Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries.

doi.org/10.1101/2025...

With smFC we achieve:
✅ Digital precision down to ~2–3 molecules/cell
✅ 10–80× improved detection limits vs. conventional FC
✅ Quantification of membrane proteins in high throughput, single-cell detail

smFC combines:
🔬 High-NA oblique plane microscopy (OPM)
💧 Microfluidics
✨ Superbright, large Stokes shift dyes
→ enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.