beyond conventional flow cytometry.
www.biorxiv.org/content/10.1...
Reposted by Amir Rahmani
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
August 19, 2025 at 7:31 PM
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
Reposted by Amir Rahmani
I was searching for a Python utility for Fourier Ring/Shell Correlation. All I got in a blue sky search were 4 small posts from @christletx.bsky.social ... all I got from chatgpt was installation instructions for non-existent libraries and github repos... anyone know a good tool for this?
August 16, 2025 at 3:08 PM
I was searching for a Python utility for Fourier Ring/Shell Correlation. All I got in a blue sky search were 4 small posts from @christletx.bsky.social ... all I got from chatgpt was installation instructions for non-existent libraries and github repos... anyone know a good tool for this?
Reposted by Amir Rahmani
The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...
June 27, 2025 at 3:41 PM
The review on live-cell SMT that I contribute to the jmolbiol.bsky.social special issue ‚Imaging of the central dogma‘ is now online as pre-proof: www.sciencedirect.com/science/arti...
Reposted by Amir Rahmani
Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.
July 9, 2025 at 8:00 PM
Production has started for a new #Snoutscope objective, AMS-AGY v3. It has significantly larger FOV and some other tweaks from v1 and v2. Currently accepting pre-orders. The price will need to increase modestly after deliveries start late October.
Reposted by Amir Rahmani
📽️ G&D Tapes 📽️
G&D author, Noah Helton tells us about their new study in #genesdev, revealing an intriguing link between stress granule formation and the integrated stress response. #OpenAccess @smslmoon.bsky.social
Read the full story here:
➡️ tinyurl.com/gd352899
G&D author, Noah Helton tells us about their new study in #genesdev, revealing an intriguing link between stress granule formation and the integrated stress response. #OpenAccess @smslmoon.bsky.social
Read the full story here:
➡️ tinyurl.com/gd352899
July 8, 2025 at 6:41 PM
📽️ G&D Tapes 📽️
G&D author, Noah Helton tells us about their new study in #genesdev, revealing an intriguing link between stress granule formation and the integrated stress response. #OpenAccess @smslmoon.bsky.social
Read the full story here:
➡️ tinyurl.com/gd352899
G&D author, Noah Helton tells us about their new study in #genesdev, revealing an intriguing link between stress granule formation and the integrated stress response. #OpenAccess @smslmoon.bsky.social
Read the full story here:
➡️ tinyurl.com/gd352899