Joshua Hislop
@syncelljoshua.bsky.social
Graduate student in the Ebrahimkhani lab at the University of Pittsburgh.
Reposted by Joshua Hislop
Novel markers for haemogenic endothelium and haematopoietic progenitors in the mouse yolk sac https://www.biorxiv.org/content/10.1101/2025.11.21.689693v1
November 23, 2025 at 12:30 AM
Novel markers for haemogenic endothelium and haematopoietic progenitors in the mouse yolk sac https://www.biorxiv.org/content/10.1101/2025.11.21.689693v1
Reposted by Joshua Hislop
Unified Generation of Regionalized Neural Organoids from Single-Lumen Neuroepithelium https://www.biorxiv.org/content/10.1101/2025.11.18.689013v1
November 18, 2025 at 9:32 PM
Unified Generation of Regionalized Neural Organoids from Single-Lumen Neuroepithelium https://www.biorxiv.org/content/10.1101/2025.11.18.689013v1
Reposted by Joshua Hislop
If you’ve ever wondered why you can inject things into the yolk early and it affects the embryo, this phenomena is the reason it works! 👇 #zebrafish
#ZebrafishZunday: Cytoplasmic (or ooplasmic) streaming leads to the segregation of embryo from yolk granules. Credit to @shamipourshayan.bsky.social & @heisenbergcplab.bsky.social. 🧪
November 16, 2025 at 2:10 PM
If you’ve ever wondered why you can inject things into the yolk early and it affects the embryo, this phenomena is the reason it works! 👇 #zebrafish
Reposted by Joshua Hislop
Dismantling the Institutional Review Board (IRB), that oversees all human subjects research, is bad. Really bad.
The entire HR department at the CDC is gone. Everyone at the IRB, which makes sure studies are conducted ethically and rigorously, and the ethics office, which oversee conflicts of interest for CDC leaders and advisory committee members, were also let go.
www.theguardian.com/us-news/2025...
www.theguardian.com/us-news/2025...
‘Instability and confusion’ as CDC slashes 1,300 jobs before reinstating half
Trump administration says 700 notices were sent in error, while top CDC officer says ‘they didn’t think through what they were doing’
www.theguardian.com
October 19, 2025 at 4:00 PM
Dismantling the Institutional Review Board (IRB), that oversees all human subjects research, is bad. Really bad.
Reposted by Joshua Hislop
(1/8) Very Happy to share our lab’s 3D post-implantation human embryo model, made via signalling reprograming!
🙌 Huge thanks to the entire team and our collaborators, special shout-out to @cc0447.bsky.social (led the work) and @jinjin-0101.bsky.social (comp. analysis).
www.cell.com/cell-stem-ce...
🙌 Huge thanks to the entire team and our collaborators, special shout-out to @cc0447.bsky.social (led the work) and @jinjin-0101.bsky.social (comp. analysis).
www.cell.com/cell-stem-ce...
Signaling reprogramming via Stat3 activation unravels high-fidelity human post-implantation embryo modeling
Enhanced STAT3 activation combined with TGF-β inhibition reprograms human PSCs into
cells specifying all early embryonic lineages. Upon 3D suspension culture, these cells
self-organize within 6 days i...
www.cell.com
September 16, 2025 at 4:12 PM
(1/8) Very Happy to share our lab’s 3D post-implantation human embryo model, made via signalling reprograming!
🙌 Huge thanks to the entire team and our collaborators, special shout-out to @cc0447.bsky.social (led the work) and @jinjin-0101.bsky.social (comp. analysis).
www.cell.com/cell-stem-ce...
🙌 Huge thanks to the entire team and our collaborators, special shout-out to @cc0447.bsky.social (led the work) and @jinjin-0101.bsky.social (comp. analysis).
www.cell.com/cell-stem-ce...
Reposted by Joshua Hislop
YO, what's up with all these almost-witchcraft techniques?? In this paper, they develop a way to freeze cells so quickly that it preserves Calcium waves midway!! WHAT??? HELLO??? So now you can do 3D reconstruction of calcium waves with just a normal confocal. www.nature.com/articles/s41...
August 26, 2025 at 9:11 PM
YO, what's up with all these almost-witchcraft techniques?? In this paper, they develop a way to freeze cells so quickly that it preserves Calcium waves midway!! WHAT??? HELLO??? So now you can do 3D reconstruction of calcium waves with just a normal confocal. www.nature.com/articles/s41...
Reposted by Joshua Hislop
Our latest: We developed a chemo-optogenetic system for precise spatiotemporal control of morphogen production. Using dual light + small molecule control of Sonic Hedgehog production, we recapitulated neural tube patterning in vitro & measured spread of Shh
🧵
www.sciencedirect.com/science/arti...
🧵
www.sciencedirect.com/science/arti...
Investigating morphogen and patterning dynamics with optogenetic control of morphogen production
Morphogen gradients provide the patterning cues that instruct cell fate decisions during development. Here, we establish an optogenetic system for the…
www.sciencedirect.com
August 26, 2025 at 8:28 AM
Our latest: We developed a chemo-optogenetic system for precise spatiotemporal control of morphogen production. Using dual light + small molecule control of Sonic Hedgehog production, we recapitulated neural tube patterning in vitro & measured spread of Shh
🧵
www.sciencedirect.com/science/arti...
🧵
www.sciencedirect.com/science/arti...
Reposted by Joshua Hislop
"WASHINGTON, D.C. (August 1, 2025) – The Corporation for Public Broadcasting (CPB) announced today that it will begin an orderly wind-down of its operations"
cpb.org/pressroom/Co...
cpb.org/pressroom/Co...
August 1, 2025 at 4:59 PM
"WASHINGTON, D.C. (August 1, 2025) – The Corporation for Public Broadcasting (CPB) announced today that it will begin an orderly wind-down of its operations"
cpb.org/pressroom/Co...
cpb.org/pressroom/Co...
Reposted by Joshua Hislop
1
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!
🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!
🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...
July 27, 2025 at 5:26 PM
1
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!
🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...
To predict the behaviour of a primate, would you rather base your guess on a closely related species or one with a similar brain shape? We looked at brains & behaviours of 70 species, you’ll be surprised!
🧵Thread on our new preprint with @r3rt0.bsky.social , doi.org/10.1101/2025...
Reposted by Joshua Hislop
(1/10)
I am excited to finally share our work on avian left/right symmetry breaking with you. We reveal a tissue-scale active torque dipole generated at the Hensen’s node, thanks to crazy experiments by Julia @juliapfanzelter.bsky.social and some analysis by me.
doi.org/10.1101/2025...
I am excited to finally share our work on avian left/right symmetry breaking with you. We reveal a tissue-scale active torque dipole generated at the Hensen’s node, thanks to crazy experiments by Julia @juliapfanzelter.bsky.social and some analysis by me.
doi.org/10.1101/2025...
An active torque dipole across tissue layers drives avian left-right symmetry breaking
Unlike in mice, frogs, and fish, left-right (L/R) body axis formation in avian embryos does not arise from the chiral beat of cilia. Instead, a counter-clockwise tissue rotation around Hensen′ node, t...
doi.org
July 20, 2025 at 1:42 PM
(1/10)
I am excited to finally share our work on avian left/right symmetry breaking with you. We reveal a tissue-scale active torque dipole generated at the Hensen’s node, thanks to crazy experiments by Julia @juliapfanzelter.bsky.social and some analysis by me.
doi.org/10.1101/2025...
I am excited to finally share our work on avian left/right symmetry breaking with you. We reveal a tissue-scale active torque dipole generated at the Hensen’s node, thanks to crazy experiments by Julia @juliapfanzelter.bsky.social and some analysis by me.
doi.org/10.1101/2025...
Reposted by Joshua Hislop
3D reconstruction of a human Carnegie stage 9 embryo provides a snapshot of early body plan formation www.sciencedirect.com/science/arti...
3D reconstruction of a human Carnegie stage 9 embryo provides a snapshot of early body plan formation
The Carnegie stage 9 (CS9) embryo is a pivotal phase signifying the conclusion of gastrulation and the onset of early organogenesis, crucial for initi…
www.sciencedirect.com
July 8, 2025 at 10:21 PM
3D reconstruction of a human Carnegie stage 9 embryo provides a snapshot of early body plan formation www.sciencedirect.com/science/arti...
Reposted by Joshua Hislop
Have you ever thought about inflating tissues?
Or maybe quickly deflating those inflated tissues?
New #EpithelialMechanics pre-print: doi.org/10.1101/2025...
🧵 with pressure control, multiscale buckling, controlled wrinkling
Or maybe quickly deflating those inflated tissues?
New #EpithelialMechanics pre-print: doi.org/10.1101/2025...
🧵 with pressure control, multiscale buckling, controlled wrinkling
July 3, 2025 at 2:23 PM
Have you ever thought about inflating tissues?
Or maybe quickly deflating those inflated tissues?
New #EpithelialMechanics pre-print: doi.org/10.1101/2025...
🧵 with pressure control, multiscale buckling, controlled wrinkling
Or maybe quickly deflating those inflated tissues?
New #EpithelialMechanics pre-print: doi.org/10.1101/2025...
🧵 with pressure control, multiscale buckling, controlled wrinkling
Reposted by Joshua Hislop
While helping out on a cool genomics project recently, I came to realise I’d been taught a pretty big inaccuracy about the events that occur at fertilization. I suspect that almost everyone reading this has the same misapprehension, so let’s do some learning together: 1/
May 30, 2025 at 6:51 PM
While helping out on a cool genomics project recently, I came to realise I’d been taught a pretty big inaccuracy about the events that occur at fertilization. I suspect that almost everyone reading this has the same misapprehension, so let’s do some learning together: 1/
Reposted by Joshua Hislop
Beautiful work from @santoslab.bsky.social
A 3D model of human amnion from hESCs
These recapitulate amniotic sac development, revealing GATA3 as key amniogenesis driver & autoregulatory BMP/Wnt feedback loops
And check out the size of them in the video
www.cell.com/cell/fulltex...
A 3D model of human amnion from hESCs
These recapitulate amniotic sac development, revealing GATA3 as key amniogenesis driver & autoregulatory BMP/Wnt feedback loops
And check out the size of them in the video
www.cell.com/cell/fulltex...
Post-gastrulation amnioids as a stem cell-derived model of human extra-embryonic development
An embryonic stem cell-based 3D model that accurately recapitulates the size, morphology,
and functional features of the human amnion at 4 weeks, termed the post-gastrulation
amnioid (PGA), is develop...
www.cell.com
May 15, 2025 at 4:17 PM
Beautiful work from @santoslab.bsky.social
A 3D model of human amnion from hESCs
These recapitulate amniotic sac development, revealing GATA3 as key amniogenesis driver & autoregulatory BMP/Wnt feedback loops
And check out the size of them in the video
www.cell.com/cell/fulltex...
A 3D model of human amnion from hESCs
These recapitulate amniotic sac development, revealing GATA3 as key amniogenesis driver & autoregulatory BMP/Wnt feedback loops
And check out the size of them in the video
www.cell.com/cell/fulltex...
Reposted by Joshua Hislop
Our chromosome-scale genome assembly of Nanomia septata, a siphonophore - doi.org/10.1101/2025... . Much larger than many other cnidarian genomes, fewer chromosomes, and highly rearranged. No smoking gun for how they achieve complex colony-level organization. Great work Namrata Ahuja and friends!
May 13, 2025 at 7:46 PM
Our chromosome-scale genome assembly of Nanomia septata, a siphonophore - doi.org/10.1101/2025... . Much larger than many other cnidarian genomes, fewer chromosomes, and highly rearranged. No smoking gun for how they achieve complex colony-level organization. Great work Namrata Ahuja and friends!
Reposted by Joshua Hislop
How can biological systems anticipate future events? In our new paper with @jordiplam.bsky.social, we show how a simple genetic circuit can predict future trends through a simple (and perhaps widespread) mechanism @drmichaellevin.bsky.social @koseskalab.bsky.social www.biorxiv.org/content/10.1...
April 28, 2025 at 10:42 PM
How can biological systems anticipate future events? In our new paper with @jordiplam.bsky.social, we show how a simple genetic circuit can predict future trends through a simple (and perhaps widespread) mechanism @drmichaellevin.bsky.social @koseskalab.bsky.social www.biorxiv.org/content/10.1...
Reposted by Joshua Hislop
N2B27 media formulations influence gastruloid development https://www.biorxiv.org/content/10.1101/2025.03.15.643474v1
March 17, 2025 at 4:30 AM
N2B27 media formulations influence gastruloid development https://www.biorxiv.org/content/10.1101/2025.03.15.643474v1
Reposted by Joshua Hislop
Our next western/evening 🌙 session seminar is happening next week! Join us to learn about
human embryo models with @bailweatherbee.bsky.social
and
pancreatic organoids with @amandaanderssonr.bsky.social
🗓️ Thursday, February 20th at
⏰ 09:30 PST / 12:30 EST / 17:30 UTC / 17:30 GMT / 18:30 CET
human embryo models with @bailweatherbee.bsky.social
and
pancreatic organoids with @amandaanderssonr.bsky.social
🗓️ Thursday, February 20th at
⏰ 09:30 PST / 12:30 EST / 17:30 UTC / 17:30 GMT / 18:30 CET
February 14, 2025 at 4:04 PM
Our next western/evening 🌙 session seminar is happening next week! Join us to learn about
human embryo models with @bailweatherbee.bsky.social
and
pancreatic organoids with @amandaanderssonr.bsky.social
🗓️ Thursday, February 20th at
⏰ 09:30 PST / 12:30 EST / 17:30 UTC / 17:30 GMT / 18:30 CET
human embryo models with @bailweatherbee.bsky.social
and
pancreatic organoids with @amandaanderssonr.bsky.social
🗓️ Thursday, February 20th at
⏰ 09:30 PST / 12:30 EST / 17:30 UTC / 17:30 GMT / 18:30 CET
Reposted by Joshua Hislop
We have a CUB SCOUT EMERGENCY.
Space people, anybody able to help here?
Using social media for this bc
1) urgent
2) at a specific date and time
@philplait.bsky.social @astrochic73.bsky.social @astrokatie.com
Space people, anybody able to help here?
Using social media for this bc
1) urgent
2) at a specific date and time
@philplait.bsky.social @astrochic73.bsky.social @astrokatie.com
February 11, 2025 at 7:27 PM
We have a CUB SCOUT EMERGENCY.
Space people, anybody able to help here?
Using social media for this bc
1) urgent
2) at a specific date and time
@philplait.bsky.social @astrochic73.bsky.social @astrokatie.com
Space people, anybody able to help here?
Using social media for this bc
1) urgent
2) at a specific date and time
@philplait.bsky.social @astrochic73.bsky.social @astrokatie.com
Reposted by Joshua Hislop
Is the placenta is a source of, or merely a niche for, blood-forming #hematopoietic #StemCells? This Primer explores a @plosbiology.org study showing that the placenta does not directly give rise to hematopoietic stem cells. 🧪 Paper: plos.io/4hl3qZF Primer: plos.io/40R1VeE
February 7, 2025 at 10:18 AM
Is the placenta is a source of, or merely a niche for, blood-forming #hematopoietic #StemCells? This Primer explores a @plosbiology.org study showing that the placenta does not directly give rise to hematopoietic stem cells. 🧪 Paper: plos.io/4hl3qZF Primer: plos.io/40R1VeE
Reposted by Joshua Hislop
Announcing this upcoming conference:
GASTRULATION RELOADED:
Developing, engineering, and evolving the body plan
Join us as we explore the latest research on gastrulation and axis formation/elongation, featuring common, exotic, and synthetic embryos!
📅 14–17 October 2025
📍 Paris, France
GASTRULATION RELOADED:
Developing, engineering, and evolving the body plan
Join us as we explore the latest research on gastrulation and axis formation/elongation, featuring common, exotic, and synthetic embryos!
📅 14–17 October 2025
📍 Paris, France
January 28, 2025 at 8:14 AM
Announcing this upcoming conference:
GASTRULATION RELOADED:
Developing, engineering, and evolving the body plan
Join us as we explore the latest research on gastrulation and axis formation/elongation, featuring common, exotic, and synthetic embryos!
📅 14–17 October 2025
📍 Paris, France
GASTRULATION RELOADED:
Developing, engineering, and evolving the body plan
Join us as we explore the latest research on gastrulation and axis formation/elongation, featuring common, exotic, and synthetic embryos!
📅 14–17 October 2025
📍 Paris, France
Reposted by Joshua Hislop
It's actually theft of taxpayers money when websites go dark. We paid for that data
February 1, 2025 at 12:55 AM
It's actually theft of taxpayers money when websites go dark. We paid for that data
Reposted by Joshua Hislop
1/3 Embryogenesis requires fit cells. Here, following work from @miguel-torres-1963.bsky.social & @tristanrodriguez72.bsky.social and led by @joshifrenster.bsky.social we develop a #gastruloid model of #CellCompetition
www.biorxiv.org/content/10.1...
showing it recapitulates much of the embryo 🧵
www.biorxiv.org/content/10.1...
showing it recapitulates much of the embryo 🧵
January 26, 2025 at 4:36 PM
1/3 Embryogenesis requires fit cells. Here, following work from @miguel-torres-1963.bsky.social & @tristanrodriguez72.bsky.social and led by @joshifrenster.bsky.social we develop a #gastruloid model of #CellCompetition
www.biorxiv.org/content/10.1...
showing it recapitulates much of the embryo 🧵
www.biorxiv.org/content/10.1...
showing it recapitulates much of the embryo 🧵
Reposted by Joshua Hislop
Excited to present our new preprint clarifying forebrain developmental organisation. Complex growth patterns shape the forebrain but distort the A-P + D-V axes, hence prior confusion over brain layout. Big love for chicken embryos, simpler to interpret than mouse 🐣1/3 www.biorxiv.org/content/10.1...
Resolving forebrain developmental organisation by analysis of differential growth patterns
The forebrain is the most complex region of the vertebrate CNS, and its developmental organisation is controversial. We fate-mapped the embryonic chick forebrain using lipophilic dyes and Cre-recombin...
www.biorxiv.org
January 13, 2025 at 3:36 PM
Excited to present our new preprint clarifying forebrain developmental organisation. Complex growth patterns shape the forebrain but distort the A-P + D-V axes, hence prior confusion over brain layout. Big love for chicken embryos, simpler to interpret than mouse 🐣1/3 www.biorxiv.org/content/10.1...
Reposted by Joshua Hislop
For some time now we’ve been aware that Chiron #gastruloids, while representing well axial elongation, exhibit a shortage of anterior fates. Here www.biorxiv.org/content/10.1...
@Dias and colleagues uncover the reason and explain the implications for our understanding of mammalian gastrulation
🧵
@Dias and colleagues uncover the reason and explain the implications for our understanding of mammalian gastrulation
🧵
January 12, 2025 at 11:09 AM
For some time now we’ve been aware that Chiron #gastruloids, while representing well axial elongation, exhibit a shortage of anterior fates. Here www.biorxiv.org/content/10.1...
@Dias and colleagues uncover the reason and explain the implications for our understanding of mammalian gastrulation
🧵
@Dias and colleagues uncover the reason and explain the implications for our understanding of mammalian gastrulation
🧵