Amir Rahmani
@astormic.bsky.social
Super-resolution microscopy enthusiast
Postdoc at the University of Cambridge
Visiting Researcher at the British Antarctic Survey
College Research Associate at Wolfson College
https://laser.ceb.cam.ac.uk/
Postdoc at the University of Cambridge
Visiting Researcher at the British Antarctic Survey
College Research Associate at Wolfson College
https://laser.ceb.cam.ac.uk/
Always there for our group meetings!
November 5, 2025 at 8:16 PM
Always there for our group meetings!
Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries.
doi.org/10.1101/2025...
doi.org/10.1101/2025...
August 28, 2025 at 8:23 PM
Thrilled to see interest in this preprint! This work was a major part of my PhD, my hope is that single-molecule flow cytometry can be useful to the community in exploring new applications and discoveries.
doi.org/10.1101/2025...
doi.org/10.1101/2025...
We applied smFC to triple-negative breast cancer (TNBC) cells:
📊 Found 30% of cells express low-abundance c-kit.
🧬 This subpopulation was invisible to standard flow cytometry, but smFC revealed it.
📊 Found 30% of cells express low-abundance c-kit.
🧬 This subpopulation was invisible to standard flow cytometry, but smFC revealed it.
August 27, 2025 at 6:39 AM
We applied smFC to triple-negative breast cancer (TNBC) cells:
📊 Found 30% of cells express low-abundance c-kit.
🧬 This subpopulation was invisible to standard flow cytometry, but smFC revealed it.
📊 Found 30% of cells express low-abundance c-kit.
🧬 This subpopulation was invisible to standard flow cytometry, but smFC revealed it.
With smFC we achieve:
✅ Digital precision down to ~2–3 molecules/cell
✅ 10–80× improved detection limits vs. conventional FC
✅ Quantification of membrane proteins in high throughput, single-cell detail
✅ Digital precision down to ~2–3 molecules/cell
✅ 10–80× improved detection limits vs. conventional FC
✅ Quantification of membrane proteins in high throughput, single-cell detail
August 27, 2025 at 6:39 AM
With smFC we achieve:
✅ Digital precision down to ~2–3 molecules/cell
✅ 10–80× improved detection limits vs. conventional FC
✅ Quantification of membrane proteins in high throughput, single-cell detail
✅ Digital precision down to ~2–3 molecules/cell
✅ 10–80× improved detection limits vs. conventional FC
✅ Quantification of membrane proteins in high throughput, single-cell detail
smFC combines:
🔬 High-NA oblique plane microscopy (OPM)
💧 Microfluidics
✨ Superbright, large Stokes shift dyes
→ enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.
🔬 High-NA oblique plane microscopy (OPM)
💧 Microfluidics
✨ Superbright, large Stokes shift dyes
→ enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.
August 27, 2025 at 6:39 AM
smFC combines:
🔬 High-NA oblique plane microscopy (OPM)
💧 Microfluidics
✨ Superbright, large Stokes shift dyes
→ enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.
🔬 High-NA oblique plane microscopy (OPM)
💧 Microfluidics
✨ Superbright, large Stokes shift dyes
→ enabling optical sectioning + photon efficiency needed for single-molecule detection in flowing cells.
Excellent choice of photo to begin the talk!
Speaker: Dr Meng Lu from Peking University
Speaker: Dr Meng Lu from Peking University
July 7, 2025 at 3:10 PM
Excellent choice of photo to begin the talk!
Speaker: Dr Meng Lu from Peking University
Speaker: Dr Meng Lu from Peking University
Need to play more with it but so far works great on this OPM data.
May 13, 2025 at 10:31 PM
Need to play more with it but so far works great on this OPM data.
spot = analysis.SpotDiagram(lens)
spot.view()
spot.view()
May 3, 2025 at 2:15 PM
spot = analysis.SpotDiagram(lens)
spot.view()
spot.view()
from optiland import analysis
from optiland.samples.objectives import CookeTriplet
lens = CookeTriplet()
lens.draw()
from optiland.samples.objectives import CookeTriplet
lens = CookeTriplet()
lens.draw()
May 3, 2025 at 2:11 PM
from optiland import analysis
from optiland.samples.objectives import CookeTriplet
lens = CookeTriplet()
lens.draw()
from optiland.samples.objectives import CookeTriplet
lens = CookeTriplet()
lens.draw()
A great question @superresolusian.bsky.social asked:
What’s the worst-looking image we can still analyse?
What’s the worst-looking image we can still analyse?
April 7, 2025 at 3:14 PM
A great question @superresolusian.bsky.social asked:
What’s the worst-looking image we can still analyse?
What’s the worst-looking image we can still analyse?
Hearing how they removed the glass from the camera chip to do OPM. Stressful indeed!
April 7, 2025 at 12:08 PM
Hearing how they removed the glass from the camera chip to do OPM. Stressful indeed!
The SIMple from @lasergroup.bsky.social
April 7, 2025 at 10:22 AM
The SIMple from @lasergroup.bsky.social
Lisa-Maria Needham presenting label-free study of single biomolecules using Fabry-Pérot microcavities. 👀
April 7, 2025 at 10:00 AM
Lisa-Maria Needham presenting label-free study of single biomolecules using Fabry-Pérot microcavities. 👀
It was a real pleasure to host Sabrina Simoncelli here in Leeds for the MNP/BioNano external seminar.
Super-resolution and DNA-PAINT results were amazing!
Super-resolution and DNA-PAINT results were amazing!
February 26, 2025 at 3:05 PM
It was a real pleasure to host Sabrina Simoncelli here in Leeds for the MNP/BioNano external seminar.
Super-resolution and DNA-PAINT results were amazing!
Super-resolution and DNA-PAINT results were amazing!
DNA-PAINT imaging with hydrogel imprinting and clearing
@jmstein.bsky.social
www.biorxiv.org/content/10.1...
@jmstein.bsky.social
www.biorxiv.org/content/10.1...
February 17, 2025 at 10:56 AM
DNA-PAINT imaging with hydrogel imprinting and clearing
@jmstein.bsky.social
www.biorxiv.org/content/10.1...
@jmstein.bsky.social
www.biorxiv.org/content/10.1...
From my attempts to do OPM with a tilted camera!
February 12, 2025 at 5:24 PM
From my attempts to do OPM with a tilted camera!
Any chance to get access to the file?
February 5, 2025 at 7:51 AM
Any chance to get access to the file?
Tripod was common!
February 3, 2025 at 1:31 PM
Tripod was common!
Is that one of those?
February 1, 2025 at 10:28 PM
Is that one of those?
