Wu et al. differentiate hPSC-derived Schwann cell precursors into functional parasympathetic neurons. These parasympathetic neurons are employed to study specific defects in the genetic autonomic diso...

A 50-year multi-tissue proteomic atlas reveals transcriptome-proteome decoupling, proteostasis decline with amyloid accumulation, and asynchronous aging clocks, identifying circulating senoproteins co...

Metabolic enzymes of the tricarboxylic acid cycle, such as 2-oxoglutarate dehydrogenase, are differentially expressed in absorptive and secretory lineages, guiding cell fate establishment and offering...

Developmental timing differs strikingly between mammals. All embryonic and some placental lineages emerge from the pluripotent epiblast in a temporally defined sequence, lasting about two weeks in the...

Much remains to be learned about the clonal fate of mammalian epiblast cells. Here, we develop high-diversity Cre recombinase-driven LoxCode barcoding…

Nature Biotechnology - Defining and benchmarking open problems in single-cell analysis

The Carnegie stage 9 (CS9) embryo is a pivotal phase signifying the conclusion of gastrulation and the onset of early organogenesis, crucial for initi…

Wang et al. uncover that aged muscle stem cells have blunted PGE2-EP4 signaling that is critical for their regenerative function. Treatment with exogenous PGE2 reverses age-related defects in muscle stem cells and imparts an epigenetic response that enhances their capacity for repair after injury and transplantation.

Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12.

In the liver, hepatocytes can convert to biliary epithelial cells (BECs) after injury. YAP/TEAD activation triggers chromatin changes during this process. HBO1, a protein that modifies histones, acts as a barrier to reprogramming by limiting chromatin accessibility. Depleting HBO1 accelerates conversion, making it a potential therapeutic target for cholangiopathies.

The liver and pancreas arise from a common developmental origin and are controlled by shared gene regulatory programs.These organs are central to two of the most common and devastating metabolic dise…

Placodes and the neural crest are defining features of vertebrates. In this study, we investigate their lineages in mice using in utero approaches. We demonstrated that nanoinjection at embryonic day ...