Amir Rahmani
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astormic.bsky.social
Amir Rahmani
@astormic.bsky.social
Super-resolution microscopy enthusiast
Postdoc at the University of Cambridge
Visiting Researcher at the British Antarctic Survey
College Research Associate at Wolfson College
https://laser.ceb.cam.ac.uk/
Any good silicone immersion one?
November 7, 2025 at 7:46 PM
My guess is that you would be able to get away with an adjustable slit to tune the NA!
November 5, 2025 at 8:33 PM
But does it have to be H-1B visa?
September 21, 2025 at 6:18 PM
Thank you! 🤓
August 30, 2025 at 6:12 AM
I’ve never had that privilege!
August 27, 2025 at 9:49 AM
I really need a keyboard blocker like this!
August 27, 2025 at 9:11 AM
Huge thanks to an incredible team who made this possible 🙌 especially Aleks for his great supervision and support.
August 27, 2025 at 6:39 AM
We believe smFC brings single-molecule sensitivity to scalable cell analysis.
Excited to see how this impacts biology & medicine!
Read the full story here 👇
www.biorxiv.org/content/10.1...
Single-molecule flow cytometry
Flow cytometry (FC) is a powerful tool for high-throughput characterisation of cell populations, yet its ensemble fluorescence detection and the autofluorescence of cells impose a sensitivity limit of...
www.biorxiv.org
August 27, 2025 at 6:39 AM
We applied smFC to triple-negative breast cancer (TNBC) cells:
📊 Found 30% of cells express low-abundance c-kit.
🧬 This subpopulation was invisible to standard flow cytometry, but smFC revealed it.
August 27, 2025 at 6:39 AM
With smFC we achieve:
✅ Digital precision down to ~2–3 molecules/cell
✅ 10–80× improved detection limits vs. conventional FC
✅ Quantification of membrane proteins in high throughput, single-cell detail
August 27, 2025 at 6:39 AM
smFC combines:
🔬 High-NA oblique plane microscopy (OPM)
💧 Microfluidics
✨ Superbright, large Stokes shift dyes
→ enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.
August 27, 2025 at 6:39 AM
Conventional flow cytometry is powerful but has a blind spot:
❌ It can’t detect proteins expressed at very low levels (<100–1000 molecules/cell).
That means many weak but biologically crucial signals are invisible.
August 27, 2025 at 6:39 AM
August 8, 2025 at 7:22 AM